| a Bade we. 53. 23-1 ¢ 3- A4-SY 3-301-S¥ Be PE NCSA = Ae Rete = Ceilon le fa brow a Zk We va : 2 . : AGE Lee NB inoc whale 3 iSO rv | loa tole 4 eye media: Own) eins pes Ph) 156 bf c igi dt Ol i Ea wt ds ed PP Oa th i 6 tA th tt CRS BAF ; wt YE Y yes at S % J _ a2 Yt son! £F ia ewe it ‘ _ : ae ERR wa he TO. wv: Wh ghaon a Shak: Am Nye t her Be ue paral Sen Parad a wa. “4 Siclaay hs Lig. ¥-3 3 oo, Fs ope f zs uf. v. “ae ‘fe 4 aM, A ys. SO0 {i RUD Pru f t ip i pm bile wy OF Blan fe ~ J Si Bis Fo } jig et he ava a # we ¢ Ve sh :. A. ; APR sheets he Meda fy tS. Beatin Wor f ‘ pepnreaeiet Le to Lise i oA Ki ae eed eo ig) ws : 5 wy news ont betem vt: sre be ernest VEG rm pa bey i ee aba é Nik et mAh. A bio i of 4 © TAC He ls yo G& 4 * S Co 5s FM %y : Craw fe an ! i ‘ 5; i: 3 st) “tir t {fs ag yeti Veg Me 2 oo ¢ me te RE ot Sy SO HIS i ia % ry ae w. WO ante ae roe pet PVs cAwved. <. a ABS a go SOD ne a ‘oie. a 4 Fee ditty ISOS With) grr Rh, ent Ahad wtlhiign earch Rokk sien OOO ghia | co alee heh 30 j fh aang 7 py i Copy ome Eh) maouda, OP ot 30 aud sce ithe ahiabing x 4 a Aa bprind LP Pm Agss. = NSY4 ee \noculate iso mi media with §.0 mb. this culbag and | I prorat lated YAS ahs cs a eT LY é eh ate with Soo 30 er a shale 4 os! abou ~ > Sta \ PM . Pera. “fe eb GER bs ue a Ea: Ape cteel 4 CSE z A ~- Priekcieais prow wade (t Hilor {BA) rth SOwkh: Thee YTT GS Freud 0" Uo HT thoBirg. Se ry a ay VA owt A hp ig ¥. ¥ uy AM 1, 302 ( = = = ~ = rn fo i . , ; Peeves ae as inoculale Worm, medium wih S mi oft tn fae [oS pm. ? ue uD 6° fit Me vn rt } Logs ¥ et AM } Mbsego * Lbl3. cae 4 Se book ; mye , vTrag «Cekks tt Agua - Pewee Lae bn ece Flasté inguleted /atcy eA ee PM ¢ bree * G29 * oe ( x Z ? wide “ a ih ' 7 ; 2 ) ee ‘ . él PHA UT OKO anKs oF §, *. 5 - 4 @ f { f < ‘ A é i ta % tee oe" ? } : & 4 ? . F “y $4, ¥ A wiv tJ fe ( 4 un } o£ é ibe tne Buf Pye i , vA | j VAT SE . EF iach sabedascinel A actidniihbpaaicen ; Es ’ £ i i ' og ‘ ‘ : ® * Ff 4 Re 3 : : { Ay 5 wiceat z 2 pg dit a. Keb af Co medium (Cadet we j £ € \w i i je? fm } Py GE eo SioGeK. A "Pow , MAM oe “Peep oe A. Gr thE Y Nucléoribe Tie spony LASE, 3-27-o% & tL.» 3- SB + + CAB Reoy Cutrures Conrral wed. CAR Peo y rae CARRY ad i ¢ fa, #2 2 40 Die See = ve j : (pen - Sih ff pin rnga ‘s,f at Ss 7 1% YF j Oe se | Ved | teil) [ee 44. 4 ~ ss = ae i ; | fA, Epa ae Pebtow front ME La 15d | 1,8 4 — hemor ahmpie > Foe ee ee | OF) 2B of: | OB bee. O.D soo Ls S28 507,613 Ne es dba Deauronist Yo bo meorte. Kate tel anf arficide zee eek ad) ee belie, i Ub Noan ° ces Osbnd o en Gt “Dihve ja ioe 62.2) MA) 1 @ | "Begs ae cabled catlaR ca dnd ec bf wll ae. Ge ta sk NY] Peed I . ODoyy - GIP B57 BOS WUE 5; pm Co If nnn, btBe| Fe | te 3) 108
  • 2944 =2O Ga “a 689 >26.89: 520 Opn, le +7. 93> 27,03 * AO Gan. "#2: 635 26.3):20m" | |" 9 6%6>2696 “20Gm "10 = 26> 2716 * 20pm "3+ 26> 2766: 200m \ |*7* 6.18% 261820 4 Ht 6.89 914.38 24b gr FH 84 > 2814 20pm 0 | |B 7.1527 1S= 20, (ovEeRD Tora. Cus *2 O71, S Gow , Cheuk 3 erm ~ Pur elly er pregen; os Page 2 ge Stn ; Proliminary Eeheo oof A. vinelandei hie 4-4-S¢ TWo batches of cells 0 btucne d cluncng prelincnaay tran feat (eg 1) H-2-S§- Ora gm \ pooled. |. ¢4 Yrr-eatle U-3-S6 Gn UE gm: Uomoae nue tb suspend cells gicth UY ml: 0.0; N kPO HY c x (ide wher Coolers P Wash tnd chamber of (0 IC Pa y Cheon Os atllahrn so that tad Volume. a O-01NKPOY Cpe We) = Ii ml. Oscullave 19 mens T2v_ Ul amp. Remove Uil mi. aka vor Os alla iO min mag (20 min) Qa divs Liamp: Col ef. Cortera WEA fo Spend Ser valk + lo'sonicate- U2meywack , ZO Wun Son cates. Protein Ass Assay CMreropro ten). Ly. poe ( Cleou, ad: brows | ; ‘ ho ny bts mo Total ny my o> rie ss te in eet gee Blane mie 1083 Urals a Dacre’ (Album bt orlet) dy | Yop) eealore [Omen Dilute 1-to (dent) .uc 39! 1336) OS4 | LO S106 Kye |. 13 Rc (0% mi) | EES VRE Le ORR ford 20 iw Dilile iio (spr edb tur) YOR” fSSU" | 6059 ha SS Slat Bee Te Coph eed Sct) Gx | OCR a ae 1 ORT tag Achy ity Assay. { mun @ 27°? 2 2 Y . 6 ? y e-y miy, 01300 .300 300 > xX 01M ICH PO 6.350 1350 =. 350 y ont Dowty. yer nak ee - 7 ; x Ab? By r [ot OOPS | at Os) ee OL \0' ey Wack - ei , | 00 ee . [00 1030 1010 eae —_ po! ey Mac es v4 ghee rm ees o , loo ‘030 U6 ~Ver hoanml. 6276 ie 1290 eed Sh BEST) VIS | aa the . le a. é " / £. I Pere en hay heat at 186 Te. 26S 7 1F (9% /60 ape [1 ee eee? Lay Qyse BOS.) 211 Oke 140 REO | 213 O84 00 pamolsl.|ine: 007 «O42 063, T0H 1 3704 36K «GO = P _ ies: 1 OU OWS Fy ; OO eae | J a8 Boy ‘, orehas, gas be Saat Blok ay! W324 1¢ 3 dae a ry ae ie ae a ARN Get 1003 i $4 | ae Ae ys 9 EE SE ag, SB | : | : So ogeeae (a eae Se ee é &. ) ey f£owew — (fF Kit oe po PRyed, ha pinnate BB, feo Bk g, he fr total ane dilyle # Com of TCA sup a #1 2m, plat. 6,304, aOen—en 176 cpm Corr: bur bactemrovad. 