THE COLORADO FOUNDATION FOR RESEARCH IN TUBERCULOSIS

 

 

 

GERALD B. WEBB MEMORIAL BUILDING 4200 East Ninth Avenue
Denver 20, Colorado

 

September 6, 1956

Dr. Joshua Lederberg
Department of Genetics
University of Wisconsin
Madison 6, Wisconsin

Dear Joshua and Esther:

I am glad the proofs of #2 are fixed up and the reprints ordered.
The market hasn't been too good on #1 (I haven't filled a request since
coming here), You may sent out reprints on your regular list and I'll
fill the reovest cards that come in, Remember to subtract the list that
I left from yours so as to avoid duplication,

Sorry I missed in the proofing. 7 haven't located your comments,
but will when I recover my copy of the proofs from Lerman,

I haven't seen the Goodgal paper as yet and as it is easier to
keep up with the literature here than in Madison, perhaps it hasn't come
out yet. There is a paper by Kaiser (C.R. Acad, Sci. 22, 3129-3132)
that may interest you. It has to do with lysogenization withelytic(@
mutant(s) of lambda, and may be useful in studying segregation.
Apparently, exposure to both a lytic anda non-lytic mutant results
in some clones containing both markers. What this really means I
don't know, but it might prove of value.

With regard to the penicillin effects you are studying and the
applications you once suggested to tuberculosis, penicillin (100 units/ml. )
4s quite often included in isolation media, but I haven't found any
quantitative statements to indicate that there was better or more
growth. Incidentally Zamenhof in his review mentions INA transformation
in tuberculosis. ST -x S84 5S"

I received a note the other day from Kurahashi and a copy of
Kalcka®!'s talk at Baltimore. Kalcha&'s words are a bit confusing
but the chemical data appear straight forward, if not ideal, I have
answered and said I was looking for mutants for the other biochemical
steps, and presumptive cases I would send him. Thus far I have decided
that the only thing to do is to look for those not giving P.E, with
either Galo or Galj,-. With regard to your comment on the Waldenase
mutants, we don't know that Gal- mutants of this type have galactose
in their cell walls (do the Gal+?) and T have always had reservations
about hydrolyses products representing "unhydrolyzed structure" or
necessarily having anything to do with steps in syntheses.

 
Dr. Joshua Lederberg @eeererreonseeaee eee senses eeeeoeeeoeeveostoeoeneses Page 2

But I suppose it is better to use some than no information in setting
up a plan of attack. I'll keep an eye out for galactose dependence.
Replica plating would be nice but silica gel glucose agar doesn't
sound like anything I want to make up.

By the way, where did you buy those valves for the aeration system?
I haven't been able to find them anywhere. I need a few for my lab, but
hope to do as muchas I can in rotatimas., The only place to locate
rotators would be downstairs in the 37C walk-in and not very handy for
any experiments requiring sampling at short intervals,

One of the other members of the lab has a tape recorder and has
offered to let me use it. I may send you some words on tape shortly,
after I have obtained some small reels.

With regard to the address, Webb Building, University of Colorado
“edical Center, Denver 20, is about as short as it can be gotten,

Sincerely,

M. L. Morse, Ph, D. (11)

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