REPORT ON t THE PURITY OT ICE FROM Onondaga Lake, the Erie Canal at Syracuse, and from Cazenovia Lake. State Board of Health of New York, Albany, September 1st, 1886. To the Board of Health of the City of Syracuse, N. Y. : Gentlemen-At a meeting of the State Board of Health, held on the 31st ult., at Albany, the accompanying report by James. T. Garnier, Consulting Engineer, on the purity of ice from Ononcnaga Lake, the Erie Canal at Syracuse, and from Cazenovia Lake was submitted, and on motion was adopted as the report of the Board, and ordered trans- mitted to the Board of Health of Syracuse. Very truly, LEWIS BALCH, Secretary. REPORT ON THE PURITY OF ICE FROM Onondaga Lake, the Erie Canal at Syracuse, and from Cazenovia Lake. REPORT ON THE PURITY OF ICE FROM ONONDAGA LAKE, THE ERIE CANAL AT SYRACUSE, AND FROM CAZENOVIA LAKE. To Lewis Balch, M. D., Secretary of the State Board of Health: Sir-The question of the purity of the ice of Onondaga Lake having been referred to me, I took occasion at the meeting of the Sanitary Committee at Syracuse, on Decem- ber 8th, to have a hearing of ah parties interested in the matter. Ice for the use of Syracuse is furnished by several parties. Messrs. Sawmiller, Knapp, Listman, Gee Bros., and others are among the ice dealers. Ice for city use is cut from Onondaga, Oneida, Cazenovia, and Cayuga, and other lakes and from several points on the Erie Canal. It appears that the City Board of Health on the recom- mendation of Dr. Englehardt forbade Messrs. Sawmiller Bros, from selling, for purposes where it come in direct contact with food or drink, the ice cut from Onondaga Lake. This firm complained that such action was unjust as the ice sold by them was pure and no more subject to sanitary condemnation than much of the other ice sold in the city. The request for an examination of the subject by the State Board of Health comes from the City Board of Health of Syracuse. I had already made personal examination of the sanitary condition of most of the waters flowing into the southeast end of Onondaga Lake. Such additional knowledge as was needed I obtained from witnesses at the 6 hearing on December 8th. The principal stream entering Onondaga Lake in the City of Syracuse is Onondaga Creek, into which flow all the principal sewers of the city. An examination of the condition of this stream proves that its waters are very grossly polluted. Bear Trap Creek, another important affluent of the lake is probably comparatively pure. Onondaga is a small but deep lake. A large proportion of its water is derived from Onondaga Creek. The ice of the lake being made from water polluted with sewage is therefore open to suspicion ; but no positive judgment could be made of the degree of pollution of the ice with- out a series of analyses of samples cut from the lake at properly selected points, and analyes of samples of the water from the points where the ice was cut were also necessary. I therefore advised that the Committee recommend the City Board of Health of Syracuse to have a specimen of ice cut at the point where Onondaga Creek enters the Lake, another specimen two thousand feet north of this point, another two thousand feet northwest of the last point, and two more specimens within the next mile in a northwest- erly direction, and that specimens of the water of the Lake be taken at the points where the ice was cut. In partial accordance with these recommendations speci- mens of ice and water were taken from three points in Onondaga Lake, one in the wide, water of the Canal at the foot of Cherry Street, and the other in the center of Cazen- ovia Lake, three hundred feet from the east shore and in thirty feet of water. These specimens of ice and water have been analyzed by Professor Willis G. Tucker, of Albany, one of the analysts of the State Board of Health, and a biological examination made by Dr. William Hailes. Before attempting to discuss these results, it will be necessary to settle if possible certain general questions con- cerning the relations of impure ice to health. 7 RELATION OF IMPURE ICE TO HEALTH. There is a popular impression that water in freezing puri- fies itself ; and for this reason many streams and ponds throughout the country are used for cutting ice for domestic supply that would be considered unfit for furnishing whole- some drinking water. The first question therefore, that arises in connection with this matter in the minds of the people is this: CAN ICE FROM CONTAMINATED WATER CAUSE SICKNESS ? This is best answered by reference to the experience of competent observers. The European records from which so much sanitary knowledge is drawn, are silent on this point, because ice has there been so little used, and because the ice supply of that region is largely brought from America and the far north. In the United States the attention of the medical profession seems not to have been directed to impure ice as a possible source of disease till 1875, when a mild but well marked epidemic occurred at Rye Beach, N. H., which was very thoroughly investigated by Dr. A. H. Nichols, of Boston, and the results fully reported to the State Board of Health of Massachusetts. The proof was unusually conclusive that the epidemic disturbance of the digestive organs was due to ice cut from a shallow pond containing a large amount of decomposing organic matter. In Appendix " A " will be found the full report of this case, which is remarkable alike for being the first one on record, the most complete in its investigation, and the most conclusive in its proof that ice frozen from polluted water may cause disease. In the American Journal of Medical Sciences, January, 1878, Dr. C. Smart, Assistant Surgeon U. S. A., published the results of a most interesting investigation on, "Moun- tain Fever and Malarious Water," in which he traced the origin of Mountain Fever to the melting snow water in the Rocky Mountain streams and inferred that the germs of this typho-malarial fever were brought down from the 8 atmosphere by the snow, and, remaining frozen during the winter, passed into the streams with the melting snow in May, June and early July. In 1879, an epidemic dysentery occurred in the village of Washington, Conn., which was investigated by Dr. Orlando Brown, of Litchfield, and Dr. J. H. Raymond, of Brooklyn. The ice used by the persons attacked was from a small and much polluted stream. The analysis of the ice itself showed it to be grossly contaminated. No other origin being found for the epidemic, which was limited to those who used the impure ice, the ice was pronounced to be the cause of the dysentery. The case as reported by the State Board of Health of Connecticut is given in Appendix "B." In the Report of the Connecticut Board for 1882, an iso- lated case of typhoid is reported, where a private ice supply was cut from a pond which had received through a drain the dejecta of a typhoid patient. The details as reported are given in Appendix " 0." In Appendix "D" is given a quotation from the Report of the Connecticut State Board of Health for 1880, stating that several isolated cases of enteric trouble and one death were reported during that year, as due to free use of sewage polluted ice. The epidemic of Typhoid Fever at Plymouth, Penn., in the Spring of 1885, was investigated by many competent experts, and the accepted conclusion was that the epidemic was caused by the dejecta of a typhoid patient being thrown out upon the snow near the bank of a reservoir, from which part of the town was supplied with water. The germs of the disease remained frozen till the March thaw, when with the melting snow they passed into the reservoir. In Appendix "E" I have brought together quotations from a number of recognized authorities which show that the best sanitary authorities in this country are now con- vinced that ice from polluted water has caused disease. Although the cases quoted above vary very much in the completeness of the proof that impure ice caused the dis- ease in question, yet after a careful review of all the facts 9 reported and of the opinions of experts, it must be con- cluded that ice from polluted water has been the cause of disease, varying in intensity from mild disturbances of the digestive organs to fatal dysentery and typhoid fever. If then, ice may be the carrier of disease, the next im- portant question to be considered is TO WHAT EXTENT DOES WATER IN FREEZING FREE ITSELF OF IMPURITIES ? First as to chemical impurities. With the purpose of determining to what extent water in freezing frees itself from dissolved organic matter, Dr. C. P. Pengra, of Ovid, Michigan, made in 1882 a series of experiments with vari- ous solutions of crystalioids and colloids. The solutions of urea, grape sugar and arsenic in freezing were purified of from 40 to 55 per cent of the organic matter. The ice from solutions of Albumen was purified of 20 per cent of the organic matter in the water. Experiments by Prof. S. P. Sharpies, of Boston, in 1879, on the water and ice from three ponds and one much pol- luted pond hole near a slaughter house, show the ice from the ponds to have contained from 17 to 40 per cent of the "free and albuminoid ammonia" found in the water from which it was frozen, while the ice from the much polluted pond hole had only 8 per cent of the organic impurity of the water. The details of these investigations are given in Appendix "F." In the case of Rye Beach the water from the contamin- ated ice, when analyzed after filtering, so as to measure only the organic mater in solution, contained of "free ammania," .0231, while the water from the pond contained .0197. Of "albuminoid ammonia," the amounts were .0165 and .0597. From this it would appear that the ice contained 25 per cent of the dissolved organic impurities of the water, but this is somewhat uncertain, as the ice was cut from the pond in winter and the water for analysis was taken the following summer. In the case of the Pittsfield ice supply, given in Appendix 10 " E," the pond water seems to have contained of " free am- monia" about .020, and the ice from it, .007. Of "albu- minoid ammonia," the water .015 and the ice, .0013. All of the fractions here given denote parts per 100,000. The table of analysis of Onondaga Lake waters and ice hereafter given, shows approximately the following re- sults :- Free Ammonia, in parts per 100,000. Albuminoid Ammonia Onondaga Lake Water, .0252 .0113 " " Ice, .0032 .001 Percentage of Organic Im L- purity Retained in Ice, 12 per cent. 10 per cent. The analysis of water and ice from the canal show that the ice contains about 20 per cent of the dissolved organic impurity of the water, and from Cazenovia Lake the ice contains about 21 per cent of the dissolved organic matter in the water. These facts prove conclusively that water in freezing frees itself of only a part of the organic matter dissolved in it, and that ice may contain from 10 to 80 per cent, of organic impurities in solution in the water from which it was frozen. IMPURITIES IN SUSPENSION IN WATER. Besides the organic matter dissolved in water, it may contain a very large amount of organic matter in suspen- sion. In the Rye Beach case it appeared that only about 20 per cent, of the impurities in the ice had been in solution in the water. Four times as much suspended organic matter had been caught in the ice in freezing. Before filtering, the water from the ice contained of Albuminod Ammonia, .0704, and only .0165 after filtering. In Appendix " G " is quoted from the Report of the State Board of Health of New Hampshire for 1882, an account of the pollution of the water and ice of rhe Ammonoosuc River for twenty miles below the tanneries and glove factories of Littleton. Ice cut ten miles below Littleton 11 contained so much hair and so many particles of flesh, that in melting a large cake of it an intolerable odor would be produced. Hair was visible in the stream twenty miles be- low the tanneries. Ice may therefore be grossly polluted by organic matter floating in the water from which it was frozen. PURIFACTION FROM LIVING ORGANISM. Chemical analysis can determine only a part of the im- purities of ice or water. The micro-organism which swarm in polluted waters can be detected only by the expert biolo- gist working with the microscope. While many of these organisms are harmless to health, others are endowed with powers terribly destructive to life. Some six or seven of the zymotic diseases, and possibly consumption, have been proven to be caused by various species of bacteria, living plants, whose minute spores may float by myriads in air or water, invisible except through the higher powers of the microscope, and undiscoverable by chemical analysis. Their powers of self multiplication are so great that they may in- crease a million fold in a few hours. Koch's