GENERAL HEADQUARTERS UNITED STATES ARM! FORCES, PACIFIC OFFICE OF THE CHIEF SURGEON (Advance Echelon) 5 December 45 TO: The Chief Surgeon, GHQ, AFLAC, APO 500, Manila THRU: The Chief Surgeon, Adv, Ech., Tokyo INFO: The Surgeon General, Washington, D.C. SUBJECT: Final report of The Committee for the Technical and Scientific Investigation of Japanese Activities in Medical Sciences, 1, This committee was established at Manila by Order No, 640 of the Chief Surgeon;s Office, GHQ, AEPAC, APO 500, dated 22 August 45, with the orpeotation of benefiting from independent Japanese research and development in the field of medicine and thus gaining in the overall knowledge in this science. The dele- gated functions of the committee were: a. Examine and evaluate such Japanese installations and personalities associated with the medical sciences as may be practical during the ocoupo.tion. b. Correlate the activities of the medical section of the Enemy Equipment Intelligence Units attached to the armies, c. Engage in the investigation of such other phases of Jap- anese Medical activities as may be directed by the Chief Surgeon, 2, The Committee arrived in Japan in two sections. One came with an Eighth Army unit (42nd General Hospital) to Yokohama, arriving at the same time as the advanced echelon of GHQ, and the other approximately a month later, with the Sixth Army (135tii Medical Group) to Wakayama, then to Tokyo, The committee operated as a section of the Chief Surgeon*s Office, Adv. Ech,, Tokyo, 3, The plan for carrying out the investigation, as set up at Manila, was changed through force of circumstances and through prescribed procedure developing from the peaceful occupation and general cooperation from Japanese officials. The method of in- vestigation that developed was, in general, as follows: Activities of the committee followed command channels, keeping in touch with the surgeons of the various echelons. Hull cooper- ation was received from each. Official .liaison channels were utilised in dealing with the Japanese, In many cases the Japanese Central Liaison Office, on request through G-2 Japanese Liaison,. made available a representative to facilitated arrangements^V"'J 4# In the above manner, over the past 3 months, every city of in medical education and every first class medical institution associated with research and developments in Japan, was visited. The heads of the institutions and professors of their departments were interrogated. Reprints and manuscripts were secured to supplement information gained during the interro- gation, It is believed that a complete screening of Japanese Medicine for new deve1opments and methods was accomp 1ished 5. A vast amount of literature and information concerning all sorts of medical activity over the past 20 years became sub- ject to examination. This forced the committee to become a-' • * "screening" rather than an "investigating51 agency. During the screening procedure, new developments, original research since 1940, unfamiliar procedures and theories evolved, and literature produced since 1940, were sought, 6. Through neccssity. imposed by the scope of the mission, the committee accepted statements and reports from persons inter- rogated which it was unable to examine and properly evaluate. Many claims, some of them of definite scientific interest, were made by the Japanese, These claims, though not accepted as factual lave been reported, in the periodic reports. They were considered of interest and reported with that in view, ?• During the investigation other agencies, with somewhat similar interests, began their activities. Those activities were coordinated with those of the committee as far as was possible. Information secured by the committee was made available to other agencies, when it seemed to be of primary interest of the other agency and to be reported upon by that agency. Thus, matters pertaining to Aviation Medicine were turned over to ATIG, ISAP; those pertaining to BM,to theBW off ioe$ ,APPAG; those of naval interest, to.NNavTechJap, SOAP, including research projects report from the Naval Medical College; those concerning the Atomic Bomb Atomic Bomb Survey; Technical Intelligence to G-2, AFPAC; and the Chairman acted as medical member of the Scientific Intel- ligence Survey, B, Staff supervision of Medical Technical Intelligence was exercised through G-2, APPAC. Plans, for the completion of tech- nical intelligence coverage of Japan, were implemented, v 9. The screening of Japanese Medical Activities in the Homo Islands, with the exception of certain "Class B" institutions, which are considered of little consequence, is now completed, and such has been reported upon, 10, Attached, as Appendix "A51, is a complete list of reports on institutions and special subjects. Attached, as Appendix "B", is a list of individuals whose activities have been exainined. As Appendix "Ci}, is a list of items of interest, with reference to sections’of the report, and Appendix "D" is a Mfet of institutions screened. 11, Composition of the Committee was as follows; Lt, Col. William S. Moore, M.C, Lt, Col, James A, French, M.C, Lt, Col. Dan Tucker, M.C, Maj, Ray E, Trussell, M.C, Maj, Theodore G, Anderson, SnC, Maj, Arthur Stull, SnC, Maj, Charles L, Looker, M.A.C* Capt. John E, Tohie, SnC. Capt, Edgar J. LaLonde, M.A.C, W.S. MOORE Lt, Col„, Medical Corps Chairman Index "A” - Reports listed under Institutions, Index "B" - Persons whose activities have been examined, listed alphabetically and by geographical areas. Index "C" - List of Items of Interest Index ,JD" - Alphabetical list of Institutions screened. Distribution; Chief Surgeon, GHQ,, AEPAC, Manila#* 3 copies Surgeon General, Washington, D,C5 info copies A C of S, G-2 , GHQ, AEPAC ...7 ,f Economic & Scientific Section, SCAP...1 " ” Public Health & Welfare, SCAP*••••••••3 " !l NavTeohlap - Comdr, Ayres* .2 " ATIG,EEAE- Capt. Castor••*•**•*.•«*.1 ” tt U.S.S,B*S., Medical Section 1 " 11 APPENDIX ,fAn Reports listed under Institutions. I TOKYO B1PERIAL TJNIVERSITY Government Institute for Infectious Disease Manufacturing Division Parasitology Dept, Colloidal Preparations of Various Metals and Biologiccls B. Encephalitis Vaccine B. Encephalitis Epidenology Dengue Ac tit e Liver Necrosis cam to Infection of SneJ.lfish The Action of Immune Serum on the Japanese Encephalitis Virus cultured in Vitro Infectious Hepatitis virus isolation and cultivation Dysentery of Unknown Etiology occurring on Shikoku II, TOKYO IMPERIAL MEDICAL COLLEGE Institute of Pharmacology and Pharmacy Pathology Department, Serology Section Brain Research Institute Opthalmology Dept, Division of Physiotherapy and Internal Medicine HI. ARMY MEDICAL COLLEGE, Tokyo Clinical Division Biologic Manufacturing Division \ Parasitology Section Physical and Chemical Laboratory Appendix "A" III. ARMY MEDICAL COLLEGE (continued) Penicillin Research Committee Manufacturing Division, Niigata Dept, of Pharmacology Kanazawa Branch Field Manuals Research Projects and Findings Organization of Japanese Army Medical Dept, IV. ICEIO UNIVEPlSITY MEDICAL. COLLEGE Chemistry Dept, Department of Parasitology Anatomy Department Journal of the Faculty V. APu.LT EXPERIMENTAL STATION, Okuba Medical Section General VI. KITASATO INSTITUTE FOR MTFEGTIQUS DISEASE, Tokyo VII. INSTITUTE OF PHYSICAL AND CHEMICAL RESEARCH VIII. EYGIENIC LABORATORY. Tokyo IX. NAVAL MEDICAL COLLEGE Organization Preventive Medicine Department of pharmacy X. TECHNICAL INTELLIGENCE Appendix "A" xi institute 0? PUBLIC HEALTH, Tokyo Outline of Institute "Myopic Problem" Chemotherapeutic Institute, Ichikawa, Chiba XII NAGAO INSTITUTE XIII CHINA G-OVERMMENT MEDICAL COLLEGE XIV NIIGATA GOVERNliENT MEDICAL COLLEGE Cepharanthin Pathology and (Parasitology) Dept. Pathology Dept, Dept, of Biochemistry Clinical Division XV, TOHOICU IMPERIAL DIVERSITY MEDICAL COLLEGE, Sendai Dept, of Pathology i Parasitology Dept. * Bacteriology S. Ota Cf*'v>ic S Institute of Tuberculosis and Leprosy Institute of Tuberculosis and Leprosy (Investigation relating to Diabetes) Clinical Division S Medico-Chemical Institute Pharmacology XVI HOICHAIDO IMPERIAL UNIVERSITY. JUDICAL COLLEGE. Sapporo Bacteriology Appendix "A,f XVI, HOKKAIDO IMPERIAL UNIVERSITY, MEDICAL COLLEGE (conti nued) Pharmacological Laboratory Nakamura, Bacteriology and Immunology Hygienic Laboratory Clinical Division Hot Springs Research Institute Pathology Department Biochemistry XVII. HOKKAIDO IMPERIAL UNIVERSITY Veterinary Faculty Dept, ofAgricultural Chemistry Dept, of Agricultural Chemistry, Applied Mycolocy Cryological Institute XVIII, MINISTRY OF HEALTH AND SOCIAL AFFAIRS, TOKYO Bureau of Hygiene and Sanitation XIX. NATIONAL RESEARCH COUNCIL OF JAPAN Projects Reports on Projects XX. KANAZAWA GOVERNmiT MEDICAL COLLEGE Biochemistry Department Intenal Medicine Dr, T, Tani, Director of Bacteriological Institute Department of Pharmacy Department of Pharmacology Pharmacology (Dr, H. Okamoto) Appendix "A" XXI. KYOTO IMPERIAL UNIVERSITY. HELICAL COLLEGE Leprosy Institute Microbiology (Dr, R. Kimura) Pharmaceut ic al D epartmont Department of Pharmacology Research Projects Tropical Diseases Utizino-Laboratorium for Chemical Research XXII .KYUSHU IMPERIAL UIXlERSITY Bacteriology Dept* Hygiene Dept. Orthopedic Surgery Pharmacology Pathology Dept, Public Health Dept, Clinical Division XII11 .NAGOYA IMPERIAL UNIVERSITY. Faculty of Medicine Anatomy Department Department of Bacteriology Clinical Division Comments on Medical Education Hygiene Department Opthalmology . • Pathology and Parasitology Dept, of Pediatrics . Pharmacology (Miwa) Appendix 11 A" XXIV. OKAYAMA UNIVERSITY. Faculty of Medicine Anat omy D opartment Bacteriology Department Hygi en e Do partment Internal Medicine Pharma oology D opartment Pathology Physiology Biochemistry XXV. OSAKA IMPERIAL UNIVERSITY Department of Biochemistry I Microbiological Institute 1 Department of Pharmacology Physiology Laboratory Surgical Clinic Taboo Institute for Tuberculosis Internal Medicine XXVI, TOKYO JIKEIKAI MEDICAL UNIVERSITY Dept, of Forensic Medicine XXVII* AGRICULTURAL EXPERIMENTAL STATION Director of Research XXVIII. JAPANESE ARMY DEPOT FOR MEDICAL MATERIAL Rc scarch Department XXEC. RESEARCH INSTITUTE OF TUBERCULOSIS Dr, H* Oka, Director 50QC, WARTIME RESEARCH COMMISSION Board of Technical Science XXX!, EDUCATIONAL FOR EPICAL DEGREE - Igalmsi Alphabetical list of persons whose activities hfeVe been examined: NIIGATA AREA jXIV GOVERNIvEENT MEDICAL COLLEGE - NIIGATA Name Associated with-; AKA2AKE, K, Pathology AMANO ARIYAMA, N, Biochemistry HASHIHOTOj Takashi Dermatology - Dean of school ITO, TV * Bacteriology ICAITO, R, Bacteriology and Serology KEHARA, K. Pharmacclogy NAKA5JA, M, Surgery NOSAIvIO, S. Radiology SHIBATA, I, Internal Medicine TASAICA Internal Medicine YASUDA, M, Bio chemistry SENDAI AREA XV TOHOKU IMPERIAL UNIVERSITY, MEDICAL-COLLEGE EBINA, T, Bacteriology KUMAGAI, Taizo Director of T,B, and Leprosy Institute. President of Tohoku University, KATURE, A, Neurosurgery KATO, Toyojiro Emeritus Prof, of Medicine KOGA, Y, Radiology MASAMDNE, H. Biochemistry MATSUOKA, S, Neuro-Pathology MIKE, I, Orthopedics MDTO, M, Surgery NASU, S. Pathology OSATO, S, General Research (formerly at Kanazawa) SATO, S. Bacteriology TERASAKA, M, Pharmacology YATSUYANAGI, S, Parasitology aAPP-OaO AREA XVI HOKKAIDO IMPERIAL UNIVERSITY, MEDICAL COLLEGE AMBO, H. Pathology ARIMA, II* Medicine INOTJYE, Zenjuro Hygiene ISHIBASHI, Toshima Psychiatry P/ASHITA, K, Urology and Dermatology KODAMA, S. Anatomy - Dean of school KON, I, Pathology - Pres, of school MASAKI, Takio Pharmacology MIEAMI, J, Surgery HgApBDRO AREA (continued) ’XVI HOKKAIDO IMPERIAL DIVERSITY, MEDICAL COLLEGE Associated with: MINOSIMA, T, Physiology NAGrI, .11, Pediatry NAICAGAWA, S. Medicine NAKAMURA, Tutakfer Bacuoriology 0N0, S. Gynecology OCHI, Sad ami Emeritus Prof, of Opthalmology SAETO, 8, Director, Hot Springs Re- search Institute, SASAKI, Y. Mycologist SAWATARI, J. Otology SUGINOME, H. Organic Chemistry TAKEDA, K. Pathology UYENO, S, Legal Medicine YAMASAKI 5 H. Anat omy YANAGI, S. Surgery YASUDA, M. Biochemistry XVII HOKKAIDO IMPERIAL DNIVHRSITY HAIvIADA, S. Bacteriology in Vet. Medicine KANZAWA, M. Applied geology ISHIKAWA, K. Veterinary Medicine - Dean ITO, Nuho Ass*t. Prof, of Agricultural Chemistry. KOHANAKA, C, Hematology in Vet. Medicine KUROSAWA, K. Chstetrics in Vet, Medicine NAKAMURA, N. Medical Director, Cryologioal Institute, TAiCAHASHI, E« Prof, of Agricultural Chem. KANAZAWA AREA xx government? medical college, kanazawa AKIMOTO, H, Psychiatry HEKI, H. Internal Medicine HIRAIVATSU, H. Physical Therapy INOUE, T. Forensic Medicine ISHI1CAWA, T. Pathology ISHIMARU, S. Anatomy I SHI Z AKA, N. Dean of school IWAS/Ha, Ken Biochemistry IZUlviI, S. Podiatry KISAMORI, S, Gynecology KUMANQMIDO, S, Surgery KUROCHI, Y. Opthalmology KURU, M. Surgery MATSUDA, K. Otolaryngology MIYATA, S. Pathology NAMIKI, I. Dermatology OTANI, S. Hygiene KANAZAWA AREA (continued) fa GOVERNMENT MEDICAL COLLEGE, KANAZAWA Name Associated with: 'OKAMOTO, H. Institute of T.B. SAGUCH1, S. Anatomy fcAICURAI, Yosio Pharmacy TANI, T. Bacteriology TAILING, F. Internal Medicine UENO, K. Physiology KYOTO AREA XXI KYOTO IMPERIAL IMIVERSITY, MEDICAL COLLEGE AKACHI ' Biochemistry AMONG, S. Pathology ARAKI, Neuro surgery FUJINAMI, A* Pathology FDKITANI, A• Pathology fUNAOKA, Anatomy HATTOR, S. Pediatry INODE, G. Pathology KEvIURA, R. Microbiology, Dean of College MATSUMOTO, T* Pathology OGASAWARA, Noboro Director, Leprosy Institute and Microbiological Institute. OGUI, Kilcuo Pharmacy SLICE, Talceka Pharmacology SHBIANOTO, M. Pathology TAICAHASI, Torizo Biochemistry TODA Physiology DNNO, G. Pathology DTZINO, S. Director Utzino Laboratory - Chemistry YAMAZOICI, H. Pharmacology YAMOR,T. Pathology YOIvLAGUCHI, S. Science, Parasitology KYUSHU AREA XXII K30JSEU IMPERIAL UNIVERSITY m • ” Rioml.-i * Hygiene and.vParasitology HIHOEATA, R. Biochemistry Tokushi * Pharmacology I.JCi, H. - ’’ .> Dpthalmol'ogy IMAl, Tmkaiai -- thology ilTOuYE, 'K. (K# Ishikav/a) Radiology * *•* > - - ‘ ISMIYAIA, F k Surgery liTRmaiCA, S. Orthopedics - Dean of school 1CUSANAXI, G. -'O Medicine *m,.rh toSUYA, T. Urology MITUSIHA, Harvo Public health NAKASEIMA. T. Radiology FBS»0«!ft AREA (continued) XXII KYUSHU BIPERIAL UNIVERSITY ' ', Name Associated with: OGATA, D. Physiology SASAKI, M. S# E, N. T* TANURA, S , 0pthalmology TODA, Taclao Bacteriology and Protozology UMAYAIiABA, S. Obstetrics and Gynecology NAGOYA AREA XXIII NAGOYA IMPERIAL UNIVERSITY, Faculty of Medicine AiCUNE, M. E* E, Nc T, HAGINO, R. Opthalmology HOTTA, K, Biochemistry KATSANUMA, A. Internal Medicine - Director of Hospital KIIGCAWA, U, Obstetrics and Gynecology ICIRAIIARA, S. Surgery KOINDIA, Bogo Hygiene KONO, I. Physiology MX.TA, M, Pharmacology KAGAMATSU, H. Anatomy NANAS, Kyoichi Pedi&try NAlCASHBIA, M. Opthalmology OGASAWARA,. Kazuo Bacteriology OKADA, S. Internal Medicine OSEBA, Fukuzo * Pathology and Parasitology SAITO, W. Surgery SUGITA, U. Psychiatry TA.IURA, H, • Dermatology - Dean of school TOGARI, C . Histology UAGURA, S. Orthopedics YAMADA, K. Histology E* E, No T. OKAYAMA AREA XXIV OKAYAMA UNIVERSITY, Faculty of Medicine HAMAMA.TO, E. Pediatry IIATA, B. Opthalmology KAYASHI, Kanae Physiology HAYASHX, M. Psychiatry HAYASHI, S, Director of Research, Haya&hi Pharmaoeutical Go,, Ltd, INADA, S, Medic ine iCITAYAMA, K. Medicine MURAIGDvlI, Sakae Bacteriology NEGISHI, H. Urology and Dermatology OGATA, Masuo Hygiene OEUSHBA, K. Pharmacology OKAYALA AREA ' '('continued) XXIV OKAYAMA. UNIVERSITY, Faculty of Medicine Name Associated with; SEKI, Masaji PresJapanese Anatomy Society TAEI, Shimizu Biochemistry TAICEDA, T, X-Ray TANABS, Hiroshi Pathology TARO, ICazimo Biochemistry TSUDA, So Surgery YAGI, Hideo Gynecology and Obstetrics OSAXA AREA :CKV OSAKA. IMPERIAL UNIVERSITY, MEDICAL COLLEGE FUGHUSHIMA. K. Internal Medicine FUSE, N. * Internal Medicine IMAMURA, Arao Director, Takeo Institute for Tuberculosis ICHIIIARA, Bioohemi stry KAGIWARA, S. Hygiene ICU30 Biochemistry KINOSITA Director, Cancer Institute - Pathology RUROTSU, T. Anatomy MABUCHI, H • Phy s i o 1 o gy NICHIZAWA, Y. Pediatry OMURA, T• Forensic Medicine OKAGAWA, M» Pharmacy OTAHI, S# Director, Bacterial Chemistry Institute. SATAN I, Y* Director, Institute of Leprosy TAMB.lURA, T « Dermatology 1VJCABAYASHI, H. Surgery TAICAGI, H. Anatomy - Dean of Med. School TANIGUCHI, Tenjft Bacteriology - Director, Microb io1ogical Institute YAMAEAWA, K. Otolarynology Y0SHIMAT SU, N, Gynecology and Obstetrics YOUHIDA, S, Parasitologist (Retired) TOKYO AREA Heme H Associated with; Institution iilDO, K, Hygienes XIII AKAMATSO, S. Biochemistry XIII jMCTYA, Satiro Toxicology II ARIFUXA, Sheichi Chemo-therapeutios XI ASANO, Mitizo Biochemistry; Reg. Member I ASHIDA, Taizo XVIII AZUMA, Ryotaro Pharmacology II lilASSl&MA. Shuji Biochemistry; Reg, Member I, XI |ATSUTA, Y. As sit. Chemist XII IjlAYASEI, H. Director II IIAYASEI, Ryozo Lt, Comdr. IS HAZATA, H. Bacteriology XIII HXRAGA, Minoru Urology and Dermatology III HIRAI, Masrhito Maj, Gen, - Deputy Director III HONDA Expert I HOSOYAj So Sexology - Reg, Member I IBUICA, lienj:* Lt., Gen. - Director III ISHII, Nobutero Parasitology - Reg, Member I ISHIICAWA, Hitsuteru Medicine XXVI ISHIKAWA, Tomoyoshi Physiology XI I SHI.TARA, Fusao Director, Hygienic Lab. YIII ISIDATE, Morizo Chemical Analysis ’ ’ II INDUE, Takatomo Col, - Biologic Mfg, Div, III no, Y. Opthalmology XIII KAMBAYSHI, H. Lt. Gen, - Surgeon General III KANBAYSHI, Y. Vice Ad*teal - Director IX KANNO, S, Director, Bacteriological Lab. VIII ICAWAI, Samae Captain IX. KAVAXITA, Yosio Bacteriology - Reg. Member I KATAMURA, S. Botany XII ICAWAMUHA, Rin-ya Pathology IV, VI ICITAOKA, Masami Virology - Reg. Member I ICETASIIBA, Taiohi Director IV, VI KOBAYASHI, Rokuzo Bacteriology IV, VI KOBAYASHI, Yoab.ito Pharmacology II ICAGAYA, Y, Forensic Medicine XIII KOIDZUMI, T. Parasitology IV, VI KOIKE, K. Dean of College XIII KOJTMA, Saburo Reg. Member - Editor, Journal of Experimental Medicine I KOMINIAM, K. Ideologist XII XOYAMA, T, Clinical Medicine II IOJRIMOTO, Uzuhiko Bacteriology - Reg. Member I KUSAMA, Yoshio Hygiene iV, VI KOTSDKAIOS, R. Pathology - IV MATSUiCOfc M, Lt. Col, - Maxillo-facial III MATSUMGTO Pathology II KATBUSAia. Akira Maj. Gen,- Director V MIKE, Yukiharu XVIII MINAMIZAICI, Yushichi „ XVIII MINATO, A. Pharmacological Chemistry XIII MISANOU Major - Radiology III TOKYO AREA (continued) Name Associated with: Institution MISAWA, Takayoshi Allergy II jCYAGAWA, Yonegi Beg, Member - Dojinki Society I XEYAICE Pathology II dORIYA, Y, Electrical Engineer I 1AGAE, D, Col. - Surgery III KAGAN0, H, Parasitology VI NAKAKARO, Waro VII NIATO Major - Biology III NISHIKURA, Iv.ao Ghemo-therapeutics XI NISHINO, Chujiro Dean XV NOBECH, Kezio Director XI OGATA, Tomio Serology II OGAWA, Teizo Brain $esearch Inst, II OHMURI, K, Internal Medicine IV OKA, Haruraita Director XXJZ OSUZU, Hirobunii Co... - Internal Medicine III OTANI, Shoichi General XXVIII SAITO, Se Medicine Nihon Univ, Med, College SAKURASAWA, F, Director of Hospital Nihon Univ, Med. College SATO, K. Dermatology and Syphology XIII SEO, T, Surgery XIII SHIMIZU, Kentaro Brain Research Inst, II SHOJT, Yoshiharu Opthalmology II SUEYOSHI, Yuji Biochemistry IV SUWA, Keisaburo Col. - Pathology Neuro-nsychiatry II, HI TAHAKI, Y. Dean ‘ XOT TAICANO, Rokuro Bacteriology VI TAXIGAWA, K, Col, - Orthopedic Surgery III TAXITA, Kikuji Pharmacology II TAICURA, T. Pediatry XIII TAMIYA, Takio . Dean and Director of Toky$ Imperial University, Medical College. I, II TAMURA, Kenzo Pharmacology II TANIKAVA, K. Hygiene XIII TANAEASHI Col. - Urology HI TATSUI, Goro Colonel HI TODA, K, Col, - Plastic Surgery III TSUZUKI, M. Surgery • ’ II UCHIMURA, Yushi Director, Brain Research Inst. II UMEZAWA, H, Penicillin Research HI, II YABE, M. "BCG" Work HI, XV YABUTA, T, Agricultural Chemistry XII YABUTA, Jeijiro Director of Research XXVII YAMAGATA, S. Col. - Opthalmology III YAMOSHIliA, Hoshika Major ' Hi YAMAUOGHI, T. Lt, Col. - Director of Research XXVIII YAMAGUGHI, Maseyoshi XVIII YAOI, Hidetake Bacteriology - Expert I YAZAKI XXVI YOKOKURA, S. Rear Adm. - Assoc. Chief of Research IX WATANA.BE, Yoshimasa Bacterl ol nav APPENDIX Items of Interest Uncovered during tile Investigation of Japanese Medical Activities: The numbered items are specific ones considered to be of interest to medical men in general. This list does net nece- ssarily include all items 'uncovered, for the available literature was incompletely reviewed; due to language difficulties; some items that are not listed way be of interest to certain medical specialties. These numbered Items are followed by references, indicating the section of Appendix ,7A'5 and the report in which it is men- tioned... 1* Dysentery of unknown etiology occuring on Shikoku, Section I - Report of the same title. 20 Myopic hrobi m Section if -• Opthalmology ?)epta Section SXIII - Clinical Division - Opthalmology Section 3QCEV - Opthalmology Section XI - Myopic Problem 3• "Melanophoren hormone” in night vision.. Section II - Opthalmology Dept, Section XXIII - Opthalmology Section IX - Pharmacy 4* Insect Repellents. Section III - Pharmacology Dept, * • Section XVI - Pharmacological Laboratory (3,6, £ 7) Section III - Research Reports - Studies oil Communi- cable Diseases (2b) Section III - Research Reports - Other studies, Proph- ylaxis and Treatment of Malaria, 5. G-as Gangrene Vaccine. Section III - Manufacturing Division, Niigata 6/ B,G,G. Vaccines^ Section III - Army Medical College - Kanazawa Section III - Biologic Manufacturing Division Section VX - Kitasato Institute- (I) Section III - Research Reports - Studies on Internal Medicine {!)