Dec. 13, 1992 Dear Dr. Sokoloff How are you? I think I spent must exciting time in September since I returned to Japan. I really enjoyed your clear lecture so much. I would very much appreciated receiving a quick answer to my questions. Since I returned to Japan, I started to set up experimental room to measure Vmax and KT. I bought a Beckman glucose analyzer (this is very expensive in Japan!) and microfuge. I set up glucose clamp technique again. In my hospital it is prohibited to use radioisotope to experimental animals. I got permission to sue a RI experimental room in pharmaceutical compnay. I will start RI experiment coming January! I want to show results and want your comment when you come to Japan next year. I am very much exciting now. By the way, I want to ask question again. According to your letter, PSMG x Cp - MG = VT - MG - PSMG x KT - MG x (1 + Cp - glu/KT - glc) You said "All units must be in molecular concentrations of methyl-glucose and glucose." However, I will be use radioactive methylglucose in defferent plasma glucose concentration. So that I have only data of tracer concentration of methyglucose. How can I manage Cp - MG in this equation? I am confusing. Would you answer to my question? P.S. Recently I became assistant professor in my department because I learned a lot in your laboratory. Sincerely yours Kentaro Mori