researches have shown that the bacillus which produces Asiatic Cholera thrives in water under favorable conditions and multiplies itself in untold numbers. English sanitary observers have long held as the result of their ex- perience that water was the great carrier of Cholera and Typhoid Fever. Experience in this country has been the same. In both Europe and America there are many cases on record to show that an extremely small quantity of the dejecta of a typhoid or cholera patient may infuse the poison through millions of gallons of water and spread pestilence through a community. There is every reason to suppose that this probable power in minute quantities of typhoid and cholera poison to fatally pollute comparatively large bodies of water, is due to the action of a living organ- ism multiplying itself under favorable conditions with marvelous rapidity. That they do not always so multiply, is due to conditions of which little is understood. Some- 12 times it is probable that one class of bacteria destroys an- other. Sometimes the proper food and temperature for the development of the plants are not present. The disease- producing germs are known to exist in great numbers in the excreta of persons suffering from a specific disorder. Hence it is that sewage is believed to be so dangerous a menace to any water supply. In every city there are many patients suffering from infectious diseases, the specific germs of which must pass into the sewers where heat, moisture, darkness and the presence of ammonia furnish conditions under which most bacteria thrive. When these pathogenic germs are carried out into a stream or lake they may either remain dormant, be destroyed, or, under pecu- liar and favorable conditions, they may multiply enormously and render the water extremely dangerous for drinking. Experience has shown that a sewage contaminated water supply, usually comparatively harmless, may suddenly, owing to some unknown change of conditions, become the carrier of the seeds of a fatal epidemic. For this reason all water known or liable to contain the specific germs of disease is now considered unfit for potable purposes if a better can be had. This conclusion has been recognized by the law power of the State and a statute enacted to "Protect Public Water Supplies from Sewage Pollution." Regarding ice the question then arises does water in freez- ing free itself from living organisms, and will such organ- isms if frozen into ice have their vitality destroyed. The experiments of Dr. C. H. Pengra given in Appendix "H " show that under the conditions which he produced in his labratory the ice contained ten per cent of the living organisms which occurred in the water from which the ice was frozen. In the same Appendix will be found Prof. Leidy's account of his finding in ice used for domestic purposes, "worms, immature Anguillulas, and a number of Rotifer Vulgaria, all living. " Prof. Kendrick of Glasgow, made a number of experi- ments to determine whether solutions could be sterilized by 13 freezing. "The organic fluids were exposed to tempera- tures more than 120 deg. below 0 deg. F., but on thawing they were found to contain living organisms still." Ice therefore is proven to contain a considerable percent- age of the bacteria which were in the water from which it was frozen, and the vitality of many of these organisms is not destroyed by the process of freezing. CONCLUSIONS RESPECTING ICE POLLUTION. The facts above given, warrant, it is believed, the follow- ing conclusions: ' First. Ice frozen from impure water has caused illness. Second. Ice may contain from 8 to 10 per cent of the or- ganic matter dissolved in the water from which it was frozen. Third. Ice may contain, in addition to the dissolved im- purities, a very large amount of organic matter which had been suspended or floating in the water before freezing. Fourth. Ice may contain living animals and plants from the size of a visible worm down to the minutest spores of bacteria and the vitality of these organisms be unaffected by freezing. THE ICE OF ONONDAGA LAKE, THE ERIE CANAL AT SYRACUSE, AND CAZENOVIA LAKE. ONONDAGA CREEK. The City of Syracuse is situated at the south-east end of Onondaga Lake, which extends to the north-west nearly five miles. The breadth of the lake is from one-half to three quarters of a mile. At a short distance from the shore the lake is quite deep at the south-west end. The great body of the water in this upper end of the lake comes 14 from Onondaga Creek, a large stream which enters near the south-east corner. The course of the creek for the last three miles is through the City of Syracuse, where all the sewers empty into it. The flow of the stream is probably from 10 to 25,000,000 gals, per diem, and its fall through the city about one foot per thousand. In examining the banks of the creek December 9th, 1884, I observed that so much sewage was then poured into the stream that deposits of filth were forming on its banks and in its channel. The slight fall, and reduced summer flow render the current sluggish and incapable of carrying away the greater part of the solids that come down with the sewage. They are therefore deposited on the bottom and sides of the creek. Several causes are acting to make the stream fluctuate. This rise and fall of the water tends to alternately spread the sewage over the banks and shallows of the channel, and then, with the subsidence of the water, to expose it to the action of the sun and air. At the time of my inspection there was a margin of from one to four feet wide of black, putrefying organic matter exposed along the shores. At places the organic matter in the bed of the creek is undergoing rapid decomposition and giving off the gases of putrefaction which rise in bubbles to the surface. Probably 5,000,000 gals, of sewage are daily poured into Onondaga Creek which in time of low water is liable to have a flow of not over 10,000,000 gals, per diem. The whole flow of the stream is thus converted into dilute sewage, from which the heavier matter is deposited when the current is sluggish, while the remainder flows a mile below into Onondaga Lake. In time of flood a part of the decomposing filth of the channel is scoured out and carried down into the lake, there to be deposited and continue the process of putrefaction. Probably two thirds of the water of the upper half of the lake flows in from this open sewer of Onondaga Creek. 15 WATER ANALYSIS. In the following tables are given the analyses of speci- mens of ice and water taken from the same spot at the same time for the sake of comparison. They were taken by an officer of the City Board or Health from points where ice was being cut for the several dealers named, as is certi- fied by the affidavit given in Appendix "I." In Appendix "I" is given the report of Dr. Willis G. Tucker, the Chemi- cal Analyst. Specimen No. 3 was taken about 1,500 feet from the mouth of Onondaga Creek and 1,200 feet from the shore ; No. 1 was taken 3,000 feet from the mouth of the creek, and 1,200 feet from shore ; No. 2 is from a point two miles from the mouth of the creek and 600 feet from the shore. It may be said with reference to the biological examina- tion, that the method used of adding some of the water to be tested to sterilized gelatine and noting the time when, owing to the development of bacteria, the gelatine begins to liquefy and the extent of the liquefaction on different days, does not give a true measure of the number of micro- organisms in water, for of the forty-nine species of bacteria described by Dr. James Eisberg in his Bakteriologische Diagnostik, only fifteen species liquefy gelatine. Among those which do not liquefy gelatine is the organism which is supposed to be the cause of typhoid fever. The liquefac- tion method while it is sufficiently accurate for determining approximately the relative numbers of bacteria in the water and ice is not a measure of the absolute number. The results indicated by it would however be too small rather than too large. As regards the large amount of Chlorine shown by the chemical analysis, it is due principally to the water of the salt wells which run into the lake. 16 Description of Sample. Color and Appearance. Odor at 100 deg. F. Chlorine in Chlorides. Free Ammo- nia. Albuminoid Ammonia. Total solid Residue. Loss on Ig- nition. Mineral Matter. Oxygen absorbed at 80 deg. Fah. Conclusions. In 15 min. In 4 hrs. Sawmiller, Onondaga Lake Water, No. 1. Light brownish green, slightly turbid; very slight sediment. Slight. 24.50 0.0226 0.0095 86.00 20.00 66.00 0.1093 0.2133 Bad. Listman, ' Onondaga Lake Water, No. 2. Brownish green tint; slightly turbid ; slight flocculent sed- iment. Slightly disa- greeable. 17.80 0.0210 0.0150 66.50 15.50 51.00 0.1693 0.2080 Bad. Gee Brothers, Onon- daga Lake Water, No. 3. Greenish tint; slight flocculent sediment. Slight. 23.60 0.0320 0.0095 85.00 18.50 66.50 0.0853 0.1866 Bad. Knapp, Wide Waters Erie Canal, No. 4. Yellowish tint; slight sediment. Slight. 0.74 0.0160 0.0080 49.00 14.00 35.00 0.1066 0.2000 Bad. Listman, Cazenovia Lake Water, No. 5. Greenish tint; slight sediment. Slight; pecu- liar. 0.24 0.0047 0.0070 14.50 7.00 7.50 0.0933 0.2133 Doubtful. Sawmiller, Onondaga Lake Ice, No. 1. Very light greenish tint; very slight sed- iment. Slightly disa- greeable. 0.18 0.0020 0.0010 2.00 1.40 0.60 0.0133 0.0586 Good. Listman, Onondaga Lake Ice, No. 2. Very light greenish tint; very slight sed- iment. Slightly disa- greeable. 0.24 0.0053 0.0010 1.80 1.40 0.40 0.0160 0.0400 Not completely satisfactory. Gee Brothers, Onon- daga Lake Ice, No. 3. Very light greenish tint; very slight sed- iment. Slight. 0.20 0.0027 0.0010 1.20 0.80 0.40 0.0240 0.0466 Good. Knapp, Wide Waters Erie Canal (Ice), No. 4. Very light greenish tint; very slight sed- iment. Slight. 0.15 0.0033 0.0015 1.10 0.80 0.30 0.0213 0.0480 Good. Listman, Cazenovia Lake Ice, No. 5. * Very light blueish green tint; no sed- iment. Slight. 0.18 0.0013 0.0010 1.20 0.90 0.30 0.0106 0.0266 Good. Analyses oe Syracuse Waters and Ice, March 20, 1886. Results parts per 100,000. Willis G. Tucker. Table I. 17 Table II. REPORT OF BIOLOGICAL EXAMINATION. Pathological Laboratory, Albany Medical College. Secretary State Board of Health: Dear Sir-Upon biological analysis of samples of waters and ices sent from Syracuse, N. Y., I would report as follows: Locality. Date of Culture. Time of first Ap- pearance of Mi- cro-organisms. Time of Begin- ning Liquefac- tion of Gela- tine. Extent of Lique- faction of Gela- tine. Observations, etc. No. 1.-Water. March 9. March 22. If days. days. inch. 8 days. Bacteria exceedingly numerous and de- structive of gelatine -(bad.) No. 1.-Ice. March 9. March 22. H days. 21 days. 2 inch. 8 days. Destructive of gela- tine; organisms very numerous-(bad.) No. 2.-Water. Alarch 9. March 22. 1| days. 2J days. 1-J- inch. 8 days. Organisms very nu- merous-(bad.) No. 2.-Ice. March 9. March 22. 1| days. 2£ days. 14 inch. 8 days. Organisms very nu- merous, freezing de- lays destruction, de- velop slowly. No. 3.-Water. March 9. March 22. 1| days. 2J days. 1J inch. 8 days. Organisms very nu- merous. No. 3.-Ice. March 9. March 22. 1£ days. 2| days. 1 inch. 8 days. Organisms very nu- merous, but very small, and develop slowly. No. 4.-Water. March 9. March 22. 1| days. 24 days. 1 inch. 8 days. Organisms numerous and small. No. 4.-Ice. March 9. March 22. 14 days. 24 days. 1 inch. 8 days. Organisms numerous and small. No. 5.-Water. March 9. March. 22. days. 2J days. 4 inch. 8 days. Numerous organisms, large and small. No. 5.-Ice. March 9. March 22. 1-J- days. 2i days. i inch. 8 days. Numerous organisms, large and small, but develop slowly. All the above samples of water and ice show the presence of bacteria in great abundance-freezing does not destroy or remove them, but retards their growth somewhat. Bio- logically considered, all the above samples would be ranked as unsafe waters for potable purposes. WILLIAM HAILES. 18 The chemical analyses above given show that the waters from each point in Onondaga Lake are polluted as well as that from the canal. The following are the amounts of free and albuminoid ammonia in the lake samples arranged ac- cording to their nearness to the mouth of Onondaga Creek : Free Ammonia. Albuminoid Ammonia. No. 3. 1500 ft. from creek .032 .0095 No. 1. 3000 ft. from creek .0226 .0095 No. 2. 10000 ft. from creek .021 .0150 There seems little to choose between them on chemical grounds. They are all polluted and from the sanitary sur- vey above reported the polluting matter is known to be largely sewage. The analyses of the ice show that the ice of Onondaga Lake contains probably from ten to twelve per cent of the sewage impurities dissolved in the same quantity of unfrozen water of the lake. Arranged in the order of nearness to Onondaga Creek the ice samples stand as follows : Free Ammonia. Albuminoid Ammonia. No. 3. 