June 75 1955

Dr. Einar Leifgon

Stritch School of Yedicine
Loyola University

Chigago 12, Dllinois

Dear Dr. Leifson:
Thank you for the reprints and your letter.

The experiment I had in mind was aimply to isolate cells to single
drops so that I could watch their motility as they divided. If the flagella
lag behind cell division, one cell should remain motile, the other (demghter?)
would become motile only after a time. This experience should parallel
cytological determinations of the polarity of flagella.

If you have any interest or intention in this direction, I will happily
defer to &t, or to collabora te in any other way. If you are not interested
in this approach yourself, I would be grateful to you for appropriate cul-
tures, namely those which are predéminantly monopolar and bipolar respectively
during active growth. Might I leave to your own judgment the choice of the
most suitable strains for this type of experiment? It would not be difficult
to screen a fair number (not too many!) of strains if the choice seemed
equivocal. It would be best, I think, to compare "monopolar" and "bipolar"
strains of taxonomically related organisms.

ougs singer ‘ely,
cs
L

ot

/? Professor of Gepst

if