. | STANFORD UNIVERSITY

‘«“DEPARTMENT OF BIOLOGY

 

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PT) oy: + fre) March 13, 1941
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Dear Dr, Dodge: SPM AN BON che REA ences TH Rigg rege ENE S AE Pa! “ “ vt Pee. fg a /
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Thank vyou'for your kind letter of March 5, TS / ot
- - , certncenenpnexsioinnt en mPPt NEA

We have obtained a series of cultures of N. crassa, N, sitérphila, and
N. tetrasperma, many of which came originally from you. We got some from Lindegren
and seme from Dr. Malloch at Perkley. None of these will grow in the absence of

Biokascmxently, and since we are very“ENRTOUS to see if we can Pind @ ste that
will do this so that we can grow it on an entirely synthetic mediam, we would be
very grateful if you cold send us N, stitophila strains you might have available,
The dried ascospores would serve our puposes perfectly well. For our purposes we
corefer the heterothallic forms, but it would be interesting to check hamothallic
onss also. If you have strains of tetrasperma and its relative (I've forgotten
the specific name at the moment) we should be very glad to try them. The tetra-
sperma strains we have came originally from you I believe (by wey of Lindegren and
Malloch) so that unless you have strains that are of different origin, it is pro«
bably not worth while to test them, Do you have any cultures or old ascospores of
helanospora? I you have, We'd be grateful for a chance to try these out too.

We think there is a reasonable chance of finding Neurospora strains that will
grow without biotin since Fries has shown that @ number of other ascomvestes will,
and Hawker has evidence that at least certain strains of Me?anospora destrvens will,
“Ye BRall attempt to pick up local material as you Suggest, with the hope that Calif,
Strains may have superior abilities.

There are a covple of questions T have been unable to answer satisfactorily
froma rather hasty search throwgh the literature and I wonder if you could help me,
The first is the nuclear state in mature ascospores of crarsa and sitiphila. Are
i- or binucleate? Malloch told ma they were binucleate but I am unable to find
sned statement regarding this. The secord is @ Similar question econéeming
the conidis. Hore I have found statements in your vapers that they are bi- or
multinucleate. Valloch indicated that there was some disgreenent regarding this
point but again T have found no published statement that they are vninucleate. ‘Ye
will very much avpreciate any help you can give us in Settling these tye ovestions,

Nay

“e@ appreciate your kindness in helping us. We shall lat you know of any
developments that may be of interest.

Sincerely yours,

G. We. Boadl

 

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