1500 ft. from creek .0027 .0010 No. 1. 3000 ft. from creek .0020 .0010 No. 2. 10000 ft. from creek .0053 .0010 There is little to choose between them on chemical grounds although that most distant from the creek has the largest amount of free ammonia. While the ice has freed itself from 90 per cent of the solu- ble filth of the water from which it was frozen ten per cent of the dissolved sew'age is retained in the ice. The ice of the whole lake is probably to that extent sewage polluted. But it contains also matter far more dangerous than that in solution and detectable by chemical analysis. The biolog- ical examination shows the presence of bacteria in great abundance in the water and in the ice. Here again there is so little to choose between the various samples that Dr. Hailes pronounces them all as unsafe for potable purposes. It is by some contended that wholesome waters may con- tain large numbers of bacteria. This is probably true, be- cause many species of bacteria are harmless. But where great numbers of bacteria are found in waters coming from 19 a source known to supply large quantities of disease-pro- ducing or pathogenic species or their spores, then the pres- ence of numerous micro-organisms is strongly indicative of danger. CONCLUSIONS. From such clear and positive evidence but one conclusion can be reached. Onondaga Lake is contaminated with sewage and this contamination will continue to increase. The water con- tains numerous bacteria or their spores. The sewers of Syracuse are bringing down into the lake gBeat numbers of these micro-organisms. Among them is a varying percent- age of pathogenic germs. The ice of the lake in freezing takes up a portion of the bacteria brought down by the sewers. It therefore doubtless contains its proportion of the disease-producing germs from the Syracuse sewers. This cannot but be harmful to some susceptible persons at all times, while under certain unknown conditions they may rapidly multiply or increase in virulence and spread disease and death throughout the community. The danger from Onondaga ice is certainly real and in creasing. Syracuse can obtain an ample supply of ice of unquestioned purity from other points within the reach of the city. I am, therefore, of the opinion that the ice from Onondaga Lake should not be used for any purposes where it is liable to come in contact with food or drink. WIDE WATER OF ERIE CANAL AT SYRACUSE. Chemical analysis shows the water of the canal to be im- pure, and from inspection, the impurities are known to be from human rather than from natural sources. The canal ice contains some twenty per cent of the organic impurity of the water from which it was frozen. Both water and ice contain numerous bacteria or their spores. As better can be had there seems no good reason why this ice should be used for potable purposes. 20 CAZENOVIA LAKE. Chemical analysis shows the water of Cazenovia Lake to contain less than half the "free and albuminoid ammonia" in the canal and only one-third of that in Onondaga Lake. While both the ice and water of the lake are shown to con- tain numerous bacteria, there is no evidence submitted to me to prove that these bacteria or spores are from danger- ous sources. There does not therefore, in my judgment, appear to be sufficient evidence to condemn the use of ice from Cazenovia Lake. ' Very respectfully submitted, JAMES T. GARDINER, Consulting Engineer. Albany, July 19th, 1886. APPENDIX "A." Report on an Outbreak of Intestinal Disorder, Attribu- table to the Contamination of Drinking-Water by Means of Impure Ice. By A. H. Nichols, M. D., of Boston. Rye Beach is an attractive and popular seaside resort upon the coast of New Hampshire, about fifteen miles distant from the north-eastern corner of Massachusetts; during the month of July and August of each year it is thronged with visitors from the large cities. At the beginning of the season of 1875, there broke out among the guests of one of the large hotels of this place, a somewhat extensive, though comparatively mild epidemic. Being the only practicing physician in the vicinity, I was requested by the proprietors of the hotel to make a detailed investigation as to the causes of the disorder. The results of this examination revealed a novel and commonly unsus- pected source of contamination of drinking-water, and they have, therefore, seemed to me worth communicating to the Board of Health. The disorder in question may be comprehended under the general term disturbance of the digestive system, characterized by a sensation of giddiness and nausea, vomiting, diarrhoea, severe abdominal pain, all of which was accompanied by fever, loss of appetite, con- tinued indigestion, and mental depression. The epidemic, although confined within very limited boundaries, baffled for a considerable time all efforts to trace the trouble to any 22 specific cause; while the origo mali, when ultimately detected, proved to be contained in an article of ordinary consumption, usually considered as above suspicion as regards innocuousness. The first few cases coming under observation did not attract particular attention, inasmuch as the symptoms manifested did not differ essentially from those noticed among the visitors in previous years, and induced by drinking well-water of the place, which, especi- ally when the wells are low, is strongly impregnated with sulphate of lime, carbonate of lime, and magnesia. It very soon became apparent, however, that the trouble was limi- ted to the inmates of a single hotel, accommodating about 800 guests, whereas the occupants of another public house, containing rooms for about 200, and distant but one-eighth of a mile, were enjoying an absolute immunity from all illness ; nor was any similar trouble known among the neighboring cottages, containing at least 500 visitors. This peculiar grouping of the patients rendered it, there- fore, tolerably certain that the whole disorder must be referred to some specific, local origin, to be sought for in the immediate vicinity of the hotel; and popular opinion pointed very strongly, from the outset, to the drinking- water. This was drawn from several wells, all sunk in an elevated ridge, and safely removed from drains, cesspools, dung-heaps, or other source of pollution. It was also ascertained, upon inquiry, that, in some instances, those persons affected, having apprehended trouble from the use of the water, had carefully limited themselves since their arrival to other beverages, but, as afterwards transpired, had not hesitated to use ice, either melted or otherwise. With respect to the drainage of the house, it appeared that during the previous winter the services of competent engineers from Boston had been secured, under whose super- vision an elaborate and complete system of sewerage had been recently constructed, by means of which all the dis- charge from the various sinks and water-closets was con- veyed directly into the ocean. The point of discharge of this sewer was at a safe distance from the house, while the sewer itself was securely trapped and ventilated in such a 23 manner as to preclude the idea of the escape of any foul gas within the house. Attention was next directed toward the cooking utensils, but all the articles pertaining to the kitchen were found to be scrupulously clean, nor did it appear that any agent or utensil was employed in the preparation of the food which would in any way tend to produce the symptoms com- plained of. Furthermore, the milk-supply was investigated, and found to be of unquestionable purity. The process of elimination was in this manner continued, until at length suspicion became directed to the supply of ice furnished to the house. It may be mentioned at this point, that a large portion of the ice consumed in this town is gathered from shallow ponds, formed during the winter by the flooding of meadows, and, therefore, contains as a rule, more or less grass and other vegetable matter, and is consequently far less transparent than the article commonly supplied in our large cities. I was not particularly sur- prised, then, to find that the ice in this case was rather impure and opaque, and that it contained numerous foreign substances varying in size, and apparently of vegetable origin. The theory that the outbreak, now increasing in extent and severity, was dependent upon the ice supply, was sud- denly strengthened by some pretty direct evidence, of which the following examples may be given : 1. A resident of the place, upon being questioned upon the subject, volunteered the testimony that during the previous winter he had taken home some ice from the same pond where the ice supply of the hotel was obtained, and having consumed a portion with the view of testing it, had experi- enced nausea and distress for the remainder of the day, which led him to decide that it was unfit for use. 2. Several persons affirmed that they detected a decidedly disagreeable odor emanating from the ice as it melted. 3. Two gentlemen having taken a quantity of ice with them upon an excursion, and drunk the water formed from it, were made violently ill. 24 4. The atmosphere of the house in which the suspected ice was stored was found to be decidedly offensive. 5. When some of the melted ice water was poured into a glass, and held in front of a dark-colored object, a strong light striking the glass from one side, it was found to be de- cidedly discolored, and charged with suspended matter. A visit was now made to the pond, and the condition of things here found removed all doubt as to the exceptional foulness of the water from which the ice was frozen. This pond is a flooded marsh, of irregular outlines, about two-thirds of a mile in length, and varying in width from 200 to 300 feet, with a uniform depth of about two feet. The source of the water supply was a small brook entering the lower end of the pond (bringing down all the sawdust from two neighboring saw-mills), and several springs said to be situated at the upper end. There had formerly existed an artificial channel, by means of which was maintained a direct communication between the pond and the ocean ; but for the past two years this channel had been filled up with sand and stones thrown up during heavy storms by the ac- tion of the sea, which drives in here with extreme violence, Of late, therefore, the water of the pond has become prac- tically stagnant, although a small quantity constantly per- colates a bank of gravel separating the pond from the ocean. A glance at the lower end of the pond was sufficient to demonstrate the source of the foulness of the water, for at this point, a space of about 500 feet long and 150 feet wide, directly in front of the mouth of the brook, was occupied by a homogeneous mass of putrescent matter, composed of marsh mud and decomposing sawdust. The water in the vicinity of this bank was discolored black, and when stirred up emitted an intolerably offensive odor. Several large houses are situated at no great distance from this end of the pond, the occupants of which, upon being questioned, asserted that when the water was stirred up by the rowing of boats, or ruffled by a wind blowing in the direction of the houses, the air was not unfrequently polluted to such an ex- tent as to render it necessary to dose the windows. Of course there could be no question but that this foul matter 25 held in suspension in the water was conveyed by currents and winds to every part of the pond, and in sufficient quan- tity to render the water in every part absolutely unfit for drinking purposes. In order to obtain further evidence as to the admixture of this foul matter with the ice, a quantity of the ice having been cleansed from all surface impurities was placed in a tub to melt, and the water thus obtained was poured into a fresh demijohn, sealed and forwarded for analysis to Prof. W. R. Nichols, who reported as follows : ' ' The water contains in suspension a considerable quan- tity of vegetable matter more or less decayed, and possesses a slightly disagreeable odor, which becomes more evident if the water is warmed. "Of the organic matter which is suspended in the water, and which may be removed by filtration, a portion, consist- ing of the larger and heavier particles, settles somewhat readily. Another portion, being more finely divided, re- mains for an indefinite time diffused through the water, and would be drunk by any one using the ice in the ordin- ary way. "I do not think it unreasonable to suppose that the pres- ence of this decaying organic matter may have been instru- mental in bringing about the unpleasant results you have observed." A sample of water was likewise taken for examination from the pond in question. This sample wa£ obtained from the central portion of the pond, in the early morning, when no air was stirring, and the water being quite calm and un- disturbed by undercurrents, the greater portion of any mat- ter held in suspension would at that time be precipitated to the bottom of the pond. Consequently, the sample taken would represent the purest water obtainable from the pond under any circumstances. The numerical results of these analyses are herewith ap- pended. For the purpose of affording a means of compari- son, there are also presented the results of analyses of a sample of ice supplied by the Boston Ice Company, and of the Cochituate water. 