Resome of DNA SYM hess tN Veervous ce ssa Ges
eeok O-B2 cent Nin WM iflae
Sey “Hier oN sais ns “
Anfedhng visus + i “rer “fe mat wo heys
fold bo BAe
3
X¥ Z2x0° 1.68 3%? 4 £10 hones
0-132
ae F/B 24x(0° 25108 5.3410" hy ixiod 53 Ore CY
FF7-¢ufi & Sen O Yori Loxio® b2& ixio®™ fo oes OS
327¢vf, 2.ExP Lynd 2.2%10° of 2108 230 &72 aq
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Fo hue G TOKO 4Z~10" Jaw O-GG Go 110 3,3. S
32 46eW/e H O-4x10% 21 u10% Zor 0-43 Yio? 95 "223 &
zee eT P.2x10 2K” 1.-MHIOY OGD Oqnwe 2t0 “IS 2a
N 444
9997 NP a 3.3K10° Frank Y.0r10" 4.6 33 4
997 N Cw Bn 19% [Tx 10° 2-&x 10° 0-4 QF DI2
FPAINP CS Lr 1oY Lox MvIOS O(2 YO wea.
Nag (72M
390¢v/8 B 6. Gxt bY xio® Frugal 4.9 0» x04 0.323
242 cypE Le >x1o° D340" “4xi0& 6.4 hi xyot 25 4
+970N(B 9 2x04 35.3
74700 | @ Eg 5418 410" gl
FIC /e Voor H
WY Gr 4 x 0" re

 
2.27!

C.j2-H

Prep ef Bsc bwA. D 1.

Sce methos kook -
Pellet oF 2 BSet Flow 60 plakec. Washed ace
Pes.
Maw at rm temp. Add 1 me/e plates = 1a me of SDs —
Satine ( see methods) (¢ Laue). Hort, Wash tn long tf.
Vortex. Vers Vecous, not reaty hamogencous
see Lumps . Add 6 ml ( fy SDS- sate) 1 M Na Teh
DH 2-4. Tne. 37° 30 men SAll some [ight tan lumps.
Add Iq ml. Tras @COH) Shatecr rotey, eae cpeod, Rom,
Spon is’ @ COO Rem Suwunel- Calleet VPP, Ages
Defreu (F ® Oke last loi ks, cot only ;
(ig therefor, deeded &
Washe “Ge (« at | conte ute ) of J¥SS¢, OOF M Pris PHA,
OB viek Vowhey Qo Seca ag ahve:
CoWlect tof Bf 20 ml aq vews - Add ne aox SSC.
Add 42> og COld Grt EFOU Chhong Sido Gf ray
Lecolcor- Shr Weta vock Windihg DNA Cato rot.
Dip S-¥K MN Cosh EY Aloe. = Pagss against dep wake
be dyceen. Dy 4 S mn AE Cm lemp.
DiscoWe LAN Sh O-of SST Lobe Lo bene.
OR overnignt. re Wen .

A
Add Proved Bo Sse Coit) and 0.2 v2 of
heated f w2Q eNeac. ¥!'27 @m. 379. f}X Vam.
Add equal sda ne (3,35 nt) O-OF- Tors, Vark
is) modiom® in PYREY Soxvatt wee» Gar.
OY core: Tale Sup: Cine reennral has Aefrtey 32"
Kdd qual vel 9AIo CHUa to IsoaMmUL ah e.
shake on hand vortey- Spin Clerc cat Conlv,
MIO un £e Callin (2 rent eat Romove sup. a Cal: Only Anveck

 

Boeck of proteen cat iwter face.

 
Place OF ££ nm 4oml conc ae fbe- Aad Joo th yor,
O-( 4 ml 20xese. Add 2 yok ASto. EFM, catd
CES log{ere , WU cad DNA, YnSe cn. EON. Dry LR ACL.
Pot Ww dessoloe m 4 me GOL SSE. BO min On
snake he Refrrg © CVEL niger
Giz} Diatyre VW (2 O-(SS@ sfattnocn- Cold,
lO- 12 FH Cra nge w (2 0-01 SSe.
H-in-F C Kanye (oid -

Q- 12-76 S&e, W380 pm LS onl.
Oorrk + (00 ‘hos SSC Adyo 7 O: 210
210 (/.02) = 10% Aro /t
_ (ft oP Ow ,
Z Aloe -
if 1 O07 Sd /Y owaly (felsene Mh, Guoled by Mert)

a el A - L
gore, pee
ie
Lb? then, 10.4 Azev - SSO 62 “Kg Ju = 0, S4ing JL

—  Or¥t my qe ld.
oY

Cerne

sopg | filler, ret GO fpy Yi L thers |
Sd

Rio Lz QR CO { \hecs

t ~~

ay Pf YO
via oy
| AAD ye vy dd (Uw,
BH eg p . ae
lah gis = ay bike
Gro Hn ef A. in fvenrae Shain (ed, D- q1is,

 

 

 

Fo 7 GD.

