JUL 16 1969

NATIONAL INSTITUTE OF GENETICS

YATA 1111. MISIMA, SIZUOKA-KEN,
JAPAN

July 10, 1962

Dr. J. lederberg
Department of Genetics
School of Medicine
Stanford University
Palo Alto, Calif.

Ue. Se A.

Dear Josh,

Thank you very much many helpful informations in a series of
your letters. During the early stage of flagellar regeneration from
deflagellated cells of a phase-2 stable (H,°Vh,H,°™) strain, phase-]
{a-type) antigen could not be demonstrated by ordinary agglutination
test. Insteads, I obtained some interesting (though still preliminary )
results which might be useful to look inté the mechanism of Ho-xupreagkex
epistasis and flagellar formation.
(1). When phase-2(1.2-type) flagellin prepared from Ah “Hy 2Ho1-2 is
added to the regeneration medium for monophasic-1 (Hy ott 2afio™) cells,
the cells in early regeneration stage agglutinated by anti-1.2 serum as
well as anti-i serum.
(2). Antiserum prepared against stable phase~2 (Hy“H,°2X vho~) cells
contains both anti-a and anti-enx antibobies; while that against stable

phase~] (Hy 9H . AL cells contains enx-agglutinin but not a-agglutinin.
i Hy °

(3). Anti-Ahy"H cell serums so far have been tested contains
anti-phase-2 antibody but lacks anti-i antibody.

(4). Antiserum prepared against TM2 cells deflagellated by phenol contains
i- and 1.2-agglutinins.

Now I am inclined to think that,
(1). The site of flagellin synthesis and that of the flageller formation
might be different. The deflagellation effect of phenol is on the latter.
(2). In phase-2 cells of diphasic straihs, both phase-1 and phase-2
flagellins are produced. At the flagellar forming site, phase~2 flagellin
and phase-1 flagellin copete and the former represses the incorporation
of the latter to flagella.
(3). Ah; (and 4h, also) regulates: the activity of H, (and Hp respectively).
In Ah)~ cells, H, is inactive and phase-1 flagellin is not synthesized.

I expect I will be able to obtain more results for or against these
hypotheses befdre my departure for U. S. A., and to discuss with you on
them based on the more materialised data.

This sumuer,Miss Mitani, a graduate student of Tokyo Metropolitan
University, and Dr. Dhillon, an instructor of Hong Kong University and
a friend of Dr. Ganassan, are visiting ny laboratory. They wiht work
with me here till the middle of the comming August. Mitani started the
studies on the effect of antibiotics on the flagellation; Dr. Dhillon
is interested in looking for the transduction between E. coli and
Salmonella, and Hft of motility genes.
NATIONAL INSTITUTE OF GENETICS

YATA 1111. MISIMA, SIZUOKA-KEN,
JAPAN

The outline of my itinerary in UJ. S. A. is as follows:

August 18. Leave Tokyo for Montreal directly
18-29. in Montreal and Stowe
30-Sept. 9..Trip thru U. S. A. via. New York, Lafayett,
Madison etc.
Sept. 10. Arrive Palo Alto
20. Leave SanFrancisco for Tokyo.

The probability that I will accomany my wife is now above 90%
I should be very hapCpy if you would reserve a dormitry room for
us ten days from Sept 10.

Best wishes to Esther(Hoshi-ko San),

Sincerely yours,

ye
J bx

Tetsuo Iino