26 The subjoined sketch of the pond (drawn by Edward K. Clark, C. E.,) will serve to convey an idea of the general outline and character of the pond, the location and relative extent of the bank of sawdust and marsh mud by which the water was fouled, and the portion of the pond from which the ice was taken. The proprietors of the hotel, impelled by a proper sense of the responsibility resting upon them, rendered willing assistance in ferreting out the source of the trouble, and as soon as suspicion was directed towards the ice, its further use was promptly prohibited. Coincident with this disuse of the ice, there was observed an abrupt amelioration in the systems of nearly all who had hitherto been ill, while dur- ing the remainder of the season, no fresh cases of this char- acter are known to have occurred. The evidence thus collected seems to render it almost cer- tain, that the illness in question was induced by the con- sumption of ice contaminated by decomposed organic mat- ter. As to the actual or relative number of those made ill in this manner, no exact estimate can be made, for the symp- toms were, as a rule, not sufficiently severe to necessitate the aid of a physician. As an approximate estimate of the extent of the mischief produced, it may be said in round numbers, that the ice was consumed in variable quantities during a period of six weeks by five hundred individuals. Of these, twenty-six adults were known to manifest grave, continued, and char- acteristic symptoms. A large number, probably the ma- jority, of the guests drank the contaminated water with apparent impunity. In some, although decided illness was induced during the first two or three days after their arrival, an habituation to the water seemed to be afterwards acquired, and they enjoyed a subsequent immunity from all trouble. In the case of several, on the other hand, the stomach seemed to resent with ever-increasing emphasis the presence of the foul water, nor was relief obtained until after the patients had quit the place. Thus many who had come from a long distance with the hope of strengthen- 27 ing and improving their physical condition, returned home depressed, and without even having preserved the health they previously had. It is worthy of remark, that no person under the age of ten was known to be affected by the impure ice. Great responsibility devolves upon those wdio undertake to provide food and drink for large numbers of individuals, and to such the above experience inculcates the importance of giving especial attention to the purity of the drinking- water, and guarding against every possible source of con- tamination. The notion that ice purifies itself by the process of freez- ing, is not based upon trustworthy scientific observation. On the contrary, it is utterly wrong in principle to take ice for consumption from any pond the water of which is so fouled as to be unfit for drinking purposes. Numerical Results of Analyses. COMPONENT PARTS. Ice taken from Contaminat- ed Pond at Rye Beach.* Ice Supplied by Boston Ice Company, f Results Expressed in Parts, per 100,000. Results Expressed in Grains, to U. S. Gallon. Results Expressed in Parts, per 100,000. Results Expressed in Grains, to U. S. Gallon. Unfil- tered. Fil- tered. Unfil- tered. Fil- tered. Unfiltered. Filtered. Ammonia 0.0208 0.0213 0.0121 0.0124 0.0045 0.0026 Albuminoid ammonia 0 0704 0 0165 0.0410 0.0096 Inorganic matter 7.80 6.88 4.55 4.01 0.45 0.26 Organic and volatile matter. 5.72 2.84 3.33 1.66 0.31 0.18 Total solid residue, at 212 deg. Fahrenheit 13.52 9.72 7.88 5.67 0.76 0.44 Chlorine 3.23 1.88 Trace, leas than 0.02 Less than 0.012 Oxygen required to oxidize organic 0.334 0.495 0.033 0.019 * Small amount of nitrates. j- Trifling amount of suspended matter. J Determined by permanganate of potash. 28 Numerical Results of Analyses-Continued. COMPONENT PARTS. Wateb taken from a Pond at Eye Beach.* Cochituate Water. Mean of a Number of De- terminations. Results Expressed in Grains, to U. S. Gallon. Results Expressed in Parts, per 100,000. Results Expressed in Grains, to U. S. Gallon. Ammonia 0.0197 0.0115 0.0020 Albuminoid ammonia 0.0597 0.0348 0.0068 Inorganic matter 64.96 37.88 1.61 Organic and volatile matter 8.00 4.66 1.22 Total solid residue, at 212 deg. Fah- renheit 72.96 42.54 2.83 Chlorine 34.00 19.83 0.18 Evilalent to chloride of sodium 56.03 32.68 Oxygen required to -.oxidize organic matter 1.28 0.75 * Residue blackens strongly when heated. Evidently a large amount of organic matter. (Extract from Seventh Annual Report of the State Board of Health of Mas- sachusetts, January, 1876. From page 467 to 474, inclusive.) APPENDIX "B." Sickness From Impure Ice. The following data, are very kindly furnished by Dr. Orlando Brown, of Washington, Litchfield Co., under whose care the greater number of these cases were. The subject is a very important one, and all clear instances should be recorded, to prevent similar occurrences. The town of Washington possesses the usual topographi- cal features peculiar to Litchfield County, with perhaps less swamp land and stagnant water than the towns in the immediate vicinity. Thus far it has enjoyed complete im- 29 munity from malarial diseases, which is indeed the case with all but one or two towns in the county. There has been no epidemic form of disease of any kind for several years past. The local conditions of the case are as follows : The house is situated in a little valley among the highest hills of the region. The occupants were farming people of intelligence, the head of the family quite prominent in the public affairs of the town. The family consisted of the man and his wife, aged respectively 51 and 46, the wife's mother, aged 69, two children-a boy of 12 and a girl of 14 years-a laborer employed on the farm, and a woman employed to do gen- eral housework. There had been no sickness in the family previous to August 6, 1879. The boy was then attacked apparently with a mild form of dysentery. There had been during the summer in different parts of the town here and there a few cases of dysentery, otherwise no unusual prev- alence of intestinal diseases. The dejections were fre- quent of bloody mucous, without faecal matter, tenesmus was marked, temperature never rose above 100, pulse about 104. August 7th, the father became similarly affected, the dejections presenting the general appearance of beef brine. August 12th, the daughter was attacked, being seized with a chill followed by a temperature of 105°, pulse 130-140, nausea and vomiting. August 12th, the grandmother was also affected, the onset similar to that of the girl. Collapse came on as suddenly and as markedly as in Asiatic cholera. The girl died on the fifth day after seizure, the grandmother on the seventh, the boy on the ninth. The father, after a slow and tedious convalescence, recov- ered. The mother and house servant had persistent diarrhoea, controlled with difficulty, but no dysentery. The farm laborer was early frightened, and left the town. No report of his illness was ever received. The man that took his place went home at the end of a week, sick with dysentery, but recovered in about ten days. No cases occurred in his family or neighbors. 30 A sister of the wife that came to assist in the care of the sick was seized with dysentery, but recovered after six weeks' severe illness. Her children were ordered removed, but the two youngest, that were constantly with their mother during the day before removal, were attacked on the same day with a mild form of dysentery. The cause was evidently local, the type of the disease once established mildly contagious. The following facts as to the cause are obtained from the report of Dr. Raymond, of Brooklyn, N. Y.: Examination of the spring used to obtain drinking water excluded that as a possible source of the disease. The sur- roundings of the spring were unquestionably good, and analysis of the water as received in the house showed it to be of exceptional purity. The window curtain was exam- ined for arsenic, but no trace of mineral coloring matter found. The cellar was very damp, and the soil beneath and immediately adjoining the house damp from the free water supply brought into the house from the spring-five pints per minute. In case of heavy rains, water runs into the cellar through the wall. How much this water is contami- nated from the privy vault is not easily estimated. The vault had not been emptied for twelve years, and was far from being full, hence there must have been considerable soil saturation, as the privy was constantly used. The stream, from which the ice suspected was gathered, runs through a field along side the road. This field has for fifteen years been used as a running place for pigs, and swine were wallowing in the stream at the time it was examined. The ice water on analysis showed : Free ammonia, parts per million 08 Albuminoid ammonia, parts per million 09 The water was of a greenish color, with light colored organic particles in suspension. The stream also apparently receives drainage from house waste, and possibly sewage from the privy-vault before mentioned. The analysis and 31 general character of the ice water show sufficient cause for the production of the symptoms described. The ice water at Rye Beach contained considerable less ammonia. Albuminoid ammonia is a reliable indication of contami- nation when excessive. When accompanied with but little free ammonia, and no evidence of chlorine, its presence indicate vegetable decay, the products of which contaminate the water. When the albuminoid ammonia amounts to .05 parts per million, the quantity of free ammonia that accompanies it must be considered in estimating the amount of contamination.* A large percentage of albuminoid ammonia may exist, .10 per million even, if there be no free ammonia present. The presence of the chlorides indi- cates contamination from animal decay, when present with the forms of ammonia. Taken together, the large percent- age of both free and albuminoid ammonia in the ice water proves the excessive contamination of the water from which the ice was collected. (Taken from the Second Annual Report of the State Board of Health of the State of Connecticut, for the fiscal year ending November 31, 1879. Pages 90 to 92 inclusive.) APPENDIX "C." * * * * Freezing is a form of crystalization, and the general rules apply so far as inorganic pollution is in question. Ice frozen from water holding in solution min- eral salts, will contain less of these than the water from which it was frozen, and by repeatedly dissolving in dis- tilled water and refreezing, nearly all inorganic impurities can in time be removed from the ice. But in case of organic pollution, the reverse is true. Often the process of freezing may, and does, concentrate such impurities so that the ice *Wanklyn water analysis. 