• Section III - Research Reports - Other studies, Tuber- culosis, Section 2XII - Bacteriology Dept, Appendix ”C” (continued) 6* D»C«G« Vaccines, Section XXIII - oiiiiio.nl Division - Opthalmology Section XXV - Takeo Institute for T.B. Section XXIX Section XVI - Hygienic Laboratory 7* Use of "Cepharanthln" in Treatment of T«B«. Section XI - Chemotherapeutic Institute Section XJV - Cepharanthin Section XV - S. Ota Section XV - Institute of T.B. and Leprosy Section III - Research Reports - Studies on Internal Medicine (2) Section III - Research Reports - Other studies, Tuber- culosis,, 8, To::: ic £ lye i c aiainc j?roia Human Cancer, Section XV - Medico-Chemical Institute National Research Council Projects of Japan, Section XVX 10. apparatus for Meinsuring Minute amounts of Nitrogen, Section XX - Biochemistry 11 • 0-Aminophenol Treatment of Tuberculosis. Section XX - Chemotherapy in T.B, Section XX - Pharmacology 12, Ant i-Malarla1 Druffs. Section XX - Pharmacology - Dirnethozy-S-diethylamino cthylaminoquinolino. Section XXI - Pharmacology - 2 Methyl-Morcaxto 6 chlor- 9-pyridino 3f2f and 2:3 quinoline chlor hydrate. Section XXI - Tropical Disease - Use of Lowered Oxygen Tension in Treatment of Malaria. Section XXII - Bacteriology - 2-Methoxy-6-chlor-9 (4- diethyiamino-cydohexyl-amino-acridin), Section XX - Pharmacy - Sulfadibrombenzene, Section I - Parasitology Section III - Parasitology Section IV - Parasitology Section III - Studies on Internal Medicine (3b & o)# Appendix "0” (continued) Electronic Studies, oection XE (15) Section XXI - R. Kimura 14. Dengue Virus - Cultivation and Vaccines. Section XXI - R. Kimura Section XXII - Bacteriology Dept, Section I - Dengue, 15. Le i shmanias i s, Section XXI - R, Kimura - Treatment of Le i shmanias i with Antinonyl hexcnate Section XXI ~ Pharmacology Section I - Parasitology Dept, Section IT - Colloidal Preparations of Various Heavy Metals (2), Sect5 on IT.i Research Reports - Studies in Pathologi- cal PieIds (5). 16. Schistosomiasis, Section XXI - Pharmacology - Treatment of Schistoso- miasis with Antinomy! hexonate. Section II - Colloidal Preparations of Various Heavy Metals (2), 17. Prosthetic Limb Studies. Section XXII - Orthopedic Dept, IS. Treatment of R, Infections. Section SXEY - Bacteriology 19* Leprosy Treatment«, Section XXIV - Clinical Medicine Section XV - Institute of T,B. and Leprosy Section V - Okuba Army Experimental Station (Neooyamine) Section VI - Kitasato Institute (1) 20, Wartime Research Commission, Section XXV 21. Education Requirement for Medical Degree - Igakusi Section 2321 Section III - Organization of Medical Dept,, Japanese Army (6). 22. Comments on Medical Education Section 2XEII Appendix "C" (continued) 23- Convers i on of. Sen Wet or _to Drinking Water-. Section IX - Pharmacy Dept. 24. Intra-Arterial Shock Injection Treatment, Section XIII - Chiba Government Medical College Section XVI - Hokkaido - Hot Springs Research Institute 25. Bone Marrow Extract for Anemia. Section XIV - Biochemistry 26* Goiirplemont Fixation Reactions in Malaria, Section XVI - Bacteriology Section III - Parasitology Section III - Research Report - Studies on Internal c'Icine, 27. Coll' racial Preparations of Various H cavy Metals * Section I - Colloidal Preparations of Various Heavy Metals (1), 28* Dried Biologic- and Pat Preparations* Section I - Colloidal Preparations of Various Heavy Metals (3,4,5* & 6), Section III - Biologic Manufacturing Division Section III - Research Reports - Studies on Communicable Diseases (11-1,2,3,*4 & 5). 29. Japanese B Encephalitis, Section I - Japanese B Encephalitis Section I - Encephalitis Virus Cultured in vitro 30. Infectious Hapatitis. Section I - Infectious Hepatitis Virus "Communin". 31. Section II - Pathology Dept. (1), Ishihara Color Charts, 32. Section III - Clinical Division - Opthalmology Ponecillin, 33. Section III - Ponecillin Research Committee Section XII - Nagac Institute Appendix "Cn (continued) 34* Array Medical College Research Reports. Section III - Army Medical College Research Reports 35. Studios on Water Supply Apparatus. Section III - Research Reports - Studies on Communi- cable Diseases (IV - 1,2,3 & 4). 36, Typhoid Foyer, Section III - Research Reports - Studies in Fatho- j-ufe-ioal Fields (9)e 37 • Treatment of Soa-sickne-ss and Air -sic lanes s, Section III - Research Reports - Studies in the Field c:r Otolaryngology,, APPENDIX !rD" Alpnanetical List of Institutions Screened (All Roman Numerals refer to Appendix "A" ) Agricultural Experimental Station Army Depot for Medical Materials, Yoga Army Experimental Station, Okuba Army Medical Gollego XXVII XXVIII V III Chiba Government Medical College XIII Educational Acquirements for Medical Degree •vvyr Hokkaido Imperial University, Medical School, Hokkaido Imperial University Sapporo XVI AVI I Institute for Infectious Diseases (TIU) Institute of Physical and Chemical Research Institute for PublickHealth VII XI Jikei-Kai University, Medical College XXVI Kanazawa Government Medical College Keio University Medical College Kitasato Institute for Infectious Diseases Kyoto Imperial University Aa. IV VI *'3rV*T iuU. Kyushu Imperial University, Fukuoka XXII Ministry of Health and Social Affairs Municipal Hygiene Laboratory, Tokyo XVIII ,VIII Nagao Institute XII Nagoya Imperial University Navy Medical College XXIII IX National Research Council XIX XIV Niigata Government Medical College Okayama Government Medical College Osaka Imperial University XXIV XXV Research Institute for T*B, Technical Intelligence Tohoku Imperial University, Sendai Tokyo Imperial University X XV II Tokyo Imperial University, Government Institute for Infectious Diseases Wartime Research Commission Appendix "D" (contingued) Inp-h-i*-a'Cos associated with Universities and reported raider University Heading; Moringa Penicillin Plant- III III III Army Medical College - Niigata Array Medical College - Kanazawa Criological Institute XVII ■JCV Aeronautical Medical Institute Research Institute for Acid Fast Bacillus Institute for Hot Springs Therapy XVI XI Chemotherapeutic Institute Me d i c o-Chemi c a1 Institute Bacteriological Institute Leprosy Institute Utizino Laboratory for Chemical Research Microbiological Institute JuA.X •vv T JU'*± rev XXV Talceo Institute for T\B* No reports rendered— Morioka Medical College Axaori Medical College Tokyo Phanaaoeutiol College Tokyo Charity Hospital Showa Ison Hospital Nippon University, Medical College Nippon Medical College I. TPICK) Eu:£P.IAL tn-JIViaSITY Governtient Institute tor Infectious Disease Manufacturing Division Parasitology Dept, Colloidal Preparations of Various Metals and Biologicals B. Encephalitis Vaccine B. Encephalitis Epidemology Dengue Acute Liver Necrosis due to Infection of Shellfish The Action of Immune Serum on the Japanese Encephalitis Virus culti- vated in Vitro, Infectious Hepatitis virus isolation and cultivation Dysentery of Unknown Etiology occurring on Shikoku TOKYO IMPERIAL UNIVERSITY Government Institute for Infectious Disease Manufacturing Department Director: Dr, Saburo Kojima Assistant: Dr, Hidetake Yaoi This department is responsible for the production of serum, vaccines, toxoids, and other biologic products. Results attained in the Research Dept, are immediately applied in this department. The laboratories have not been modernized. Samples of the products of this institute as well as cultures of strains of bacteria used and description of methods used will be forwarded under separate cover. Attached is a list and description of biologicals prepared. TOKYO IMPERIAL UNIVERSITY Government Infectious Disease Institute, Tokyo Parasitology Department Dr* Nobutaro Ishii The laboratory facilities are faily good in this institute. Dr. Ishii does strictly research work and is probably doing the most important work in Tokyo on medical parasitology. The fol- lowing subjects were studied: Kala-azar: Transmission studies. Has transmitted leishmania forms to barnsters by the oral and cutaneous routes. Reprints attached. Paragonimiasis: Dr. Ishii states that Dr. Yokogawos in Formosa has obtained good results in the treatment by means of emetine plus sulfanilamide. Drugs: 4* 4-1 - Diguaanidine-diphenylsulfide has proven very effective in the treatment of Kala-azar. Sulfadibrom-benzene found effective in the treatment of bird malaria (reprint attached) Sulfapyridine in experimental spirochmetosis recurrentis (reprints attached). Animal Helminthology: Reprints attached. COLLOIDAL PREPARATIONS OF VARIOUS METALS AND BTOT.OflTflAT.S Summary of work of Drs. Yonegi Miyagawa and Y. Moriya of the Government Institute of Infectious Disease. 1. Dealing with preparation of antimony powder colloid* silver powder colloid and other metal powder colloids. Antimony, silver or other metals (Cu, Au, Mg, Zn, Cd, Mg, In, Si, Sn, Pb, As, Bi, S, So, Te, Mn) are evaporated in vacuum by an electric heating unit and dispersed on the surface of crystalline dextrose. Photographs of the diagrams of the appara- tus are attached. The djy powder is stable in vacuum, heat resistant for 5 hours at 80 C and an hour at 100°C. It readily forms colloidal solutions in water having the same solubility as that of dextrose alone (AQ£ or more). The aqueous preparations of the antimony colloids change color slowly due to oxidation of the antimony. These various colloidal preparations have been used intra- venously in both humans and experimental animals in the treatment of certain protezean, metazean and rickettsial infections as described below. II. Dealing with the treatment of trypanosomiasis, leishmaniasis, schistosomiasis, clonorchiasis, filariasis, rickettsial disease and “lymphogranulomatosis ingriraalis" with antimony or silver powder colloid. (l) Trypanosomiasis. It is reported that intravenous admin- istration of 1.0 cc of 0.007-0.009 antimony powder colloid sol- ution to mice infected (presumably with Trypanosoma gambiense) at a time when the mice were definitely ill resulted in rapid de- generation of the flagellates and their complete disappearance from the blood after two hours. Clinical recovery was complete in one day. Comparable results were claimed in the treatment of horses suffering from surra (T. Evansi) or dourine (T. equiperdum), with doses of from 50 to 200 cc of a colloidal preparation. Excel- lent microphotographs show the reported degenerative changes in the trypanosomes. (2) Leishmaniasis. Data is submitted on the treatment of leishmaniasis in dogs and squirrels. Good results are claimed. The protocols suggest that there were fewer leishraania demon- strable in smears from the organs of the treated animals, that their spleens were smaller and the body weight greater. One human case of kala azar, eight months duration, was re- ported cured in 66 days by intravenous injections of the antimony colloid. Apparently the powder used was % and was given in doses of 1.0, 1*5, 2.5 — grams in 50 cc of water. There were no toxic manifestations. A total of ten doses were given in 2U days. A progressive improvement in the blood picture is tabulated. COLLOIDAL PREPARATIONS OF VARIOUS METALS AND BIOLOGICALS (continued) (3) Filariasis. Treatment of 8 cases of human filariasis using various doses of 1% or 3$ antimony colloid powder resulted in disappearance of the microfilaria. The higher doses employed sometimes caused anoremia, nausea, vomiting or coughing. The antimony preparation given to dogs Infected with Dirof- ilaria immitis is reported to have killed not only the micro- filaria but also the parent worm leading to embolic occulsion of the pulmonary artery. (4) Schistosomiasis. Five human cases of Schistosomiasis japonica suffering from diarrhea and malnutrition but without splenic or hepatic enlargement were treated with the antimony preparation. The 3$ powder was used in doses of 0.5, 1.0, 1.5 and 2.0 gm dissolved in 50 cc of water and injected intravenously every other day. The eggs in the feces disappeared after the fourth and eighth injection and did not reappear within a month and one half. Nausea, vomiting and coughing were occasionally observed. Hie patients gained weight and overcame their anemia. Seventeen similar patients treated with tartar emetic did not show such a favorable response although they were somewhat im- proved. Treatment of experimentally infected dogs followed by post- mortem examinations showed that adult worms and eggs had either completely disappeared or were degenerate. Four severely infected cattle were treated; three recovered completely and one died during the course of therapy. At autopsy adult worms could not be found and eggs in the tissues were degen- erate. (5) Clonorchiasls. Both dogs and human cases are reported to have been cured. (6) Typhus. During a small typhus outbreak (type not stated) in Tokyo in 194-3, seventeen patients were treated with a silver colloidal preparation. One (5.9$) patient died. Among 111 not treated 30 (29$) died. It was claimed that treated patients showed rapid clinical improvement. Antimony colloidal preparations were less satisfactory, since 10 (34*5$) among 29 patients so treated, failed to survive. (7) Lymphogranulomatosis ingrimalis. One case was treated with the antimony preparation with good results. COLLOIDAL PREPARATIONS OF VARIOUS METALS AND BIOLOGICALS (continued) - 3 - III* Dealing with the preparation of dry plasma, dry sera and bacterial toxins* This deals with drying plasma, serum and tub- erculin by vacuum at 37° Centigrade, Dextrose is added for reasons which are not self-evident. The following had been successfully maintained at room temperature for at least one years a* Suspensions of B, coli (mixed with silicates) b. Lymphogranuloma venereum Cold liver oil d. Red blood cells e. Colloidal suspensions of antimony f. Colloidal suspensions of silver g, Tubercle bacilli h. Tuberculin (for skin testing) i* Human sera (immune and normal) J. Hyperimune serum (diphtheriura; antitoxin titres checked before and after) k. Plasma 1* Quinine ra* Plasmochine n, Ricketosia IV, Describing preparations of fat powder colloid and its use. By intermittently spraying fine droplets of oil on warm dextrose crystal kept under a low vacuum, powdered preparations of cold liver oil, butter, neutral fat, etc,, were prepared. They contained from 1 - 3% of fat and could be added to water as 10-20$ mixtures. No fat globules were visible under the micro- scope, If the aqueous preparations are left at room temperature in the open air some visible separation of fat occurred after 10 hours. The 10-20$ mixtures were used for intravenous injections in humans in doses of 50-150 cc every 4th day. An interesting series of weight curves were accumulated showing considerable improve- ment in malnourished patients. V, Describing preparation of a "powder colloid of whole blood" and its use. Small amounts of whole blood are added repeat- edly to dextrose and dried in a vacuum between each addition. This mixture of dried powdered whole blood and dextrose has been put into solution and given to humans by the intravenous route for the treatment of anemia, both acute and chronic. With use of the larger doses, hemoglobinuria "occurred with no harm." VI, Describing preparation of "powder colloid of bacteria" and its antigenic properties. Bacteria disintegrated by various procedure such as freezing and crushing, "colloid mill method" "Weimarus" method and the supersonic wave method, are dispersed on the surface of dextrose crystals in the same amount to dextrose rproc^Bh mlXiaS’ dr7iDg lD 8 TO0UU" aBd repetitlon °f the COLLOIDAL PREPARATIONS OF VARIOUS METALS AND BIOLOGICALS. (continued) - U - VI. (continued) The preparations are readily soluble in water, forming Mtypical milky turbid suspensed solutions'1. It is claimed that studies in both humans and animals show the preparations to be more antigenic and less toxic than bacterial emulsions. Also the dry powder is more stable than the usual liquid preparations. Samples of these preparations are being forwarded under separate cover. TOKYO IMPERIAL UNIVERSITY Institute for Infectious Disease Prof. Yonegi Miyagawa and Staff I. Vaccine - Japanese B Encephalitis (Kitsoka) Vaccine consists of 10$ of 0,2$ formolinized (suspension) of mouse brain, (in saline). Two subcutaneous injections of 0.8 and 1,0 cc are given. One thousand individuals in test area vaccinated. However, no outbreak occurred so no human evaluation was possible. Serum from a greater portion of those vaccinated showed 1,000 to 10,000 neutralizing antibodies (per cc), (Neutralizing antibodies against 1,000 to 10,000 M.L.D. of virus). Vaccine standardization; Three doses of vaccine 0.3, 0.5, and 1.0 cc given to 20 gram mice at five day intervals. Two weeks later mice resist 100 or 1000 intracerebral doses, (of virus?) Five tenths cc of serum from vaccinated individuals inocu- lated subcutaneously protects mouse 12 days later against 100 or 1000 intracerebral doses. Monkeys receiving 10 cc similarly protected in five days. II# Epidemiology of Ja B. enoephaliti Kitaoka (1938) Tokyo Iji Shinshi' (weekly medical journal) reports isolation of virus in the field from culex pipiens var, pallens and aedes albopictus. Anopholos sinensis found on labora- tory experimentation to be a vector. Tranovarian transmission was noted. In regions where disease prevalent-antibodies were found in a "normal” man (80-90$, dog and pigs (85$), horses (98$), cattle (88$), These observations were in the Tokyo area. Sera from Hokkaido was negative. Sera from Kobe and Osaka showed a lower percentage of positives. The percentile difference showed a relation also the mosquito incidence. Active infection has not been noted in horses and cattle. III, Dengue. Claims for the isolation of a phenol susceptible angue virus, some years ago, with the same filtration size as the yellow fever virus have been made. A sample of this virus is to be forwarded under separate cover. Japanese studies with this virus showed no difference be- tween it and the Java and Malayan strains. A strain of dengue virus, isolated in 194-3 ty" injecting patients whole blood intra cerebrally into mice, is now in its III. Dengue (continued) 200th mouse transfer. (With this virus) Two to three per cent of mice show paralysis two to three weeks after injection (no en- cephalitis). Thirty-five hundredth cc of a 10% emulsion of the brain from the tenth mouse passage inoculated subcutaneously into humans, caused fever (39°) in eleven days that lasted for five days. A maculopapular rash lasting 20 hours appeared thirteen days post-inoculation. Patients serum 12 days after illness, produced (?) eucephalitis in 100% of the injected mice. Patients serum three weeks after inoculation neutralized mouse virus. The potency of the virus is constant between 10-A (.0001 cc) to 10-5.5 (.00005 cc). One experiment on human vaccination with 10% of a 0.25% formolinized mouse brain suspension (in saline) given in three doses of 0.5 cc, 0,8 cc and 1.0 cc at weekly intervals, protected against 0,2 cc and 0,7 cc subcutaneous injections of infected human serum. The control came down with a typical case of dengue. ' TOCTO BffigRIAL .UNItelSITY CroTernment* ‘liisTrbute"o'f* infectious Djs ease Acute Liver Necrosis due to Infection of Shellfish (Oysters and Kassels) Toxin has been extracted from liver of the poisonous mussels and oysters by Dr, Ahiba (Bacteriolody Dept, of Tokyo Imperial" University), It is v/ater and alcohol soluble and thermostable, Irmiune responses were not studied. Toxin injected into mouse, cat, doe;, rabbit, reproduced hepatic leison; not in rat. Cause of ratarosistance not known, Transplantation of normal mussels and oysters, to affected regions in spring*induced toxic prop- erties; cpnversely toxic shellfish^transplanted to other regions, lost their toxic properties after a few weeks. Studies of plank- ton, in affected reg:ons, were unrewarding. Reprints on the pathology and clinical findings are attached. TO:CTO -ONIYLHSITY Government TnsTitute for 'Infectious Diseases, Tokyo Ya s i o Kawakit a The action of Immune Serum on the Japanese Encephalitis Virus Cultivated in Vitro, A “so-called*7 Immune Horse Serum prepared by inoculation with lOfc mouse brain virus (Strain Kalinina isolated in 1935) over a period of five months, a total of approximately 2,250 cc was injected, inactivated virus in vitro and protected mice (S to 10 gm) in a dilution of 1-10,00£®,(100 M, L, D,), The Immune Serum also inactivated the virus in a culture flask - after 24 to 40 hours incubation at 35°C. Virus culti- vated in chick embryo (9 day old) v;ere likewise inactivated the Immune Serum, (0.3 cg of serum in 2,5 cc Tyrodes solution inoculated with one drop of infected chicle toain tissue.) The possibility of serum Therapy in Japanese encephalitis is indicated. No human experimentation is reported. TOICYQ IMPERIAL WIVmSTSY Government Institute for Infectious Diseases Dr. Masani Kitaoka Infectious Hepatitis virus isolation and cultivation. Experiments were conducted involving numerous animals and techniques which resulted with claims for the isolation of the virus and mouse infection (subclinically) with transmission from generation to generation. This virus appears to be about the size of the