Aug 25
Dear Josh and Esther
So far is a good fellow; we will miss him we he leaves.  He has been witnessing a lot of difficulties with respect to our analysis of nucleotides and Gal-1-P.  Determination of enzyme "Titers" are much easier, except if the Titers are small and high accuracy is required.  Determination of bacterial deproteinized filtrates is complicated when it comes to using enzymatic method.  I have decided to leave out of the present two papers analysis of complex polysaccharides, ATP, UDPG and Gal-1-P.  In a few weeks we shall have most of these data on solid ground and will submit a detailled [sic] manuscript to J.B.C.  Meantime the hereditary enzyme defects, galactosidase and the microbiological aspects of hereditary gal. sensitivity will be communicated to Proceed. National Academy of Sciences within a week.  I am fed up with having these scripts around and since it is mainly due to lack of reproducibility of ATP and[?] Gal-1-P analyses the obvious way out is to collect all these analyses in one or two new manuscripts.
In Gal 3 there are many funny observations.  B. galactosides are not able to induce the "[ . . . ] enzymes."  Free gal neither -- but both are able to induce B-galactosidase.  The "lytic" strain CTM on the other hand
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reacts the other way.  Galactose induces transferase or gal kinase (epimerase is "out") but scarcely any B-galactosidase.  Propyl-B-galactoside* induces B-galactosidase but not transferase.
Paper I and II (for Proceedings) are being retyped.
I'd better finish for this time.
Sincly
Herman
*Excuse handwriting but it is in B.O train and they are trying to hook a locomotive on!