32 contains more in a given weight than the water from which it was frozen. This was shown by the experiments of the Committee of the National Board of Health. * * * * The difference in specific gravity of ice and water prob- ably is one reason for the excess of pollution in the ice. Such portions of the sewage as were lighter than the heav- ier water below the film of forming ice would naturally float to the surface and become entangled in the ice. From the results of some incomplete experiments of my own in this direction, it appears that the amount of dilution of the sewage, and the distance from the point of entrance, per- haps also the oxidation or partial destruction of the por- tions readily decomposed, are important factors in determ- ining the relative amounts of pollution in ice and water. In specimens taken from a badly polluted stream near the outlet of a large trunk sewer, there were several grains more (IF) of organic residue from the ice than from the water of the river, while half a mile below the ice con- tained almost exactly the same amount of organic residue as the water. Similar results were obtained from ice cut from a pond four miles below the city, which sewers into the river of which the pond is a part. The pond results from a dam across the river. The following experiment shows that the germs of bac- teria are not destroyed by freezing or boiling or both. After the sewage-polluted ice was melted, while evaporating to secure the solid residue, a condenser was attached and the water received in a Florence flask which had been care- fully washed in boiling water. The space between the tube of the condenser and the neck of the flask were filled with cotton wool to exclude the germs of the air as far as pos- sible. When the flask was half full it was placed over a lamp and the water boiled, the neck sealed with cotton wool until the water boiled freely. The flask, after the water had been thoroughly boiled, was sealed and placed in a warm room. In two weeks bright spots appeared, and soon the whole became turbid. Microscopic examination then showed the presence of bacteria and bacillus rods. Ice cut from the centre of a block which was cut from a sewage 33 polluted pond was placed in sterilized infusions by Prof. Pumpelly, and the flasks sealed. The development of bac- teria showed that the vitality of their germs had not been destroyed by freezing. There is another point to be considered in relation to the amount of contamination in ice from water polluted by sewage. As all chemical changes are less in winter under the influence of cold, the water from which the ice is formed would contain more sewage-pollution in a given bulk than in summer, because less would be removed by oxidation and still less by vegetation ; so that the ice would probably contain a greater percentage of sewage-pollution than the water in summer. The microscopic life is also very con- siderably lessened in winter, so that source of purification of the water would be removed. The cold would also have a tendency to prevent rapid changes in the sewage itself, so these products would accumulate. Thus it is readily seen that if such water is unfit to be used as a source of drinking- water in summer, it is unfit for a source of ice supply in winter. * * * * The effect of freezing upon the infective power of germs has not been fully determined, although their vitality remains. But even if infectious diseases cannot be con- veyed by impure ice the diarrheal diseases induced are suffi- cient to exclude the use of ice from such sources. The following case has been analyzed very closely, and the connection is apparently clear : There occurred a fatal case of typhoid fever in a gentleman who for some months had been living alone, his family being absent during the summer and early autumn. As the sanitary arrangements were as perfect as wealth and intelligence could make them, the cause of this isolated case was for a long time a mystery. The fact that he was inordinately fond of ice-water led to suspicion that might be the cause, and an investigation fol- lowed. The ice used was cut from a pond near the houses of laborers on his farm, and, unknown to him, the drains from these houses had been connected with this pond. During the summer there had been in these houses three cases of typhoid fever, one fatal, and the dejections had 34 been thrown into the water-closets without any disinfection. The common practice of the neighborhood was for the house-drains to empty upon the open ground whenever a lower level could be reached, or else in some convenient ditch, but as this pond was quite near the houses, the drains were led into it. As the pond had been used for fill- ing the ice-house for years, there was no suspicion of any contamination .until after the mischief had been caused- The connection seems clear. There is no doubt concerning the pollution of the water by the dejections from typhoid fever patients as reported, nor the other facts as stated. Had the ice been used by others to any extent, the proof would have been clearer. During the summer I examined thirty specimens of ice- water from ice as supplied to customers from the carts in five different cities. In none of those was there any evi- dence of sewage contamination, although one was suspici- ous, as the organic matters were largely in excess in com- parison with the others. The refusal of several companies to sell to the person that was sending specimens, after they had learned by chance the purpose for which it was wanted, led me to suspect that the source of supply was not above suspicion. The plan adopted was to have from twenty-five to fifty pounds of the ice melted and the sediment with about a quart of water sent. To secure uniformity, when the larger quantity had been melted, half the quantity sent was used. In twelve of the specimens there was no sediment deposited. In six there was not enough to cover the bottom of a quart bottle, and in the remaining twelve the deposit varied from an eighth to nearly half an inch. None developed offensive odors except in two instances, though the bottles were kept corked in a warm room four months, some of them. The bulk of the deposits was formed evidently of substances held in suspension in the water. The field and water grasses broken in small fragments were most frequently encountered. Next to these an amorphous floculent deposit or partly decayed vegetable matter that had been held in suspension in the water. The microscope revealed some 35 diatoms and fragments of microscopic plants and animals, such as are most common in fresh water, and some few specimens nearly complete. In one there were fragments of woody tissue, and several twigs and bits of bark; sand and dirt were not uncommon. In one there was an unusual quantity of specimens of various forms of microscopic plant and animal life, in connection with fragments of water, weeds, and grasses. There were but a few that developed any large amount of microscopic plant and ani- mal life; in fact, but two. In these there were extensive colonies of filamentous Algce developed. In but six was there any unusual amount of organic residue upon evapora- tion and incineration, and that apparently was of vegetable origin entirely. (Taken from the Fifth Annual Report of the Connecticut State Board of Health, for the fiscal year ending November 30, 1882. Pages 298, 299, first paragraph on page 300, page 301 and half of 302.) APPENDIX "D." Sickness From Impure Ice. In several instances the attention of the Board has been drawn to sewage-contaminated ponds with ice-houses on their borders, and strange to say, although the evidence of disease thus caused is so conclusive, many still adhere to the idea that water purifies itself in freezing. Even the process of melting the ice and demonstrating the presence of organic pollution by the microscope, will not always convince against the force of the pocket argument. Several isolated cases of enteric trouble, and one death from the free use of ice polluted by sewage, have been reported during the year. Fortunately an enlightened public sentiment is compelling 36 the abandonment of such sources of supply, customers insisting on knowing where the ice they buy is cut. (Taken from the Third Annual Report of the State Board of Health of the State of Connecticut, for the fiscal year ending November 30, 1880. Page 17.) APPENDIX "E." Impure Ice. Through the kindness of Dr. Orlando Brown, of Wash- ington, Litchfield county, under whose care many of the cases were, I am enabled to place on record the history of cases of disease resulting from impure ice. Through the agency of this Board, in several instances where large sup- plies of ice were cut from sewage-contaminated ponds or streams-indeed, so near to sources of contamination that it would seem no outside interference would be required- these sources have been abandoned, and purer supplies sought. No cases of disease were traced to the ice in the instances named, as the supply was so general throughout cities, but doubtless such cases did occur, and some of the apparently strange cases have been thus caused. However that may be, it is undesirable to use impure ice, and we are using all means to illustrate and enforce the fact that water is not purified by freezing when it contains any considerable amount of impurities. These cases are similar to the results published by Professors Wood and Sharpies, in the Massa- chusetts reports, and of Dr. A. H. Nichols, in regard to the impure ice at Rye Beach and the epidemic that followed its use, published in the seventh report of the Massachusetts Board of Health. (Taken from the Second Annual Report of the State Board of Health of the State of Connecticut, for the fiscal year ending November 31, 1879. Page 16.) 37 * * * Right here, and at the expense of some irreve- lance and of repetition, for the subject is of importance enough to bear repetition, it may be stated that freezing does not free water from organic impurities. The ice prob- ably contains less than the water, proportionately, but enough remains to produce disease and death oftentimes, where ice has been collected from sewage-polluted ponds. As we have repeatedly come in contact, in different parts of the State, with ice-houses along the banks of polluted ponds, sometimes where the water was exceedingly vile, recognizable by its odor easily in the summer months, and, as a corollary, occasionally sickness and death directly traceable to such ice, the fact is noted in this connection while considering dissolved impurities. * * * * (Taken from the Third Annual Report of the State Board of Health of the State of Connecticut, for the fiscal year ending November 30, 1880, third para- graph on page 12.) Ice and Snow Water In freezing, water becomes purer, losing a large portion of its saline contents. Even calcium carbonate and sulph- ate are partially got rid of. The air is at the same time expelled. Ice-water may thus be tolerably pure, but heavy and non-aerated. Snow-water contains the salts of rain- water with the exception of rather less ammonia. The amounts of carbonic acid and air are very small. There has long been an opinion that snow-water is unwholesome, but this, if it be true, is probably due to im- purities. Ice and snow often contain a good deal of sus- pended organic matter. Dr. Baker Edwards, of Montreal, found two grains per gallon in the shore ice and one grain per gallon in the river ice.* In Northern Europe, the poor classes have the habit of taking the snow lying about their dwellings, and as this is often highly impure with substan- ces thrown out from the house, this water may be unwhole- * Further evidence of the impurity to be sometimes met with in ice will be found in the Reports of the State Board of Health of Massachusetts, vols. vii and x. 38 some. It has been conjectured that the spread of the cholera in the Russian winter in 1832 was owing to the use of such snow-water contaminated by excretions. Ice and snow may also be the means of conveying malarious poison to places at a distance.* The Pittsfield Ice Supply. Nearly all the ice used here is cut on "Silver Lake," which is about twenty acres in extent, and situated to the eastward of the more thickly-settled portion of the town. Its outlet into the Housatonic River ordinarily dis- charges but little water, and the flow is sometimes even toward the lake, which is fed by springs and a small brook, which takes its rise in Frog Pond, about half a mile to the north. The sources of pollution on the brook are several dwellings and two factories, the one discharging a small amount of refuse dyestuff from silk-works, the other emptying the overflow of a cesspool for water-closets, used by one hundred and fifty employes. According to Dr. Adams, health officer of Pittsfield, the water is sometimes quite foul in appearance, but oftener comparatively clear. Looking upon this source of pollution with some suspicion, he sent. specimens of water for analysis. Prof. Nichols afterwards visited the locality, at the request of the Secre- tary of the Board, and obtained several more. From the results of analysis, it will be seen that the ice is consider- ably purer than the water of the pond, thereby showing that the process of freezing does tend, to a considerable degree, to purification. Chemical examination alone does not indicate that the ice is unfit for domestic use, nor does it ordinarily detect a very slight degree of pollution, which may be offensive to our sense, from the fact that we previ- ously knew of its presence. * See paper by C. Smart, M. B., C. M., Captain and Assistant Surgeon, United States Army, " On Mountain Fever and Malarious Water," American Journal of the Medical Sciences, January, 1878. See also Report on Hygiene, A. M. D. Reports, vol. xix. (Parke's Hygiene, page 21.) 