herpes virus. The active agent produced some degree of immunity in mice, and the neutralizing antibody was detected in the sera of human convalescents. This new virus does not be- long to the spontaneous pneung^tropic mouse viruses, nor has immunological relation to the influenza-A-virus through cross immunity tests. Cultivation of the virus in fertile eggs is claimed - Good- pasture Tecnnique is uGillzed. TOKYO DiHERIAL U3:T1VERS1TT Government Institute for Infectious Diseases Dysentery of Unknown Etiology occurring on Shikoku, Dr4 Sk Kojina Dr, Kojina is an authority on Enteric infections and Director of The International Salnonclla Center for Japan, Recently sev- eral new species of Salnonellac have been isolated in his labor- tory, Cultures of these are being obtained. The new species were not isolated in conjunction with any large outbreaks of dis- ease but were from' individual cases, questioned concerning dysentery, whooping cough and gonorrhea vaccines, Dr, Kojina did not consider then very effective, Con- cerning Influenza vaccination, he felt that the Japanese vaccine was effective- for three months. He has an ”A” influenza virus but his ”B” virus is no longer viable. A culture of the ”A” virus was requested from him. He in turn would like to procure the ”3”, An epidemic of dysentery of unknown etiology that first occurred in Takamatsu-Kagawa Prefecture, Shikoku Island was thoroughly discussed„ The disease has only been encountered on the one island and there only in the one prefecture and in that mainly in the city and towns surrounding Takamatsu, The disease first was brought to hie attention in 1944, atdwhich time there were 4,000 eases, with a thirty percent mortality. In 1945 there were 3,000 cases, witha twenty percent mortality. The disease is most prevalent from June to October, though eases occur up into December, Clinically, the disease is like Shiga dysentery, and exhib- its the following symptoms: Tenesmus, frequent stools (30 to 100 per day); stool bloody with mucous; severe headache; coated tongue; loss of appetite; fever, as high as 103; onset sudden; the incidence by age groups is high - in children under 10 and in the group over 30, A typical curve would bo as follows: Incidence; The course of the disease is severe in children and in the older Groups, Mid eases occurring and not being reported in the niddle aged group, nay account for the curve presented. The duration is iron one reek to ten days. ’Those with the severe type infec- tion die within ten days. Shigella shigg was not isolated from any patients. However, a paracolon-like organism, now called the •5Obayashi strain”, was isolated iron about 20 patienta. This organism is agglutinated by the patients sc-run in a dilution of 1 - 800, Shigaaand Fler- ner strains,wof known origin, are agglutinated only in the low dilutions of sera (1 - 100.,), The Obayashi strain is considered as the possible etiological agent, by the above. Cultures of this strain are being produrred. In therapy an oral vaccine, pro par eel with this strain, was tried in a few instances without success, A limited amount of sulfaquanidine, available and used, seemed to be effective,. However, insufficient drup did not permit large scale use. II TOKYO BIPERIAL UNIVERSITY. Medical College Institute of Pharmacology and Pharmacy Pathology Department, Serology Section Brain Research Institute Opthalmology Dept, Division of Physiotherapy and Internal Medicine TOKYO ILPERIAL UNIVERSITY Institute of Pharmacology and Pharmacy Headed by Prof, Kenzo Tamura. Associates: Prof. Ryotaro Asuma♦ Morizo Ishidate, Yoshito Ko bay a shi, and Kikuji T licit a. The last five years, due to limited laboratory equipment and supplies, very little new research was conducted. The fol- lowing subjects were studied: Digiconin: A glucozide of Digitalis isolated and structure determined by Tamua, Ishidate, Kobayshi and Tolcita. Use: Cardiac stimulant Action: Less toxic than digitalis Reprints and samples attached. Vita carroli or: ,lP~oxooamph yrv Synthesized by: Tamura, Kihara, Ishidate Use: CarcTjfaro Stimulant Action: That of stimulation Reprints and samples attached, Bufotalis: ”Senaon Extracted by Kobayashi Use: Cardiac stimulant Re print s attached. Estrogenic substances and Pituitary preparations. Reprints attached. Copy of Japanese Pharmacopoeia is being forwarded under separate cover. TOKYO IMPERIAL UNIVERSITY Pathology Department, Serology Section Dr, Tomio Ogata I. ,lCommunin,,, a ne?/ drug studied by Dr, Ogata is of bact- erial origin and used in the treatment of pyogenic infections and eczema. The clinical use of bacterial filtrate was suggested by the fact that small doses of such filtrates will inhibit the Shwartz- man phenomenon and Dr. Ogata believes the active substance pro- ducing the Shwartzman phenomenon stimulates the reticulo-endo- Thelial cells to produce a substance harmful to bacteria. The preparation of the filtrate is as follows. B. Coli are grown on Agar plates for 20 hours and washed off with saline, the Saline suspension is centrifuged and the supemate filtered through a Sietz or Berkefeld filtre. This concentrated filtrate is diluted about 100 times and given in 1 cc subcutaneous in- jections daily for 5 to 7 days. Approximately 1,000 patients have been treated with good clinical results and there are case protocols on about 300 cases. Dr. Agata believes the saline material is a carbohydrate and he and his associates are working on its isolation. Attached is reprint with a local translation of same and one vial of the drug. Reference is made to journal of Experimental Medicien, Jan. 1, 1936, Vol. 63, No. 1, page 59-68, Inhibition of the Shwartz- man Phenomena, by Tomio Ogata, M.D. II. Studies on the Precipiton Reaction are also undertaken in the Serology Section, Attached is a reprint on same. TO.lv.YO IMPERIAL UNIVERSITY, iEDICAL COLLEGE Brain Research Institute""’ This institute appears to be a very loose knit affair; an insti- tute in name only. It is directed by the professor of psychiatry Dr, Yushi Uchimura, Research work is divided into three fields ~ Anatomy, psy- chology, and hereditary Biology, The pyschiatry and neurosurg- ical w&rds of the University Hospital afford clinical material in connection with projects of the institute. Current projects are: Effects of the Atomic bomb on the Brain and Nervous System, Electroencepholograpliery studies on epileptics, on effects of drugs on the brain, and comparative studies of waking and s1e e ping states* Menta- development of twins. Histology of the brains of genius. TOKYO IMPERIAL UNIVERSITY. MEDICAL COLLEGE Projects for the Improvement of Vision (National Research Council) Opthalmology Dept. - Prof, Yoshiharu Shoji The major objectives and accomplishments of the National Research Council program are summarized in the attached reports submitted by Dr, Shoji. According to Dr. Shoji the myopia prob- lem in Japan involves 15% of the population. Within this group about 2055 have a myopia of three diopters or more and these are all due to elongated eyeballs and are incurable. Eighty percent have a myopia of less than three diopters and among this group one in five is due to spasm of accomodation which can be allev- iated by atropine. At present fewer people are developing myopia and this is related apparently to the fact that there have been fewer students doing close work. Professor Nakejima has been working with a hormone known as Mmelanophoren hormon” which is said to improve night vision in humans* Under separate cover, three books and a set of reprints re- presenting the work of Dr. Shoji, are being forwarded. TOKYO IMPERIAL UNIVERSITY Medical Department, Division of Physiotherapy and Internal Medicine* Prof* Takayoshi Misawa Investigations were conducted on allergic diseases and hot springs therapy. There is very little hay fever in Japan, prob- ably due to lack of windblown hay fever producing pollen. There is no ragweed pollen. Food allergy is not uncommon and the most common allergy producing foods in Japan were Eggplant, Bamboo sprouts, spinach, sea fish and buckwheat. Prof. Misawa believes the histamine content of their foods is the cause of their pro- ducing allergic symptoms• Treatment used is Vitamin D and Calcium Salt therapy. Injection treatment with antigens is not used, but Histamine injections are though to be beneficial. Reprints of Dr. Misawafs research work, published since 194-0, are forwarded under separate cover. Ill ARMY MEDICAL COLLEGE, Tokyo Clinical Division Biologic Manufacturing Division Parasitology Section • Physical and Chemical Laboratory Penicillin Research Committee Manufacturing Division, Niigata Dept, of Pharmacolog3^ Kanazawa Brance Field Manuals Research Projects and Findings Organization of Japanese Array Medical Dept, APJyiy MEDICAL COLLEGE Cl’ini cal Division Chief of Surgery - Colonel Nagae (Mayo 1935-1936) Surgery is done according to accepted procedures and no new techniques have been devised. There has been no experimental or clinical research of importance or of an original nature. Opthalmology - Colonel Yaganoto Original work carried out during the war consisted of stud- ies of color perception and extending the work of Ishahara by development of a new color chart for the detection of fatigue in color visionc Some work has been done on the use of the color blind to detect camouflage. Some thought has been given to the development of the so-called "6th sense" in the blind. Maxilo-facial surgery - Lt, Col Matsuki Plastic surgery - Col, Toda Urology ard dermatol cgy’H Col. Tanahashi Radiology - Maj, Misanou Internal Medicine - Col, Hirobunii Osusu The department of internal medicine has been working mostly on clinical research in tuberculosis and in carrier detections in typhoid fever. No significant research has been conducted. Orthopaedic Surgery - Col, 1C, Tahlgawa Battle fractures are handled by Skeletal wire traction and casting with windows. Wounds are left open and not packed. Secondary suture is used in suitable oases. Department of Neuro-Psychiatry - Col, ICeisaburo Suwa Located in separate building,Called the "Konodai Insane Hosptial". Bed capacity about 700. Patients nostly psyohotios with a few war neuroses and organic neurological cases, including brain injuries. It is worth mentioning that paresis accounts for only 5$ of admissions as contrasted with 19c/o in civilian mental hospitals. Treatment was being carried out along the line of accepted principles,. Careful records and statistics were being kept but there were no^research projects worthy of not e, ARMY EPICAL COLLEGE, Tokyo Biologic.Manufacturing Division Director; Col, Inouye Due to destruction of the buildings,%this section has moved its plants to Niigata and Kyoto, However, Col, Inouye and part of his staff remained in Tokyo, Vaccines used in Japanese Array are: (innoculations are given as indicated below) Typhoid, paratyphoid A, paratyphoid B - mixed - routine Smallpox - routine typhoid ~ Control measure in face of an outbreak Paratyphoid A •• u ,T " ” 51 w n Paratyphoid B - ?t " ” ’’ 11 iT Cholera - " Plague - *' " " " t? " Meningitis - u ,r 11 ff ” ” Typhus « ■» » ” " " 51 " BCG- - go all tuberculin negative soldiers Tetanus - Toxoid in experimental stage Gas Gangrene - Toxoid in experimental stage Cholera strains used were three isolated recently from an outbreak in Shanghai* Plague cultures were Isolated from four human cases in Burma and Manchuria. The BCG immunisation program has been in effect since 1942, Approximately 20-30ft of all men joining the Army received BCG, In 1945, the vaccines were prepared in a dry form to pro long their potency. They never came into practical use* Vaccines were manufactured in Japan, Manchuria, North China South China and Singapore, Procedures employed in producing vaccines are attached. Visits of the Niigata and Kyoto plants are anticipated. ARMY MEDICAL COLLEGE. Tokyo Parasitology Section, Dr* H. Osuzu Very little research work has been done on parasitic disease* The following subjects were studied: Fllariosis: Treatment of cases of heniatochluria with colloidal antimony plus oral administration of picric acid* Good results reported. Complement Fixation Reactions in Malaria: Specific anti- gens for P. Viyax, P. Malarial and P. Fallcparun are prepared from the plasmodia obtained from human cases. Antigen prepared in an Na OH solution and left at ice box temperature - good for one week. Claim test is species specific with no cross react- ions. Treatment of Malaria: Sheet attached. These subjects are covered in "A new Book on Tropical Medi- cine", edited by Prof. Dr, Miyagawa, 1945, which is being for- warded under separate cover. ARMY MEDICAL COLLEGE. Tokyo Physical and Chemical Laboratory Director: Col. Goto Tatsul Assistants Major Yoshikaru Yamoshina The laboratory equipment in general is very poor. This laboratory is primarily a control lab for the Japanese Array. Routine tests are made on water, milk and all drugs and chemicals bought by the Array for their use. Very little research is done. The attached report indicates some synthesis attempted in the laboratory. None of this material has been tried clinically. A copy of Japanese Army Pharmacopoeia will be forwarded under separate cover. ARMY MEDICAL COLLEGE Penicillin Research Committee This committee was organized in February 1944 and its Direction appears to come from the Army Medical College, Out- standing Japanese scientist from many fields are associated with it. The Nagao Institute did most of the original culture work where more than 1000 strains of P, Notatum were isolated-eleven of these were productive. Two strains (#233 & 176) have been utilized. Total coramerical production was refutedly 1500 gms. of finished penicillin per month with smaller amounts produced in research laboratories and some crude local production in other places. Commercial production is done in the Moringa plant at Mishiraa (capacity 1000 gm, per month). The finished product is crude according to our standards. Attached are photographs of the Mishiraa plant and a local translation of a report on research and production by Dr, H, Umezawa. ARHf MRDICAL COLLEGE Manufacturine Division - Mi igc.tr. Ail alun precipitated polyvalent tozoid-containing ¥. septiquo Cl. histolytica, Cl. wclchii, 01. noryii nay have some nori'tV Report of this v/orh in reprints to he submitted upon receipt hy this comittec. ARMY MEDICAL COLLEGE Manufacturing Division-Niigata Gas Gangrene Vaccine The attached formulae was furnished this office by the Japanese in lieu of the original reports which have been sought but not secured. Also attached is formulae for preparing Tetanus vaccine. Reference is made to Section III of Appendix "A" to previous reports. ARMY MEDICAL COLLEGE Dept, of Pharmacology Col. G. Tatsui Insect Repellents Worked on the production of D.D.T. Less than 1 kilo was prepared due to shortage of chlorine and benzene. It was found very effective against lice, but only sufficient quantity was available for experimental purposes. Information of use of D.D.T. by the American Army, was ob- tained thru News Agency from Germany in August, 1944> when Germans discovered it was being used on the skin of war prisoners. Prof. 0. Kaburaki and Prof, H, Mori at the Agricultural Dept, of Imperial University, prepared a small amount (after hundred grams) with which the Navy experimented. The medical department employed pyrethriura as the main in- secticide. Derris was not available to any great extent. The repellents used were lemon grass oil, Citronella and a mixture of Benzopheriene and Thymol, which were found very effect- ive against raosquites. Paris green and annon-arsenic compound Ferric Arson were used against mosquito larva. Attached is a summary of research on Synthesis of D.D.T, ARMY MEDICAL COLLEGE, Kanazawa Dr. M# Yabe, Director The use of B.C.G. in the Anay and the preparation of a better B.C.G. has been the prime duty of the above in his con- nection with the Army, Reprints of data have been procurred. Further data is to be submitted by mail. These items will be forwarded under sep- arate cover. d?J:i IjJDICAL OQLLdGlI, TOIdTO Field IIoiiucils * Ifairuals for distribution to Japanese nodical officers, staff officers and cnli steel personnel, dealiirj vith the subjects list eel beloi/, he.vc been col lee* L>eL. C.j.lC. delivered to A.l'.I.S., GHQ,, hPPAC, for translation and forwarding: 1, 3r.c exrxiinmbion methods for oontcfjious C.iscr/xs mid 1 or food ;;oisoiling• 2, Ixtliods for ins, meting irr.t, 3 • In .un 1 x mb ions. h,9 Field medicine, 5. Unit dcntc.l or.rc li mid too’: for mdicr.l officers, 6. 'iTopicr.l hygiene mid s mi it mb ion. 7. Fsdcliir.tr ic cxrxiinr.tions. o, Chcnicr.l dirfrrc detection net node, 9. Toxicologic enm.inmbions. 10, Sr.nit.ntion mid liyrimie in cold circc.s. ARM! MEDICAL COLIEGE, Tokyo Research Projects and Findings Attached is a list of the research projects of the Japanese Army Medical College and an English summary of the findings. Reference is made to the report on Wartime Research Commis- sion, Section XXX. ARMY MEDICAL COLLEGE, Tokyo Organisation of Japanese Army Medical Dept, Attached is information from the CD, Japanese Army Medical College on: 1, Total number of doctors, scientists, nurses and other officers and enlisted personnel, 2, Total number of hospital, laboratories and other fixed and mobile units9 3, Distribution of medical personnel and units in Japan and in occupied areas, 4* Medical education,before and during war, in the Army, 3. Tables, of organisation and equipment, of medical units. 6, Lists of casualties'during the war, with breakdown as to cause and effect0 and the curriculum of civilian medical schools before and during the war, for doctors, dentists and nurses. Answers to questions 2,3, and 4 are in the process of translation and will be forwarded under separate cover. IY KEIO uh'IYhlSITY L-JEDICAL COLLFGK Chemistry Dept, Department of Parasitology Anatomy Department Journal of the Faculty KSIO UNIVERSITY MEDICAL COLLEGE Chemistry Dept. Director: Dr, Yuji Sueyoshi Main investigations carried out on Transformation of carbo- hydrates from fats, on coagulation of blood, and on Vitamin and nutrition studies. Laboratories were destroyed during the war. Attached are reprints of recent research work. Importance of Linolic acid in Nutrition C - Ajitarainuse Transformation of Carbohydrate from fats Blood Coagulation Miscellaneous ICE 10 JMIVimsITT J EPICAL COLLEGE Anr.-t only D e p: TTnlent ~ oTfaJimas j?o lie. Anatomic a Jgpon i ca riiiG attached is a catalogue of the volumes of this -journal published since April 1941. journals are being forwarded under separate cover* ICIIO bHIVhhdlTY MEDICAL SCHOOL D e part merit” o :f Paras i t o logy Prof, Dr, T. Koidzumi Dr. ICoidzumi and associates have done most of their work for the past fourteen years on the properties of the toxic sub- stances of Ascaris. Subject covered were: Ascariosis; Collected papers on this subject being forwarded under separate cover. Provocative Measures in Malaria: It was stated that stibisan is very effective In bringing malaria parasites into the peri- pheral circulation. Much better than adrenalin. Fever Therapy in Paretics: Plasmodium ovole utilized. Stated to be more* effective than P, vivax or P, malarial. Also ' easier to control and to effect a~cure of thermal aria infection. KhlO D1T1TERSITY ?