1b Kee > 35,200 cpm [As e| Un bah : Te een wh plated ro (1 3 od a abe lane © Ss: fo cpm [me ot un wwe Cs bse" 3. Greenies then: woundow, wer camels ve] | | ee eiak Pwine? AZ 2 Cpr: iNeDP, wile oem joe) : ming: fast. os +2: ss} fem re t : ; a. -, 20 0 ‘ tt => Cpm Cre RS TP ee ee ¥ / wei Bee) cee \ SPO un Pei : Se? FSS on frp iy ic. Pe da Fass (EPA a. abe Qe bails tea ihea go tae AT gs al Assoy Need MOAR. than ein ent Rigel on ot Mas to cen lage UW cold , (PAR UMs 1 bo clear eepeo solu | ~ Shootd plaw yw cre each {m conitunt da iitts a fol. eytrack. beta, “Fein eo 6 *Sr4 Ev dintly an ae ’ eee an: a chuly b oe Ans Ud | Fro in S bbe =. ae Yi tn cpa the +h bas souk nok Ub meg k oo On lex t~ of pre pre : Sk es ee ( Sumpulealay, 5 5d-60 Umb | am mae cee ae oer $y repent oe .o me 00, mt tae rat @ 7 Apert Oud. Garay M/s en KP wy ad a se} Poe Tae =O (A her Tefen se hada de “tlh Brn ll ales we(ic if 4 ¢ Uses thease ates 4 an /O f eC Le ok ci? Pte LA tik conf ey ~A NS Py) ed 2. aad Tee: An. “oe 3b mae a. ( Weta “7 epee f, bE ED woe ro < S es AY Ste chee. C en. Borin, p Sk | : port os ft yn |r0q us - id « manta cells folaced ttre 10 he Cant: ate wd aries Ae oh, > teats JUL ge) | Go Len OO e tie Tae ~Loy, ten. p tnd we tify Te (19 anh, ae Set he iE Cle made oC $Y bb, wth bifffe. Bat ated ois Caltor C pee A e-texl Cafe ie “fle COI taf 5 ig nail fotlocrly ar lage. ate PIE. cr Gad. Mf ef 7 rz go ¢ & £3 5 psy fo 2 Af oe 2 oN x 33 Kefey } ; Sone CE, afte OEE am JOY 3 1 L..— / Rano eke, 2. On? phe. > of - 7- Ar c¥ a s fh é p onsen [04] wef: fa [Neh Diy $Gy « AAA Viel art 2,22 ff Vig Bie: alm Wek 2 2S Ip. (iy fe Soy. pH before Oh, Z Wy me Sey CC mo alr, , LAG 1 ao AHEM CQ MK ere litilled Ht, o) i wee 6 AAA WA, x oD GDB, 4S min. pertedk ; pl udh (4 kom & hug Zo. an ttt fpr = Gi auk, aa 7 Gig Cg, Add {yk 2 VET Un Abed) \t = 7,32 ie ritlian | pee contr fp, The, E. oven eee Hz Afsat iy) Cot. Set tlleetak in 2 «th “TE Ceale, (lea. p&h teseom apt) Aree E16 gan. Chef By adlad, HO Yo p § il 7 Ape Ce H opt. add 3 ut Kod li ae 76s ee ees . on Dies aude, at end. Stir [yor ahdlilcrel oe Sg ete, Ele aoe, Bot Lakes a ae VO AY So 7 Chock Sepia eme ee Cae Py, fH IE arn va whiok pao ee ONE pata pe tls ph te, ie we oO. “4 Vig ; m doth te Gi ky pene Sx f0 ib. oe is Ao ae le AX Pba nF 4-4 sh Pg Gm U cherme = SOidml. = 4-7-Bh gg ay dare Sec Nk gp rre poli tionr, , Ke 'g@-10 VI Gttee # “) Matects whi : oe 2 — Kariog: 7 : ars bso Sno - 4 ~7}- &R (Space) i310 (0.5449) 73.0 2-57 Y if 1:50 yltorp 28ubs Kao) SSS |MHor 6,630 De oy 1.10 WRT 6.632 ; * A 75 (Sarath c+ zituth) TF WL aa 4 TBR (oi, As Fence) £6100 (oil joord) Fou 2.66 Q731 ” ¥ . hisoo (6.bxe tte +2traah) 4s th oF fe OW . ' Iho (osnlitoowe mi) .24¢ AER 0714 = igen | jee . tfieab. : pak cae feo fre gers a Sates Nit ee a Noh wpe acre tenersy nnd Ananes CaS REESE Ce eS arene eee x ae Bote op Velteminahion Ev lack: { 1043 ots | c . ye - eA PAS Sa p's Bic nk. oat ates y Sha fi PO retAPr OD Gi dibi-ia 1 08 10¢ ; Sse 1316 ; ey 15 04 Wied 102, j a p Y My O: 4G G.4%08 G.G4N kt, PO y Be weet : fG- £G:57 . ap gold. ey Ire MG des Awad a ip are a“ orc nett ie Ane . O.07% G.f@6 & we f%te kpeteh Cm core. fw bien d 460” Yolo 42. a sn Mes 1 OF - petals p. fine: AH 19%) > = Ag ee 18% ‘234 - + 737 aes fa Poe eq ‘ fe bs ~ Achak vats = Lak il i uae C£ tf a % i ° ~ wer 30} &. By 3 O, Gi Poe 1G 29 STATS Pe: 7-0 i feel - So aero en. ey gn ns fr Fs a , a f' woder a (obs Wun - Bb ey: ‘ of My wef > Ox6 Os ne 4.4 /0.0 joe 4st 07 ALT. Opt ( oo #0, OG ¢ ore ‘ p ey Ee ‘ % act & ke at Ag. Pa ( | ce dge forctm ) at az , Ce 29 488 a 4 Ue Oreeo 6.3608 O3G0 x 3% ‘ # Q ? %, ie QO 1% vie o ' as 2 “ é E48 ty acok va of A O45 Gi OPS Bi Seem Comma meme O50 ¢.026 bol? 6.100 0.040 gOyo 7 v 7 7 f 22% OG: L%e QO. BZ 0 4G” G0 2\€ OS oo OLSH Oars 27116 ;4so S54,./ 43 C€ 372 ‘Be 234 aos" snore Las” £ i fabs 12 ¥0 2g stat bom {.¥6 oe 2S 900 | Gy teed 7 Oe, 2.34 92 Pe A. agile prep. Sop dialysis ~ [a Ai Stmpt mt Ko Micro f 4 th e - Co Cran i¢ Blank —_ OwSee OC" Qikerng fot Stundaad= Plasma Afhune O02. 0.40 .400 hon Sulé. \ 0.100 O.4oo YYo MT MD Gold | pike 00 0:056 guno ‘rS¥ Achy fy Assay M,o 24ad (Ve : 9.02) { z 3 (0-¥ iy. 1300 +300 « 300 O-01N KH, 00 (380 4359 © .388 A ata hee 6 eT .0F é ‘ oe i 1 oe Magee : iv 4 Ev haere dul \=140,( Tass.) = 10 O50 ins Suter ee WORNes hOow SRS ES aa cpm [int inoub | [94 1U3S0) 2080 pmais Pi meng, 0132 + 296 | 11K A 0100 0.056 Jamoeshi ine. — poem. 223 © [62 ~ NOSob- “18> >. fv] LiNols Heng {sa { my 2:%3 3.34 2o Obtach - SO0.mwL. = 4-7- BE og. 700 = 0.745 bres 4p duly le € “mM - Be Visual Te we ’ 349 ‘OS6 13849 \O0G2 ot 049 008. 9? ot ra 209) “02% be ester 31 Fo of protein : M a Nau (-mye . 3 mt, LMTAss , eH. 18 of lie Mya pm P */s-di ee) “ Vea erm O.6ed . Total = BS USSF 2 s> yy 6 ? = 1300 1300 .300 ,300 +300 X 38D . 3S? 1330 1age) BS AK (O7L 07K OTS ete mm oe “e on w Mon (PSO +100 .0Up 180 .odo XX RE Ea eo 3g SS GOD? DB Bees | Zoey GOYO F130 23YO YOY 243 CMM OL eB ERe ASF 1) O29. 1 a01d action O64 , 026 39% .335 146 Bale peal ia 36 494 Lge IS 7 q oe u-2)- fe Zn €+OH Frachonaheon A. dine la adit Kaly nuclegh de Phasphayia ~. Solo hons O41 M ine acetate es 0-033 M succanate, pH Gd (00005 MV oyclecne). (66m. O20 M ~—D stom: Add hefve Using O04 9m. t yslecne HOE Hy 04). bs ory fiw Toks Vp CIM kK POY puri ¢ 0.03 MVeisere, hae vt La i eee ys nt Heh C0. i )Ge a front) é ‘i ek ¢ ef mrilen. b, ie 6 pad hoe Ae Ui re oy a Diaty ze Oot m4 ic P04 PAR 01005 Mlin Ve com, | : -22~- oe 4% ths et: BR, Arn Salt G4 my | oA: = 30798 mays Dilute [6 30710 9M pg hing bbe f LOH bo fs Te by fe fat awe 4 Sé tal: Abs. £rOH, O:l WM 2nAe -2 d.omum Told * 3%3,0 LEST p mes = fey, o md. Start Tu add Som Abs EtOH: § bey Teer cy, “fi <4 pat : we oS ee ve aod aA At cs een She ok vk =62-& Al, ify. —4 sna wines RE DOP EUEDNA A ae LEA ITEM IE AE IAS pon tenbeiGiay Servet ah Hod’ Top speed, / cor, | ~ I : «% ¥ Ln fold «on —od Me eds (oot? af Ge, the a. bes yuan. aet + gs. . Q f i Piacak Vag 3 up ‘ fr a Ey: pein psre Tebk y alors Shogtd lag 3634 14S or 223.9 * ‘ ris 5 ‘ Z in a4 th a s Elo fa pos 2 peed ae wt, ta 22" ‘i Zu Aq => CO a dick Kgs 4! id Ht a pw 1a Ws ane hea 20 I * b t * . {Qu m)s df is fas iu) - we 54 RAG S Ser Mie Pr ae - 5? e a as Pa «tm -9? ( ‘gb Ant, Si GO eh i ; Ape ae eek al OAPs Kamat ol ote ie Se eel Recs ‘ Cai Rhy: te 283 GS Pl | oe oy oes L ae ee 5 Co a thin, A, LE a fre, rT. Ce. es fa Temp. ry ee at end ee (Hemprepend ys POP ats, pe rite apes o : r : | Take ep protecn of both Wactuns in min, volume Of tu ke- “pe CU tA Solution. KD di ssalued Completely but Farhi ry a mone d¢fheult and wos & SUSpension: Each Wes da & wed GACH SF dea lys es Satvbon roclang dis ly ren Assn PA anes ne opatctagorlt oat! é j i f Sole how, wot; S fat hing at £$39Pm, Gr a3- Se. Am Stee dialysis, be, - 7o mi, G-22-RLR gy Km YY whee Y-22 OR ce. Proc ciws eee = so Ble 2 KK 2% hae 7390 OW a Shae d fla oe Alb. 6-020 UYD 1423 392% (O8 L dilute 1 - tog Bt. ee ti o 0.06% “yd 14? 