39 Considering the great dilution of filth which may often take place without securing immunity from disease conse- quent upon taking such filth into the system, there can be no question as to the propriety of putting a stop to this con- tamination at once, especially in view of the fact that it is likely to increase rather than diminish. The large amount of inorganic matter is thought, by Prof. Nichols, to be due to mineral salts dissolved in the waters of the springs, which indeed have been found hard and unfit for use in boilers. There are a few dwellings, and a shoe-factory, on the borders of the lake itself. Examination of Water from Pittsfield, Mass. (Results Expressed in Parts per 100,000.) Number. Date. Locality. Ammonia. "Albuminoid Am- monia." Solid Residue. Chlorine. Inorganic. Organic and Volatile. Total at 212 degs. Fah- renheit. 74 Feb. 21, 1876 Frog Pond 1 0.0072 0.0212 2.64 2.44 5.08 0.04 75 Feb. 21,1876 Brook 2 0.0180 0.0120 18.08 2.28 20.36 0.36 67 Feb. 16,1876 Brook 3 0.0140 0.0180 9.58 2.78 12.36 0.21 58 Nov. 21,1875 Brook i 0.1400* 0.0348 16.64 4.64 21.28 0.67 68 Feb. 16, 1876 Brook 4 0.0060 0.0156 9.06 2.84 11.90 0.18 59 Nov. 21,1875 Lake 5 . 0.0210 0.0200 10.28 2.60 12.88 0.26 69 Feb. 16' 1876 Lake 5 0.0180 0.0152 10.32 2.72 13.04 0.30 70 Feb. 16,1876 Lake 6 0.0220 0.0140 11.92 2.60 14.52 0.35 71 Feb. 16,1876 Lake ? 0.0200 0.0136 11.60 2.68 14.28 0.38 Ice from f Unfiltered. 0.0072 0.0061 0.93 0.58 1.51 Lake, / Filtered.. . 0.0(177 0.0013 0.30 0.14 0.44 0.02 72 Feb. 16,1876 Housatonic River. ... 0.0042 0.0089 4.84 2.64 7.48 0.11 * Unfiltered gave ammonia, 0.1400 ; *• albuminoid ammonia." 0.0520. 1 Taken near the middle of the pond. 2 Taken at Lincoln street bridge, some distance above silk factory. 3 Taken just above drain from silk factory. 4 Taken between silk factory and lake. 5 Taken at eastern end of pond, midway between north and south shores,[at surface. 6 Taken at same point, near bottom, fifteen feet from surface. 1 Taken from bottom of pond at west end, thirteen feet from surface. (Taken from the Seventh Annual Report of the State Board of Massachusetts, January, 1876. Pages 274 and 275.) 40 APPENDIX "F." The Purification of Water by Freezing. By C. P. Pengra, M. D., of Ovid, Michigan. * * * * n0 one win contend that all ice is equally no more than that it is-absolutely pure, but will in all proba- bility admit that our aim should be to appropriate that the most pure and wholesome. With this end in view, at the instigation of Dr. Vaughan, we have been induced to make a series of experiments, by no means at an end, but the results of which to date have been as follows : Our first experiments were with crystalloids, and first of these with urea dissolved in distilled water and after- wards estimated by Leibig's method. 100 c. c.* of which before freezing contained .83 grams, f 100 c. c. of which from ice contained .50 grams. 100 c. c. of which not frozen, contained 1.3 grams. In other words a "casting out of .33 grams, or 40 per cent, by freezing.'' The second experiment with urea as found in normal urine, and estimated by nitrogen : 7300 c. c. before freezing = .91 of 1 per cent. 165 c. c. from ice = .42 of 1 per cent. Showing a purification of 53 per cent, by freezing. A third experiment with grape sugar in which 100 c. c. of ice contained 1.5 grams. 100 c. c. of ice contained .96 grams. A purification of 55 per cent, by freezing. In the fourth experiment arsenic (As 203) was dissolved in ammonia (NH 4 OH) and diluted to 1000 cubic centimet- * One c. c. = one cubic centimeter = .27 fluid drachm. I One gram 15.434 grains. 41 ers with distilled water, as analyzed by Mr. T. H. Hubbard, of the Pharmacy department. 1000 c. c. of original solution contained 1.78 grams arsenic (As208). 500 c. c. of ice solution contained .48 grams arsenic (As 203). 500 c. c. not frozen solution contained 1.3 grams arsenic (As203). A 40 per cent, purification by freezing. I may also add just here the one experiment by Hassall, in which a part of the water was frozen artificially. In the Original Solution. In the Ice. In the Water left. Total solids.... 27.0 3.0 14.2 Chlorine 1.94 0.9 - Lime 10.53 trace 14.11 The deduction from which would tend to prove more purification than in the former cases. Our next observation were of colloids, and we regret that they have been confined to albumens, but since they are quite constant as to results, the three will be better proof of the one fact. In the first, about 1,000 c. c. of a solution of egg albumen were frozen solid. 50 c. c. of the upper third contained 3.015 grams. 50 c. c. of the middle third contained 4.19 grams. While 50 c. c. of the lower third contained 6.87 grams. Showing a gradual but slight purification from above downwards. In the second experiment with albumen as found in albuminuria: 50 c. c. from ice contained .5 grams. 50 c. c. from unfrozen contained . 8 grams. Or a purification of about 20 per cent. In the third experiment with egg albumen: 50 c. c. of original solution contained .25 grams. 50 c. c. of ice solution contained .21 grams. 50 c. c. of solution left contained . 35 grams. A purification again of about 20 per cent. As will be noticed, the purification of crystalloids is at least 30 per cent, greater than that of colloids. 42 It is obvious that these results must vary with the rapidity of freezing ; but as these specimens were frozen naturally and under the varying temperatures to which common ice is subjected, they may be regarded as even more applicable to the general purposes of sanitary work. The conclusions drawn from the foregoing were quite beyond our expectations, not supposing that there would be so great purification as has been evident. But while this information has been somewhat of a surprise, it is also sufficient proof that we cannot, as sanitarians, admit the indiscriminate collection of ice, as is too often practiced, but should lead us to earnestly endeavor to persuade the public that pure ice can only come from pure water. That ice from water of cesspools or water receiving foul drainage, as from sewers, barns, privies, cemeteries, cellars, or containing any dead and decaying animal or vegetable matter, or from muddy streams or shallow and stagnant pools, cannot be pure, and the practice of gathering and using such is absolutely injurious to health, even if used for no other purpose than that so common of packing poultry or any fresh meats. In other experiments, which we hope to complete in the near future, we shall attempt to estimate the purification if any as affecting the numerous organisms so common in water. . * * * * . C. P. PENGRA. (Taken from the Tenth Annual Report of the Secretary of the State Board of Health of the State of Michigan, for the fiscal year ending September 30, 1882. Pages 49 to 50 inclusive.) APPENDIX "F" 2. * * * With reference to objections of companies taking ice from Fresh Pond, and to the extent to which any impurity in water is removed during the process of freezing,* * See also an article by Dr. A. H. Nichols, p. 455 of the Seventh Report of the Board, in which severe intestinal disorder was shown to have been caused by the use of impure ice. 43 Professor Sharpies made some experiments, the results of which may be found in the following table and remarks : Analysis of Samples of Water and Ice, by S. P. Sharples. (Results Given in Parts, per 100,000.) January 11, 1878. Ammonia. Solid Residue. Free. Alb. Total. Vola- tile. Fixed. Total. Fresh Pond water .0128 .0192 .0320 3.00 9.00 12.00 Fresh Pond ice .0060 .0075 .0130 1.50 3.50 5.00 Spy Pond water .0640 .0128 .0768 4.00 13.00 17.00 Spy Pond ice . 0064 .0064 .0128 1.50 3.50 5.00 Little Spy Pond water .0160 .0226 .0386 4.00 7.00 11.00 Little Spy Pond ice .0050 .0060 .0110 1.00 1.50 2.50 Pond-hole at slaughter house, near Fresh Pond, water 0.352 .1472 .1824 10.00 7.00 17.00 Pond-hole at slaughter house, near Fresh Pond, ice .0060 .0090 .0150 1.50 1.00 2.50 '' The depth of the water at the various places is about as follows : Fresh Pond 30 feet, Spy Pond 15 feet, Little Pond 20 feet, pond-hole 8 feet. The pond-hole w'as made by dig- ging out the muck in the swamp back of Niles Brothers' pig-house. ' ' The water from the melted ice was in all cases perfectly clear. The w'ater from the pond-hole, under the ice, was of a shiny wine-color. "The ice was broken into small pieces, and placed in a wide-mouthed bottle, which was tightly corked, and kept corked until the ice had melted. The water in each case was taken from the same spot as the ice. The ice was taken by clearing the surface, then chopping out a sufficient quantity with an axe, and afterwards breaking a hole through the ice, when the water was dipped out, and placed in a demijohn." These results of Professor Sharpies have been subse- quently confirmed by Professor E. S. Wood, who has also kindly given the following statement of impurities in ice examined by him for Dr. Amory, of Brookline. In the first case, the excessive amount of impurity may have been due to some local source upon the ice. A certain amount 44 of sickness was thought to have been traceable to it. Nos. 3 and 4 were directly from the pond : Nos. 1 and 2 were delivered to houses. Analysis of Samples of Impure Ice, by Prof. E. S. Wood. (Results Given in Parts, per 100,000.) Date. Locality. K) M K) td £ Clear. Inorganic Res. Organic and Volatile. Total Res. Remarks. Dec. 3, 1876 Horn Pond 0.0026 0.044 0.4 6 7.6 9.2 Very turbid. Jan. 23, 1877 Hammond's Pond 0.0066 0.019 1.0 1.4 2.4 Much clearer Feb. 12, 1877 Jamaica Pond Ice Company 0.018 0.016 0.3 0.4 0.4 0.8 than above. Quite clear. Feb. 12, 1877 Jamaica Pond Ice Company 0.026 0.016 0.3 0.4 0.8 1.2 Quite clear. (Taken from the Tenth Annual Report of the State Board of Health of Mas- sachusetts, January, 1879. Page 119 and half of 120.) APPENDIX "G." River Pollution. Early in February last the attention of the Board was called to the Pollution of the Ammonoosuc River, as fol- lows : Lisbon, N. H., February 2, 1882. Dr. I. E. Watson, Secretary State Board of Health, Concord, N. H.: Dear Sir-As Chairman of the Board of Health of this town, I desire to call the attention of the State Board of 45 Health to what I have regarded for quite a long time a public nuisance in this section. I refer to the filthy con- dition of the Amonoosuc River in consequence of filth and debris of all kinds being thrown into the river in towns north of us, but more particularly in the town of Littleton, where there is a large amount of glove-making done, and the hides are tanned there for that purpose. For a time, I was told it was their custom to cart this waste material away to the compost heap; but for several years, as I am informed, they have practiced throwing it all in the river ; and the amount of it is very large in the aggregate. I took the trouble several times during last summer to examine the water in the river, and found an abundance of hair n it every time. In the various processes of tanning with the chemicals used, you can hardly estimate the amount of filth which comes down the river. Manufacturers com- plain of its sticking to the racks of their flumes and ob- structing the flow of water, but I have never examined them myself. Some of our citizens are packing ice from the river in which hair is plainly seen frozen into it. Such ice, I think, is entirely unfit for use. Another source of pollution is in summer, when the water is usually low. The town or village of Bethlehem, and many of the large mountain houses, run their sewerage into the river, at least I am told such is the case. This, of course, would be a sensitive question for the Board of Health to approach, but I think it is one worthy of consideration, at least; for I think the time will come when those living near the river will demand that something shall be done. I make these suggestions for your consideration, and I feel that some action should at once be taken in regard to Littleton tanneries ; at least I hope you will take the trouble to investigate the subject, and satisfy yourselves. Respectfully yours, 0. H. BOYNTON. In my reply to the above, request was made that a sample of the contaminated ice be sent to the office of the Board for examination. Prompt compliance of the request fol- lowed. A box containing probably fifteen or twenty pounds of ice was received, and with it a letter, to wit: Dr. I. A. Watson: Dear Sir-Yours of the 28th came to hand last night. The samples of ice I sent you came from the canal near the mills, a few rods below the dam, while the ice for storage is cut in the mill-pond above the dam, some of it very near 46 the dam. I should have got it from there, but they had ceased cutting some time ago, and it was frozen over, and quite a depth of snow on it, and it would have been quite a job to get it. The people of Bath, who have formerly got ice from the river, found it so bad that many of them got their ice from Mink Pond, some three miles from here, making some eight or nine miles distance to draw it, as they are obliged to come through Lisbon to get to the pond. I should prefer that you come here and investigate