-EPICAL COLLEGE Journal of the Faculty - ,fkeio Igaku” •The attached is a catalog of the volumes of this journal published since December 1940 issue. Publication was discontinued after the April 1943 issue. The journals are being forwarded under separate cover. / li V ARHT ISCPEHBISM’AL STATION, Olcui&a Medical Section General hcuba ariit station This is an Ordnance installation; however, it Appears that rdnance was an overall Technical service to the Japanese, This station is divided physically and by fields of work, Mr instance, #6 Section is CWS (Screened by CWS representatives) #7 Section is medical, Its delegated function pertains to weapons helping to physics. The director is Major General Matsusaki (Medical), As a sideline G-en, Matsusaki was Interested in increasing the individuals vigor and sensual acuity lb is reported by otlv- Japanese that he accomplished little Hof or once is nado to Append in "A", Section V, #10 Section is Shipbuilding, There was a nodical staff but it is reported that it accomplished nothing. Those arc tin only additions • having medical groins. Weapons paining to physics have been reported unon by the Scientific Jit e All gen c c Survey, * J 010JBA AHkY 'iS&mm.mTZAL STATION NO, 7 (Medical Section) Presentation of work by Surgeon Major General Matsusaki and staff. The only subject of interest was "Neocyamine", This chemical is employed in photography as sensitizing agent to infra-red. The basic idea advanced by General Matsusaki et. al. is that if more infra red rays can be brought into the body a beneficial effect will be.produced. They have administered neocyamine to experimental animals and to approximately one mil- lion humans. It is claimed that the drug is beneficial in the treatment of frost bit, uninfected and infected wounds, carbuncles, erysipelas, pyemia, burns, tuberculosis, lymphadenitis, and lepr- osy. , The drug is known here as Koha A (Neocyamine), Approximately 300 chemical derivatives have been prepared. Of these about 100 have been tested in animals, Koha A is roportedlj7- non-toxic. The usual dose is 1 or 2 tablets by mouth daily or 2 cc (0,3 mg) of adsolut ion, intraver, ousIt, A number of laboratory and clinical reports were submitted in support of the claims made. Most striking are the claims for the successful treatment of the leprosy. Among 371 lepers treated 81$ were said to have shown evidence of improvement, A lengthy series of photographs to support the claim were presented. Attached arc reports in Japanese script concerning Koha A, Samples of the drug are forwarded under separate cover. VI KITASATO INSTITUTE FOP. INFECTIOUS DISEASE, Tokyo Dr, T, Kitashima, Director VII INSTITUTE OF PHYSICAL AND CHEMICAL RESEARCH Physiological Chemistry and Nutrition VIII MUNICIPAL HYGIENIC LABORATORY, Tokyo Municipal Bacteriological Laboratory X TECHNICAL INTELLIGMOE (Accession list submitted in periodic reports) ICITASATO INSTITUTE FOR INFECTIOUS DISEASE, Tokyo Director: Dr. T, Kitashina Assistants: R. ICobayshi, Bacteriology Y. Kusana, Preventive Medicine K, Nagano, Parasitology Y, Watanabe, Bacteriology I, Bacteriology Principal research work has boon clone on Tuberculosis, Leprosy Typhoid and para typhoid fever, and dysentery. Work on Tubercul- osis has been chiefly differential studies on bovine, human and attenuated strains, leprosy studies have been on the pathogenicity and transmission based upon WatanabeT.s ’*so-called” rat strain. Typhoid and paratyphoid ctucTies have been on prophylaxis, attenuate strains for vaccination and innocuikation versus oral vaccination. Dysentery studies were on prophylaxis, oral vaccin- ation, and di$f c-rentat ionw II• Parasitology Very little research has been done in the last ten years. Concerning schistosomiasis, the cow is the most important resovoir host and is more important than man in the transmission of S. laponicum. Lime nitrogen has been found effective in the comorol' of snails. Coir-erning mosquito control, the breeding of Aedes Albopiotus in pools in Tokyo Area has been controlled by means of a minnow Oryzius Latipus. Attached is a list of studies published during the last five years. Archives of the institute since 1940 will be forwarded under separate cover. It appfeafcs that such eminence as this institute once had is rapidly fading into the past. INSTITUTE OF PHYSICAL AND CHEMICAL RESEARCH Physiological Chemistry and Nutrition Dr. Waro Nakahara (From a report, Chemical Research carried out at the Institute of Physical and Chemical Research, dated 25 September, 1945, of Scien- tific Intelligence Section GHQ, AFPAC.) No military research was carried out. Dr. Nakahara stated that he had discovered a new specific dietary requirement,; a lactation factor made up of two constit- uents, one an adenylic acid compound and the other, peculiarly enough, ortho amino benzoic acid. Estimate by the interrogating officer was that Dr. Nakahara was a scientist of high orde and truthful. MUNICIPAL HYGIENIC LABORATORY. Tokyo Prefecture Municipal Bacteriological Laboratory I, Hygenlc laboratory and director Dr, Fusao Ishiwara, is a governmental and municipal institution serving public agencies, physicians, and private citizens. It is divided into four sections - one for water analysis, and one for food analysis, another for clinical analysis including X-ray, and the other chemical analysis. Production of biologies is limited to diptheria toxoid and dried plasma. Laboratory appears to be in a "rundown" condition. II, Bacteriological Laboratory, directed by Dr, S, Kanno, is a governmental and municipal institution which prepares biologies and conducts surveys,. Typhoid, typhoid Para typhoid A & B, Cholera, and Dysentery vaccines were prepared. Surveys for carriers of typhoid, paratyphoid A & B, Dysentery, Cholera, Diptheria, Meningiocci, and Plague were carried out. Research work was unimportant. IX NAVAL MEDICAL CQLLEGL Organization Preventive Medicine Department of Pharmacy NAVAL MEDICAL COLLEGE, TOKYO Director, Vice Admiral Yt Jicribayasi Research Organ ICenlcyuou "in Naval Medical College was established in Autumn, mf.— Object The study of the medical science and pharmacology which is necessary for the sanitation and hygiene of the navy. Organization Chief of research. Surgeon Near--admiral Osuka, Kanai, Yokokura, Staff of general affairs. Surgeon Germane'er--Watanabe, Ota. Departments. Number of Sect: cL' Department Hoad members System of Education Surgeon Capt, Yoslda, Murakama 3 Medicine S, Cap. ICanai, Ariga 3 Surgery 3. Cap, Tomita 2 Oto-rino-pharyngology S. Gap. Nakamura, Yosida Optlialmology 3, Cap, Eunakawa, Comm, Tanaka Dermatology 3. Comm. Nakauchi Hoentogcnology S, Rear-adm, Yokokura, Comm. Norioka 4 Bacteriology S, Cap, Miyaoy Arlma, Kauai, Comra, Hatakcyama 6 Hygiene S. Comm, ICiyohara, Toda, Nakamura 4 Pathology S. Cap, Murakami, Comm, Ota 1 Pharmacology S, Cap, Murahara 4 Plan i of study At the beginning sf the financial year each departmental head makes a plar over the thesis of the practical studios, viz: Sanitation and hygiene of the Navy, Prevention & Treatment a sickness and wound, Prevention of gas casualties# Publication of jic result of studies. (1) Konkyubuho, hod. Journal (2") ICaigun G-unikai Zasi, Research record. It is claimed that records were destroyed. The proceeding is extracted from a local translation. Education in the Naval Medical College 1# Type of students (1) The surgeon education (I class—beginning course) 6 months, . To Educate young doctors, who graduated at a medical college in that year, basic naval medicine. (2) Education for chief surgeon (Ilclass—Junior course) 6 months. To educate the surgeon lieutenant, to become the chief surgeon of the naval ship. (3) Education of a special medical course, (III class— senior course) 2 years. To educate a medical officer as a specialist. 2, Rcsc-arch at the -uj.lege (1) The department of research was established in the August lnA3* Naval sanitation and hygiene were studied. (2) Cless.ificaticn of the , tudies, Infectious disease and parasitcn disease in the tropic, prevention and treat- ment of the tuberculosis, battle wound, airial hygiene and hygiene of the submarine were principal topics. (3) The results of Research, The results of these studies wore published in the Kaigun Gunilcsi Zashi. The proceeding is extracted from a local translation. NAVY MEDICAL COLLEGE, TOKYO Preventive Medicine Surgeon Rear Admiral 3* Yokokura, Associate Chief of Research (This report complements Appendix A, Section IX of report cover- ing period from 22 Sept to 8 Oct 4-5) Navy personnel received the following vaccines as indicated: Typhoid, paratyphoid A, paratyphoid B - mixed Cholera Smallpox Diphtheria - anatoxin given to Schick positive young men BCG - to all tuberculin negative men Tetanus - not routine - used antitoxin Typhus - mouse lung preparation - not routine Meningitis - used as a control measure Dysentery - oral vaccine of little value, if any The diseases regarded as most troublesome in the Navy were Meningitis and dysentery. No satisfactory control method for meningitis was followed; sulfaguanidine was of some use in the treatment of dysentery. Capt. Kawai denied any important research developments in the Navy Medical College during the war. Attached are memoranda issued by the Japanese Navy Depart- ment for the control of contagious disease during the war. Of interest are the use of oral vaccine for dysentery (admittedly ineffective) and the spread of epidemic typhus among naval per- sonnel. Under separate cover copies of the following are being for- warded: a* Instructions given to Japanese Navy Medical Officers for the control of contagious disease, b. Copies of research publications from the Navy Medical College for the years 194-0-1945( sic) NAVAL MEDICAL COLLEGE - TOKYO Department of Pharmacy- Dr* Ryozo Hayashl* Lt. Coradr*, Acting Chief This report compliments Appendix A, Section IX of report on period from 22 Sept to 8 Oct 4-5*) Investigation of interest ares The preparation of a sodium hypochlocite solution by elec- trolysis. Reprint attached. Study of 2-Methoxy-6-chlor-9 (4-diethyiamine-eye1ohexyl- amino-acridin) as a substitute for atabrine in the treatment of Malaria. Reprint attached. The preparation of drinking water from sea water by utili- zation of electric charge of synthetic resin "Qrgacid.” At 1st Supply Depot (Naval Arsenal) work was accomplished in the use of pituitary gland extract from fish and cattle. Claims have been made that when this hormone is injected, it improves night vision. Reprint attached and sample of drug under separate cover. Outline of training program in Pharmacy attached. XI DESTITUTE OF PUBLIC HEALTH. Tokyo Outline of Institute "Myopic Problem" Chemotherapeutic Institute, Ichikav/a, Chiba INSTITUTE OF PUBLIC HEALTH - TOLTO Outline of Institutc Attached is . general outline of this Institute giving its history, physical installation, organisation, stuff, duties and responsibilities, INSTITUTE OF PUBLIC HEALTH Director K, Nobechi "Myopic Problem" Dr. Ishikawa. Tomoyoshi (Physiologist) Member of Institute of Public Health within which is a "Committee for the study of the Myopic Problem" headed by Dr. Ishikawa. The committee was formed in 1939 to study 5 problems: I. Relation between myopia and constitution. II. Influence of poor lighting on development of myopia. III. Influence of general hygiene on myopia. IV. Hereditary factors in myopia. V. Nutritional factors in development of myopia. Kondo. Tadao (died 1944) opthalmologist did a great deal of work on the eyesight problem. Much of his work was published and is in the attached journals and papers. Ishikawa (above studied the effect of the various type charac- ters and has shown that square type is less fatiguing than the cursive type. Attached are new Journals and Papers which deal with the problem. INSTITUTE OF PUBLIC HEALTH Chemotherapeutic Institute, Ichikawa, Chiba Directors Dr* Shuji Hassegawa Secretary: Dr, Iwao Nishimura Chief Attached Hospital: Dr. Sheichi Arifuku Outline of Activities of Institute is attached. The curative and preventive properties of Cepharanthin are outlined in the attached summary. It is claimed that this drug is effective in the treatment of leprosy, whooping cough, as well as Tuberculosis. Recent work has indicated its value in the treatment of allergic conditions, particularly Asthma. A summary of this more present work is also attached. Under separate cover is a book on the experiments carried out at the Institute, as well as samples of the drug. XIX. NAC-AO INSTITUTE XIII. CHIBA GOVERMKEKT MEDICAL COLLEGE NAGAO INSTITUTE - TOKYO Dr. Kominiam. Director* Dr. Yabata, Chemist* Dr. Hatsuta. Ass»t. Chemist; Dr. Kawamura, Botanist. Mr, Okabo. Manager. This is a commercially supported laboratory for maintain- ing fungus and yeast cultures for use in industrial fermentation processes. It appears to be modern. Abstracts of methods and research since 1940 is attached. Reference is made to Report Penicillin Research Committee attached to periodic report of 22 Sept 45 to 8 Oct 45 Appendix A section III. Attachments s Reports on NAGAO Institute. The View of NAGAO Institute. The stock cultures at NAGAO Institute. The sorbose fermentation (II) by T. NEHIRA, at the NAGAO Institute. The sorbose fermentation (l) by T. NEHIRA, at the NAGAO Institute. The extraction of Flavin (Vitamin B2) from Elemothesiura Ashbyii. R. OKABE, Y. HATSUTA, and 2. Veda, at the NAGAO Institute. GOVERNMENT MEDICAL COLLEGE - CHIBA Dean Koike Staff associated with university: SBO, T., Prof, Surgery MINATO, A,, Prof, Pharmacological Chemistry AISO, K,, Prof, hygiene TANIKAWA, K., Prof. Hygiene SATO, K,, Prof, Dermatology and Syphology ITO, Y,, Prof. Opthalmology AKAMATSO, S., Prof, Biochemistry TAKURA, T., Prof. Pediatrics HAZATO, H,, Prof. Bacteriology KAGAYA, Y,, Prof. Forensic Medicine Research projects includes Intra-arterial shock injection treatment by SEP, et al — Sodium iodide, glucose, sulfonamide, sodium salicylate were in- jected in a variety of clinical diseases resulting in good re- ports. However, specific results are questionable. Vitamin A extraction from subcutaneous fat of seals by MINATO yielding 180 International units per gram of fat. The liver contained only one third as much in contrast with whole liver. Preservation of meat and fish with a culture of B. Butyercus for one and one half years; however the accompanying odors from the preserving agent has defeated the method for practical use. Research on penicillin has been carried out by TANIKAWA in connection with the Penicillin Research Committee, Reliable syphilis rates were secured from SATO which were higher than those in U,S, There has been no sensitivity to Atabrin encountered. Research on Enzymes has been done by AKAMATSO. Attachments: Results on 70 cases of intra-arterial shock injection treat- ment (Reprint in Japanese and English Summary) Penicillin Study. KIT NIIGATA GOVERNMENT MEDICAL COLLEGE Cepharanthin Pathology and{Para s it o1ogy)Dept, Pathology Dept. Dept, of Biochemistry Clinical Division NIIGATA MEDICAL SCHOOL Cepharanthin Dr, Takashi Hashimoto (Dermatologist) - Dean of School Extensive work was accomplished on the use of "Cepharanthin11 in skin T,B,, particularly Lupus vulgaris, with prolonged re- mission or apparent cures. No definite results determined in its use in chest T,B. "Cepharanthin" is an alkaloid of Wisteria Root grown in Formosa, It is manufactured by Kaken Seiyaku Mfg, Co,, Tokyo, under the supervision of Prof. Hasegawa of Institute for Infectious diseases, Tokyo. Reprint attached. Samples under separate cover. GOVERNMENT MEDICAL COLLEGE - NIIGATA Pathology Department Dr. T, Ito, Head of Dept* Dr. Ito serves in the capacity of pathologist and para- sitologist. No parasitological research work has been conducted by him in the past few years. Clonorchiasis: A large Clonorchis area exists in Niigata prefecture but only a few clinical cases are now being seen. People are familiar with the infected fish and do not eat them. Paragonimiasis: There are 4 rather restricted areas in Niigata prefecture, namely (l) Imar-mura (Higashi Kubiki district) (2) Ookambara-mura (Nakakambra district), (3) Nanatani-mura (Nakakambara district), (4) near Toka-machi (Nakauonuma district). Wet tissues of Paragonimus infected brain are being sent in under separate cover. GOVERNMENT MEDICAL COLLEGE - NIIGATA Pathology Dept* Prof* T* Ito, pathologist Prof. K. Akazaki, pathologist The department has been particularly interested in brain tumors and reticulum cell Sarcoma* Dr. Ito studied under Dr* Stevenson at New York Hospital until 1941. Approximately 100 autopsies and 300-400 surgical specimens are examined each year. Of this material about one half is re- ceived from the Medical School Hospital and the remainder from outside sources. Approximately 1/4 of the cases examined at autopsy were tuberculosis and l/4 psychiatric, during 1944p Tsutsagamachi fever is indemic in this area* Publications were suspended in 1943 but prior papers and selected interesting autopsies were requested and will be sub- mitted under separate cover* NIIGATA MEDICAL COLLEGE Department of Biochemistry Prof. N# Ariyama, Head of Department No research work was done during war, but before the war the department had 10 to 12 assistants. Prof, Ariyama spent two years at Washington University, St, Louis, studying with Dr, Shaffer and has been at his present post for 15 years. Prof, Ariyama claims to have isolated a blood forming sub- stance from bone marrow. The work was done before the war, has not yet been published, since the starting material, marrow, was not available in Japan in sufficient quantity during the war, Prom over 100 kg. of fresh narrow, about a gram of mator- ialwas isolated. Experimental aencmia, caused by bleeding in animals, is cured by a single injection of 0.1 mg. per kilo. He believes it stimulates the bone marrow. The bone marrow is extracted by water or dilute alkali, and precipitated many times by alcohol and acetone, and color removed by bone black. The material, a white powder, is nit- rogen free, produces reducing sugar on hydrolysis, part of which is fermentable, is dialysable thru a semipemcable mem- brane and is thought to bo a carbohydrate. It is non-toxic. Prof, Ariyama desires to repeat tho isolation of the material .and test its action on humans before publishing, as insuffic- ient pure material is available at present to complete the determination of the chemical structure. Attached is a summary of this work, prepared by Dr. Ariyama as a prelimary report for publication. Ten reprints from the department, published since 1940, ached* Attachments: .(1 ) Biochemical.. studies* on d-Riboso, with special reference to the Mechanism of Absorption of Sugars from Intestinal Tract, '~'(2)Studies on.H-Glycosidos, Part II ... (3)Studies on N-Glycosidos, Part I (A)Studies on Metabolism of a-Kotonic Acids* (5 )Studies’' on the Pasteur Reaction in Muscle, * - (o)Ubcr Einen Inhibitor dor Glyoxalasc. (7)Tho Reactions of Sugars with Amino Acids under Mild Conditions, (8(Studies on Hydrolysis- of Desoryribo-Nuolcotides and Nucleosides, ' ‘ " {9)Prccipitation of Nucleotides and Nucleosides with Salts of Heavy Metals. (10)Studios on Tumour Glycolysis, NIIGATA MEDICAL SCHOOL Clinical Division Dept, of internal Medicine Prof. I. Shibeta Investigate and published a report including the typing of pneumonia in Japan. (Reprint attached) Studied the daily Vitamin B requirements on Japanese pri- soners of war. Findings agree with American workers. Summary attached. Prof. Tasaka Conducted extensive studies on body temperature including influence of environmental temperature on cardiac and respira- tory activity, on skin temperature of extremities and reaction of the body to fever in various diseases. Attached are summaries of unpublished work, on vitamin and temperature studies and the effect of acute hemraorage on velocity of blood flow. Dept, of Surgery - Prof. M. Nakata Dept, of Radiology - Prof. S. Nosakio No research work has been done in these departments. • SQKP I'gQTQ aj. .^COLLEGE, Sendai Dept, of Pathology Parasitology Dept. Bacteriology 0t # S. Ota Institute of Tuberculosis and Leprosy Institute of Tuberculosis and Leprosy (Investigation relating to Diabetes) Clinical Division Medico-Chemical Instillvce Pharmacology TOHUKU IMPERIAL UNIVERSITY MEDICAL COLLEGE - SENDAI Dept, of Pathology- Dr. S. Nasu, Associate Prof, of Pathology- Dr. T. Hoshida, Prof, of Pathology (absent in Tokyo) Dr. S, Matsuoka, Neuro-pathologist Dr, S. Yatsuyanagi, Assistant Dr. Hoshida has been active in experimental research related to chemical Carcinogens. Special tissues including example of beri-beri heart was selected and will be forwarded under separate cover with depart- ment research project reports. TOHOKU IMPERIAL UNIVERSITY - SENDAI, JAPAN Medical College Pathology Department Dr* S, Nasu, pathologist Dr. S* Yatsuyanagi gives the lectures in parasitology at this school but was sick at the time. Dr* S, Nasu, a patholo- gist was interviewed. No parasitological research was being done at this school - only lectures to students. Paragonimiasis: Three cases of paragonimiasis in this in- stitution in 3 years. Malaria: No malaria but $ species of Anopheles in Niyaga prefecture. Clonorchiasis: Twenty years ago there was an area a little north of Sendai in which there were numerous cases of clonor- chis infection. Infections were obtained from a small variety of carp, know as Puna. People discontinued eating of this fish so that there are new practically no cases. Wet tissues on clonorchis cases are being sent in under separate cover. T OH OKU IKPHKXAL UiiTVDRSITY - SENDAI Mc.Gc.hiko ICuroyc., Prof. of Brcfcriology Working on the frc.ctionc.tion of E., tynhi, by chemical means. Has isolated, a polysacchiricic tonic fraction, Antigenicity un- determined beyond precipitin tests. Possible purpose of work, in nature of other fractionating work done, to use fractions for imn unogcnic purposes, working on fractionation of T,B, (This latter completely covered in U,S,) Reprints of previous work are cataloged by title and will be forwarded under separate cover. Working on isolation of Penicilliun notatum and preparation of penicillin. Toadies Bacteriology in die University. TOE OKU IIZPEEIAL ULIIVEtlSITY, MEDICAL G OLLEGrE - Sonde ?rof. S, Osato Dr. Osato has only recently joined the staff of the Sendai School, Before this, he was wording in the Kanazawa Medical College and all of his published research v/orl: will be obtained in the journals of this college when thy are collected. At present Dr. Osevto is interested in the following 1, Expcr i: ,cnt a 1 ly produe ed insomnia AninaIs which arc kept awake to the point of ex- haustion and death, can be kept alive several days longer than usual by the adninistration of Vitmin 2. The Effect of Hot Springs on Jfic animal body When experimental animals arc exposed to natural hot springs, the reticulo-endothelial system of the subcutaneous tissues can be shown to have an increased phagocytic activity. 