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Supplement to the "FURTHER DISCUSSIONS ON THE EFFECTS OF PHASE DIFFERENCE
ON THE H-ANTIGEN TRANSDUCTION IN SALMONELLA DIPHASIC STRAINS?"
--- THE EFFECT OF PHASE-VARIATIONS IN THE TRANSFORMED CLONES ON THE YIELD
OF H-TRANSDUCED TYPES,
Tetsuo Tino
( July 28, 1955 )
As has been reported already, the frequency of each type produced by the

transduction of H,-or H,-factor is as follows,

fh, --—--— ry (1")
Hy By -------- t2d, (2')
fH, -------- ty, (34)
nh——

The abternative:.< types and their frequencies produced by the phase variation of

these types are

H)H, -------- atyr) (1")

Hh, -------- dtodo (2")

*

Byhy -——--— Bty%y on

HH -------- atody (4")
phase-1

a = Rate of A(/transformed clones,which contain phase-2 cells produced by
the phase variation during the course of the screening, to the total
transformed phase-1 clones.

bd = Rate of transformed phase-2 clones, which contain phase-1 cells produced
by the phase variation during the course of screening, to the total
transformed phase-2 clones.

When antiserum for the H-antigens of the recipient cells are used as selective
agents, type (1") and (2") are not detectable as well as type (3%) and (4%).
Thus, the expected ratio of phase-1l transduced type to phase-2 transduced type is

z, : i = ty (r) + bry) + to(do + adj) (6).
The transduced types when donor, recipient or both contain only single phase are
shown in Table 6. Table 6 indicates that, if phase-variation occur during the
screening of the transduced types,
(1). Ho-transduced type will appear on the "l-#x'1",
(2). Hy-transduced type will appear on the "2 -x 2",
and (3). both transduced type will appear on the "1 -x 2",
in addition to the types which appear when phase variations don't occur in the
transformed clones; but
(4). the ratio of i, to Hp will not change by the phase~variation of trans-
formed clone during the screening of transduced types, and express
directly the ratio of the transduction efficiencies of Hy and Ho.
In other words, when phase-2 is used as donor and phase-1 as recipient, we can
eliminate the influence of the phase-variations, which occur during the screening,
on the yield of transduced type, and can discuss the transduction efficiencies

directly from the number of each transduced type.
Table 6.
The results of H-transduction between diphasic strains, expected from the
proposed hypothesis when selected by the antiserum for the antigen of the
recipients. ---(2) ‘The expected results when phase-variations occur

during the sereening of the transduced types,

 

Phase of Phase of _Ratio of Hj-transduced type to H.-transduced type
donor recipient when phase variations (wher phase variations don't

 

 

occur in the trans occur in the transformed
formed clones) clones
Hy > HL H, :
1&2 L&2 ty(rytbro)  t, (dptady) t)T, tnd,
1 1&2 t,(r,+or2) at2 tT) 0
2 1&2 ty (x, +bro) to $F) to
1&2 1 ty tp (dotady ) ty tod,
1&2 2 dt, t2(d2tad, ) 0 tod,
1 1 t) ato ty 0
1 2 bt, ats 0 6
2 ? *y be ty tg