Gi2H dso mg MPN ings ml HO, Ia mg Jt N Ao-

Boehvinger Conwot & Ga reuyy (6 ef > Face aced
Pee aced. Cy weeks, ( Cor Hoe Oy Ne P.)

Stable Cold nevlvad, ( Cow neers, Meh Ens tr pF&7e )
Very lable tn cllcak ,

S@ E neo = 1g x10" om* /moe O% hed.
AIX \2 ASL Add Swi HO. Then add 1 Mat
23x 4 aL hpi Gr Ven HO Qu.
Ky 16 Q2Y Took 0,2 & iN NadX. pH GO.
Fe LY GE [N= | hmcteld,
2 « 3, 62 cear
esa
663 MW. SOMd 94136 mmoles.

CO Shaan O, 27 tat.

S ferclere. un 2 lob Amro SOO Laney crta Sheale thao.
ACO rE |

F Grew, bi ko
V2 NU 4s Heart LA Wiccan BF An dissolved ws 4. ULE

deiumud WO, Disperce So wt leach

p of 6 aso wi Hacks:
U- A. : 3K Gi: CS Qe. TT svete ’ ~ . bd. a
BH yur Ze ao 4 Bur + 3,°S qr v0 ge,

in acon dewmed tho. After avlwclave, cov
to Ss? ww Waser bub Add 2S et hanun | 0-05 rt PN. foc.g
J plates. Core. Keop Overneqint STP: 8 aN
Nuweenk Aga, ibe, SFT q+ 250 wh Ko Leak bo bol
e doce oboe: Avleckave: Cook ta ss. Pour Pilates.
Cogn - \eop Over yrg wh 37° - .
Nials - © Od yrycouck

* Cleectrrw by avloelave, (Omen:

 
Fu une hemn-. &
Ve ‘ K ARMY. ~
4 mere 3054 Raten V30R2.
4 | CoM §$ Gagscle.
‘fF ma nm OEY A. HEetnhanclamee WM FO Hf Sleuk

Eatemmoga, Moen Cassoletr IK Lge onc
Isimn 65° Store af ¢#°. coo belay

 

 

4 vials, stabs, inepbated 37% Gash Viscb
& yrals, shrubs. hof cneubaled. No visible growth.

Trowlate Sort BUT- DPN- Hem € ey 37 stab
Throtkeam& For ewe -pePN- Aemnte Ay ase Sterb-

Shake - 37°o- Shur wem:

Pot Ha, as” Stab on 37° mnewobatr . I pme

iai2, 2S #5 Stab- no signs of growth Qam-. Rome
from, Wetobahe.

37° Hey asc FL No cent of pw tin weler bath. Pen
WOON See qyrow A

Neduc te Those 2 and BHU plates mada UP jo-i2 bee

Woreng SME henuin shold lave wen tn

\ ‘lo bre Han S20 RMANE -

Male amt Nethorclamet _5 SOW CT Slenle water:
Blt feu milbpere- Add So ma, Henun( Erk: Fron
Neathars.) Stabe 672°: ve
ae

vo
an ae FA
SW Ante nn «Be "CO RQ aa
See d ES eu ce
Peg Yete WW kequrd (moder >

at

as

  

 
 
 
 
D- 4
PF .

 

(2-7 Cool wmevbated media 230 pm

Read Abs O50 vs WO

Medium Oo
as? Fy O-O16
37°" 4 O1FY-

—_ Ose aad:

Add Hern e DEN TS To wh media akg vcs UTLNRG

new hemun coLvbcn:
#f fF2Q
2 flask. (st medi) wefk O:50 and f.00 ml

[noe vlate
ef 37° #4 CUBA - fi and at). i Dero hme of yedea = O: O6G += Yl tT pm -
Inoclat a 27° vb a — sheb of Na Hans

bird eC. steb Von

Chace 37 ¥ in cdtd roan . Stet ALIS pm:
Plates from Baht Cer); te
Ber- Kencn - OON Gece O-O9F 6.10 6-072
Bloat Chace | S 0-028 o,ay1 0-003
ve Ive nt Chega 10>. 665 18382 OF
= 290° bbe? 6,04 5
ico Caan im sie
20
Shep te 2ar 10° om, Cowr€ :
yar Hy, #AZ- Derse celle.
jo wee et
Cantos nrrod KY .
» DON. Aga? O-035-

 

Medaom PALNUS Hermie me nec) | ON gh
2, « WRLD

Add t mt ef apse at {0:30 pm PoK@ 37° Shate.

