UNITED STATES DEPARTMENT O AGRICULTURE B SERVICE OFFICE OF EXPERIMENT STATIONS MAYAGUEZ, PUERTO RICO FEDERAL EXPERIMENT STATION April 7, 1964 IN PUERTO RICO Professor Joshue Lederberg Genetics Department University of Wisconsin Madison, Wisconsin Dear Professor Lederberg: Dr. Warmke has asked me to assist you in your search for a small supply of the glucoside “indican". The two species which are reported to contain appreciable amounts of indican are Indigofera tinctoria and I, suffruticosa. Both of these species grow here in Puerto Rico. . Our experimental plots have a stend of I, suffruticosa, but unfortunately this legume is seeding at the present time. It has little vegetative growth, and would not yield sufficient material at the present time, I. tinctoria grows wild in Puerto Rico but none could be found near Mayaguez. We hope to collect a larze sample from the southern part of the island within the next few weeks, ‘Ve will dry and mail you the plant material, At that same time we will also try isolat- ing the glucoside from another sample of I, tinctoria and if succes- sful will mail you the indican which we cbtain. During this long period of delay you might be able to get a small semple of indican from the Hawaiian Experiment Station. The Poultry Husbandry Department there fed this glucoside to chicks during their investigation of the toxicity of I. endecaphylla, te Yours truly, Dhar uch’ “ Mg Mra Murrell P,. Morris Chemist