for your- self, and you can easily get all the proof and affidavits or depositions you wish, and, I think, get a better idea than I can give you. * * * * Should you conclude to come, I will render you any assistance I am able to. Respectfully yours, O. H. BOYNTON. The sample of ice received, it will be seen, was taken from the river ten miles below the tanneries complained of, and Bath, to which reference is made, is fifteen miles below Littleton. The ice was sent Saturday afternoon, and by an oversight remained at the railroad station over Sunday. The warmth of the baggage-room, in which it was left, developed from the box such an intolerable stench that it was ordered to be placed outside of the building. The con- tents being unknown to the station men, it was a source of considerable apprehension and some alarm, as well it might be. The fact is important.only as an illustration that the ice must have been badly contaminated with putrefactive ani- mal matter to have yielded such disgusting evidence to the sense of smell. When delivered at this office, the odor was so offensive that the sample was placed in the yard outside of the building. A visual examination revealed large quantities of hair, small pieces of animal tissue, sawdust in a partially decomposed state, and fresh wood shavings, the latter of which was undoubtedly a product of the mills at the dam, below which the ice was taken. About two pounds of this ice was slowly melted in a warm room in a clean basin, and then put into a large bottle and thoroughly sealed. In a few days some small yellow-colored oil glob- ules appeared upon the surface of the water, which in the 47 course of a month entirely disappeared, leaving the water slightly tinged with yellow, in which condition it seems to remain without further change. The Tanneries. The glove business at Littleton is a comparatively new industry for that town, the business having developed in a very few years to gigantic proportions. The two large companies, the Saranac and Eureka, have each a tannery, aside from their large manufacturing establishments. Some idea of the magnitude of these industries may be gathered from the fact that the sales for the last year amounted to over $500,000 ; about 225,000 skins were used, weighing in the aggregate 700,000 pounds before being tanned. About three hundred men, women and children are employed at the tanneries and shops, and two or three thousand women, engaged a part of the time, in making gloves outside of the shop. The amount of waste estimated from the process of tanning, etc., is over two hundred tons, a greater portion of which has been allowed to go into the Ammonoosuc River. No direct complaint has been made to the proprie- tors of these tanneries, excepting from mill-owners below, and that on account of the obstruction caused by the hair which was carried down the river. Land-owners along the river were pleased with the result of the occasional overflow of the river, which carried valuable fertilizing material upon the soil, and resulted in large crops, and a more abundant hay crop, in some instances doubling the amount. A Visit to Libson and Littleton. April 6, the Secretary visited Lisbon for two objects : to examine into the cause of sudden fatality in a herd of cat- tle, elsewhere mentioned, and the contamination of the river. A few weeks prior to this time the ice had been broken up in the river by a sudden thaw and rise in the water, and immense masses had been piled up promiscuously in many 48 places between the two towns. Acres were covered in this way, and, riding along the railroad even, the ice presented a dirty and filthy appearance. Just above the village of Lisbon, and in view of the same, was a large field of ice, embracing an area of over fifty acres, compactly driven together, and in some places piled piece upon piece. Upon this the sun had shone three weeks or more, and, of course, it has been steadily melting a great part of the time, as the warm spring days approached. The ice of this great field contained, like the sample sent the Board in the winter, a large amount of hair, considerable animal matter, like fleshings, and sawdust in all stages of decomposition. The melting process had gone on so slowly that it did not wash this foreign matter off from the ice to the ground, but simply left it lying upon the surface of the ice. The whole surface of this field of ice was so discolored in this way as to present the extremely dirty condition which I had already described, while riding on the railroad, in the fields that were nearer the passing train. I had expected to find con- siderable pollution of this stream, but my mind had not been prepared to witness such wholesale contamination as was before me. An examination of the meadows on the opposite side of the river, where there was no ice, revealed the same condition that will exist when the vast quantities of ice mentioned are melted, to wit, an almost incompre- hensible amount of debris scattered over the 'entire surface. In some places this refuse material was evenly spread over the ground to the depth of an inch ; in other places it had seemed to drift, and was found to be in patches a few feet wide and several rods long, to the depth of from four to twelve inches ; in some other localities it had only discolored the surface without leaving it in any considerable quanti- ties, though everywhere could be found this particular waste, hair and fleshings from the tanneries. The immense amount of sawdust was another notable condition, and one which, though not so offensive in its character as the pro- duct of the tanneries, is by no means to be ignored. The bushes and shrubs which grew along the banks of the river were completely fringed with hair as far up as high-water 49 mark ; indeed, they furnished an excellent and accurate register of the height to which the water had reached. It was also stated that a turbine water-wheel at Lisbon, had become completely filled and packed with the hair, so that it was stopped in consequence of the same ; that the flume racks became filled by slow accumulation, and necessitated the removal of the same ; also that the paper-pulp mill at that place had to strain all of the water used in their vari- ous processes, and, in so doing, the pipes and strainers would become filled with hair, and oblige them to suspend work till the same was removed. These facts are men- tioned to give a faint idea of the extent of this particular trouble. The pollution of this stream is without a parallel in the State, owing to the character and extent of the contamina- tion. Information received at Littleton concerning the pro- cess of tanning, and the various chemicals used, leads to the conclusion that no substances were used in the various manipulations and methods employed in preparing the leather, which would at all injure the character of the river ; dilution and chemical changes through oxidation would be so rapidly that probable no trace of the substances used could be found even a few rods below their entrance into the river. The only contamination, then, coming from the tanneries mentioned, is of an animal character, to wit, hair and fleshings. From the nature of these substances, rapid decomposition and oxidation is impossible, conse- quently the pollution of the stream would be even greater at a given distance from the point of entrance into the river. How far these substances may be traced, below the Ammonoosuc, in the Connecticut River has not been ascer- tained, but undoubtedly to quite a distance. Ice taken from the Connecticut River, near the mouth of the Am- monoosuc, contained hair from these tanneries. Abatement of the Nuisance. After an examination of the condition of the river, and the immediate effects of such pollution, a visit was made to 50 the glove companies at Littleton for the purpose of settling, in some way, this difficult question. It was apparent that such a state of affairs could not be allowed to exist, as the result would be dangerous to the health of the people, detrimental to manufacturing interests, and in many other ways intolerable. Parties suffering from the results of such wholesale river contamination were upon the verge of commencing suits, not only for an abatement of the nuis- ance, but for special damages. This intention seemed in no way to be a malicious one, as it was stated, evidently upon good authority, that if the tanneries would cease at once putting their waste into the river, no suits would be entered Upon visiting Littleton, and interviewing the companies mentioned, they asserted that no complaint had been made directly to them, with a single exception, from any source, and, with a candor and promptness which are rarely exhibi- ted by prosperous corporations, especially in a matter which would result in cost and inconvenience, they expressed a willingness to immediately put in execution the recommen- dation of the Board, to wit, to allow no more waste from their factor les and tanneries to enter the river. This deter- mination, though resulting in considerable immediate expense, has been faithfully carried out, as a subsequent visit and examination have shown : so that this extensive source of pollution has been effectually removed. Too much credit cannot be given the Saranac Glove Company and the Eureka Glove Company (owners of all the tanner- ies) for the willingness and promptness which they exhibited in bringing the case to an early and satisfactory termina- tion, and that, too, in a generous and public-spirited man- ner. Without a question as to the legal view of the case, or a suggestion that they were within the reach of any of the various processes of the law, they responded simply upon being asked ; and the people who have been incon- venienced by the former pollution of this river should credit these companies with a spirit of fairness and consid- eration, which is indeed merited. It is to be hoped that all thoughts of suits in the case will, if they have not already, be abandoned. 51 It is to be expected that some of the results of this exten- sive pollution will be evident in some localities along the river during the present season. The debris which the early freshets in the spring carried upon the banks and meadows will of course remain, and, it is possible, may be a source of annoyance at some points, even at the present time ; however, the process of oxidation will very soon destroy all that may not be objectionable, and the original purity of the water and cleanliness of the banksand overflowed lands will, so far as the tanneries are concerned, be restored. The Contaminated Ice. That last winter's product of ice from this river between Littleton and the Connecticut River (twenty miles) is wholly unfit for use, does not admit of a doubt. Even ice taken by parties at Woodsville from the Connecticut River, near the mouth of the Ammonoosuc, contained hair in quite visible quantities. Under no circumstances should the ice mentioned be used for drinking purposes, even though to the eye it presents the appearance of absolute purity. It is a popular fallacy that ice purifies itself ; that water in freez- ing eliminates all organic impurities ; such is not true; w'ater that is contaminated produces ice of the same char- acter. There are many instances on record in which impure ice has produced serious and alarming sickness. It is not necessary that such ice should be contaminated to that extent that it may be seen with the naked eye, to render it unfit for use. Water may be badly polluted by foreign matters in solution, and the ice produced therefrom be equally dangerous to health. So, then, the present stock of ice from this river should be regarded with great sus- picion, however fine its appearance. (Taken from the First Annual Report of the State Board of Health of the State of New Hampshire, for the fiscal year ending April 30, 1882. Pages 53 to middle of 59.) 