076 Qe ZY pats IS 7m 0. L00 ae + 33y 263 O42 af T, due 1-sv 01200 fem G6 S47 04S a = 060% O.Too ~ Oty New) R Mc Page qg Achuity. Assay on A. Rafe Prop V3 mi. CN Tris pad £2 Jb el Oa M Male ; es A \ 4,6 G ,00 Achvity As seu? OSM Vevveng, 3 P. * f-22-S¥ Ss eCity Oa) | a 3 ¢ st 6 7 DM y- KX +300 1300 1800 4300 =,200 | «300 F300 0.aiN ern PO 350. 3D BSH 38d BSD. + 3SDi. 13 Fd 1029-8 ADP 24 m Ps x .O7S);' 07S, se 10 2S) OSS OF ts -100 AmSult 1-7-Pg (64m [yy —~ «1 OO 108° Ee she eb 2n-ETOH TE y-22.R, 12 tm [4 fee 2n-GidH iy bate Dry |: 4 — a - Z = dd [6 . ey a = 1f0O* +080 4200. ,100 Hpbi hood Kis290) 228)..300} IS | a2 eps sees cpm pa AE A Sees Lisa hae bese fo — no he: 264 GOH 2 (3Ser Oe ; 6 — NO Sobshak 253 119 3 cpm | 1 wd eneub- "160 131° 6¢¢0 Toul, tom jr xo! Saree [4% Kot cpm / pm Be ' pmol Pe oveb- of 40 (D661 34g Dh Gs iN SS. a D3 ] ; 4.4 ° a % Cj @ pot vie, v3.00" 5$30 178d f) 3 Sex fee IES i Ady My 5 af 1300 48D POLIS — 1200: ~~ 5 bas: ¥70 ¥67 796 geo Camerfrd ~ gy axioepmsd Cocoles) | ee Ee 6300 1300 SOLER cae +(00 1200 19. ,j so 722 70,6 704 63% 7w2O 1354 hs 2360 ago 0 $-2Y4-S& Assays on dn-EHH frachons Ceont) t REPEATS a ae fotrnt an’ Watkiok &. Sursh tuo 00 Bean, “Yu Bek Blank al 1SVO 12GB Standard Plasma Mbvmun 2: mg /u: YOUO| MESO 1, 3q0* 1337 | o MC 422-D Reg diwlel- loo { +100. + +460. | .242% > tS |, 030. 30 x os ideo FP SOOM AAI sD | BS Mock. 2H A 2 proker= of Avhult on =42%o ww ©. 8 7 671. Uw Frachon Lo. Activity t is dled c 60SM Tas, pi ¢.2) ‘ z 3 4 sg 6 2 Y RMep(y-22-e) W300 41-2300 |. 3004, 300 "4, 200.) 306 | 1300 1300 OotN LH, Poy M3 4 3s0. (ese. elo stot She. ato tate Oouex (629-57 a OP 34.04, [nd KR 07S QI” OS fe Bin Cy Ppt te Boe Le a.smg (A O.i 1-10 »O¥O ,040 ZnEtOHT u-22-CR K ae , #0 OSB OO at ae ae _— 24 ing ft: : Dei I -loo Kh ak dn ERM & 4-22 -D2 — 7 fa hy ,280. 18) , 166 , 200 H.0 bo Ug- (004: Aeoa ser aac OS 9G OIE E Lee LIOR ies Mg, Cae ee. é i164 ‘ 042 Wey O5¢ 104Y jO24 10S¢ Tolok pon Po MOP = re (MoPL + 35 (Pr) + 0.06(% ) = 6:06 wm: Total con [Lncub. loy jot posit Pes ix io” C pin - be gid =2h) QGP MED OEE ee Sh ad eal a Ge al ae et NO ORL me Gi¢ DBS . 7649 yt 283 TRG “9 0bh Ss ED | 694 4oo 2r6Y Cc pw 2 ACA fe ee et 4330 %210 76d. 0060) Zec0 pmo ler techangss 02 SY 130° 1449 129 DY 5.4 hd bY 17 GF Geo loka veals Lo Geo Bese Peso “ A owak, w fox Sotft / 6 4 715 Ou cy wf © No: 340/: O. BIETZGEN .;GRAPH’ PAPER 10X10 PER INCH: EUGENE. DIETZGEN Go.- MADE IN U. S.A. ? Page Mi (2,.-E WH r) Cente hge ‘ hoor 4-22 Dee bo p seeed, te ry S Aamo Ve peapital: SW pats Pe He [ent bok ZR [ro nato- Dilole |- 300 (-01-> 3:00) (20) d2m dae Y-12-Desy (282% + 307 4Y-22- 00 denteiWged. 1239 333 Clear SupeAnatant Cloud. Saws ppt fozen: AE ~D - e Ach vity Assoy Ge | a 3 Y R My (y-23-5%) +300 i300 i300 +1300 DOIN KH, PO 139.0.) 6380 18359 411350 Dour’ (0-245) A0P 34 urn[n 107¢ ,078 .07° Vopr di i1-loo foul. Y-220Rey- mg fd 1100 45206. | 31 00 ie U-2200G | dl (-(u° a a Ach wy. Ho & Ver 00 CPa AP TIS, [Oso eee com ~bkad= zi 6! 208 Te tae Se dae: 199 409 ~ WO Sob: 1@4 394 Tobal Cem uncorp 2020 43306 cpm | wood v1, 400 Pe [ weed Ht 40-0 GOs ee CIPS aN bbe 13400 pmolesP /rncorp finceb. is | 5 nie mq. prot [ Wed: 1:O02¢ 056 .0r¢ MFS 4-22 -S5 Serva, Angke Head: Sma preccoctare colecied, Love so penn keuw Rezo ome < 6 1300 ‘Aero J ‘350 1 3xD we / ' OTS: — ’ 1200 1200 Pee pq i2 he mor) ,O 3M ¥ { vd J L744 a7, YOY ux 5g%0 (1:34x 10%) 3s OSV i OS¥ 6S Set 73 1 DOF ae OY 72 U9 WD 3574 Crna Cowes 3 tule, Gee Protein on centr Wye d Y=27- 02," Seng HO 00 - a Myfumpt S/o Bianlc ; _ S06 10103) Sundar d Plasma Aib- 2-€ mg fod O.b26 “4 $d hott done juve qyezy figure: 4-22-Dda (ean) di-i0o 9400 Yoo 1234 1176 029 29 Sav AsO *ZOGi! 3 OOH Bt Li Bae OP Ce Sb Naw 2-my a of of Smio 1M Vas pd t2, US mM On M Mall, 0-6 a O05 M Verte ne / | ©: O-6nd PS” (u-22-6 scp A: /et ) # 33 a Mo > 9.0 o let Pr Assays 0-300 mM IZ - my, 0.34 m Pe (KH, Poy 0:01 n), 0,075 oe ADP eG ls ) : mys ( aupm/o) enry Mm, H,0—p /. OOM. (Vel ~ Bn 40 = 60x) Coun) q-22-ORE, Un OH wimg fl, BF 22Y mg. doG 224 wm: by adding Iie O:01W P04, pH? 4 O Ok nt. of y-22- OR, _Crawinh ued ; Add 030m INNAc @ OU vss ( Chl phengbred) Some mec: furmed . Add OrRomt 0.24 ml CG 3 (Poy). get (Segna Kot e-2vo)h22.4mg [A loves zr~- # Slowly cold. Shake toma. Spin lomn, 26 13 OGD pM, AN6bOX 4 (Lovrdes) - Soper = UM. emo Or rt iquot- Pot'r - Sosp cn 0.2 m OO. M KPO, pHs, Add Ode pub qu as abo, cen W Ge Sup T= 24m Ppt - Sospen dg ml Ov KENPO ple W. & peat qt skp 2 WM ar. ( 014uY wl yt) Sop a e Siup ~~ ~ $7 Atmonr LOL oye .