Agco: O-O8S af 3) 30, IY la- place at 37°; Kesm7O0oge mm Mt:

O-K. Esgcenhee cetley No Growl «

 
Vom 4 Lm of #4 [ vial CONKCLERKHY O.w re.

a4 eud' -

Move: Viet ¢/0 4

 

fe [Ss vale. frutde (2? (7
from 3p one nek cul wee - of f21e Brees.
Jo vials . Mae a [09, (Doeck Krom Shab )

maak {7-10

Shove ~20° over Reg int
Q
Then neat dy, pohat -60

oa
.
D~-@7t

 

 

For
vy antiholke le
ake Alibnote te
Via -
{
tla, oa
A\4 on :
Streak ,
2 BALD
to pla
- _ tes
7 ; collate
os | .
Dp-(b- Fi ~ - : |
OOo
Go
yo ad
° — al
Net ONE 6
. Cnc A. en ~
ee Aq “
i .
; yer
pla |
ty lab-
Gs
And
Y
qd ‘
4 ~
ge Ava
faln)

 

ee
D-2415,.
TT rats fv Rest prelabeling Con dhans

 

 

 

 

J-\24F!
large plates— d = §&.6 Cm 7 43> Creme T 4.3)” = Se tm.
Sma plates d= Gem re 2.39 nea TT (2.35) = 124 cm™

raho &{ onreq of large [ematt = S¥fja.y - 3.33.

Smal plas, sed 2K 108 Onwy B lon wn G dae -
Usvally + wt of Mediom: GC ml in large plats .

ved 16 place ¢ Bsa Todaq-

QQ-[2- S'peuce. cola.

23- (2.
Kay 3 oy - N xoie- perhaps more

Trvak te long lerm labeling at low level of Syu- AT,
4 plates: Replace mane Meme 1Olo Call serum
SxioS M iw AT (de teqars dT in hot).
2 pli [mt Aal-FH in & 2 eotd:
tne [ot 23,7 Co | mmole

a
3 no?
Yy no “H-

Plan, Fa = Fe poptaco medeom @ hot and cold, Avcpechvely,
After AY bw. Cave Medrum & Coun.

Pe 2O mi mediom,
‘eu MeM
LB cath suum.
r “4 iyo of Oi
O2SAX- Shek cold dT, l mg {l, Yl v0 Mm 42 fimoles [4 yn men

Add 4 nb. b 324. Have Jae lept- ned oyu add. 0-025me FH di,
‘ Count mededmn A A

 

 
DAIl4,
pr.

 

Q32y

2y-

D4
Plan at Go- 266 confluence. Aety ally 7° -& to .

2

beet

lo

W\==

 

Save Ott of FHMT + 4H MEM, Doren stark-

#9 0 Fy. Remove medcom - a place © hotl2) « cold (%)

Fresh medium ee made evactly as 23-12, UsLrg
diluhorn of 34 df- mate 23-125 Ned pert Cr Meme | +t
CS + Oa Setof O1BM Cold sree dT. Add yu to

#4. To Thenadd ost of diluted 34-dT, add YH & Be.
CMelcom E),

a

 

Media te 5-10 |
Fi lea Meme po CS FOr HdT (oper) (ute)

jo pci [rk
23,7 Cifmmele, 51x10 7M. G. Same bot muws *HdT (ya). |
— No label, ho dT C.' Set & O22 1933dT {o. O2Spmote) av
|
Hyer sot F + Or24 (~933dT a?
Jo pee [ +t. ue 107M dh Y
23% C / mmote Died Ge ort j-93-8dt (0+ 25pm)
THOT olabel, co dT PHD) SE +ord 133d. ¢ te

Ate mem,
10 ae J+ sitx 107M f- Cdets hows LYN

0.23 FC. [rw mate

No label, Sy «(a 5M df: .

 

as -i2 Nbr | —>? aon = [ove (ile close &
oc at Pa Mmonolarner
D2tiz,.
p3
(dbdnDlo—per P|
2% o-91, Mil good monolaya. *F may be Slightly lees dense:
Morw"\ Yo Chace on ALL plates #4 S/O.
Stole ane 1 ing [it d7 and d€- want sopeg [4 dT, yo pegld de-

_¥4
fo plaks: wash 2 © euaim Chase medum fren add Yk

for (Macnfenante | (at “cou cecum J,

“Make « ee “AL meodwm
1fSo “« MEM

Spe CS.
Ire df shee cold.
Lot dl Sherk

LGti2- 71. (Tues) fOam:- #2 chil bolu not guile so god, also  s-

Wach eaeh plate sr eG Yd PRS
/~ An FRE.

