APPLICATION FOR APPROVAL OF RESEARCH PROJECT INVOLVING RECOMBINANT DNA

Name. and title of principal investigator: - Joshua Lederberg, Chairman & Professor of Genetics

 

Department: - Genetics

JP ave rien nate nam me

Title of project: - Genetics of Bacteria
. Research support (agency and grant number): - NIH 5 RO1 CA16896-18

Se of project: 1) To understand factors regulating expression of bacterial DNA
; in another host
2) To clarify the mechanism of genetic transformation

 

Source of DNA to be cloned:

1. Species Bacillus subtilis ; E. coli K-2 (non pathogenic bacteria only)
B. subtilis_PHAGE phi-3-t (a small genome) for most expts.
2. Organ or tissue, if relevant

 

3. Extrachromosomal or organellar genome, if relevant

 

_ Degree of purification of donor DNA (eg. shotgun cloning of total DNA, prior purifi-
cation of organelle, purification of CCC DNA by cesium chloride-ethidium

bromide centrifugation, cDNA emade from RNA, etc.):

Variety of methods. Uspaldy eStaiepectEopnerssis banding which affords substantial

Assay for purity (if relevant to the containment level proposed) :

Vector(s): mini-colEl lambda pscl01 phi-3-t s

.. Recipient species (and strain if relevant) (eg. E. coli C600, X1776, B. subtilis, etc.):

E. coli K-12 B. subtilis

 

Level of containment proposed for project: P2-EK1 for routine experiments on
pre-isolated clones
P3-EK1 or P2-EK2 (new vectors) for any new 'shotgun' efforts, pending resolution of
the applicability of this requirment for the present cases

Physical containment level currently available for use:

 

P2.. P3-modfication of a laboratory is in progress. (We are also enquiring
about borrowing use of other
facilities.) ,

” Section of NIH Guidelines relevant to proposed containment level:

 

page 35 "Prokaryotes that do not exchange genetic information with E. coli"

(We.are still investigating this criterion; and are uncertain whether. it was really
intended to apply to B. subtilis -- E. coli K-12)
Research Project Application Cont'd Page 2

Project personnel (names, titles, and responsibilities)

Joshua Lederberg, Chairman & Professor
Stanislav Ehrlich, Research Associate
Hela Pettegrew, Senior Research Assistant
Peter Evans, Lab Technician

Jerry Feitelson, Graduate Student

Grace Chi, Postdoctoral Fellow

. Iwona Stroynowski, Graduate Student
Matia Contreas, YOP Student

Hung Pham, Lab Technician

Have all laboratory personnel working ‘on project received instruction in _appropriate
containment procedures? yf

Are all professional personnel familiar with the NIH. Guidelines?

Check one: ee

{ 4 1. The recombinant DNA molecules described above have already been constructed.

 

 

 

{ ) 2. Construction of the recombinant DNA molecules described above is proposed.

Comments :

I agree to abide by the provisions of the NIH Guidelines for recombinant DNA .
mlecules and that the recombinant DNA molecules being used will not be trans-
ferred to other investigators or institutions unless their facilities and tech-
niques have been assured to be adequate, and a new MUA is executed by them and

submitted to the NIH prior to the initiation of experiments.
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NOV 10 1976 Lhe ADD

Date ff ” Principal Investigatér