-- Tore Vol? 2.6nK oe) ots eae SLU Pr t 1. - Ppp o- Sur ot Khby yte.0 TO ELure. dewr AMER a BON 1 TT Repeat, (Oye mi. geld. Lek stand 321 mene aftr Cemmrihguy | 271 ™. Ry ak SU petnata nig as Be a tebule ©, Eh) 9 peti -~ treated as above: (ism) Tan Chanti fged ) ; ae ‘ewaks aad Samples of svpunafanks Sforcd Joren | CLL sopenalants the fn o one War hokf ¢ear: aontacred ? some pe er pi Fak EVO Mon, aay IQs Peep. CAL sins Risk PATE” Ge. Susp. ~ SGA, Dag y (3 AT A hw j Des plats CAGLSS oO” Ged. Mille Git mt Pont: Lon, | =a |pat— |pmousll amotehe| moe ere | Oe BE ee red -| er gotinel) talon Pace | Blanlc pOGR ecm | onde SW | 21S 9.200 | Qiaued dd ok $100 | 1060 | sogs | N38 | ee Ot ee be bebe ale | as) WS Se: oa eare 1640: Sipe ey | So PSO] 189. Las eg she ga = 4b . oo 22. ng ful: | moles 0? ov eh: protest ever | nh err pw Fever | ma or Voto unk 0.0. a ent 14 oo ee rag $22. At eee 4 2 Ws Wd He eRe AS Se as 6 m4 137 eye pry LE: —s 23241 ONTO GOW G-0T7 124 4809 Fed Ssh (ae 71 4 Y 17 z : vised — 012.00 ASG 450 ,16e d. 6S 5% 344 TSS | ed bY 2.78 ld 3.54 Assagqs MN Parle mi Kary Ce (F04), yl Skep Meimog g Unies S--SyE - +0! 1.00 (0.994 Ls 0.00) = CAE se (40 Tes os) i ~ 3 4 $ t a ogs 0.0¢ HO b&Vp-000 900 oT Oto 0.160 087 © 10 - Mig (<-s-<) 0.300 01300 0,300 0.300 30 300% KH PO(Om) 0-340 0,340 01340 d.3yo ‘340 «340% M00 34/4 bof: — 0.0796 b,01¢ were 1015 ~ & del \-1o0 U-22 DRc, cen. 1200 6,200 = — or dt (-25 a Au sup © 6.200 0.200 ddl i-257 Gel sup T ‘200 .200 x(,0.-4 SO iis cpm ~bicgd l6rB G06. 1270) BM eee) PORT — plank tos S70 259 qe : lo fol gpolne. (nea: £480 2S / 80 Total epm/ine. [14 oo cpm. Total P 2s (Avpe)+3.4 CP)+ ot CP )= 6.00 5a. PE 14, obo cpm | pemole Trorganie Dy dukin on Tod soe. (0,05 a4: aliquok ) Chats Sub) pr ake olla Biter fs 4h y Assays B-Myals-<—> xX 0: 200 ee 6.01 o.60 (0-49)” 161200 =~ | (nos osy Covey - 0.200 en (0.05 50. ) Coacy” me 0200 a) aos 20.50) (v4 i 0+200 ~ (662 > ayo [ose] ” s - aoe 0/200 pe ue > o50 Loo) OFS LOCC OCS 4h ORS TOCSY Cee mee I Hoq WSs 498 23.0 J2,6 472 Jee GOR) OS MSS 2 EL te Ue TO Gu70' |} $240 (522 | duo 12 See 12, 000 cpm [ineubah'on. = 6:00 1%, 790 cpm] peete 2240 \Yyyx 102% 013, 1007 1027 Go 5S fH 0+) Ov) O B Gil CMe He ee a ere 4 Mork | OE | Se | bl 20 Jamo Bl. Stand : Ciel Sep © dAli-as Sop im di2s Sup i dA 1-20 Sop iW def re Elvate £ di-so E luaG iC di-w YT ive . WwW db {-10 Sup WT + Eloag it = Sup ® + tlyak W os Page i6 Samp 0.020 0.075 9:07F +100 0.200 0.280 0 200 0.200 ©O1V00 IV sh le = Prolecns on FWrackhons Fro nn Parti Minary Ho op. ~ae O00 §=— ‘DOS = o-4ueo i410 .3u¢ OUT 2S. 160 YU | ER AS 04001447 | 0.300 + 32% 1257 0.360 +338 27 7 one RNS, ‘ (258 43 So NI 2A sita Vced Oem of 4-22 OR oe ain. F 224 mg. Sup + Eluotr = 4i¢1% = Squnits = 33 onik ies 24 - Grek mg prob mg/m ——__dilvkd me «O86 »026 1630 i016 042, 1O4GY 046 103) 1014 Sup i + Cloak me bho + Jyy es UZuuk =) GP4pOwW « 2:0 te) 0 ee alee onk = 2¢ rz > 0.38 0, 4d OVS 0,21 O.v4 0.vy Olt 0.04 Fg pre k fi4.y 43.6 42 = Sin HS S-6-¢ MG Ju To fal Uadd Vo I. prot: SERS 1010. eetten, 24 3.6 26 44 (GS us oo te jor Orsi 8 POs NT LG 0:3 io 0-4 ors 13 S28 mg ) 25.7 mg. 9.9 mq. 3.) my. SON oMay 19 SQ [MW Reerare Bur ree, B50 mL | 2M |Srditm Uetate -30-5C + 2eo 1 | | Hv ~ ph< 6.44 (24%, Lk LAT He Yanai py. li HNe - rpms 4: co | 4:0 hig da wae 1 & : | | 2.0 me ere eal touk for bl st 1 a ee | Omsk | be os 4 ee te: $0 na : TSO Wak * fet pte ch Oh a S| ed “FES 2 oe bh hee oe eh ee Ls a hous margin i pn 56g). + 200mL Ii Me Pe £69 o¥2) Vn 4. 67 (v¥2) | 2 On) = 1M Sani tea Z 4.67 brt2). eens ovatadend a hebithans abort a pH 5,08 S| 3 / pete ee ore TERA SEO | oe 1M Ht Sn fl we 2 oa nll | tM ile = Aik = SYP (rv) ef [Ase é 9 a Pe Sin Pee Hutte 2M prskmmary get Ste. S -6-5&. OY m). 4-22-OR ey (ee aw.) ‘on- EtOH TF. 2emg fh Oeml= 226 22,54 my. diivle bb 22UM FT hyde groin KP Oy pw 2-4 F OWcel IM he bolfa pH Ue? (=7) pH 5.9 - Prom Utah pamphe, OOF wh ha A me 1 pA re h a - \ solvhton opaleseent, bot no Atal Ppueapibale - Add o.2 wh Cay (P04), gab (ace S-S-6¢). Orres- C4 mun: (ad ding gel Sher une Un ice ~ Fo fork th pee Jhwiis Comber begy as $-S-SE. Sop c= 2 wh, Qorm Amoved- Caen. ~ pH~57~ CP Gel r - ewe & 01% mi Ou NKPdy pue-o. ES oth @ am. temp, mn G| (hy, Aigte Ta. Cenkihas, dtcant). Rrateluh'a of above & pH74 bo fa- eloak the) r Se D+ 05am CalPOy) G2 ~ Grin, Reb tw ter Z cceereg eye ACA) 20 ctr Sup iL = 24+ hes 0,02 mM Aamo ved Koren pw 7 GA I - elo Hw ee, OA Ao ve, Wh 0.1 M P07 piled gyre. ’ ¢ THH- Sup. IT + 0,48 wh Cs (Poy), aol ~#b i, btett ceahous e Cy, Sip a. 727 ml >re Saued hosen. PI~ SB Gee Gel (uo elu te Fusveg QA Gdboue- Stow ct fachows Rozen: Singer oe sree & Assays on second Gek PAs (Manan y = 3 q Ss G 7 in /o road R- My-s-cee) 0.300 —~ > Tas KH,P 0, (o.01N) 0.340 Pi ADP se ph | ol 0-02 ee oe ae SupT di t-so 01200 ee ies - 01200 ors Supt qi 1-5 0.200 ae Eluat Tq dui 6200 yee Eh dwi-w 6.200 ha naee ip i-so 0.200 sia Bb _1-Se 6.200 Sn ® ijn 3-5 0.200 ve) Wb i-s” 0.200 NG4) ho des bool Q.060 085 0% WES OES ORS .OXS woRe woKe 12ESK | Eprn— boqd 263 x" [ol [or | 132 Deb QS he | Ged QUT’ fled — Coatrol 216,°\ "Fo qo i iSO fF COG 430 Loe CT tod Tobaheprm iacorp, 2330 9949 «(060 1332/66 ggs0) 472” Yoyo Faso Toft pr [inobah'on = 100,000 Total P hii se Ot PORE POob = 6200 Set 1b ne cpm / nmole Pa pemoles pnesap fur ME 1054 Leg” (OKO! OfO. SAS | 528R ) 4S Sa pemoles vt: en2. 