3• Treatment of Tuberculosis DiV Osato obscrvcd more than 100 tuberculous patients showing all types of pulmonary involvement who were treated with ccpharanthin. In his opinion, the treat- ment was either of no 'benefit or was injurious. He is now observing the effect of rhodinic acid on tuberculosis, This ten carbon chain acid cones from the barl: of r, tree cornonly hnown as “Taiwan Sliinoki” in Formosa, According to Dr. S, Hatsura in Formosa the acid was effective in healing 20$ of patients with open Tuberculosis* Dr. Osato states that the preparation has come value in the treatment of tub- erculosis. The drug is irritant to the gastric mucosa and is dispensed in cod liver oil or some ar- omatic oil to offset this effect,’ It is given in a dose of 2-3 cc by month, every day for six months or more. A specimen of the commcrcially prepared drug was se- cured and is being forwarded under separate cover. TOHOKU IMPERIAL UNIVERSITY, Sendai Institute of Tuberculosis and Leprosy Directors Dr. Taiao Kumagai (Also Pres, of Univ.) The Institute of Tuberculosis and Leprosy is housed in a new building donated by a private industry and completed in 194-3* Although small, the Institute is modern in appearance and equipment* The staff, under Dr. Kumagai* is chiefly con- cerned with tuberculosis but leprosy is studied by Dr. Saburo Sato* whose work is mentioned below. Dr. Kumagai discussed several salient features of the tuberculosis problem in Japans 1. The peak death rate in Japan proper lies between the ages of 15 - 25* Formosans, however, have a tuberculosis mortality curve of the same character as that of Americans. Dr, Kumagai points out that Japanese eat less fat than any other people in the world. He offers the opinion that the tuberculo- sis death rate in Japan is a reflection of the di- etry habits of the people and he stresses the need for a high fat diet in treatment. 2. Twenty five years ago the tuberculosis death rate among rich Japanese was two and one-half times that among the poverty stricken masses. At present this situation is completely reversed and is re- lated to industrialization of the nation. 3. Cepharanthin administered to both tuberculous an- imals and humans was of no value in the treatment of the disease in the experience of the staff of the Institute. Dr. Saburo Sato presented two findings of interests 1. Koha A, referred to in a previous report under Okuba Experimental Station as a therapeutic agent for leprosy, was without value in the experience of Dr. Sato, who gave one milligram intranenously twice a week for six months to 60 lepers. 2. Dr. Sato had previously reported localized infect- ions in chickens inoculated with leprosy bacilli. During the war Dr. Sato demonstrated that by repeat- edly inoculating excised and emulsified leprotic nodules from humans into the breast muscles of a chicken, a generalized formation of nodules con- taining acid fast bacilli resulted in six months. Reprints from the Institute are being forwarded under sep- arate cover. TOHOKU IMPERIAL UNIVERSITY Institute of Tuberculosis and Leprosy Directors Dr. Taixo Kumagai (Investigation relating to Diabetes.) In addition to the work on tuberculosis and leprosy which he directed and which is described in another report. Dr, Kumagai is also interested in diabetes. He claims to have demonstrated the insulin effect of pancreas before the work of Banting, He has since been interested in finding other substances which will lower the blood sugar level in diabetics. This year Dr, Kumagai presented to the Tohoku Medical Assoc- iation his finding on a chemical which he extracted with ether from the leaves of a plant specified as Sathyrus palustris L. var macranthus 9 white) Fomald, When this chemical is given by mouth to animals it lowers the blood sugar. Dr. Kumagai also gave it to 25 diabetics. In mild cases the blood sugar was lowered. In severe cases no effect was noted, A report was requested to be forwarded* TOHOIOT IMPERIAL UNIVERSITY, MEDICAL SCHOOL Clinical Division Y, Yoga, Prof, of Radiology Dr, Koga is well lenown in. Japan for having developed the Japanese use of phot or o int g cniagraphy in mass chest surveys. Most of his work in this field was done before the war, partic- ularly in 1938# During the war, his research activities wore cut down con- siderably by a great reduction in the size of his staff. His department has been working however, on research into; 1. Tissue culture methods 2. TIic cTrcct of irradiation on tissue culture 3. Effect of irradiation on inflmiatory less ions and on (3 uricr cultures. General Surgery Prof, H. Huto, General Surgeon Prof, A, ICaturo, Neurosurgery Clinical research has been done durin , the war. Dr, Muto has, together•with his staff, /o.Iced on the surgery of Tubercu- losis of the lung and on gastrointestinal operations for mal- ignancy. Prof, Katuro and his staff have been working on the surg- ical aspects of epilepsy. They have done nany pro and post op- crativc electro-oneophalograns. Prof, I, Kihi, orthopedist, has been conducting clinical investigations of chronic shoulder disorders. Reprints of all their papers since 1940 arc being forwarded under separate cover. However, none of the work scons important. Medical Dept, Dr, Toyojiro Mato, Prof, Emeritus of Medicine, 63, one of the leaders in Japanese medicine, retired in 1942 to direct the Institute of Aviation Medicine at Tohoku, Was founder of avia- tion medicine in Japan and has worked on the problems of avia- tion medicine for many years. The work of the Institute was done in cooperation with the military authorities and financed by the government. They have been working on the usual problems, using animals (dogs & rabbits) They have a large pressure eh ember and a centrifugal apparatus and numerous laboratories for the study of blood chemistry. Their work has been published, with the exception of the most recent investigation in the Japanese Journal of Aviation Medicine, copies of which were obtained. (This info passed to ATIG) Dr, F. Kurokawa, Prof* of Internal Medicine, specializes in gastro-enterology, is one of the best workers in this field in Japan and has published a text book (1936) on X-ray diagnosis in gastro-enterology. Is now working on a study of the treat- ment of liver toxicities using levulose and Vitamin K. Has de- veloped a micro method for liver glycogen determination. Reprints of all his published work were obtained including a review of Japanese gastro-enterology, published in 1943 > which will be forwarded under separate cover* TOHOKKU IMPERIAL UNIVERSITY - SENDAI Medico-Chemical Institute Prof# H. Masamune, Director Extensive studies of biological activity and structure of carbohydrates and the glycoproteins# More recent work on the isolation of a toxic glycidamine from human cancer tissue, which is also believed a product of normal tissue# Structure not yet completely determined# Sum- mary attached. Blood group substances have been isolated which are be- lieved to be of carbohydrate nature related to chondridin. Attempts at the synthesis of blood group substances have been made# Summary attached# Attached are reprints of recent work as well as a summary of his carbohydrate chemistry given in 1943 before the Japanese Chemical Society. TOHOKU IMPERIAL UNIVERSITY. Sendai Medical Institute Motoo Terasaka, Prof, of Pharmacology Came to Sendai, March 1945, was previously Professor of Pharmacology at Nagasaki University of Medicine, where his re- search work was done* Research work on Pharmacology of Uterine Action, Effect of digitalis on the heart. Pharmacology of Ephedrine, and Chemo- therapy of (bird) Malaria, and a study of the action of diph- theria toxin. Attached are 15 reprints of work published since 1941. (Suggest titles be translated and listed). 2fVT EOIHCAIDO BQEIRIAL UNIVERSITY, NEDICAL COLLEGE. Sapporo Bacteriology Pharmacological Laboratory Nakamura, Bacteriology and Immunology Hygienic Laboratory Clinical Division Hot Springs Research Institute Pathology Dept, Biochemistry HOKKAIDO IMPERIAL UNIVERSITY, SAPPORO, JAPAN Medical College- Prof, of Bacteriology, Yutaka Nakamura Complement Eixation Reactions in Malaria Tested 30 patients with induced P, vivax malaria. All cases were cither positive or doubtful. Antigen prepared from human cells infected with P, vivax, Method of'preparation being summarised and sent under separate cover. HOKKAIDO IMPERIAL UNIVERSITY - Sapporo, Japan Medical College Pharmacological Laboratory Prof. Takeo Masaki, Head of Department The following studies were conducted under his direction; Subjects of Investigation 1, Pharmac o1og i o studie s on : Dihydrochavibetol Object To apply to medicinal purposes, Duration 19^3 A substance similar to Chialacol and found less toxic than creosote. Prepared from Oinnamomum Camphora. Use: Abdominal disinfectant such as dyspepsia. Externally as skin disinfectant,. No clinical study made, laboratory studies only on rabbits and mice, and freg. Action: Similar tc guaiacol and less toxic. Incl; Reprints an3 sarnie. 2,Skin irritant action of the principles of peppermint oil Rational use of pep- permint oil as skin irritant. 1943 Incl; Repent of studies. 3.Shin irritant action the volatile oils and their principles. To study the relation between the physico- chemical properties and the olein irritant Cxi?co t R. . 1943-1945 Result; The active principle of volatilve oils causes the irritation. The more volatile an oil the more irritating. Physical properties include the melt- ing points, solubility and volatility. Volatility is essential for the skin irritation action. 4•The anthelmintic action of the principles of the pep- permint 'oil, especially of menthol. Valuation of peppermint oil as anthelminthic, 1938-1943 Studios made on dogs, and cats found to bo very effective. Pound more effective than santonin. Large doses may be given and less toxic than santonin in largo doses. Pound 100% offoc t ivc on As c aris• Subjects of Investigation 5, Action of drugs on louse. Object To study the mech- anism of louso-kill- ing action of drugs. Duration 1944 Result; Essential Oils found effective, mixed with Boris Alba, This powder is found in Hokkaido. Talc may be used similarly, Boris Alba as a powder is found very porous, adding to its absorption properties. Use: Dusting powder. Insi; Reprint (Essential (ilapht ha Ionc-2 rf]o »Boris Alba-50$ Sample; Lausan 6, Benzene-Naphthalene method, for the exterminelion of lice. Ext erminato lice. > 1944 Naphthalene in nzen • 2rfjo solution. Clothing sprayed and put in chest for 32 hours. Use: Spray, Host effective and convenient for use. Incl: Reprint 7, Comparison of the louse- killing effects of various evaporable substances, Incl; Reprint, To study the relation between the pfcrysico- chemical properties andthe louse killing effect, 1944-1945 B, Pharmacologic studios on Lucidusculin, a principle of Aeon!turn Lusidusculum (Nakai) To study its clinical applicability. 1940-1941 Studjr made on rabbits. No clinical studies. Use: As an anti-diuretic Incl; Reprint 9, Pharmacologic studies on Valuation of stigmata 1942-1943 the diuretic principles of maydis as a diuretic, Stigmata Maydis, Stigmata Maydis is a powdered extract prepared from corn silk. Use: Diuretic, Being used in Europe, Incl: Reprint 10,The influence of quinine upon the function of ret- ioulo-en doth dial system. Research into the cause 1940- of the preventative 1942 effects of quinine on in- fection, (R c s pirat ory) Small doses stimulates, large doses acts as a depressant. Average dose: 0,003 - 0103'mg/Kg per OS Incl: Reprint Subjects of Investigation 11. On the influence of the room temp, upon the quantity of urine in a whole day. Object Duraction To judge exactly the 1942 diuretic action of drugs on the rabbit. (Particularly Stigmata Maydis) High temp: Little diuresis Low temps Increase in diuresis. Constant temps necessary when studying action of diuretic drugs. Incl: Reprint Enclosures: Reprints 1. Pharmakologische Studien Uber Dihydrochavibotol. 2, Uber die hautreizende Wirknmg des Pfefferrainzols • 3* Vergleichenao untersuchungen uber die pharraakologischen Eigenschafton einiger Anthelminthika und der zur Terp- enreihe geherigen Substanzen; sowie Wurmabtreibungsver- suche mlttels Menthols an Hunden, 4, Action of various drugs on the louse. $. Ueber cine neue "Benzol-Naphthalin" Methods zur Bekarap- fung der Kleiderlaus. 6, Comparison of the louse-killing effects of the various evaporable substances. 7, Pharmacologic studies on Lucidusculin, a principle of aconitum lusidusculum (Nakai) 8, Pharmacologic studies on the diuretic principles of stig- mata Maydis, 9, The influence of quinine upon the function of reticulo- endothelial system. 10. Study on the effect of room temperature upon the urine output in a day. 11, Manual on Drugs of Northern Japan (2), HOKKAIDO IMPERIAL UNIVERSITY, SAPPORO Dr, Yutaka Nakamura, Prof, of Bacteriology and Immunology Has visited the United States twice, most recently in 1922, at which time he visited in Boston and New Orleans, following studies on Yellow Fever, under Rockefeller sponsorship, In a Japanese Colony in South America. Primary interest is in Immunology and especially in Tissue Immunity (has the book of Kahn, ’’Tissue Immunity”, but has not read same.) Recent research on viruses diseases (vaccinia) and rick- ettsial diseases. Claims that by cataphoresis an antigenic im- munogenic protein fraction can be obtained from vaccinia. Claims that innoculation of blood of Tsutsygamushi patients testicularly into rabbits isolated virus which may be serially transferred, enhancing virulence. Has applied cataphoretic studies to some members of the Salmonellae group especially gaertner!s bacillus and Sal enteritidis. Is working on penicillin isolation of strains. A weak, impure product has been produced, experimentally. Research in general hampered by a lack of animals. Reports on work published in the Journal of the university are to be forwarded separately. HOKKAIDO IMPERIAL UNIVERSITY, MEDICAL COLLEGE - Sapporo Hygenic Laboratory Dr. Zenjuro Inouye, Prof, of Hygiene Dr. Inouye has been primarily interested in the physiolog- ical effects of air ions and has devoted much of the past ten years to studying various phases of this problem. He has also been interested in the factors influencing the efficiency of the production of immunity by BCG Vaccine in guinea pigs. Some of his observations include the following: A. That the efficiency of BCG in protecting guinea pigs against human tubercle bacilli subsequently innoculated, is re- duced in the presence of: 1* Low temperature 2. High temperature 3 m Low atmospheric pressure Um Malnutrition 5# Low calcium intake 6. Vitamin Bl deficiency 7* Vitamin C deficiency 8* Fatigue 9« Acidosis B. That the efficiency of BCG is maintained or increased bys 1. Administration of Vitamin Bl. 2. Exposure to an increased concentration of air ions for short periods daily. C. That exposing factory workers to an increased concen- tration of air ions for short periods daily increased their efficiency and enhanced their recovery from fatigue. A list of titles of research publications from the Hygienic Laboratory during the past five years is attached. A complete set of reprints has been furnished by Dr. Inouye and is being forwarded under separate cover. HOKKAIDO IMPERIAL UNIVERSITY, MEDICAL COLLEGE Clinical Division Surgical Dept*, Prof. S. Yanaji A list of investigations is attached* Psychiatry Dept* - Prof. T. Ishibashi Some research work was done during the war on the Aborigine Ainu, who are susceptable to the peculiar hysterias of primitive peoples* A list of publications is appended* Pediatrics Dept. - Dr. K. Nagi A list of publications is appended. Medicine Dept. - Dr. S. Nakagawa Experiment on the effect of cold on the bodies of man and animals, both in health and disease, have been conducted for the past 15 years without significant findings. Serological test and chemical test for malignacy have been investigated for several years. Also, test on liver function have been done. A German drug, Benzyliaidazolin, developed in 1941, a vogue stimulator, has been tested* List of publication also attached. Opthalmology Dept. - Dr. Sadarai Ochi Principal work has been done on histopathologic studies of tracoma. Staining technique for inclusion bodies has been de- veloped here. Procedure is described in the accompanying reprints HOT SPRINGS RESEARCH INSTITUTE HOKKAIDO IMPERIAL UNIVERSITY Dr* S, Saeto, Medical Director Experimental work was done on the effect of mineral waters on wound healing and also on intra-arterial injection of steril- ized mineral water on diseases such as arthritis, and on acute and chronic infections* Good results were claimed. Reprints will be forwarded under separate cover* HOKKAIDO DffiERIAL UNIVERSITY, IfdDICAL COLLEGE - SAPPORO ?! thology Dept, Dr. I. Aon, Prof, E crltus D: . K. T he da, P;of, 1st P thology Department D;.‘, H, A" bo, Prof, 2nd P thology D. partnont Dr, IC. S.tnpo, A a-si st ant Dp, K, 0 ata, A sistant Attached are reports of research projects undertaken by the two departments of pathology at Kalchaido I aerial U ivarsity. T'x high altitude studies will be reported in detail under s a parato c over, Tissues and abstracts of research projects and routine auto- psies will bo forwarded under separate cover,. HOKKAIDO IMPERIAL UNIVERSITY - SAPPORO Medical Department Prof. Biochemistry Morio Yasuda, M.D., Ph. D, (Came to Sapporo in 19A2) Investigated metabolic and nutritional studies in connect- ion with the Physical Culture Institute, Tokyo (Now closed)* Investigated the structure of phorpholipids, particularly plasmalogen and amino acid kephalin derivatives. Recent re- prints attached. A summary of more recent studies of the structure of phos- pholipids as well as nutritional and metabolic studies of in- habitants of cold regions as attached. XVII pOKKAIDO IMPERIAL DIVERSITY T, Sapporo Veterinary Faculty Dept, of Agriculture Applied Mycology Cryological Institute HOKKAIDO IMPERIAL UNIVERSITY - SAPPORO, JAPAN Veterinary Faculty Prof* K. Ichikawa - Chief of Veterinary Medicine Dr* Ichikawa stated that Dr* Ono in Manchuria has developed a method of collecting Babesia parasites from blood. It is a sedimentation technic using calcium citrate (10%) solution* The infected cells rise to the top, are pipetted off and sedimented 2 or 3 more times. Dr. Ichikawa said that it might be utilized in the concentration of malaria parasites. Dr. R. Kurosawa - Prof, of Obstetrical Veterinary Medicine Has done considerable work on the diagnosis of pregnancy in the horse. Published papers are being sent in under separate cover. Dr. S. Hamada - Bacteriologist in Veterinary Medicine Has done considerable work on Salmonella infectious produc- ing abortion in the Mare. Published papers are being forwarded under separate cover. He stated that a few years ago there was a considerable amount of abortion in cattle produced by Trichomonas, but upon treating cows with washings of Mercurochrome and Mercuric chlor- ide, or a 0.5% solution of Lysol, this was prevented. Dr. C. Kohanawa, Hematologist Dr. Kohanawa has done considerable work on infectous anemia in horses. He has also done hematological work on rabbits, cows and other animals. Attached is a list of publications. Reprints are being for- warded under separate cover. HOKKAIDO IMPERIAL UNIVERSITY - SAPPORO Department of Agriculture Prof. Agricultural Chemistry, Eigi Takahashi Assft Prof. Nulo Ito Investigations on nutrition of animals. No research was done during the war. Prof. Biochemistry, Yukihiko Nakamura Chemical and physical chemical studies of starches. Work published prior to 194-0• Department of Science Prof* Organic Chemistry, Harusada Suginome Isolated ’•Kobusin", a new alkaloid from aconite. A summary of more recent study of the structure of aconite alkaloids is attached HOICCAIDO IMPERIAL UNIVERSITY - SAPPORO, JA'PAN Faculty of Agriculture; Dr, Jun Hanzawa - Prof, Applied Mycology (Retired) Dr. Y, Sasalci - Prof, Applied Mycology Dr, Hanzawa,retired Prof, of Applied Mycology, after 39 years on the faculty, has worked mainly in the application of microorganisms to agriculture, industrial fermentations, and the preparation of fermented foods, (1) A fermented soybean food iTNatto,? widely used in Japan, is the result of his research, (2) The application of the flax retting principle (pectinase breakdown by microorganisms) to mulberry bark (waste from silk industry) to cotton plant stalk and to Manchurian Phibiscus in the preparation of a strong durable fiber for gunny sack (burlap) production, is an outgrowth of his research, (3) During the war, glucose has been derived from potato starch for use in the work in the laboratory, i'Z.) Am in most other laboratories work on the isolation of Per id.I Mum uotatum and the preparation of Penicillin has been and is in progress. Attempted isolation of other molds with antibiotic capacities is underway, The laboratory at the University is old and unkempt. As in most institutions reagents arc lacking and the knowledge of research being done in the states is meager. Requests for in- formation on Penicillin production arc continually sought. Dr. Y. Sasaki is now the Prof, of Applied mycology carry- ing on the work reported above. He is a former student of Dr, Hanzawa, 'His present interest is in the classification of the mycoderms. HOKKAIDO IIIPNRIAL UNIVERSITY Cryological I-'stitutc Prof* N* N hanura, M-.dical Director Lot; temperature chambers capable- of producing-2$°c and-40°o temperature arc in use. Considerable emphasis was placed upon the studies begun in 1943 to alleviate frostbite* No practical applications were developed* R rid freezing of neat . nd vegetables is being studied at present, D /bailed abstracts and related reports will be submitted under s c par at c c o ve r.. A listof research projects undertaken during tho past 2 7 cars is attached. MINISTRY OI1 HEALTH .AND