date Tit Cloning record Loot

ist Cloning » end ik

designavion;.
n of cells
“Sure started /
tt : 4g
es of DaASSARESs WH!
Media used 3 media - |
HS : &

FCS %
6) passed in agar f= 033%/05%

  

e/ eeee
eeoee 7) . Rémarks; cell moronolesy

cf

ist
2ne.
3rd
SoSH STEHT OH SHEE OEATHOSC SETS EOHSCTHEHA DAHER ETH EME SEH S THEO E OHH ESC SHEATHS AS
8) Preparation of cell suspension Gace Le
9) last media change 5 / eocetile
) last subeulture /
)

HO

aters pooled, ‘eombined with stickexs

ee Te er

washed with. % of trypsin in....Dl or D2 LOCH cseeccecvscccces
eeeettl. madc on

Hes

x

jv
%&
—

13) incubated with ...ml. of .....trypsin
2

14) spin [cao LPRecccseeiOle Jonin. at 4- C CENULLLULS ave wvevcccees
15) resuspended in.....ml of media DMEM. Lott 1O3O..

( HS 200 000% Lotwec Tb bbSieece
FCS. coe elo eS LO Gite « Uri « «
16) cell count x 16 “4% Gil factor » fom.
Viable/deadercecvccccss/eecscucusvevees

17) diluted COcccccceccecec Sse /al. Wath media

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18) plate used 4002 ) ececccsevd eee an so.
3002 ot

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5 see ee Ue a ee wb OL VRP GLbeowes
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oy
Plat 7.

3
dae ine, - Sele

wee e ee: Cob eee weer eee GSC e cece ee PLE UW site ces ee Ce eee eee ee eees
of plating of cylinder | v DL oad eis, eplind
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