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Columbia University %
inthe Citp of Wet Pork

[NEw York 27, N. ¥.]

DEPARTMENT OF ZOOLOGY

“

Maren 5, 1953

Deur Dr, Lederberg,

Altnoush #lilanese experiements voarallel Wisconsin
exoeriments, we seem to be having some difficulty in New
York, We have a pressing need for a minimal sugar indica-
tor medium, Althougn Lilian's old records showed no success
witn wuMS, I set out full of the confidence born of experi-
ence to prepare some, only to be met witn the same result;
extremely sparce growth after three days, and no dsrkening
of the positive fermenters, The most susvect Ingredient, I
belteve, is the eosin, JI seem to remember mention of the fact
that there had once been some trouble with it, J wonder if
you would send us a sample of the dye you ars using, Its brand
and control number, and helo restore general confidence in
Wisconsin metnods,

Also, this lab !s equipved with valeozote stocks of
412, (Would you ssy thet there wes the evolutioner.. eauivi-
lert of several million years between Y53 and 1177F%?) There
are not enough markers, it seems to me, to sfford a striking
demonstretion of segregatt‘on and linxage to the students Jn
Ryan's course, Could you send us !177 or whatever stock now
contains the most useful markers $n botn tne F- and FY condi-
tion? I personally want the latter tc test strain ML which
we ere using, It would be extremely fslpful to be able to
test for tne allelism of some of our msrvers, Or data you
retest ML witn an 4?

ne out very well for me here, I
enjoy the work and the people with whom T works, The job offers
that balancs between initiative and supervision for wnoich

I was looxins, Although toe slant nere ‘s quantitative, ]

find my wisconsin background to be s broader enc firner

base from waich to avoroach these new »roblems than J nad
exoected, 1] guess the old platitude about needing cistance
for proper versoective end assessment is truce,

Things sre worzi

Many thanzs for the reprints, le
fondest regards to Mrs, Lecerberg, the Sears
any o< the old crew that is st7?11 sround,

4, Os

orses and

fse gana my
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