SOCIAL APY..JRS - TOPYO Bureau of Hygiene and Sanitation The following officials of the Bureau of Hygiene and Sani t at ion war c ini cewed; Dr, Ka.seyoshi Yanaguchi,.Division for Prevention Dr, Taizo Ashida of Chronic Diseases Dr, Yushichi rainamizaki,,,,,,,Chief of the Division for Prevention of Communis able Diseases Dr. Yukiharu Miki ..Chief of the Division of Health The Bureau of Hygiene- and Sanitation according to these officials is an administrative organisation whose relationship to research is one of cooperation and application. Thus, for research, the Division for Prevention of Chronic Diseases de- pends largely on the Tuberculosis Research Institute under Dr, H. Oka (reported separately); the Division for Prevention of Common icable Diseases depends on the Government Institute for Infectious Diseases (reported separately) and the Division of Health is chiefly concerned with problems of nutrition which arc investigated by the National Nutrition Institute, A list of projects which were under investigation in this Institute have been reviewed by the committee chemist, who has indicated no further action is necessary. XIX NATIONAL RESEARCH COUNCIL OF JAPAN Projects Reports on Projects NATIONAL RESEARCH COUNCIL OF JAPAN Dr. Harue Hayashl - Director The Medical Section was supervised by Dr. Y. Miyagawa Funds are granted by the Bureau of Education to the National Research Council which receives and passes upon applications for financial support from investigators. Average yearly grants amount to IS million yen of which 10 million was devoted to war work. Approximately 4- million yen went to medical and related , research. Completed reports up to 194-3 are published in journals of the council with the exception of those investigations carried out for the Army and Navy. Results of work for the Army and Navy were not made known to the council. A list of projects relating to medicine is attached. Investigation of these projects is under way. SUBJECTS RESEARCH AIM NAME OF CHIEF RESEARCHER Flight medicine ¥ 260,000 Study of high altitude fly- ing and diving for purpose of trying to increase abil- ity to withstand these and to prevent dangers. &ato, Toyojiro and 70 others. Tohoku Imp U Underwater medicine ¥100,000 Study to improve living con- ditions in submarine and to increase operating effic- iency. Hisano, Nei and 15 others Nagoya Imp U Ability to stand heat and acclima- tization. ¥110,000 Study to promote working efficiency and to direct way of living by examining individual differences in ability to stand heat and suitability for life in tropics. Hisano, Nei and 17 others Acclimatization to low temperature. ¥120,000 Study on method of protect- ion against cold, precaut- ions against frontbit and further to find a method of increasing ability to stand cold. Toda, Masazo and 8 others. Kyoto Imp U Nutrition in the tropics and in cold regions. ¥45,000 Study on selection of food stuff suitable for climate and on method of increasing production and of storage. Toda, Masazo and 15 others Kyoto Imp U Physical power ¥30,000 Study on maintenance and pro- motion of physical power of people and on individualfs suitability to various man- ual work. Uramoto, Masa- zaburo and 30 others Giohukai Med Sch Sight strengthen- ing. ¥54,000 Prevention of nearsightedness and strengthening of eight in daylight and night. Shioji loshiji and 15 others Tokyo Imp U The health of in- fants ¥50,000 Study on nutrition of infants in wartime, prevention of sickness, motherly care, me- thod of keeping health of work- ing women. Kuriyama, Shig- enobu and 24 others. Tokyo Imp U Blood transfusion substitute, ¥180,000 1 Study of blood transfusion substitute medicine, method of storing of blood, method of blood transfusion. Nakazumi, Masa- toku and 12 others Tokyo Imp U SUBJECTS RESEARCH AIM NAME OF CHIEF RESEARCHER Physical constitut- ions ¥20,000 To study ability & change in ability to resist var- ious diseases from stand- point of constitution & type of body. Koiko, KyiJi & 23 others Chiba Med Col Hot Springs ¥38,000 To study effect of natural hot spring & to apply this in recovery from fatigue. Misowa, Keigl & 11 others Tokyo Imp U Hormone Vitamins ¥60,000 Study of mechanism of action and method of use of (vitamin hormone). Shimizu, Tayoi & 17 others Okayama Med Col Infectious cerebro- spinal meningitis ¥50,000 Various chemotherapeutic methods parallel use of in- noculation serum, measures against carriers, & improv- ment of vaccine. Miyakawa, Yonoji and 5 others Tokyo Imp U Digitalis ¥27,000 Submitted on report from 22 Sept thru 8 Oct 45 Kobayashi and Ichidate Tokyo Imp U Prevention of malaria and treatment ¥60,000 Prevention of malaria, malarial mosquitoes, san- itary installations, treat- ment test of efficacy of new medicines. Hayashi, Harno Preparation of anti- malarials ¥60,000 Synthesis of new anti-Mal- arial compounds. Asahina, Haruhikc Prevention of dengue fever and treatment ¥50,000 Culture of dengue parasite, animal innoculation method of diagnosis, experiment with human being. Keizumi, Tan Leprosy ¥50,000 Animal innoculation, cul- ture of bacteria, now chem- othera phy. Ota, Masao Breeding of small animals for experi- ments, ¥25*000 Finding best method for quickly increasing number of small animals for ex- periments. Okada Kanarae & 9 others Tokyo Imp U Regulating mechanism in living body & its military application ¥4.0,000 Aim to create new type of equipment Aida, Tokusuke and 8 others Tokyo Imp U SUBJECTS RESEARCH AIM NAME OF CHIEF RESEARCHER Emission Ray ¥120,000 V Study on method of indirect X-ray photography, on its application to group exam- ination for tuberculosis & on effects of various emission rays on living matters. Nakazumi, Masa- toku and 12 other Tokyo Imp U Tuberculosis ¥140,000 Study on Innoculation against tuberculosis, im- provement in method of treatment, and on problem of work for tuberculosis patients. Imanure, Arao & 63 others. Osaka Imp U Filterable viruses ¥20,000 Study of influenza, small pox, trachoma Takeuchi, Matsu- jiro and 21 other Osaka Imp U Immunity ¥50,000 Study on innoculations against various infectious diseases, mass production of innoculation serums, methods of storage & use. Tamiya, Takeo & 28 others. Tokyo Imp U Fatigue ¥94,000 Study of nature, diagnos- tic method, prevention of & recovery from fatigue. Katsunuma, Seizo and 36 others Nagoya Imp U Nutrition effic- iency ¥78,000 Study on nutritional value of wartime food in Japan, Korea, Manchuria, & deter- mination of minimum amount required for different pro- fessions Toda, Masazo & 13 others. Kyoto Imp U Eruptive typhus ¥96,000 Study on innoculation against diagnosis extermination of er- uptive typhus & also on prob- lems concerning mass living sanitation. Miyakawa, Yoneji and 20 others Tokyo Imp U Brain Waves f38,000 Study on picturization of brain wave & its application Katsunuma, Seizo and 11 others Tokyo Imp U Enzymes ¥40,000 Chemical research on various enzymes k quanity production of those which can be used to increase physical power & heat standing ability. Kobu, Yashiro and 16 others Osaka Imp U Physical Standards for Japanese *40,000 Determination of physical standards by measuring young and old male Japanese, Nishi, Naruko & 24 others Tokyo Imp U SUBJECTS RESEARCH AIM NAME OF CHIEF RESEARCHER Insects, ¥35,000 To make clear the cycle of diseases such as mal- aria and eruptive typhus which are carried by in- sects & to study prevent- ion of these diseases. Bokuzawa, Sanji and 13 others Tohoku Imp U Military horses & animals ¥80,000 Study on increasing prod- uction of military horses & other animals for military use & on their sanitation so that source of military horses in wartime can be maintained. Emoto, Osarau and 14 others Tokyo Imp U Domestic animals & domestic fowls ¥44,000 To plan to increase number or best domestic animals & fowls by applying principles of heredity in present sit- uation of insufficient feed. Masui, Kiyoshi and 11 others Tokyo Imp U Parasites of domestics animals To exterminate harmful para sites of domestic animals, to promote efficiency of mili- tary horses & general domestic animals. Yoshimura, Ichiro & 5 others. Vet College Wartime food for health. ¥89,000 Treating of food stuff; rationalization of combin ation & cooking; preparing food from things not used as food but which are edible, making inedible substances edible; grains powdered food; country food stuffs; search & use of natural vitamin sources; effects of vitamin & others in a limited food supply. Yabuta, Teijiro & 28 others Tokyo Imp U Electronic micro- scope. ¥30,000 To study to improve elect- ronic microscope itself & to give basic assistance in solving various problems which require early solution. Seto, Shozo & 8 others Tokyo Imp U NATIONAL RESEARCH COPNCIL OF JAPAN Reports on Projects The following NRC projects have been turned over to other sections, as indicated, for investigation; since the subject in- dicated that the project would be of primary interest to that section. 1. Wartime Water Works and Sewage - To Office of Chief Engineer - Major Block. 2, Flight Medicine - TO ATIG, FEAF - Capt. Castor 3. Underwater Medicine - TO NAVTECHJAP - Comdr. Ayres The following projects have been reviewed by the committee and it is considered that no further action is required. Many have been reported upon under the institute where the work was done. 1. Studies on the regulatory function in the animal body. 2. Factors influencing the growth and fermentation of microbes. 3. Nutrition Projects. 4. X-Ray indirect radiography. 5* Electro-encephalograms. 6. Balneotherapy 7. Studies on Vitamins. 5. Substitution Method for Blood Transfusion. 9. Studies on Epidemic Cerehrosfinol Meningitis. 10. Studies on Epidemic Tophus. v 11. The metabolism and the action of Vitamins under hot temperature • 12. Section for the preparation of Anti-Malarial Remedies. 13* Chemotherapy of Tuberculosis. 14. Malnutrition. 15. Electron-Miscroscope 16. Studies on Architectural Hygiene and Equipment. 17. X-rays. 18. Projects relating to Physical Strength. Attached are reports from the Japanese on the above subject. XXjfcKAKAZAV/A GOVERNMENT MEDICAL COLLEGE Biochenistry Department Internal Medicine Dr, T. Tani, Director of Bacteriological Institute Department of Pharmacy Dept, of Pharmacology Pharmacology (Dr, H, Okaraoto) GOVERNMENT MEDICAL COLLEGE, Kanazawa Biochemistry Department Prof. Ken Iwasaki Investigated and developed gasometric methods for chemical analysis. Has particularly studied and developed methods for determination of urea, uric acid, creatinine, guanidine, etc. in blood. Has developed a gas measuring apparatus, in which 10 micro- grams of Nitrogen can be determined, forwarded under separate cover. He also studied methods for the determination of Iodine. The laboratory was very well equipped and organized and the reviewer believes the quality of work to be good. Attached are a list and reprints of the work of the depart- ment. GOVERNMENT MEDICAL COLLEGE. Kanazawa Dr. M. Suzuki, Prof* of Intenal Medicine Considerable work has been done by Dr. Suzuki on chemotherapy in tuberculosis. A study is now in progress on the treatment of tuberculosis with oaminophenal. Of sixty patients treated, 10$ showed a disappearance of the organism from sputa, urine, or gastric washings, and on x-ray examination, healing of the lesions This is a preliminary report. No ill effects were reported in any of the patients up to this time. Reprints of the work on tuberculosis will be forwarded under separate cover. Reference is made to report on the Pharmacology department of this college. GOVERNMENT MEDICAL COLLEGE, Kanazawa Dr. T, Tani, Director of Bacteriological Institute The main interest of the institute has been in spirochaetal studies. Efforts are being directed principally in an effort to culture T. pallidum in-vitro and to prepare a vaccine. Seventy-four reprints of the work in this Institute have been obtained. A translated listing of this work from 1940-194-5 is attached. These articles are published in the faculty organ. GOVERNMENT MEDICAL COLLEGE. Kanaza Department of Pharmacy- Prof, Yosio Sakurai Investigated certain alkaloids and found one (Nupharidin) useful as a local anesthetic* Attached are 2 reprints and a list of publications with titles which were published in the Tokyo Journal of Japanese Pharmaceutical Society (reprints were not available)* GOVERNMENT BEDICAL COLLEGE, Kanazawa Dr. H, Okamoto, Prof, of Pharmacology A product o-aminophenol vitro and in-vivo (animal in tuberculosis. The attached self explanatory is being used after in- tests) - in human therapy digest of this work is Samples of o-aminophenol and another product, 3-aminophen- oxazon - (2) have been procurred. Also an ampule of the o-amino- phenol in solution, as used in therapy, will be forwarded under separate cover. Reprints on the work on o-aminophenol and on Prof, Okamoto *s work on streptolysin will be forwarded under separate cover. GOVERNMENT MEDICAL COLLEGE. Kanazawa Dept, of Pharmacology Directors Dr. N. Ishizaka Investigated and. synthesized new compounds for chemotherapy of pneumonia. Developed a compound (Decasulphone) which is claimed to be more effective against streptococcus pneumonia. Reprints attached, samples (2) under separate cover. Tested numerous alkaloids and new synthetic compounds for treatment of bird malaria. Dime thozy-8-die thylaminocthylamine- quinoline was found the most satisfactory (sample under separate cover). A list of compounds and summary of tests conducted are attached. A list of papers from the department, with titles and re- prints are attached. XXI" KTOEO HvSPERIAL UNIVERSITYt MhDICAL COLLEGE "Leprosy Institute . IMlcrobioleigy-. (_Dr, R • ICimura) Pharmac eut i ca1 D epartno nt Department of Pharmacology Research Projects Tropical Diseases': Utizino-Laboratorinm for Chemical Research KYOTO IMPERIAL UNIVERSITY. MEDICAL COLLEGE Dr, R. Kimura, Microbiologist and Dean of School Dr. Kimura is the pioneer in electronic microscopy in Japan, using an instrument which he constructed. His photographs of micro organisms are the equal of any done in the U,S,A,(Mudd)• His most pertinent observations in this field are on the intracellular granular material of the paratyphoids - other gram negatives and on C, dinhtheriae. The exact nature and importance of the bodies brought to light by the electronic microscope has not yet been observed elsewhere and is in corroboration with American and German work. The other studies by Dr, Kimura include the following: Fowl pest - The development of a formolinized vaccine (virus) giving good immunity. Experimental inoculation of dogs with bovine and human T.B.- Dog is resistant to former but susceptible to the latter. The dog, as a possible carrier, is indicated. Differentiation of human and bovine T.B, - by chick embryo inoculation. Embryo more susceptible to the bovine strains. Dengue fever - Cultivation of virus on chick embryo and in mouse brain, A mouse brain vaccine showing good protection in animals prepared. Endemic typhus - Attempted animal infection intranasally, in open wounds, and on the conjuctive. Resultant infection by any of these routes successfully done. Two unpublished manuscripts on endemic typhus were picked up by Lt, Col, Sanders, CWS# Further data on this work could not be obtained. Bangs disease (Undulant fever) - Serum of dairy workers (Kyoto showed 2 of 116 men with agglutinin titer 1-100. Of 29 cows tested, eleven gave positive reactions (reactors). Analysis of toxin by peptic and other enzyme digestion, A study on the nature of toxin, (Chemical) The incidence of M, tetragenous in upper respiratory infect- ions. Found to be a common secondary invader. Immunological Properties of Cultured Lelshmania donovani Four rabbits were inoculated with killed (by heat) leptomanas forms of L, donovani. In the serum of these rabbits were found agglutinating substances, precipitins, complement-fixing anti- bodies and anaphylactic antibodies. All of these various react- ions were said to be strain specific - could tell the difference between 2 strains of L, donovani. Skin Reactions for the Diagnosis of Kala-Azar. Antigen prepared from cultivated leptomonas forms. Injected 0,1 cc intradermally, Tested 16 Japanese soldiers infected with L, donovani and obtained positive results in all. The height of the reaction was from 12 to 2A hours. Some positive reactions were as much as 55 MM in diameter. Four controls were negative. About 30 cases of leishmaniasis in Japanese soldiers in Kyoto Hospital and many more in Hiraeji Hospital, near Kobe, All soldiers were from North China, Chemotherapy of Kala-Azar with Five Antimony Preparations, He found that all five drugs would cure striped squirrels infected with L. donovani. In vitro and toxicity studies were conducted. These drugs were also injected into a normal animal and then an emulsion of livers infected with L. donovani was in- oculated intraperitoneally. All drugs prevented the squirrels from acquiring leishmaniasis. All controls contracted the dis- ease. Injection of L. Donovani into Rabbit Tisticles. Dr, Kimura injected leishmania and leptomas forms into the testicles of rabbits. In some animals the leishmania could be found in the liver, spleen and bone marrow but could always be found in the testicle. Reprints of the above publications are attached. KYOTO IMPERIAL UNIVERSITY Pharmaceutical Department Torlso Takahashi Synthesized numerous sulfur-pyridine containing compounds, list of compounds, as well as reprints are attached# KYOTO IMPERIAL UNIVERSITY Department of Pharmacology Prof. Kikuo OgiU Ass*t. Prof. H. Yamazoki Takeka Seki, Assistant Investigated many compounds (list attached) for treatment of bird malaria. All were found less effective than atabrine with the exception of 2 methyl-mercapto 6 chlor - 9 - (diethylamino pentylamino) pyridine 3*2* s 2:3 quinoline chlor hydrate which was better than atabrine. Method of preparation attached, sample under separate cover. Investigate an antimony compound (antimonyl hexonate) pre- pared by Prof. Nakai at the National Chemical Institute, Tskatsuki. It was found very effective in treatment of 10 human cases of Kala-azar (reprints attached) and has also been recently found of decided value in 30 human cases of Schistosomiasis. The com- pound is relatively non toxic, given in a course of 25-50 intra- venous injections of 0.5 - 0.6 gn* Lethal dose for 10 gm. mouse is 40 mg. Investigated certain antipyretics and one was synthesized with properties similar to morphine. 3 reprints attached. Attached is a list of 63 publications with attached reprints. KYOTO IMPERIAL UNIVERSITY. MEDICAL COLLEGE Research Projects Attached is the report of the research projects in medicine that have been pursued by the members of the staff since 194-0* Reprints of the articles which appeared to be of most interest are being forwarded under separate cover. Specific reports on most pertinent projects are submitted under separate title. KYOTO IMPERIAL UNIVERSITY, MEDICAL COLLEGE Tropical Diseases Dr, S, Yamaguti, Prof, in Dept, of Science Dr. Yamaguti was a Captain in the Japanese Navy, From August 194-3 to December 1944-* he was doing research work in the Macassar Navy Institute for Tropical Diseases in Macassar, Celebes, Malaria Most of his work was done on experimental bird malaria. He worked on the theory that a lowered oxygen tension, in the circul- ating blood, might help destroy the malaria parasites. Ten birds infected with a species of malaria ( probably Plasmodium praecox) were first injected with quinine, then put in a glass dossicator and the air evacuated. They were kept at this negative pressure for 30 minutes. The treatment of birds in this manner was found more effective than by the simple administration of quinine. During the past $ years, he has published many papers on the parasites of fish and other animals. Reprints are being sent under separate cover. um imzm Utizino-Laboratorium for Chemical Research Director; Prof, S. Utzino Prof. Utzino, until 1942, was Director of Medical Chemical Institute, Imperial University, Sendai, and came to Kyoto in 1942, Extensive investigations were carried out on digestive proteolytic enzymes. Enzymes from the kidney and liver of whales were recently studied. Attached are 44 reprints covering work done at Sendai and Kyoto since 1940. XXII. KYUSHU I1IHSRIAL ul'TIVERSm: Bacteriology Dopt, Hygiene Dopt, Orthopedic Surgery Pharnac ology Pathology Eept, Public Health De:b* Clinical Division HYU8HU II.AEhX/X uHIVEhEITY Faculty" of IhmicTinc Bactcriolopy Depart! .ent Dr, Tadao Toda, Prof, of Bactcriolopy and Protozoolopy . Doctor Toda and his assistants have carried on r.u extensive research propran durina the war, heprints of publications of the Depart!.ent are beinp forwarded under separate cover, fmonp the probieiis studied arc included the following: 1, EGG- i: nunization - Doctor he da c la ins to be the first to have used EGG iinunization in Japan, Ho is the author of a nonopraph on the vaccine. He is in complete apremmenu until all other investigators in oiaiminp narked reduction of tuberculosis morbidity and mortality amonp immunised individuals. His labor- atory is the production center of EGG for use in ICyv.shu, 2, Leprosy - all a.tteupts at cultivation of or animal infec- tion with human leprosy bacilli failed, 3, Dcnpue - while th . primary objective of Doctor TodaPs studies on dengue was to determine size of the virus, the interest- inp point is that he claims to have prown the virus on the chorio- allantoic membrane of the developin';; chick embryo. A specimen of this virus in dried human blood, preserved in the frozen state since 1943*v;r.s infective bo humans in 1945. A sample of the dried blood has been secured for forwardinp under scparate cover, 4, Chemotherapy of malaria - experimental antimala.rial com- pounds were received from Prof, Takahashi of the Hyoto Imperial university for stud3', hmonp the proup of compounds studied, one was rewarded as havin/; merit: 7 chlor 61 mcthoxy - 4 - ( a dia.thlonino S - pcnty - amino) - { - 3 ’ - 2! = 2 , 3 chinolin) chlorhydrat• 5. Penicillin - an experimental product T/as obtained which ■nas therapeutically effectivu but produced reactions, no oomor- cial production of penicillin was undertaken. ITo investlections of influenza, typhus or encephalitis wore undertaken durinp the war. KYUSHU IMPERIAL UNIVERSITY Faculty of Medicine Hygiene Department Dr, Kiomi Atsubo, Prof, of Hygiene (and Parasitology) Doctor Atsubo has interested himself in climatology but also has sponsored some research on the identification and distribution of helminths. Reprints of publications of the Department of Hygiene are being forwarded under separate cover. KYUSHU IMPERIAL UNIVERSITY Faculty of Medicine S♦ Jirinaka, Dean of the Medical School and Prof, of Orthopedic Surgery "Prosthetic Limb Studies 1* This department was asked early in the war to make re- searches into the problem of limb prosthesis and much work has been done along this line* The whole problem has been reviewed and the mechanics of various types of artificial limbs have been improved* Several new upper limb attachments have been devised to assist the crippled farmer in his occupations. Three papers are appended. 