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Deaf HeadPberg,ivitiense ser-AVG yldedesy foe, ero 70 monem

Your letter of Feb.15 has been received; it
crossed over with mine. As conésrns a joint paper,I shall
send you a draft giving details of all my experiments related
to the problem as soon as I come back from Britain - the llth
or l2th of March. Unfortunately I am leaving tonight and cannot
do it before. I am sorry to leave the burden of wr’ ting the
paper entirely to you, but the excuse:of the knowledge of the
language,is, I hope, a sufficient one! I shall see Hayes in
London; he has written to me that the experiment he planned
in his letter,which you know,has in fact confirmed infection
of F+ . I should suggest quoting his independent result,and
should ask him more details about it,H#X

I have not yer repeated the experiment with
purified DNA-ase : none available here. I hope to get some in
Britain. I also hope you have a chance of confirming this point.
; shalt of course be repeating this experiment as soon as I come
ack.

I hope Hfr has reached you in good health. I have
peen unable to obtain transformation of F- cells with Hfr, but
have tried only once. Another interesting fact is that some of
the F- strains om which F+ has been transduced seem to give a
high frequency of recombination (twe out of six tested),while
the others give a low frequency! Perhaps we are dealing with a
mixture of transducing principles,or recombinations of them.

It is not yet clear to me what Hfr can mean,if,it does not - as
it does not seem to-follow your hyxrothesis of /powerful F+ donor.
However I have a sort of fe@ling that some explanation can be
found.

You will not,I hope,be too/BaSPisea of a sentence
which I wrote to Hayes in the letter of wh ch I sent you a copy.
As a matter of fact,xme I still believe that recombination and
infetbion are entirely separate things ,as is fairly obvious
from all experiments sofar,but I have been astonished by the pos-
sibility of obtaining genetic infection with suck a high yield
in the absence of trans-‘orming principles in t filtrates - or

any Bkker sort of lysate,VvI nave had some? post iYe results, but

rather difficult to reproduce. after all, besides the fact that
recombination involves a correlated change in a number of markers
which is of course &he fundamental difference, and that the

exchange is reciprocal, the other differences between recombina-
arte.
=
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Ra Ae Ge
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OSAGM 1G Gta Ata Olasieds asia Ts
GO "UWIWFAAG AIN ~ GUAGIM

‘tion ana tntection remaing the "frequency with which eather pheno-
menon occurs,and pevhably DNA-ase sensitivity. Not... much!

a _ Yours sincerely,

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