June 1, 1958
Dear Josh and Esther,
I enclose two pictures of Dr. Ryan.  They were taken at his apartment room.
I found the culture of SW577 (described as 90% curly) changes phase between curly and normal.  That is, the culture is curly in phase 1 (i) and normal in phase 2 (1.2).  The strain seems to be a mutant of H1-Ah1 region.  The detailed genetic analysis is in progress.  As Dr. Kerridge in this lab has found, the replacement of p-phenylalanin by p-fluro phenylalanin at the regeneration of flagella in phenylalanin requiring mutants of T142 + SW1061 causes the production of curly flagella.  If the similar mechanism is involved in the curl in SW577, the strain might have a modified ability to incorporate a specific amino acid into H1-protein.
Best regards to the people in the lab.
Sincerely yours
Tetsuo Iino