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TRANSDUCTION BETWEEN SINGLE H-PHASE CULTURES IN SALMONELLA. ----
EXPERIMENT USED THE MIXTURE OF THE GAL-LABELED RECIPIENTS.
Report by Tetsuo Tino
Feb. 6, 1956

In the previous experiments on the effect of the difference of the
phase on H-antigen trarsduction, each recipient culture of different phase
was mixed with the lysate separately. This method is not critical enough,
because the conditions, in which each single-phase cultures are treated,
are not exactly the same and it is possible the different results os
obtained not only by the difference of phase itself but also by the differ-
ence of some envirénmental conditions. In the present experimert, each
single phase cultures of the recipient were marked by galactose fermentation
character, and they were treated by the lysate as mixed suspensions.

The strains used were Sal. abony SW803 (b:enx Gal*), Sal. typhimurium
TMZ (4:1,2 Gal*), and their galactose non-fermenting (Gal~) mutants, which
were obtained by ultra-violet, treatment.

The methods of preparation of the lysate and the recipient from these
strains were the same as the previous experiment. The other experimental
procedures were also the same as the previous, except two single recipient
cultures were mixed.

The results obtained are summarized in Table 1. They agree with the

previous results which were obtained using each recipient cultures separately.
fable 1.
Transduction between single H-phase in Salmonella, used
the mixture of the Gal-labeled recipients.

a). TM2 ---x $w803.
(4:1,2)  (b:enx)

.

TRaecip!] sw803 | sw803 Reci] SW803 | SW803
db enx_ a db enx,
Donor» Z Donor’ | Gal” | Gal
0
0

 

 

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25 0

25 mz fh i 25"

ll 25 at 7 8 25

2 3

26 25

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eS
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B). Sw803 ---x TMZ.

 

 

 

 

 

 

 

 

ecip, TM? TM2 ~~ M2 m2
i 1,2 i 12
Donor | Gat” | Gal” a2 Donor S\_Gal-_| Galt d2
SW803 | 10 Ys 0 SW803 | 16 Ke 7
Gel® 0 0 25 Gal- 1 0 25
swe03 [| , “| o 25 Sw50 6 0 2
Gel® 16 34 | 25 Gait? 19 29 25
e 23 Le
— (|B |e 1 | 95 |-<25

 

 

 

 

 

 

 

 

 

 

 

 

* Feaction indicates: Number of phase-l transdiction / Number of phase-
2 transduction.

r, : Frequency of phase-1 cells in recipients culture.

ad, : Frequency of phase-2 cells in donor culture.

2