ADDITIOwAL DATA OW TRANSDUCTIONS OF "FLA" AND "H" FACTORS FROM SINGLE

PHASE CULTURES OF SAL. ABOMY (Sw803 Fla* brenx) TO SAL. TYPHIMURIUM

 

$W1157 Fla~ 4:1,2).
Report by Tetsuo Iino
March 28, 1956

In the foregoing report ou transduction SW803 (Fla* b:enx) ---x
SW1157 (Fla™ i:1,2), mumbers of linked transduction type were small, and
it has been desired to accumlate more data to get statistical completeness.
In the present papers, additional result obtained by the repeated experiment
were reported, and wewe summarized with the previous result.

Materials and methods are the same as the previous experiment.

Table 1 shows the additional result obtained, and Table 2 shows the
summary of the previous and the present results. Both results coincide well
each other. The frequency of linked transduction of H, to Play) 57 is fairly

homogeneous.

SUPPLEMENT. Stability of SW1157 and H-phase of reverted cultures.
Nomber of swarms per brush recovered in above transduction experiment
4s shown in Table 3. In seriesesuviehtbhwbyeaté and recipient culture were
mixed, 4 to 7 swarms grew per brush in average. In the control series,
swarm did not appear in 20 hours (when the isolation of transduction swarms
was made), but when they were incubated farther (at 376) the growth of
swarms are observed in 48 houes, as numbered in parenthesis in Table 3.
Slide agglutination test of penassay broth cultures of these swarms has
shown that they are in a same phase as supposed from vreliminary transduction
test. In parallel with these experiment, the same cultures used in transduction
experiment were inoculated to MGA-stabs. 15 subcultures were prepared on

each phase (0.05 ml per stab), and growth of swarm was watched incubating
at 37C. Only 2 stabs in supposed Fla™-i cultures and 1 stab in supposed Pla™-1,2
cultures grew swarms. Their antigen types were tested by slide agglutination
after transferring to penassay broth. 2 swarms developed from Fla -i have shown
i-antigen reaction and 1 swarm developed from Fla -1,2 has shown 1,2~antigen
reaction.

These results indicate that Fla‘i157 revert to Fla’ in sone frequencies
but the frequency of reversion is very low as compaired with that of Fla-
transduction, aud practically it has no influence on the result of Fla-trans-
duction experiment. The results also confirm the Fla”-cultures used in the
experiment have alternative H-phase specificity, althhugh it is not expressed,

just same as supposed from preliminary transduction test.
Table 1.
Traneductions of Fla and H factors from single phase cultures
of Sal. abony (SW803 Fla* b:eux) to Sal. typhimurium (§W1157

Fla 1i:1,2).--Fla Flay4>57 was used as selective marker.

 

 

 

 

 

 

 

 

 

 

 

H-antigen types of Fla-transductiou cells Ratio
Phase Phase Unlinked type Linked type Rotel ced
donor recipient i(1,2 i)1,2 Total bil ,.2 b)1,2 Total transduction _
lb lai 59 1 60 £2 0 12 72 0.17
lb 2. 142 0 56 56 0 20 20 76 0.26
2. eux l,i 55 4 59 13 0 13 72 0.18
2. enx 2.1.2 0 6? 67 0 16 16 83 0.19
Total 111 128 2h2 25 36 61 303 0.20
Table 2.
Transductions of Fla and H factors from single phase cultures
of SW803 to SW1157 -- summary of I aud II.
H-antigen types of Fla-transduction cells Ratio
Fase Phase Uulinked type Linked type rote! 1 ed
donor recipient 1i(1,2) (i)1,2 Tobaa b(1,2) (b)1,2 Total transduction _
1. b wi 80 1 81 14 0 14 95 0.15
1. bd 2. 1,2 3 86 89 0 27 27 116 0.23
2. eux lei 68 4 72 18 0 18 90 0.20
2. epx 2. 1,2 1 90 a 0 20 20 »2oilll 0.18
Total 152 181 333 32 4? 79 412 0.19

* Test of homogeneity of the frequency of linked transduction of H, to Flay 59:

x? (I & II) = 6.74 cadeulated from the Brandt and Suedecar's formula.

u= 7; 0.3 < P< 0.5
Tabke 3.

Numbers of swarms per brush recovered in experiment-II (present experiment).
awd,

brush now 1 23 4 5 6 7 8 910111213 1415 {Total jAverage

 

 

| combination)
donor recip,
(phase)
1 112544764 756374 &4 5173 4.9
1 218 8 5 4 510 5633704 5 6199 5.3
2 1367498 856574 7 {f [| { | 76 6.3
2 21957994 5895 67/ f f | 8 6.9
110 o8%)0 0000 0 w&0 0 0 0 O O)} 0(5) | 0(0.3)
210 000 a0000 M6 0000 0 0(2) | 0(0.1)