358 5:9 [+b 10 2S 149 0G FUR 144 imo] my. paok: 267 hl Ove LG nt 21 FA AY ea 3. Total iit IS | IU PE te Oe a a Lid 2k 0: th8 +-22-DRe * Rei | we e oF ve 137U, Supt + El Ta + Ib = apewaein 51% units Ba mo Grieg oF pete o. ) © Sop + EM Masybe IWS Td aU = 713 on [ee my (130 F2b+ 3.0) Spy + Ola 4 Ube GO U4 = IS unk. 44 by C334 44 cad 46% o{ ons los& . Page 122 WED 7 by 140% S/O GER Az. Eve. Petey awn) @ Teore 08. Mien Ob, Th) EF Fer or pH AD IWS IN Gey STEp - Se Croleine- Phew ~ fil ie: -6-S%, Piciatiod ple ee o Powe hay ab, (apo ing all ae] @ |SupI 50 BOO PO a8 ISB ,0263 1390 21.7 Q\Sup I 20 Bl 40 299 eed | 0381.) Saf 13,0 @\ wpm Ss jo Ho Bay 324% 0938 247 73 OtbndeTos 1:30 ito 32 1259 187 .03)] 7% 2.3 O|” Ly ro pe ee 1237 107. | cage 7.0 2,1 @): me) 730 50" sp 1208 |. 208) nab s.9 aul QI)" Ty 1:50 “ye .30 310 240 2375 10,0 3.0 Gi" Fr ee aa as 283 ABZ 0304 3.0 orf @D TTS VS es Zor 232 0286 39 Ld OVA Sangh — ou!) 4g 315 927) 337.8% m4 @\ " ee hg ae ee Sey Oe ee ae 26) bere a) Bets —— es | SO ee ; Used ofwh +-22-bR eo = 22.4 mg prek +737, ae ee “DR Er0x- Aleng whe 208 m rctor, ah Pe LAY md Ol Keae eee @ Rewond bl mal Mdutek tregepres aud 2d8 1M Oar taht pthtkh[ Gt’) 5-05 : a Se esa. 0.02 wh ~ gl ~ $3 -b,0 + ©.05Kf - ph Bib “£7, | (by hcuskhes cap saris GR |Get PHSZ (oy) FPB oa p m6,2) oD mQ- pH~6, (sor mh- pH~ S.3 -o.00Snb-phn £9 “Brad dul = eb Swe.’ SeQck= Canali Z ph sia Aide. wae Bealls z od) ow moter while, a (usted pa~ a Z0: Assaqs a eu adj ust. ee pert werk ee ee ee R-they (c- 2010 547 cig. ee? 5 oY S4id 6760 He 5540 fara pe care CCE NLA 7 2310 pnots Pea 165 #561 Seb F454 etl unk W686) ASF 186 137. Ol —> Lov (0.44) (Oi 01300 (0.29) +600 OL7se0 (0.£8) (0.x) 02 4 .G00 Se Ngo Nelnee. S-27-S¥. Larcp Seale CaalP0y), Gel 4-22- Dose canteiuged 2% ong | ml: iGSuf/mt. Cll chhase Megreh@ chine), font = 20 Mg prot. Ddvle B 2e mi. with o-orM KPOu ptt 7:4 17,-£ O.oiml. aside for ASSAaY+ Add iM NeAe, pH 5.2 - 4owh > phe 6.200: 6.3 wh FY ny) 5.2 uh 5.92 Gr) $6 U9 0-2 5597 Gos?) (Remove 0.01 wi: Gkavot) . Slightly Tu bled: Spin PhS min, 2,000 Tem. 21600 * 4. Gliooy dec). Sinalk gkahinovs precrpitotr (04 wt ok. vot rat Supernatant) APD 2.5 ml. Ca, (Poy )s ehh Cap; & xg [mat ) CHAS mun. Shr zo min. more. rH Contes Was 10 mn, 13,000 rpm, 21,600%4 CUE cst Sop C= 14-Gmle pH= $8 (jy). Bmore 9/02 ml: for ASSAY Ce Cel C- Cole © 3,75 mi. Oy M ICP Oy | pH 6:0 (s Mun: SHieveng ar fm. leap, 20 won. in ies) Sahoo we Qs above Fabs es Woh Bet Lek ‘ks i Or) M, =i 6-S— 7 fo sep ft add Se mM qe. © ver San. Sfir 20 Mn: mar, Contuboge as Abo barccbleg he Bosal a. 2h 6O xe Sup I = BS Hon’ * p= 5.9 (28 es Cad 104 Geckaite Hys.2.- 0-2ul {i= £79 (14s). d. 1m PSE (38). =] a Gel WT, Elvte a above. (a1 M KP %y pH 6-9, oe then with _0.01M LP pH 4 32st © Ip 3, 5h-al yether then with de M KP 0y pH 7.4 80 CAAT, = Zbu)~Mone 7m? & Sp TT after di pirse , 2. aBa&b Sl gL oth Tn , Coat ole Onin, out. ae aboor So pM = ies Cn ees im #02 mek oa teat oe OHS KENPO @1 44 KPoy phb-o , 3.5L) =f Te -3-B rk - gellar, aod Re ee. ori KPOY, per Y, 2.7 oud. lat, ~ 3. 5n8 - qlee t S22 Rite Vreaue, (Pew) 59-38 CD PEST af raat. ® sup I ZB Sup Al @ Sup ar OQ @.Lu ® 1. Le Dea Qure Qhe GO 2 Whe QD <2 my DdILUTI OA) Sple 4° } = 30 RIG . ae i! SO ov 40 i; 20 et (eae TS i:.0 ‘ga +o is2o io! » 4o i; |:/e0 MEE, Lo 120% 30 Ea “ao! a 1/0 NO v Yo 1,70 10% eo sees 0.01% HY ae O00 44 i tae wavs aia ,50 deco —Bl rosy /ebigien, 292 bea 20222 oA 15) 0236 204 = (5-9 0249 oRag 270 Toe? Lou petty oe’ maar 2'¥0 O21 9, Ral 21 .OB46 Sens F 160, 9025 ASO ~190 OLd'? ey .2a4 03 “ENO OS OeO) 088 a VOR, ws ~ "O02 BED 5066 : , 060 ple 1D ing So oT 439, | 1s 6.2 ek 3.5 13 \} iV : pi2e @ Assays on Gei Step | (new R-Miy - Le ot ose e eee SH si G Bee 5-4 5€ QMe G.300 Ee LH, Pa, (o.oM) 0+ 340 % ADP ayum JA 0.015 7 Cente di (-(co 422-DR cy 01200 © i- 30 on eee 0+.206 pMadj.|G-22 me, 01200 Sve r |dui~so (61200 SupT] di 1-20 012.00 et eee 1/200 {- 20 El yb 1200 eles ‘ lew. 10K 2 jzeo © Vr : / ope-Hiniz 492 497 S00 180 133 Jo4 46 a! ~Conkets A eb ag pay ee ag Tofaldpm wc. S'®O 5230 5260 (420 1230 412 209 Total Cpr [sneub. = 40, GOO Voter| P Tilsen * 6:00 Sid. P3% - \$, 200 pmobs 22 sleep. (Bae |. SUG) 0093 1b 1.0C1.| 068s ord pmalds Primed PO Se) | ShF a he ee GO 4a. Jmol: 7] mg ene C-like oy I hg im 32 Total Goch Wd RG 2G) Wes eee eg forall moh 24026 FOS Beg INT Pe eee Totolumbs (1209 1459 160 679 ags | ehh i Singer Heimer S-F Se 101 > h.00 (.94) 101 —9 0300 (. 29) 1012 O00 (+2q ‘o2—s00 C4¢) ‘02% «Yoo (.3¢) 102 2 -4oo (+38) 102 — 400 C39) jo, ~> 00 (+44) Asays on Get Sktp (fort): 2 Sin ee Fhe Coe 3:6. data, conti of : wel! Bo gle gl4 3 F fe 22. 1) Q- hi OabQae By 1H, 904 (0.0m) 0-340 4 kor wana 6.098 aK priadj. j-30 0.200 » O(—> -200 (.2¢0) Soe i-s 61 2to >.> (Goo) EL ie j- 0,100 01 2.20 (.4q) Et Wb |-so 1200 10f —? .Sd0 (:49) Fl Rersa ‘200 101-2 S00 (4a) Elig '" 200 Kol ce Luc ZA US a ‘20d ots. (65 HokVel 08¢ > 2K Cpm- bled = 7. UD) |) POO) Sa ee ree | es Ont Bre Stontole2t | US6S TP 4 Ze 4s 7S 11) G24 $¢4 [obetcy Jno SOD 470 3Y00 G2 220 Gila Sue 4 pmeheorp 331 1057 1230 054.696 1 594 «Heb pment tan NG) TIES TIE | a | AAAs a te jon - Sg Jo ee Fy qf i Yo IoletiptdGia” | Nf ueid: lS 6 ae se BR ae Tobit prot 2%. \20 YH 2hT Uh 1313 (WS Toballdnitee: (460-1 66, 4 NIG. eRe? | Viet ONS ory Mgpiok units Kg spot woul mame pink Sop T 363- 670° Ger 177 1 2%S Sopp zo bo tle ie 6 ate re Ui hachnd4 TO phon pee > oh dy dS Ce he | See BL TE Bu tor NS oe a a BRS) 690 ole | at) sz 247 Et). 