2. Many studies have been conducted on the handling of traumatic amputations and joint injuries. Studies on the case of histamin and choline injected locally to prevent anklyosis and also on the use of fascia lata to prevent joint enkylosis have been made. 3. Much work has been done on the prevention of infection in traumatic joints and in compound fractures. He has developed no new techniques on adjustive procedure. A. The professor has just started a study on the effect of the atomic bomb on bone growth in children. The bone tissue from autopsies is being studied. KYUSHU IMPERIAL UNIVERSITY Faculty of Medicine Dr. Tokushi Fukuda, Prof, of Pharmacology Research was conducted in three phases: 1st Phases Digitalis and digitalis-like substances. Exten- sive studies were made on Neriura Odorum, habitats South Asia, a powdered extract was prepared and experiments conducted in frogs and rabbits. Action is the same as digitalis and found less toxic. Reprint attached. 2nd Phases Pharmacology and Physiology of heart and blood vessels* Study of no significance. Reprint attached. 3rd Phases Toxicological study of Globe fish (Japanese)* A toxic substance known as "Tetradotoxin" was found in liver and ovary of fish and sometimes in the skin* Muscle of fish not poisonous. Reprint attached. ipojsiiu zqaapxal DivrvhiusifY Faculty of hedicine ~ Patholopy Depart:xnt Dr. Tanahi Inai, AssTt. Prof, of Pathology (Substitute for Dr. Aosahu Ono, Prof, of Pathology and Dr, Yuhizo Ohno, Pi nr it us Prof, of Pathology) Dr, Inc A, v/iio v/ill succeed Dr. Yuhiso Olino c.s Professor of PufYoloGY, discussed the v/orl: of the Deourtiient c.s follows: 1. Doctor Ono has spent nany jeers studying tin lynphatic S3rstcr- His studies have been concerned ohicfly nith anatomy and pliysiolopy, tin blood supply of the spleen, the etiology of splenor.e- ,aly and the pathogenesis of tuberculous lyiipiiadenrtis. 2. Doctor Olino lies studied the problem of Jaundice for 20 years. He is responsible for the unitarism theory of icterus which implies that Jaundice does not occur in the absence of liver pathology. 3. I'hc autopsies peri orned on atonic bonb patients { 24 total) arc beiny reported to Colonel Ouphterson,of the Aton Bonb C omission. He prints have reqims'imd for forwarding under separate cover. KYUSHU IMPERIAL UNIVHRSIff; Faculty of Medicine Public Health Department Dr. Harvo Mizusima, Prof, 5f Public Health Doctor Mizusina, v;ho holds a iegree of Doctor of Public Health from Johns Hopkins University, has devoted most of his re- search to population problems in Japan, Korea, Manchuria, and Mongolia, He has reported on population increases in cities and rural areaayand has developed life tables for Japanese, Koreans, and Manchurians, In addition Doctor Mizusima has sponsored re- search in industrial hygiene, Vitamin C content of foods, puri- fication of water by the oligodynamic action of silver, construc- tion of houssiBi for maximum comfort, effects of ultraviolet irrad- iation, effect of high temperature on the development of tuber- culosis, effect or low pressure on the development of tuberculosis bacteria which utilize carbon monoxide and the influence of flou- rine in water on mottling of teeth in Koreans, Reprints of publications are being forwarded uid cr separate cover. III. Dr. T. Mitsuya, Prof, of Urology A large part of the work of this department, together with the apparatus used, was lost in the bombing and burning of the urological laboratory. They have made a study of the normal physiology of the urinary tract using x-ray cinematographic methods. Peristaltic movements of the kidney, pelvis, ureters and bladder were studied. Reprints were requested for forwarding under separate cover. IV. Dr. F. Ishiyama, Prof, of Surgery The professor has the reputation of being one of the best surgeons in Japan. He is carrying on the work of his deceased predecessor. Prof. H, Miyaki, on gall stones. He has collected stones from Japanese living in China, Formosa, Korea and other parts of the world in a attempt to explain the high incidence of bilirubin stones in Japanese. The professor has studied the composition of gall stones, using microscopic, clinical, spectrographic and x-ray disfraction methods. He feels that the figures given in text books and in the literature for the incidence of various types of gall stones will have to be revised in the light of his researches. He feels that all the various analytical methods must be employed to determine the true make up of the calculus. He is now applying his methods to the analysis of urinary stones. His paper on X-ray crystalography is appended. 2. His department has used the Fuch’s, Kurten and Sakai meth ods of diagnosis in early gastric carcinomas. In their hands the tests are accurate and are falsely positive in about 155&. 3. Ishiyama has done over 500 gastric resections for cancer and does not hesitate to take the hepatic artery if he feels it is necessary. He has done this in about 10 cases and has been sucessful in all. They have shown by animal experimentation, that the liver blood supply is readily taken over by the gastro- hepatico-duodenal artery. 4>. He has done 4.0 frontal lobectomies in epileptics with discouraging results. He has resected the bronchial ganglia in 60 cases of asthma with modiocre results. His paper on post operative pulmonary complications is appended. V. Dr. Sigeru Umayahara, Prof, of Obstetrics and Gynecology The Department of Obstetrics and Gynecology has investigated such problems as blood supply of theanbryo, liver function in toxemia of pregnancy, action of various hormones, roentgen pel- vimetry, physical measurements of the newborn, insufflation tests for tubal potency, Vitamin C excretion and sodium tanro- cholate as a treatment for sepsis. Reprints of publications are being forwarded under separate cover. VI, Dr. G. Kusunaki, Prof, of Internal Medicine The department of medicine of this university has an ex- cellent reputation in Japan and is active in experimental and clinical research. 1. Studies have been conducted on the autonomic nervous system using the picrotoxic and section method. By this method a small area in the ventral part of the pons has been shown to be the center for tonic control of the intes- tine and the spinal cord and peripheral paths have been worked out. Reprints are attached. 2. Studies have been and are now being conducted on the effect of gastric and duodenal substance and human saliva and serum on polycythemia and perniceous anemia in the white rat. These stud- ies have not been completed and have not been published. 3. They have shown that the use of gastric and duodenal sub- stance in conjunction with typhoid vaccine causes a marked in- creased in the production of immune bodies and a higher titer is obtained than results from the use of the vaccine alone. A reprint of their paper on this reaction is appended. A. The staff has conducted much experimental and clinical research on the problem of bronchial asthma. Many reprints on this problem are attached. They have used fever therapy and insulin shock therapy with claimed excellent results. VII. Dr. M. Sasaki, Prof, of E-N-T. The professor has published numerous papers on his method of tonsilectomy, on the clinical problem of tonsilitis, and on the removal of foreign bodies in the bronchial tree. Reprints are appended. VIII. T# Takeda, Prof, of Roentgenology- The only study conducted in this department recently has been one on the use of X-Ray therapy in the treatment of leprosy. Good results have been claimed for the maculous form of the dis- ease, The results in the nodular form of the disease are not good. Reprints will be forwarded under separate cover. XXXII II&.GOYA II.IPEItlAL TJYIVEasiTY i Faculty of Iviodloino Anatomy Department Department of Bacteriology Clinical Division Comments on Medical Education Hygiene Department Optlialmology Pathology and Parasitology Dept, of Pediatrics Pharmacology (LIivra) NAGOYA IMPERIAL UNIVERSITY Faculty of Medicine Anatomy Department C. Togarl, Prof, of Histology and Embryology The department has made many investigations in the histol- ogy and histogenesis of various organs in rodents# Similar studies have been conducted on the sweat glands of the human axilla and on the nasal muscous membrane of man. Reprints will be submitted under separate cover. Dr. K. Yamado. Prof, of Anatony (Micro) The professor has been interested in the variations and anatomical abnormalities of trunk muscles of man, particularly of the levator scapulae. He has made exhaustive studies. Reprints will be submitted under separate cover. Dr. H. Nagamatsu, Prof, of Anatoner (gross) The professor is conducting, in conjunction with all Jap- anese anatomists, a comprehensive study of the Japanese body types. This work has not been published. NAGOYA IMPERIAL UNIVERSITY Faculty of Medicine Department of Bacteriology Dr. Kazuo Ogasawara, Ass't. Prof, of Bacteriology (substitute for Dr. Mitsuzo Tsurumi, Prof, of Bacteriology). During the war the Department of Bacteriology concerned itself with studies of the influenza virus* English, American and Japan- ese strains available for study were all type A, The few sporadic outbreaks of influenza in Japan during the war were identified by neutralization tests with patients sera as also due to type A virus. Experimentally, four types of influenza vaccine were prepared: 1. Emulsion of infected mouse lung tissue. 2. Tissue culture 3. "Purified" suspensions of virus from mouse lung tissue. 4. Chick embryo preparations. The first of these caused severe reactions in humans; the second was less drastic in its effect; the third was most accept- able to recipients. None was produced on a large scale and none was properly evaluated because there were no extensive outbreaks of the disease. In addition to the above, studies were made of the feasibil- ity of using the Manchurian hamster (Citellus mongolicus) as an experimental animal for Japanese encephalitis virus. It was found that the virus survived on 3-4- intracranial passages. According to Dr. Ogasawara, no Japanese encephalitis virus vaccine was produced in Japan for control purposes. The entire bacteriological laboratory and all equipment, data and cultures were destroyed during the first bombing attach on Nagoya. NAGOYA IMPERIAL UNIVERSITY Faculty of Medicine Clinical Division I. Dr, U. Kikkawa, Prof, of Obstetrics and gynocology This is a busy clinical department and they have been con- ducting several clinical and experimental studies in the last 5 years: 1, A study of tuberculosis in pregnancy, 2, A clinical investigation of malignant chorionepitheloma, 3, An extensive study of foetal bone ossification, 4-, A study of placenta praevia, particularly its X-ray diag- nosis, $, The use of laparoscopy in AZ tests, 6, A study of so-called foetal respirations by roentgenograph- ic methods. Reprints will be forwarded under separate cover. II. Dr. M. Akune, Prof. , Oto-rhino-lar The experimental investigations of this department have been concerned with the physiological effects of industrial noises and of total body vibrations of high frequency: 1, The effect of noise on the Bi content of the livers of rabbits, 2, Histological changes in the organ of Corti of white mice subjected to factory (airplane riveting) noises, 3, A study of the effect of industrial noises on factory workers, 4, The effect of high frequency total body vibrations on the utrical sac of animals. Reprints will be forwarded under separate cover. Ill, A. Katsumuna, Prof, of Internal Medicine and Director of the Hospital, The professor is the leading figure at the school and hospital and is interested in hematology. 1. He devised the sterual puncture apparatus used by the Army and Navy. 2. He has made a study of the histology of marrow tissue in lead poisoning. 3* He has devised a method of extracting cattle bones and used the extract in the treatment of emaciation due to starva- tion. A. He has written a very interesting statistical paper on the incidence of the various types of brain tumor in Japan, ill. continued; showing the relative infrequence of gliomas and the high in- cidence of parasitic cysts and tuberculosis* 5, Recently he has been working on the problem of fatigue in factory workers and has developed several fatigue tests. Reprints will be submitted under separate cover. S. Okada, Prof, of Internal Medicine The professor has made extracts of various carcinomatous lesions and used them in the treatment of cancer. He also has an extract from inflamed gall bladders which he used in the treatment of gall bladder disease. Reprints will be forwarded under separate cover. Oral Insulin Dr. Okada has prepared insulin for oral administration as follows: Bosin 0.01 gm, Acid pigment Methyl Violet 0,01 gm. Alkaline pigment Saporin 0,1 gm. Insulin - as required Insulin given in this manner requires three times the quan- tity of insulin as when administered hypodermically. Acid pigment prevents action of Pepsin Alkaline pigment prevents action of Trypsin Insulin prepared this way is absorbed by intestines Clinical study was made on 50 cases. IV, Dr. W. Saito, Prof, of Surgery The professor is one of the best known surgeons in Japan and has been very busy during the war with his practice and run- ning the surgical wards at the hospital. He has done clinical research in spinal anesthesia and has directed a study of the oesophagus by roentgen kymography and tomagraphy. Reprints will be submitted under separate cover. S, Kirihara, Prof, of Surgery The professor is head of the 2nd Surgical Service at the university hospital. He has done considerable experimental work in nerve suture and on surgical shock. He has also done consid erable gastroscopy and has devised an improvement of the Wolf shlndler flexible scope. Reprints forwarded under separate cover. V# U, Supitn> Prof, of foychintry Hr.s :-r.de an extensive stud"' of the character and personal- ities of ur.r factory uorhers and find that 20p of tin:. are abnor- mal. he beliVwS that poor leadership of factory forenon is respo siblc for :iost of the variations* lie lino nlso consiC.err.blc ’..or!: i.-itli the use of the clcctrocncephnloprnn In various psychosis. iiOlu c. . >_inc*j Oil roee„iu v/cr« Other reprints Mill be forwarded under separate cover. VI. 3, Uapura, Prof. of Orthopaedic Surgery fne processor is v/r r Id Idiom for Ins studies of congenital dislocation of the hip '.id. has seen ever 1500 or.ses in’ his clinic in the Ir.st 20 yenrs. lie hns continued hn.<5 studies durin.;, the- nnr. 0.1cprints will be fojr..‘circled toder coVvr. T.LGrOYL IITIIEIYL Faculty 6Y I.cYicYYc*' Dr, H, Tamura, Prof, Dcrmatolocy and Doan of the Medical School. wCoinonts on Medical Eclucr.tion'1 The doctor*s v;ork lias been confined to ach ministration of the medical faculty and school, The medical colicye graduated the same ninber of doctors duriny the mar as before (400 ner doss) but the curiculun \/as shorteiiod fron 4 to 3 years. Thv.ro r.iu (according to Dr, Tenure.) 70,000 doctors in Canon and only about 20,000 of then ore graduateo of t!ic full 4 year-' or 4'l year courses, Eo does not del these non ore nr on or ly trained and hones nany of then v.ill return for graduate* instruc- tion. Like other Jcipcaic sc Cock ore v;ith v/hon this natter ho s hv.cn discussed t Tanuro * s nain concern is that the profess ion is over crowded. M.QOTA Faculty of Medicine Hygiene Dept* Dr* Bogo Koinuina, Professor of hygiene During the war Dr* Koinuma studied the problem of industrial dusts* He tested a variety of face masks and decided that a mask consisting of fine layers of "habutal" grade silk was most feas- ible for production in Japan* This mask, however, did not come into commercial production* In addition to silicosis occuring in coal and copper miners, foundry workers and sand blasters. Dr, Koinuma also listed derma- titis, carbon monoxide poisoning and pneumoconiosis in people grinding duraluminum as common industrial health problems* In addition, tuberculosis was reportedly common in the last named - group. According to Dr* Koinuma, the protective creams used as prev- entives against dermatitis were of the cosmetic type and non specific in their effect. In addition, Japanese laborers were reluctant to wear proper respirators as a protection against dusts. Ventilation equipment to remove both dusts and other atmospheric contaminants were limited in use because of a lack of material* NAGOYA IMPERIAL UNIVERSITY« MEDICAL SCHOOL Dr* M. Nakashima, Prof* Opthamology Dr, R, Hagino, Ass*t Prof, Opthamology Work was accomplished on the use of Posterior Lobe of Anterior Pituitary Gland, where a Melanophalen Hormone was extracted and used in night blindness. This hormone was extracted from fish and prepared by Aoyama Institute, Tokyo (Commercial Pharmaceutical bourse), Dose: 1 cc, became effective after 2 hours and duration was about 24 hours. Results: Night vision was improved, especially objects on the horizon. Fish glands were used only because of the supply* Note: Supplent to Appendix "A", Section JX, Navy Medical College, Tokyo - Dept, of Pharmacy. NAQOYA IMPERIAL UNIVERSITY Faculty of Medicine Pathology and Parasitology Dr, Pukuzo Oshima, Prof, of Pathology and Parasitology Doctor Oshima has devoted many years to the study of the distribution, pathology and epidemiology of clonorchis sinensis, metagonimus yokagawai and Dibothriocenhalus latum infections. All three parasites are endemic in the prefectures surrounding Nagoya due to the native custom of eating raw or partially cooked fish. Since there are no satisfactory therapeutic measures, the only practical procedure at present is to educate the people to cook fish before eating. A reprint outlining some of this work is attached. During the war Dr, Oshima also studies the pathology in sol- diers who died of malaria. Reprints of this work are attached. Before the war Dr, Oshima was studying carcinomas but was forced to discontinue the work due to lack of supplies. At pres- ent he is interested in the etiology and transmission of chicken sarcoma. NAGOYA IMPERIAL UNIVERSITY Faculty of Medicine Dept, of Pediatrics Dr. Kyoichi Nakae, Assft. Prof, of Pediatrics (Substitute for Dr, Okira Sakamoto, Prof, of Pediatrics) "KKIRI" During the war the Department of Pediatrics was largely con- cerned with the study of "skirl” (diarrhea in young children). The studies conducted by the staff show that the disease is prev- alent in children between the ages of two and seven. Untreated patients have a mortality rate. Death appears to be due to cardiovascular collapse. Experimental observations seemed to show that intravenous saline and glucose combined with the administra- tion of strophanthin was the therapeutic procedure of choice. The disease apparently is due to a variety of bacterial eti- ologic agents. A reprint is attached which describes the experi- mental observations made including etiology and therapy. i'AG-OTA EZddZddL IddCAAAdCdY - I.APICAL dCZOOL Dr. TlalcoYo 1 3rfa,~ rr * lie seared carried on over oeriod 1940 to 1945 1. Action o:C Adrenulin on the secretion of the storr.cli. Conclusion: Adrenalin does not dire Voly increi.se secretion of tlic sto: .acd, but rtlaulates activity' of tlie stoi a cli, died in turn st I: mlr t cs seeretions, 2, hi 1 ccts oi o:177011 r.nd r.ir on move:saint of intestines* Conclusion: Vfdcn oizz.on content is vednoed to r. narked decrease is noted in nov#eiibnt of intestines, iron. 20,j to 9A re duct ion,# iTo edanao is noted. 3, Affect'oi acid on secretion of sue atonal a.lands, Conclusion: Injection of' 5 cc, !;■> I c.'l, sIi.ov.ccl an increase in secretion. 