DEPARTMENT OF HEALTH, EDUCATION, AND WELFARE

 

PUBLIC HEALTH SERVICE 6 BETHESDA 14, MD.

 

NATIONAL INSTITUTES OF HEALTH

February 2, 1958

Dr. Joshua Lederberg

The University of Wisconsin
College of Agriculture
Department of Genetics
Madison 6, Wisconsin

Dear Josh:

You are right about your little footnote. It didn't occur
to me that there could be a problem. I have used up my money
for meetings and I am barred from extramural grants. I happened
to have a chat with Bill Consolazio of the National Sétence
Foundation about another matter and brought up this problem. He
said that there may presumably be a chance of National Science
Foundation reimbursing the University of Wisconsin for paying the
expenses of NIH scientists participating in the meeting. Perhaps
I am the only one or, at least, the only one who has problems as
usual, You had better check with Bill Consolazio rather than take
my word for it, but perhaps you have various devices anyhow as to
how to solve this problem.

I am in the midst of work on your mutal of 'gal 3' (3264).
I have been growing it on mineral medium containing glycerol or
glycerol plus galactose as carbon source. I am trying to improve
methods so that they are more accurate in the assessment of leaky
mutants. Interestingly enough it seems, in this case, that the
UDPGal-4-epimerase is present in considerably larger amounts in
the population grown on galactose glycerol as carbon sources than
in glycerol alone. This is, so far, the only point which is at
variance with Kiyoshi's findings in nutrient medium. We have also
some promising studies on yeast 4-epimerase. I shall write you
fairly soon about these problems.

Kindest regards to you and Esther from both of us.

Sincerely yours,
ve ‘ ' ny Se He.
ty’ pe

Herman M. Kalekar

HMK: ih
National Cancer Institute National Heart Institute National Institute of Allergy and Infectious Diseases National Institute of Arthritis and Metabolic Diseases
National Institute of Dental Research National Institute of Mental Health National Institute of Nevrological Diseases and Blindness The Clinical Center

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