52 APPENDIX "H." Purification of Water by Freezing. By C. P. Pengra, M. D., Assistant in the Chemical Laboratory, University of Michigan. As a supplement to former experiments upon this subject (see pages 48-50 of the Report of Michigan State Board of Health, 1882,) I have made recent observations, which con- firm the conclusions then drawn, that freezing does not purify water to the extent generally supposed. I then found that the purification by freezing was about fifty per cent, for crystalloids, and only about twenty per cent, for colloids. As the results presented in my former paper were not concerned with the living organisms so frequent in polluted water, the following experiments were deemed necessary. The micro-organisms used were such as may be obtained from decomposing meat juices, infusion of hay, and stag- nant pools. Each specimen of the fluid was divided into two equal portions, one to be frozen, and the other to be reserved for comparison. The freezing was effected by exposing the fluid to the winter's cold. In each experiment ten drops of each portion were examined and the averages taken. The first experiment was with bacteria. The average number per drop found in each portion was : In the melted ice of the upper half of the exposed Fluid, 16. In the lower half, partially frozen, 250. In the upper half of the unfrozen portions, 160. In the lower half of same, 170. In all cases the number of organisms in each of ten drops was counted and an average taken. This experiment shows 53 a purification of about 90 per cent. The averages in differ- ent portions of the unfrozen fluid show that the organisms were quite evenly distributed therein, which was also true of the following. The second experiment was with the coleps hirtus of stagnant pools. The averages being taken as before, the following results were obtained : Upper third of ice contained 25 per drop of water. Middle third contained 190 per drop. Lower third contained 500 per drop. In the unfrozen fluid the average of fifteen drops was 300. This shows a purification of 91.6 per cent, in the upper third of the ice, with a gradual decrease downward. In this case the fluid was frozen through and through solid. In a third test with paramoecium aurelia from a muddy pool the following results were secured : The water from the ice contained 30 per drop. The water under the ice contained 590 per drop. The unfrozen fluid reserved averaged 450 per drop. This showed a casting out of 93.3 per cent. The fourth test was with glaucoma scintillans from infusion of hay ; the following figures were obtained : Water from upper half of ice contained 10 per drop. Water from lower half of ice contained 120 per drop. Unfrozen liquid averaged 91 per drop. A purification of 89 per cent, is secured in this case. These figures seem, at first, quite encouraging, especially when compared with the results given in my former paper with chemical impurities. However, when we remember that freezing does not kill these organisms, and that they multiply rapidly, there is not so much consolation, as such ice may prove a fertile source of infection. The entire series of experiments shows that pure ice can come only from pure water, and that great care should be exercised in gathering this useful commodity. Admitting the above, the question as to the best time of gathering ice arises. In answering this, the following 54 analyses may be of value, not only as showing how impure ice may become ; but the circumstances attending its for- mation, together with its natural surroundings, have a direct bearing on hygiene. The ice, which was about 18 inches thick, was cut and examined in such a way that we may speak of its upper and lower halves. One liter of water from the upper half gave the following results : Total solids, 0.5 grams. Free ammonia, 1,333 milligrams. Albuminoid ammonia, 0.8 milligrams. Nitrates, abundant. Analysis of one liter of water from the latter half showed the following: Total solids, 0.1 grams. Free ammonia, 0.12 milligrams. Albuminoid ammonia, 0.16 milligrams. Nitrates, test. Taken alone this analysis seems to disprove the fact that there is a gradual purification in freezing, downward ; but a brief consideration of the circumstances will solve the mystery. The stagnant pool (for such it was from which this ice was taken) is surrounded by steep hills, which begin at the very water's edge. At the summit of the hill on one side, stand four houses, each with barn and privy. On the opposite side of the pond the hill-side is covered by an old cemetery and has a considerable growth of trees. The lower half of the ice was frozen early in the winter, and the water frozen at that time underwent the partial purifi- cation shown to take place in our experiments. Subse- quently there was a fall of snow, followed by a thaw and rain. The decomposing matter from the hill-sides was washed down upon the first layer of ice, and, it becoming colder again, there froze, a distinct color in the ice marking the depth of this increase. Whatever purification might have taken place was limited by the surface of the lower half of the ice, as a result of which we have no purifica- tion in the upper half. We can easily see that in this way many additional layers of impure ice may be added. 55 From the foregoing we must conclude that the pollution of ice often accompanying partial thaws and subsequent freezings should be considered in harvesting, and that the first ice of winter is the purest. It is needless to suggest that skaters carry large amounts of impurities on the ice, .and that fields selected for harvest should be protected from them. Even a heavy fall of snow upon ice may contami- nate it to a considerable extent. An analysis of snow fall- ing upon a pond near the laboratory, collected during the storm, showed the following : Total solids per litre of water from the snow, 0.12 grams. Free ammonia per litre of water from the snow, 0.4 milligrams. Albuminoid ammonia per litre of water from the snow, 0.21 milligrams. The excess of free and combined ammonia may be in a measure accounted for by the nearness to the chemical laboratory of the University of Michigan. The purest snow, gathered away from cities, contains on ah average; Free ammonia, 0.3 milligrams per litre. Albuminoid ammonia, 0.08 milligrams per litre. Since writing the above I have been favored with an opportunity of testing still further the validity of the ground taken. A specimen of ice, which has been garnered before any rain or thaw has come upon it, showed on analysis the following results, per litre : Total solids, 0.08 grams. Free ammonia, 0.19 milligrams. Albuminoid ammonia, 0.09 milligrams. Nitrates, none. I afterwards gathered some ice from the same source, but there had been some rain, snow, and drainage from thaw- 56 ing upon it. Skaters also had been upon the ice. Analysis showed the following results, per litre : Total solids, 0.6 grams. Free ammonia, 0.28 milligrams. Albuminoid ammonia, 0.6 milligrams. Nitrates, very abundant. The deductions from these analysis serve to confirm my former convictions, and sustain the conclusions already given. C. P. PENGRA. (Taken from the Twelfth Annual Report of the Secretary of the State Board of Health of the State of Michigan, for the fiscal year ending September 30, 1884. Pages 79 to middle of 81.) APPENDIX "H," 2. Proceedings of Academy of Natural Sciences, Philadelphia, Oct. 28, 1884. Organisms in Ice-Prof. Leidy stated that a member had placed in his hands, for examination, a vial of water obtained from melting ice which is used for cooling drink- ing-water. From time to time, among some sediment taken from a water-cooler, the gentleman had observed what he supposed to be living worms, which he suspected were introduced with the water into the cooler, and not with the ice. Upon melting some of the ice alone, the worms were still observed, and the water submitted for examination was some that was thus obtained. Prof. Leidy was surprised to find a number of worms among some floculent sediment, mainly consisting of vegetal hairs 57 and other debris. Beside the worms, there were also imma- ture Anguillulas, and a number of Rotifer vulgaris, all living. It would appear that these animals had all been contained in the ice, and had been liberated on melting. It was an unexpected source of contamination of our drink- ing-water, that Prof. Leidy had previously supposed to be very improbable. The little worms he was not familiar with. (Academy of Natural Sciences-Proceedings, 1884. Page 260.) APPENDIX "H," 3. The Action of Cold on Microphytes.* Professor McKendrick, of Glasgow, gave at the recent meeting of the British association an interesting account of the methods of trying to destroy small organisms like bacteria, not as is commonly done by heat, but by cold. It is known that by means of Coleman's cooling machine meat may be kept from putrefying for a considerable time, but in attempting to sterilize a putrescible solution by means of cold, it was found that, though in some cases putrescence was delayed, in no case were the organisms completely destroyed. Organic fluids were exposed to temperatures more than 120 deg. below 0 deg. F., but on thawing they were found to contain living organisms still. * * * It is well known that frogs have been found in blocks of ice and been revived. Frogs have been frozen at 20 deg. F. in about half an hour. On thawing slowly, the animal, in I *From Nature. 58 two instances, completely recovered. When it was frozen for longer than half an hour it did not recover ; but, though reflex action was gone, there remained some irrita- bility both in nerves and muscles. It was found also that certain vital functions may be arrested by cold, and thus conceivably higher organisms may be kept vitally inert for an indefinite time. Experiments were also tried on warm- blooded animals. A rabbit subjected to a temperature 100 deg. below 0 deg. F. recovered. No temperature lower than 73 deg. below 0 deg. F. has been obtained in free atmosphere. (Science, October 30, 1885. Page 393,) APPENDIX "I." Syracuse, April 3d, 1886. To James T. Gardiner, Esq., State Board of Health : I, Wm. H. Pollman, Health Inspector to the Board of Health of the City of Syracuse, do hereby testify that the facts given below are as near correct as it can be given. By a resolution passed by the Board of Health, February 10th, 1886, the Health Inspector was instructed to get ice and water from the following places, Onondaga, Cazenovia Lakes and Wide Waters. The ice and water were taken from lakes and wide waters on March 3d, and sent to Albany the same day. Mr. Sawmiller's ice and water were taken from Onondaga Lake 3,000 feet from the mouth of Onondaga Creek, and 1,200 feet from the shore and from mouth of Bear Trap Creek. Gee Bros', ice and water were taken from Onondaga Lake 1,500 feet from the mouth of Onondaga Creek, 1,200 feet from east shore of lake. 59 Listman's, now Warner's, ice and water, marked No. 2, were taken from Onondaga Lake two miles from the mouth of Onondaga Creek and 600 feet from east shore of lake. Knapp's ice and water were taken from the Wide Waters at the foot of Cherry street, about 100 feet from south dock, near or south side of the channel. Listman's ice and water marked 5, were taken from Cazenovia Lake in center and 300 feet from east shore in thirty feet of water. Respectfully, WM. H. POLLMAN, Health Inspector, Syracuse, N. Y. Sworn to before me this, the fifth day of April, 1886. P. D. COONEY, Commissioner of Deeds, Syracuse, N. Y. APPENDIX "J." Laboratory of the Albany Medical College, ) Albany, March 20th, 1886. \ Dr. F. C. Curtis, Acting Secretary, State Board of Health, Albany: Dear Sir-I transmit herewith a tabulated report of the analysis of five samples of water and five of ice, which samples were sent to the State Board of Health, by Wm. H. Pollman, Health Officer, of Syracuse, N. Y., and delivered to me on March 4th, with the exception of sample of water No. 5, which was received March 9th. The water was contained in large glass bottles and the ice consisted of large cakes, with labels securely attached, packed in saw- dust. The ice was unpacked as soon as received, and each cake separately cleansed, broken in pieces, the pieces rinsed 60 in distilled water and placed in large glass jars closed air tight and allowed to melt. The "color and appearance" and " odor" as stated in the table of results of course refer to the water from the ice and not to the ice itself. By request of Mr. Carman, samples of each of the waters and ice were sent to Dr Wm. Hailes, for biological examina- tion. It will be observed that the three samples of water from Onondaga Lake and the single sample from Wide Waters, Erie Canal, are rated as "bad" while that from Cazenovia Lake is classed as "doubtful." The chlorine in the Onon- daga Lake waters is exceedingly high, but this may be partly or chiefly owing to the geological strata in the vicinity. The samples of ice with the exception of No. 2 from Onondaga Lake are rated as ''good." In No. 2 the albuminoid ammonia is low but the free ammonia rather high, and it is classed as "not completely satisfactory." It will be observed that freezing has almost entirely removed the dissolved solids which the waters contained, including the chlorides, and has reduced the amounts of free and albuminoid ammonia very greatly, and largely diminished the amounts of oxygen absorbed. From a chemical stand- point the ice is vastly purer than the waters, but this does not necessarily prove that micro-organisms or specific germs if present have been destroyed or removed in a similar ratio. Yours respectfully, WILLIS G. TUCKER, Analyst.