LE av
Resaarech Ldees-

1. Implant en egge or emryos of frog in sbdomen or onto omentum.
Determine influence of 'o0lda' environcent on growth of embryonic
material, and vice verse. 1. look for rates of development of the

embryos, and possitle development of teratomata.

2. Tissue culture of egf cell: in Arbacie, using Ca free seawater

if necessary to induce separation of blastomeres, attempt to induce
indefinite proliferation of the egg eell by using varioue medie. This
should be wothout differecntédtion or orgenizetion.

3. Cukture of grasshopper testes--- whatvinduces the sperme togoniun

to stert its cycle of spermetocyte end differéntie tion into spermetid
Can sperm-formaéion be induced in vitra? Egg?? Serious prectical

and pio-philosophicel consquences:: the perpetuetion of the germ without
the OR «

The influnece of respiretory inhibétors on the mitotic figurel Look
for a meterial in which the mitotic funetion is pronounced, end which
is amenable to reppiretory and chemical enelysis. Even the old one
of cyanide on onion root tip would be intersting. W’rk out a cese here
even now???Why bother to introduce the compliece tion of colchicine.

Repeat the electridynamic mpperiments of MeSlendon ete, and determine
whether the root 8 really remain elive. One pesumptive test might be
the assumption of the c-figure on subsequent treatment with eolchi-
eines Reversibility is the impirtant factor. If there is » fairly
simple electrical polarization in the root tip it should te demonstrak
as follows
 

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