48 | Dae # Lg Aepetal Leute Sup ae fevels Sin p24 ba | ot Assays on Ge\ Step Ceont) 5+ 12-S§ » Yo. be Sve, assay is_ tinea e neve. = ¥Y¥ S00 oo | ay tot Se /4 10° | Peay 4 Me; 6 i $ q Q- Wily 1300 — ? y Ku,po} (didis) + 340 > Xx ADP stwmfel OS — of rs tee) OT Sup ese eh aa Sp ee es os ELE @ I-so eee ae aa ae +P 1200 = “Piccutus) U0 & 100d +255 IBS oes owe ee eS uo yee 128K beter | cpm bla KK [22 2 oi 613 279 339 143 Sob "OE Zl ~nho ene [21 247 Sg 7S 31g 122 © “nolsub. €7 pee 525 744 204 3 [bp we: {50 pote 5¢sO 8250 34u90 1240 jam P' thot a! oby We ES aS Qi 2 hae prPine rian 1h ME et TAT 20 a $a SH BS Le 1 nf eT [6 (prokern: 4 26) Klo Keer ar Fete ws (Preznsou.) on GEL Fenervons or S-¥-S. Jéig BerrER —- DicautipNs rw AiO. Drcuryy Spee AO. CEOme bet é e— at ee gaye Mon. 12 amy 19g ny fale, gal nS 2o6N | 4 | 0g RR 2 ON eines Dogs ike id ® fduets Wea, 139°) ee PG Cre ee “ ‘Ge : Oates ry BO a colt tb 2o0 ..0 Raa eS oy 2S 1 {37 iy (Be 3 7 vob F_ [JEP 1 O3H | 6 | (10226 2.9 A27 | 0213 4,7 i eae 077 43 | Te Ute tte re ONY S$: eH 0188 %.37 oa t 4 ty | 1:10 | Isto | 1054 ee 1074 ov 46 0b" iY bel oF yee 43 |.127 ots | 007% LYS) 064% pos sot 1, 178 Cnet CaN Bas i S7 | orbry agi | om oe sae — | ob!) wt Ce) Ova eM he eer __|Ao cking ao dia Auzew : staid she fide ze | ROP AW Shy OOS 0g er ae es | 27% 122e .037r | eo | ut 34o~, ong be ee ee ection enim ener aa Aung aCter jo PM: [sol ger te Dighes es: sel geTe a. rh “daaind hw wn pee: oor ee KH P04 0124, buh Sturt § PMs Rocker | | ‘ Dilys isteped | a ei Ta 3.14 mi. | = ee | eee Re to | Ne: seas eae er eee ee ae 4 Eee ek a 5h eee Be SC POSE 2B Nek aes ie ba os ee : | oe ere tet | ee Fas i en ed oe cL NO Nae pea? ee a } | ' ; 4 p 24 Assays on Dealyred Get Eluates 25 3 4 s 6 os & - My 0: 300 ee _ DOIMICH.PO, 6.340 > YX Ape syumid 9.095 oi ioe kK ELT Dial 01200( 0.100 [omy 0 | Re T Agen = 102 9 00 ELOb Dial? = Cale te yar 11 2 100 H_O COXS: | AGS |. 238 | POS | TRS, 2054 i 4s 120 KK Total epmfine.= 75600, P60 sa P*t= 12/466 cpmine Beye 473 197 82 YN isg 2.0 RLY — No lene “7 19s |) «4o Un Se. Sey “ne|sobé | HS4 1.33 hae UN Ae ad Jokebagpn ine. 4q40 2010} ¥SY. Ysbo (720 pm Aine: ggh* ued 106% ,362 136 pert [hen q¢ 7¢ of \g.l 13.6 prot ea a4 qo , 2uS} (es? | TROT yavt oe Sta Whee S-13-SE Tne IS May" SK. VPReTE INS (Preven) ~ Diaryzep Ce treps re paz? 17 3n-& E7 OK Feper, + 22~R. Duron Spur aaa ~ Bh ee ong | il i #.22-DR, Fret lii0o. O41 OU i aaa Lee forge | 2es. | ee “56e | P oat | OF ee tne ” a i. lo. -2.0| .30 | Se 266 ,0u” 24 zx . ' 319 ; = Rie oe peas @\e re a | [oto Fo ie “4Y |. 20% IY | 024) 4.0 | } | | jo] A : | oo £ ees Ole folio | gabe | FT OB Abit | | | F; Tea! 1 or Cee — Dit HA [P73 | Sd ford | LS Ort tle. oy 47 Bos SB 2uy our) ty call, Ley | : Sta Fane [od e 0.0)" 44 eee ae | @ Ace re 6.0L rug 383 | Ean [ ' © Bo ui- ee sl ee Ey | Do « | Pe) | ee | | | | | | | | fee eee Ne 2 ee | | | : ft ed | ea | | | + | | | } | | | | } | Lasenses LE | te | La Re Shy Sad | poo @ t ; | | ; ) | | he thee aes | Ha Ai | : ! | Mt oe does | 1 | Singer HiLmoe pak ah §-/6-6& GEL © “ lO m! 4-22- Dee centr. (2¢-my]o) Jo m= 240 mg mob. alk & 2e 4 © ooOrM IEP Oy, PA! containing 0.0016 M Cy kine. Abd IM Nate, FHS Hoy ~ prs 5.97 (132) Toran | iG rk - pHs s.70 (10) Whe forth Peoaa Le SEE pg E429 ng [me E14 F mg prof /+t. Abszeo 1S Avr 25a as Woy), gol (22 8img ld) Cree Cruse, wren (nay), Onin We, Cer 10 woe \RoPodmne , 214b00X%¢ ~ hour nes, ; | Sup Ll = 29,42 - ph = £74 C0) Rnsdiis ie No: 0G wh (@) pho. © m2 ed 7:')% = 1 260mg /m Ee =. 13:/mg pret [rd gos ok E.unre Ly T- £lo@ € o937cnl o1 Mm Nel KPO, pH 2.Y. 20 mn: Zon Con hy huge /o men; 13,000 Ape , ak £00 * 4 Chis indian. BPO Tae t ae Ede ish! Bash ob sua trv? 7. 14 ZY wg bey s 3.6 1g [at Tl B Supt, 2th 25m getab there - oa Sri, Cru¥ Mid 201d. Contecpige eo Abtwt - Soret lh rs COW ~ (Pee s20, Gypr = 802ml = pHs 5179 (129), Romene 002,225,0,Sal iple dill 019.00 S00 * uae “Ws 234 myld = 7.104 prot let hind Cel ee C376 mM O01 M FPOY pH 6.0 20 mun. Cas opie cea \\ \\ M ‘ Vy f ; Fino «200 1692 | Gus a er ee OS -7.50 Ieee me ue Mag = Pes |e 212319 | 4 5 BEE poll 7g mw age tete EW hin nly 20s, oe S opm > 36, Oph “pt = $9 (119) 6:2 m2 - pas S72 (19). yi ; w onsase ea RUG E 73 © (Tang park Ee al @ GerTt Fomwe Erin ao, Koy pPwb.e - ta name (te shows) En, Ty * SiOnd geet 5S BwKO./s i pagt = BShainy, WFD (40-6 2o0)Ft TE : S.Om\ vn” " SOmk o11m KPO PHA ~ 6420) asehene LIT: KBmb, C2 We vg = yh 5 OU - mare, crbiCore ea } fA —_—_—_ Ne ‘4 Seg TT, afler adl pht- asf. , Ae abaprs.~ Cork Cees Co-t¥- alipriny for 25 ne: Ce 2, coors leurres®@ Sup dit = #1. mw - ph 5.49019) Ger lf - Duk «Som allt Po rd 6s 3etdon) Ths t ob. - BA? Ror. PS se “Rate — pare) 0280 Aaee' “BYES Isher, "i ry aie BIR AAMT, Roe Swi ator OS £0. TT, oem 490 (1.377 708 gee inte 43Q e lary ost 1.959 27UD 600 an- roVe-740 > 1:40 £0. gai! - maak. “9 3B) ey abe NYB- 399 2,079 oi4q”” Suprit ts ww 412 G00 a 04 ,632~ “tyaz SW Y. ver 2. OS 57 Gob Tey a sTG9G 2°) el a a ahah A | : Ee Tr, se eee 400, e, Sane “artG 26 6° ~ Y= IRL * ise ae 2 rg oe if 1 00 658 re ce lob $30 ait DI, cies A (S53 Me AN oS Te Eats MW a HS rl mee NAyon- — ot A Ae ee ; o,05 wh : ee ve wv Nd OR AG Ohne ee Rio. ane J , you AG Ot idi’ ytocs Te me Ave ? } 0 OZ wal ¢e i OMe ‘ i &, Otnay cor QO uae, 2A SIGE : 3 3 ILMOE @ Assays- Cel ee vA $+ 16-0 O° Ney D-Mit gher tO Tees, Bez 1S eh Opt Oh Mg ety, ge euan Oem Veicere P32, 0: 10rd Chad Sy se no corneas) + & Eo O- - hope 3 Ly g 6 Total So /o-S¥ 7 Se Jokde Se a ¢ pm fine ee Bemip | gbe ; x he yoe 0.01 Ie, 355 K P=60 Ape £ td 100F re — K = /¢% Yoo urge Acer pe ee 0° — — irs d00 (124) Sup xl: net 1200 in he ean agen keg (ue) fan r 1 B08 os rss Te. 0 Cuc) Sop eee me ~ vora.co Cyr) to ae a f20. 70 LEO0 2 OY PND te ead Ep 1200 ea oh 10S -D,50 bys 200 ae 103% +20 (an) © {0d O75 7 { iOS QS iD 7s” 102-5. CO ; = ' upm- d= db ate Dee. tan 3474 tO ) 30 {il S69 = Convo | Wa7 1 G4.) So. | Bom, | uy Lee ee) foe Totaleper inenp: 0380 S710. 