4# Action oi caffeine as a lie art ,rbi:- aidant ♦ Conclusion: Caffeine stl ulatcs lie ai> bz direct stir .illa- tion and not bz rnejeao Ir.G oil dilation of blood, Tliic i.as jro/cn b*Z Irecaino; blood pressure and rate of Giro til at ion constant. Blood pressure v:ac lie Id constant bz i.itddrav- ina blood as circulation :dicr eased. Circula- tion of blood aas lie Id coiistrnt If uitddrauina blood as blood n, C ku j ire .Increased, XXIV. OICAYAIIA UNIV32RSITY, Faculty of Medicine Anatomy Department Bacteriology Department Hygiene Department Int erna1 Medi c ine Pharmacology Department Pathology Physiology Biochemistry QlufYAI UlVIYAidlfY faculty bf' Lb die Inc Imat oi. y D e parti lent Dr, llasaji Prof, of Anatory and President of the Japanese Anatomical Gocioty. Doctor Sel:i and his staff have concerned themselves primarily viith tho histological chances due to hot springs therapy. Avail- able reprints detailing tlmsv. and otlmr studies for- \.arded unfAr sc ear at cover. All buildings and practically all equipment and public ati ons of the Do jartiiwit of Anatoiy vcro destroyed durina on air rr.id• QlC/TfJIU ITT Faculty of 1 _Jdic inc Bacterioloyy Department Dr. Sakae I uraharJL, f.ss't, Prof, of Bacteriology (Substitute for Dr,. II. Suzuki, Prof, of Bacteriology) The Department of Bacteriology, uhich also teaches parasit- oloyy, has investiyated four :.a.]or problems Curiae the nor. Available renrinto ar e beins forwarded: 1, Typhus vaccine - attempts made to produce typhus vaccine from the peritoneal no: ibrancs of experimentally infected aninals. This project never reached a practical con- clusion. 2, Distribution of fluke infestation in fish - extensive survv>3rs of the extent of netayoninus, clonorchis unci other fluke infestations of fish lir.v. been ra.de, usiny a cl ice st ion technic to liberate the encysted stages for identification. 3, Sal:-.one 11a t'nn in Japan, 4. Jxperii xntal therapy of h. pranas chi infections in Guinea piys aimny a series of Hchinoir* derivatives prepared by the Hyashi Char: :accutical Company in 0!:ayr: :a, three have shown pror.ise in the treatment of piys expert*:entaily infected with Pichettsia prowaseki; oiuiYiu.;. Tc.cvltf of IlbTTciuc Hygiene Dc parti .cnt Dr. Ilasuo Drof, 01 Hygiene Prr.cticr.lly r.ll re s enroll sponsored by Dr, lies been concerned i. itli funder lent r,l i; ixmolooicel plicnoinenn, L Inr-de num- ber of re mints r.n be inn f orv/nrded under sc onrntv. cover. QIPPfPt R. 10.. IVl-RBITY Faculty of' LecTic ine S. Inada, Prof, of Ledicinc R, ICitayama, Prof, of Internal i.edicine 1, Professor Hitayama has directed a study in tlic clinical and irmuio logical aspects of influenza, They have be on unsucces ful in their attai.pt to develop a y.?od i;r.unization• 2 • The department has done r. yrea*c c.eal of work on the prob— leu of encephalitis Japonium. They have used a mouse brain antiycn but production was curtailed due to the war. 3. The professor (ICitayama) claims to have clarified the re lationship between the mid brain and the hypophesis ccribri. Ho has u.adc extensive studies of the blood Vessel and nerve fibre ; anatomy in the region of the tub arc -x :.er iun. He claims to have demonstrated a much closer neurovascular relrtionship between the pituitary eland and the neSenCeplielon thru other worhers. II. Dr. Hids-o- Yr.ci, Director or Gynecologic r.ncl Obstetric Clinic Doctor Yayi lias sponsored re search proyrans dealiny rath pro ole. _s oi i vji .alo s Guxili cy Gj. eaGi.eiio ■).*. c rcj.no—,a oil cervix and prevention of prenatal nd neonatal deaths. Rcprii of staff publications are boinn forwarded under sonarate cove hlule .studyiny the diaynosis of the causes of sterility, Doctor Yayi developed a modification of the Rubin test for tubal patency. His : ethod consists of instilllay sterile saline into one tube at a time,through, a dcsiyned instrunent which blocks off the tube not beiny tested, ‘flu volume and ra.te of flow throuyli each tube are charted and the yraplis obtained furnish comparative data on the patency of the tubes. Diayrans of the apparatus and sample charts are yiven in on.e of the above i .e n g i one d r e pr in c s * Doctor Yapi also maintains an excellent pathology nuseur for teaching purp oses, Ill, E, Hrjricu-.ir.to, Prof, of Paediatrics The professor’s staff was yiuatly curtailed during the war and he has been busy with clinical work. HeGently he has become interested in the study of Certain sulwhur proteins in mill: and serum. Reprints have been requested and i.ill be forwarded under separate cover*. XV. B. Er.tr., Trot, of Opthrli'ioloQy L, Pr.Jiurcv, fjso!t. Pi of, of Optlir.liiolo&y This doparti lent has boon conducting investigations into the problem of myopia. The37- are conduct ins clinical and animal ex- periments to show the effect of general diseases on the develop- ment of near si;phtodness. Reprints Trill bo forur.rc.cd under separate cover. Y. II. Ilr.yr.slii, -Prof, of Psychir.try The department of psychiatry hrs been conducting r clinicrl investiprtion of schizophrenia. Iirve shorn the CO9 tension of Cerebral blood to be low in aprrvrted denentir prrccoi: rnd rises on amelioration of the ort i ent’s condition. They liavo also shorn the temperature of the brain to be subnormal in schizophrenics. reprints i:ill be forv;r.rccd uncler scpr.rr.to cover# VI. II. Prof ♦ of UroloGy ond DcrnotoloGy The professor*s clinicrl reserrehes hrve been on the therrpy of syphilis, lie hrs rlso conducted many rninrl experiments on bismuth absorption rnd elimination rnd on the spinr.l fluid con- ccntrrtion of the nctrl, He hrs been attempting to find r method of improving the spinrl fluid rbsorption. lie hrs rlso done some experiments with rutb transplanted dor; hidneys nd on the effect of Vitamin. C deficiency on the susept- ibility of the rrbbit shin to infections. Imprints will bo fotmc.vC.oC. under scpr.rr.to cover. VII.* Si Tsudr, Prof:, ofoSpTGery Tlic processor has been v/orhinc chiefly on various seruii and urine tests tor cancer. Ho lias confirmed tho v/orlc of Fuchs and other Genian and Japanese workers in this field. He hrs rlso revised internet in the use of r colibrcillus rntivirus in the treatment of Peritonitis. The professor has rlwrys been interested in the problem of prncrertitis rnd he hrs continued his studies on this condition. Heprints will be forwrrded under separate cover. OKAYAMA. UNIYiA'SITY Faculty of Medicine Dr, Kaniohiro Oku sh liar, Prof, of Pharmacology Research for past five years was conducted in two Phases, First Phase constituted functional and morphological studies of vcrious organs, and the action of drugs, especially on the contra! nervous system. Second Phase constituted experimental and chemotherapeutic studies upon various infectious diseases, particularly Typhoid Dysentery, by the Aromatic Guanidin derivatives, especially P.oxybenzyl-guanidin, Reprints attached OlPPPd A. 111RV11P, iTY FFcuToy of medicine Dr, Hiroshi Tanabc, Prof, of Pathology Doctor Tanabe has been interested in the following probler and lias furnished available reprints to be forwarded under sen: rate cover: 1, Effects of the atonic bonb explosion in Eiroshirr. - Dr. Tanabe autopsied 19 patients in Eiroshina and 10 in Ohayaira, Reports are beinp sent to the Atonic Bonb Cor mission. 2, ?p.tholoGj;* of jr. virus cnccjJir.litif 3. Pathological clianpes in endocrine plands associated art hydropen sulfide or carbon disulfide poisoning. 4, . Irpsiolopy and pathology of endocrine plands, 5, Patholopy of interstitial pneumonia, 6, ..atholop'p of pul: .onary tuberculosis. Dr, Y, Eanazahi, hss’t, Prof. or Patholop3r Dr. Iloiiazahi has published an extensive series o:C histopath- ologic studies dealing with virus inclusion bodies and various intracellular structures d. canons t rat«d by special stains. Reprints arc bcinp forwarded under separate cover. OKAYAMA UNIVERSITY Faculty of Medicine * Dr. Kanae Hayashi, Prof, of Physiology Prof. Hayashi concentrated his studies on the effects of various conditions on blood vessel walls, for the protection and cure of arteriosclerosis. He claims that Vitamin C, upon Epinephrine-Arteriosclerosis pro- tects the blood vessel walls and cures arteriosclerosis. Reprints attached. OKAYAMA UNIVERSITY Faculty of Medicine Dr. Shimizu Taei, Prof, of Bio-Chemistry Dr. Kazuno Taro, Ass*t, Prof, of Bio-Chemistry Study on the physiology and chemistry of bile and, especially the origin and the formation of bile acid with 2A carbon atoms. The gall-bladder of different kinds of animals (fish, the water lizard, the snapping turtle, birds, otter and buffalo) were studied and separated various kinds of sterols (C26> C27, C28) or sterochalic Acids (C26> C27, C28) which have a constitu- tion of cholic acid or cholic acid itself. Furthermore, it was established that the other bile acids are formed from the cholic acid under oxyd-reduction in the animal body. Studies made on relation between the cancerous disease and the abnormal production of nucleic bases. (1) Nucleic base Uracil stimulates the growth of cancer tumor of the rat. (2) Nucleic base Uracil decreases Carbohydrate Metabolism of the rabbit. (3) The cancer tissue shows a remarkable decrease of Vitamin Bl. (A) The Uracil content is found abundantly in young rabbits and it is increased in cases of rats with growing implanted cancer tumor or in case of Vitamin A deficiency. Reprints attached. XXV. OSAKA IMPERIAL UNIVERSITY Department of Biochemistry Microbiological Institute Department of Pharmacology Physiology Laboratory Surgical Clinic Takeo Institute for Tuberculosis Internal Medicine OSd, 11 id.XX IIIXXXS10X Department of Biochemistry Prof. II. Ichihara Investigated enzymatic reactions, particularly tlie product- ion of indo 1 cf romcTr y ptopiiane by bacteria. Studied the enzynatic or.idation of ascorbic acid, dn outline of the v/orl; as well as re'print s ar c attaclied, OSAKA IMPERIAL UNIVERSITY. MEDICAL COLLEGE Dr* Tenji Tanlguchi, Director, Microbiological Institute Directly interested in virus and virus-like diseases from the standpoint of the elementary bodies and inclusion bodies* Princi- pally the relationship to pathogenicity, etiology and the life cycle. A listing of research for the past five years is appended* Re- prints on the completed phases of this work will be forwarded under separate cover* This institute has been engaged in the production of vaccines, antisera and antitoxins. None are different from those produced in other government laboratories. Buildings and laboratory facilities exhibit the unkeraptness observed elsewhere. The director. Dr. Taniguichi, from his works, is an inter- nationally recognized individual in the field of microbiology. A listing of his research publications to 1941 is included in the reprints• Has done work on exo evyfchrocytic malaria in chickens (gallin- accum). OSAKA IMPERIAL UNIVERSITY Department of Pharmacology Prof. M, Okagawa Assistants: I, Yamamoto E, Nichikawa J, Audo F. I to Professor Okagawa and his four assistants were interviewed* His most important investigations were studies on the pharmacology of adrenalin. He has studied the effect of vitamin C on adrenalin content of suprarenal gland and has published a paper on the formation of adrenalin from dioxyphenylalanine (the mother sub- stance ) • He studied over 100 drugs and essential oils as repellents for mosquitos. Those of value were capronic acid, caprilic acid, Shiso oil (from the shiso plant, grown in Hokkaido, containing 35% Pelllraldehyde) benzaldehyde, benzophenene, citronellaldehyde, and citronellyl acetate. The last three were the best. This work is not yet published. Attached is a list of more recent studies, as well as re- prints of their studies. OSAKA IMPERIAL UNIVERSITY Physiology Laboratory Prof. H. Kubo Investigation of oxidation-reduction potentials of various Biologic and Chemical systems* Attached is a list of papers published and 3 reprints* o&/g/. iiman mmmms Smgical Clinic Director: it. i’cie'bayaehi Investigated the detoxification of Sulfanilimides in the aniiial body, Evidence submitted that nicotine acid nary assist in the acetylation of Sulfanilinides. Three reprints attached Hidco Sei: Investig .ted the physiology of the spleen and has prepared a spleen preparation which caused an increase in red blood cor- puscles . wins preparation is in the experimental stage, but i s -jrc oarca as f ollow s: Crushed fresh cows spleens are washed with acetone and ether and stored in scaled containers. 10 such spleen treated withy' o h*g acetone, containing a trace of and then 1,0 IZg ether. flic dried material is extracted 5 times with 2p Ka 01 at 36'° C for 1 hour, centrifuged and filtered. An equal amount of' glucose is adder and the solution is refrigerated 21, hours, cry- stals separate 7 hich are claimed ' hen given in 1 gram doses causes the r. mid production of red blood corpuscles. second method for production of active material is to add 10 cc of water / 10 cc of Alcohol, containing 0.5p H 01 to 1 gram of dried spleen material. Crystal lil:e jiaterial forms on the surface, ...ttached is an outline oi this work. H. l\ nicvjc., Chief A. Ham, Assistant Investigated a blood clotting material prepared by treating cows blood v.ith Ip hydrogen peroxide, The foam is shinned off dried, dissolved in salt solution and prepared for injection, /japani.l.Urs■ forwrrdcd under separate cover. One or two ampoules of a 2°jo solution are said to stop bleeding from sserious rounds Investigated and prepared a hemicelluloso (Sulfuric Acid ester) union was found useful as a substitute for blood plasm. Sample under separate cover. Developed Histaninase and found it very useful in treating allergic conditions. Samnle under separate cover. OSAKA IMPERIAL UNIVERSITY, MEDICAL COLLEGE Takeo Institute for Microbial Disease (Tuberculosis) Dr* Avao Imanrura, Director, 3rd Clinic for Internal Medicine, Osaka University; Director, Takeo Institute for Tuberculosis, Osaka University* Dr* Imamura is primarily working in tuberculosis, as director of the institute* He has been instrumental in the initiation of the B. C*G* vaccination program in Japan. From his statistics, over a fifteen year period, a definite reduction in the incidence of tuberculosis among the vaccinated is shown* By means of a mobile laboratory, over 700 thousand individual examinations of civilians from infancy to old age have been made, in determining further the prevalence of tuberculosis* The work in progress at present is principally directed to- ward developing new attentuated M. tuberculosis hominis strains by cultivation on a glycerine-gall-potato medium. Four strains showing a high degree of attenuation by animal experimentation, have been developed* These have not been used in humans. A so-called spectroscopic diagnostic method for cancer, using an extract of cancerous tissue and serum, has been developed. Reprints of research will be forwarded under separate cover. A digest of the cancer diagnostic method is appended* OSAKA D JPSRIAL UNIVERSITY Dr, Arao Imamuro, Frof* Internal Medicine A specific Acid Turbidity Reaction Test for cancer diagnosis has been developed. The method, in Japanese hands, shows promise. The ■procedure is attached. Also, attached are reprints of III Clinic for Internal Medicine, xxvi. Toizro jikeinai medical wxvmsun Dept, of Forensic Medicine XXVII. AGRICULTURAL EXPERIMENTAL STATION Director of Research XXVIII. JAPANESE A2K3T DEPOT FOH MEDICAL MATERIAL R o s garch D e;ot 6 XXIX. RESEARCH INSTITUTE OF TUBERCULOSIS Dr, H« Oka, Director XXX. WARTIME RESEARCH COMMISSION Bo ard of Toonnioal Science SDCX. EDUCATION jtlEQ.UIREMENTS FOR t.IEDXCAL PEG-RES - Igakusi TOKYO IMPERIAL MEDICAL UNIVERSITY Department of Forensic Medicine Prof, Mitsuteru Ishikawa Professor Ishikawa investigated Anaphylaxis in animals since 1934, with the object of its application to the prevention and treatment of allergic diseases, though no clinical applica- tion has been made to date. Little attention to date has been made by the clinicians to allergic diseases and their treatment. Recent work has been on sensitization of animals to drugs and chemicals, somewhat similar to that of Landsteinerfs work. He has attempted to differentiate between chemicals causing skin sensitivity alone, or those causing skin and intestinal muscle sensitivity in guinea pigs. For example, aspirin and Barbital, quinine, chromium compounds, cresols caused only skin reactions while Iodine, such as lugols solution produced both skin and muscle sensitivity. He was unable to sensitize animals to thyroxin. Attached is an outline of the research activities of Jikeikai Medical University, also the collected research papers of Prof, Ishikawa on Anaphylaxis. AGRICULTURAL EXPERIMENTAL INSTITUTE Dr. Jeijiro Yabuta, Chemical Engineer, Director of Research Investigations of interest were the studies made on Vitamin Bi and C: Vitamin Bi was obtained from peanut skins and Dr* Yabuta states that is contains about 7000 units of Vitamin BI in 100 Gras, as compared to rice embrio which contains 4-500 units of Vitamin B]_ in 100 Gms, Vitamin C was found present in Persimum Leaves and in Walnut Leaves from 1% to 2$ on the basis of dried leaves. Reprints attached. HOME ISLANDS MEDICAL SUPPLY DEPOT - YOGA (Tokyo) Research Dept, Lt, Col, T, Yamanouchi, Director of Research Research Plan since 1940 constituted studies on Cardiotanics, disinfectants, anesthetics, sulphanamides. Vitamins, anti-malaria remedies, and investigations for substitutes of chemicals and pro- vision against lowering of specifications. Investigations of interest are as follows; 1, Caffeine sodium acetate and sodium naphthaline-sulphnate were produced experimentally to economize the usage of toluene, the raw material for caffeine sodium benzoate. 2, Synthesis of dimethyl ester of phthalic Acid. 3, Simplified method of obtaining local anesthetic by the combination of ]>-aminobenzoin ethyl ester and p-phenol-sulphonic acid. 4, Synthesis of sulphabenzotriazole for a new chemothera- peutic medicine. 5, Synthesis of thiazole nucleus for vitamin B1 without the use of phosphorus compounds. 6, Utilization of butyl ester of gallic acid to prevent oxidation of vitamin A, 7, Promotion of the therapeutic effect by the modification of quinine (seven new effective compounds have been obtained). 8, Simplified method of manufacturing disinfectant from pine root oil* 9, Simplified method of obtaining Vitamin Bi bran rice and wheat germs or rice bran. 10, Examination of the use of Nerium Odorum, a cardiotonic. 11, Manufacture of vitamin Bl from the waste liquor obtained during starch production. 12, Manufacturing method for intestinal medicines by anti- septic butyro-bacteria. Reprints and sample of Bacillus Butricus enclosed. RESEARCH INSTITUTE OF TUBERCULOSIS 1005 Shimakiyoto, Kiyose, Kitatama County, Tokyo Director: Dr, Harumita Oka. This modern appearing laboratory is one of the important centers for tuberculosis research. Dr. Oka has headed a 24 roan committee which has been responsible for giving BCG vaccine to more than nine million people, exclusive of the Army and Navy, This committee has studied a group of 100,000 Japanese receiving the vaccine and another equal size group in another area which served as a control. Using repeated tuberculin tests as the basis for X-ray exam- ination during a 5 year period of observation completed in 1943, the following general conclusions were drawn: The 100,000 people receiving BCG in certain areas showed only 50% of the XOray evidence of infection seen in the control areas, l/U the clinical evidence of infection and 1/7 the death rate. In the group receiving BCG, the per- centage of extra pulmonary lesions was reduced and the usual peak death rate between 20-25 years of age was flattened* The vaccine is made from the original BCG strain brought from France, This culture is maintained on potato bile medium. When vaccine is to be produced, the organisms are cultured two weeks in Sautonfs fluid, dried somewhat on sterile filter paper, weighed, suspended by shaking in Saline and gelatin and dispensed for immediate use. The vaccine is outdated after seven days. It is administered intracutaneously in a dose of 0.1 cc which contains 0,04 - 0,06 rag, of tubercle bacilli. It is only given to tuberculin negative individuals. It is not given subcutaneously because of the danger of abscess formation. The age groups most extensively studied have been 15-25, Dr. Oka has furnished english summaries of research publica- tions, copies of the publications and reports pertaining to BCG and a stock culture of the BCG culture used to produce the vaccine, (Additional reports on work with BCG vaccine, have been accumulated from other sources and reported under other insti- tutions). Stock culture carried to Surgeon General*s Office by Lt. Col, Henry Cotton, on IS November 45. Reprints are attached. English summaries will be forwarded under separate cover. WARTIME RESEARCH COMMISSION '(Bo'arcT of Technical Science) This appears to be an overall scientific development and research organization which is responsible to the Cabinet, Its governing section is The Chamber for Technical Sciences. The Chamber for Technical Sciences,':, is composed from the Vice Ministers of Cabinet positions and from Technical services such as Aviation, Shipbuilding, and Ordnanace Administrative Headquarters. In fell there are 25 members. This chamber decides upon what projects shall be undertaken, allots funds, decides upon priority, an