P rw & maciakn
, Ww | —- Thom laweled plates: 1,2, S, 7, a,

Add eh: Overlying Mediom Cehase jmnediom) | he

ane of plates that was vunatweated, “omove,

un mediately. Remove atl media. Wath.

Add. Au OTM Nat. a per meee

Srnure ol OS rf alkqvok- -To cach, ‘4. rel cotd 2040 TCA. 2,
ci Count O-1L mf by day ine ants GP fo Oe ty Feed. cpm.
Dab Rita «© Won as pu methale:

Dru a, be the &o- Toluene — Levant .

be ah plaks, hot cold, Contos, tre O11 4

ds 4a be Abs. Meadeng, .

 
COOnF dry A lets ww bolue-

Backwound # 2, mnelud cr blank ! Oo §
foo evo't: UNAL ) bbad opm + ¥

Dats,

 

IA

cpm repeal cen coo be
V conve §='S9 S|
4 Big) «= 3120 B06eT 61,300 2.06
L? conkd Q? 22
fF  B24I 324s 3233 6Y660 2.01
Scat 120 403
s- 221,600 2207 22) 0¥0 Grogoo
Room %6 oF
}- 73,360 74,63 73304 §¥66,030
Feonpd 4! ay
zr 125,320 25, OF we 29D SOS£00
4 conte 194 = 145 123

Leaky 16% 134 iy
bf se 34 If
16 2& 2.
ie? 206 130
29 172 iS
123 [Uo Ha
Is iso «6138
Iz20 he lo]
L 1S 20 8

15/308 IAC

2
iS
c
3
om
7
4
4
|3, 500 13, 260 13, OO 53600
[3,1 @ 13,040 {3,100 SIO, 9 oy

 

oe
Is, 200 34

13562 13,27 13 Yoo 53606

MHQeo, ooo BAG IT + ¥49,03 £090" Sisy
JO8  Ba0.056 8,08 ap 0°
*eqo0r es S1¥, 000 Ft4, 00 ane

4 se Wot Gw 2s¥ ge Bs oe AOS A6¥

Y 107, gue

| 5 107,940 100? bg!
4 14,0 Wagtase 14,08 (5400  &e

 

Po
fofal cpm= x 20.

fE Axa
3 aS

Y 23Y¥
6 2,3
5 2.37
lO ot 3Z
Control 2.25—
leat 2.39

tm 2-53
Seuss 2-07
Pomp 2.207

G Cont a.27

Contiols tndecak
less Chan 2 to
Contamneratien fy m aloom
In QU Cases. Meck less
than thaf im come:

 

Coon ts an ry (->10dlo#)
ERY

23-(2°#41 SIOO

 4@* 47,000
a oD 0 46,000
‘Ee YSoO

a pe 51,000
Dat

 

 

 

FH
_ So MMoary - Kel label | GO radchonc- _
ne ee eee eens rene Deemed OTe
In put To ta | Toff Total nso

 

aud A cpm
IT KOO pal S.car cme cpm insef bos ff

 

| Evgermer pe coment FH media: pe Jrumet. 24 dis Peeehan? Cee plac Abs

| ° Seo* & qsver bii1x10" S74 4,1 Lyen?
4 Sea" Ara i. Orbe oxo! Saxiot GO 13,107
3 _ Cx(0° © Lie

Y - age ® vo

° * 10 (0 R10 Ke Gyn O° 3uc10% BL bw |
° 7 Si” r uo
zt + —SK0'? IO anioe 47 6 oxo Ase? 3.3. 4 x10"
. = S1x10” Ue
1 + Sinto® 10 a oe Fe 10% Oc git bese’ ‘3. L2100°-
(0 — Sys ob

Compan Ge q - S.a-: o sy Fr do creates 1OOL Cum SF,

lovf total Coonks LACE PU OF docrcared, les than (OV.
Mso, Wave move cells th £G- gine Abs 3&

Covpgaad & 3L mM oS

Use Sod. Do itnteahm wm Chuse Medicom.

Guctau S len Suyppan Vretooyy

Use 8 J . |
er” | ea alent Wier - 10 gla ee ported
en +o hybordire CM ty
| F hint @ be Uling VEFionny-
a atl (stg 2? hybn be clo plate, - giod bandevg ,

| las?

 

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