406 Yoso 3749 264 Iso pmPures 37% B46 dak AKI 1026 gig oO pm Pine. frmben. 55S ae eG. Bsr. AS ta Totalvo!, 246 24-4 ae SOB Bib | Bob | Bee Total units /666 [450 #O6O 1060 SS 3. tyy $.d: Hive 9 ie. S14. bee pre },0 Tota! prot. Lip ee love 197 Gee te O 144 Awsays - Gev. Srep - poge 80 9 lo rH" ie 13 R- nly F 1bS3 6.300 $ DOM MKngPoy 0.340" X AvP HaM[ml ©. 07S eo _ tiie 0.2.00 b, 1-0 D-L0O YA C2, 1-10 ero Sug Ty arr 6,200 ce ae | 0.200 boby.10° oS ik Sa Cpm-brpdez 314 E96 ISS 1 GOt 28 = Conkeot {SOR OFT Viegs | SSS © Ulododm ene 3390 700 |y2o 6559 2170 pM? dae, 236 $27 099 YSs ISO pmPukfacs 1% od WSO AY IS Lidie | SS ag as Cofsb ones, 620) 13% | 20 1 ey 1S S.4 FOS [AO ae Set Sey T otal prot: emer p 5.0 a) ee SnNU GR, = 16-SE 6.aif oF 14 ¥oo' 61 > 160 O99) ef> vo (yD aoa OSD (45) meet OS Lee 9g), Bute Papwernis. (from 5 -llb-$2), fun niny bythe, vo 59 e227 Dilu DILurion Spur, HO Ge a — st Q) {ies ptady - 230 020 oe 0 ale SO 34) AO Suet Wao. pit eS 7d. Bee 49D) Sup If BES a cae (3 ses 370 +333 ts Sup OL “to S. [mal © a BO, AVF +260 ~ ® Sup al M310 Mae i pe 21 ops ® Ehation rg serdik pele ee 449 2 23.7 : . 1g & @ hen RN as i 02M WY 49,241 EUs ® - “Ig, Wet eee 4g R22 1&7 @ ” wr: aan ” a me at 210 ou 4G 24) wie 262 GO", WH Ro ott, YE AIDC | @Q * Ble. madly o Sdy 2 49%) 1G 19 CD AT K Bisel xii $358 4 1284 C3) Hh pl al <. eeoatingt — ook k ug 4%221 C2 ® Mg ©.0Z2 wR °, 44 “LOW 1363 BY, Dee AA eee 3 ese 7 1,639 rae Os Oe a SO W037 V3 uN esumeé Gel, S-bee Se > Start féto Un\ts, Reh eG prot Sop FCO Sep & cL ‘Me EOE we tse teas ip b Be Sup 1060 a Eo la s 68 Sup uf ir b eke Ef Dips 106” 208 ¥ F-1g- 9% Dra Ly te. Wea ye ova M K Oo, ere _ 20 PM™ q 3% Am(<- 2o- vt), Gy mi 6 ae wt: Gt frarsh . per Cand hy TDQTp ia: OO fires Yr p Oe p a ee t } 213 | , i Ta dl ed) Unde rag prot a prt wl ae 26 CATS ae ae 27 1081 126 cs «~b4O., 4H Eey y,O rOUy 3 27 3 a VOM. a: eA 4 Daa hg 102g g ney ‘ +034 ‘4 3.4 +03) =e ‘93 1020 rea? ouy Cay Lina ts m4 uu gy 50 >, Ie Spi es ee | 24 -e eg: URE? Gt fit uC 2e tA net OOOO AOD 1 64 13% pi 7 ot ‘Route wy ey =} 8) 10.3 c Hilmoe Pg: 3 | : Sin C- 21-50 Final Gel Fractionation On Pamocnning, an +O 1 * 3 oh dike as of S- 16 - oe te change as noted. Aenea ow, WES eet oho a ae G-22-D Reg centri es 1. Sm, a e be oe dys mt Di lobes ts 2 49 ml. Lath ats mt. 0.01 N\ KuPO4 pH WY, basi [312g eysleine Het. HO C d.0018 Un. cystic: ), Bia pl. about Gale oe 4 Sa DB.1 Mt Hate p26 - SQ - p= 6.32 BX) it Ore 4 sae (ire Gk s 0:32 g1Ce Layee et BIND a Sees Se paps Aes, SO CID ei ey mr 8 5 22eO 1 Fig S&a ae ; Ais a. o6 en _ eo4 fit tao 24 omy prot fat £14. PRG! prot fmt. “Ada” B1aet Cus (POW, gel: (22, Lig fiat ks Tower Simin, eh cha: . 20 nin ~ hora alin ai ter ~ lout Hocetmm, re So Sup ‘po Se(2i3°) 2 $2.¢, 1: ‘ ; a 156s” _ By Gul 1Ot—7:50 — 7369 24Ee ~ wp eee 274 prot [o = Mn 3 ma prot fmt To Sup T add iia mt Cys" (P64). Gels 1 Os Above. | Sup TE = S3.8mE~— py sind (il?) wt RS ~ 101 2,50 260 ‘Joq 7) = vara reg [ot = hg my [md - Go Wet Add Bl mw qth Baska: Sop i > 64 wi. OR OO tee t ge 7 oa wa fa Spey pe Ge, I - EQute i S.7S m2 0.14 KPOy phb.o- le TO Susp = 20. TOteds : smirke 4c, = = 3.9mL, coo 200 i366 ~ ig | 260 Touts ‘) 85mg [A Ss Hee ma J Cruts 2 3-7fm2 ©. 101 MKPy phat ~ 6 es aug 20 € devas shen ‘Ei, Ty = 8: Bnd : (wee) 4 are, 0:01M KPoy pH 7.4. - Hebe sah ¢ 30 ia , ap Aw (s-22-5%) .~ a = UO Spa Fem /pe , 31K - Blue mathly é Wa, ca BS 8.7m 9 10 Ge TIE 0, 8-472 mR DO // ml: Phenol Proteins Qu! 40) After pH ad, \~20 SG) ETE a (Savse) undil /® Ed it b (5-2-5) vn dat /@ EL b (e256) vndit “@® C0 fra (s-ai-sx) het Diatyrzed Ae EQ it b (y-20-s«) und Gc ing Diaty zed 7 Edi a(s-lese) und Dian wd 4® Std, Plasma Alb a Gry | ( AL OiAS- 0:0) 0.0 0:02 g:0\S Ooze 1 01010 O:010 3 822 © Fada 6. Silo, Sree £22.92 nen). Ape 601M Cray PHAGE a1 SY [S22-se] O ce PG Bt wg ele ty [rt 0:38 Zin. 1 26S7>, 0408 0149 oe RUT go Be 0.44 ee 1097 O14Y 2% Ov 4es 39 die Od. HS Ouse 1208. «Gl 3% oye Bl a .O4/6 Oud VERe che 3 o%4 6.500 | 4 ee 47 Bo 12 ee as 29 ae Mlmoe. Sen Assa hs or“ Ca 3 (P04), Cel S te e, S- ao Bicad= 2 by & Sig. PI? /1,200 ISmaun. 29% C. \ 2 3 Yy S 6 | ¥ (2- t\iy}, C-1b + 300 X O.0IMK, HOO, 1350 X ADP sion (nd 107° X Atlee plH |-30 + 200 aa Ota }-2s De? (SO Soe Fic 1200 2 fue) Sop wt Pee 1200 - ae \eS Lup Tt ,»200 pe oars is | hee ost). EO th. : 200 es 104400 aa a | ca) Et fib 1209 - 08-7 300 YA) oe eae = 6.2) hte tne ee Ce ee 1244) 1 03 9,300 Si "T127) © Hid WV =t00 LOIS A OFS OPES or S POVST yaTN FP 270 YX Chins Wega | ER OM SS fb 204 1 eee | ee 2F 24 - Control 365° Hig TOb es. 1 eae uU3i Totelepm ineop U24U0 4619 SS6O 2020 3070 4740 pm eorp = 378 WIL eG NYT TY YY piiintfotena Seb Sbb 373 4o- 137 aia ‘Sy de Total vol> Si saab $3.87 64 gv .8 g. ¥ LIV30 Tothlunk 2940 2666 2010 28% 1210 187 Pohl paok U2) 33.4 123 THuKS. ot HAy 49.55. © 20% (Uk. Soa = H6.2 pie eet Lowntl actiatd As! Pale teal Pye + tees (2 Sim hi HO - pu= $795 (2°) ~All Gt. Mig OH = 1. Doak = p= 6. £3 Os) 4d O11 He sidan) pts 6.82 (9 Ohad | 6,33 () O22 yL 6.16 (yo, °°) 1,6 bad. id < ALN TiN) Te BANOO my Lk ph = ¢ : “a fay) ® £,+$ Ge Vt ada Lidell iL} ane HO - Abed Olly Ihe. - 0.05 Wh 6.6 (24.0%) Pati pes 0.03 6,32 0. est $90 Ou (12, wll BNAE tz 2dauh ~ ph: $96 Gad, Oo. Of anlo.tal H e S97 *slgek $-23- 5 pg 33 @ | Assays on Dialped Gel Aaahion sa 2* TH? Up m/proce | “ 3 4 s 6 > Q- My $-16°S&% «300 300. 