DEPARTMENT OF BACTERIOLOGY S20 HENRY STREET DETROIT, MIGHIGAN January 17, 1947 Mr. Joshua Lederberg Osborn Botanical Laboratory Yale University New Haven, Connecticut Dear Mr. Lederberg: In reply to your letter of January 13, please be advised that we have no information on the vitamin content and other growth factors present in our Bacto-Agar or our Special Agar (Noble). Special Agar (Noble) is prepared by washing Agar in distilled water, and we do not know if this would remove all vitamins or growth factors present in the Agar. I do not believe that there is any other source of vitamin-free Agar. Sometime last summer, we had occasion to wash some Bacto-Agar with methyl alcohol. It is entirely possible that the washing of the Agar with methyl alcohol would remove some vitamins. Yith this thought in mind, we are taking the liberty to forward you a 1 oz. sample of this experimental washed Agar, R74622A, with our compliments. Should you have an opportunity to use this Washed Agar, Exptl. in your work, we would appreciate knowing of your results. Yours very truly, H.W. Schoenlein ssistant Director HWS :2VA