1300) «BRO 300 + 800 am A 0:0 1M KHPdy ¥BSO ff (F890 VIS SO S50 ASO aS 3 SM Aofah m [et FON f ONS, | Oye CODE TOSS SOIC + PE ee. a OY Dg, S21 De ol oo 1200 =, /00 ret ne 1‘ lel0 oe 2 e Ob Sa-sy Dod ~ - ,200 = x ie eet o La S-he-v Dod fe % a 12.06 12D ZOO Ho 298.) 01S Qa CON ie ore Ie isto opin - Bly. d= 247 Sur. te 12 12 Pee ee 2g = (onrr= 3y eu eal: Pe Ieee ee ee Total pm L102 5°) 4000 $2920) 1040 (yee pen P (ncorp. 202 fl OO). RIB IS Lees PAMPid cor Heny. 7/ "POs a) Gl +Go juan Punrcorp|ry ene 222 wh Hig ye 2h Parnas shovid dialy ze vs cyshune ~ phosphate? p42 @ Preotamme ce. Sorabine S-2i-5@ ITA €@Cdat) 3,2m5/@ Jom, S = 13.5% "Be a doo a Ld: 303 | Cyell iors Bayle In. SO rk EAlen wen ex) . ! Vdd o@INNACC cold, UIT Bede Sn pa 3 pr 2 Snhy a) |. Add 2p psig fae Cosh pred ee C HAG. - Stir. tn (te 10. min: 3) Cate Age [0 Men. sisal ea/jdo,. * lao zoom) 4 @ _ Superncrout eM ORs mt ( Peat ores ) Mustard color ppih= Bb rows) Meas dentry’ Age Qs above. s 2Zanwkl AO (colorless): me 5) Maus tasd colo pre cpitalt : Add | 2.57 mh - 020 Yo. Most pale = pelde ut, Co lened Loud (Ce ) 6) | Gn h Ye 1% m), servall (2/1200 /0_ mens 34 rl ne oh epaleseaet Eni Natants Ver. Lgl pp =p o. s4 AS; ; Te ols week IS Ga uae S-lo-¢ Wye2 Clo) ad fot Gieod «ith 51 Fo Cine). Mhogycne pH 62 lie fot), L hrabpd lt ad, € Stncyec. Hite S*26-Se 32 anG 14 M4, Ss” 9 vie fe] 16 6. ng. fro, und. G14 ( ty?) 601 ir?) 230595" “2s 320ml. A wh sma amount Wate b cuspend’ Bar hi of 5a6 ot 160 as Con fans & Hie. Smart YL Add ine “Sat AS Sieh Sos slat ere es (Aidiecl 4 Sonal ptheen. 6 00S mk rm craig afer, ‘ab coe Ne Ae Sites eo 4) oak: Pree |, SSrk 60% sold A, bent 3! 10m Wash- 15 mt. Prt. talonvp « Lo be Whoo phoephals A 24: GF duiitiepale scent setuhon - 000m ~2 feo os os a Pomove OrOam af ake vofi. he gu Ae akan ta Wa 12.09 [lt = au lo. To above 14 mt add. 121 CaxC#ou), get oars Sie, 10 mun Con bri We to ME, Ler vahf @ (fo men, speed.) . . a N, To yellow SV p,. ag Ose mt Alomna Cr ont We abe [2 Stu \O Man, ne CA COA Ao vi, clear” lows SolUWwN- Wl b oH ~ Gry ( Brom Cres of pure k) ( Roemnve 0.02 Seen, | J c CNPO- Dtatre Ys Aonnny Oro1m k gq pl 7 isn e-66een with On KC eye heere: 4 Ct: te je se Start © PM PM Cipdraty duty fe 101 ot? 0 4D FO get 143z Jdvee en dhe wep | v- bese i & Stop Walysis Oe 2.00 bbs, baw. Dive Dn > Cccp | O22 5s LYS .a2amg pots 2 [olny © j 260 011307 BE Phenol Pro kus md: 4, 0 od . Bt roy [ple nog [¢ BR \ anle fai oO 00 adg 22 Sterne Plasm Cul) 2: Cry | o0r0” 0490 28 ao Fret Oralpredt S 27° he) : 601s” O.4es sch. 204 103% 10:S da \-s O,0 1” ou 360- ,30 063 100 undrialywd sorry Pe 250° 10 03] Jo. Sod vt eA , 0 10 aS / © Qe 263 , 220 ose N.0 : ng Bie 41 SP. bd (3B 0 0: 010% oye 179 cl. Ox ai zc We 0,020~ 4D” 20 pHa Sosy 19 : Fofal proken in-2r7- Ales. el mG. Tofer plofecn th Y/S P= ae) NS —_ Q- Ny Assays New @- mye §-29S¢ 0:0) MICH, PO, Get e 4 SP Finat vi 5 bg ho cpm = 0 To fel pnt pane buy q —_ he To Or Rn Va ?. frsed hig sie ofune ales cid: "70 dt 1-700 — 4-100 hae aly ud ay el) ~{100 blend : h frat epi tA corp eS [nt ene. % bea] ok - Total Xs M Toth & as ood PQ, 3S HiMoe | on PROTAMINE ~ AS Cefe) Sue? Bmt 1m Mas ph te bo acm Myke O16 d O10CM Velen 7 P32) 01.20 mé Chyd si “14S 10 Come )+ 3.70 KO Bla d= As? fo blepm "ed pave 16, 280 ! 2 3 ¥ .. 6 2 6.300 | 7 > K 6.300 - x 0.075 - ae 0.260 . (aoe > 30) 01100 - (oe -.5) 01050 wo | — h(cort.s) 01080 0.020 @100 (vor 5) woe OTS O22 0,225 9.255 OUI be xX 393 U7 | BST 1207. | FS 365° IbhY 3882 /160 Yur 76 + acy 4200 122760 442.0 2/60 EUS 2800 ‘ Us| Gils as 1a 085! ay. VIE ee: 1 SAO ase) aes: 1 234 224 39% SH] Me Quy BU Oi ey peat est Bio 4 ae ag Pom 14S gr pose 1H OG 1. 29,S) 206! gO) dhe cake [$00 1070 S16 S10 66 1186 SINGER | 5 q ; OE a &. ls-269% ey ee E $-27-5F | Sue Vom Step & (5-26 «) which hag wean, stpsad A gaan (cop 4 £0% AS) S-Owd | Aid 0.3: me sah OAS(O%), Bahwesk ho and a most of oD) hore daty ap peads ~ (nof vey firb.d) Arid G:12 po@ Mahe — (OAL for bids G- Stand. IS muon cofl. Agen fone: fuvyatt~ bp speasct Take vp pein O02 wl 001M Diy 4: Shetty yeh en Sip peel ey he See Bar, on oe. ‘ oo eh ag, = ST ye Ee “ Pade z AF wd S BPC yy Fh © inf sete : Ao. Haus 10! Cu S tera HL (e May 2900 , B00 300 ei ,: Lays 447 + ¢ fis top ped. ey sap ya dh 2, COiMCPd, «319 ‘4Ve eos Oy Cobre Dregs | anicy ah, Bcc Ad? sypas. ‘OTe Oye 2 BIE Chen pp ce 043 f ; ed ; P Af Ps C1 1BR oe oe ie Oi EP By aay 4 G4. Qe dea pel diatotioe > Athalon a 24 Cf. se ‘ Sem ty * ‘ . ‘ ‘“ an Fen s a i ‘ ,OF0 2 “ é ~DR eg, ] * ttealc- , ’ pas q waved nv oh *) j Seiad i at a . / ” . pn fe Neh fo MTS © 2. ae FORE, on Sapet tote E aes et ye nal PER Co H, O 70 “ ort ! oH J- 2.7- BE - 0.0 Wy oO. Sd. (tattid) bina ady ast wil waads Mig? Ls (Li tathaA ) dese. abe ee 588o™ (prob Fc Bods B34 >. Stee ~ contol ZO UY Os ee puqy afetep-ene 229 vo 2¥¢0 [4,009 prhon, LS Bide 7 ee? pn Poe afd 2 q§ eR ee jotat- vel Ord. 510 5.0 Job prot ce] ue $40 @ Assos on Protumunet Ges Steps Pq se. 2.4 Sl RE Tee fe- Miy (s-2a5y) 1 300 0.01 M KH, Pd, 0.350— . 7 ae diz t oir 0:07 ? Mai eee. Wide Ts yee il BRL re ata ‘ 650 bre — wed ~— 5-27 Dh ce pc ae mee ta et 6 OUR big Mawks S~o-0€: Diluke ¢ bios M ~ [us buf, pee /S"mum, a7 ee 14é adding Ou of Yo 4, Liehhav al Ud bWe=noo /7S 228 v1 1270 12gse AIR — Bothopomnd Gm /2/ /9/ 29% 357 10 0 + abot \O7, 11! 27% 334 jIz0 — i ee are Chae ame| ants Merclodtid, - en? Oey OFT OLFT TIES — Total tem VPP P01. Zest 3 C7 A2t20 = 1, pmo ids Pr 0097 O57 o.aS? 1320 |.O1 peer ohh BV4 25k bE $3.57 4 oe buy Ae 10% 4 Reg Ci mk Bertram fit Q, S.a-F Yee