Crielirouy on or

hiemorandum to Dill Brown, pesasdées conferen:es at aaleigh, feb. 14-16, 14962.
Project I: Dasnidi leat iy OF TUE CHROoUSU au COMPOUNDS PRAT GO  WRiBupe

TO ts TY OF RavUial CHa: ud RISGEUS.

A General pee oe, Project ry

VERE RRO

 

Q axamination of the By progeny derived from crosses of two races,
each exhibiting widely differing morphological and chromosomal characteri-
gatious.W nodecto thy elununataal aro.ct ery Te Wy Nosed larly.
2. The selected races for project Is
(a) Palomero Toluquefio with many knobs Poocammointre with no knobs.

(b) sapalote chico with many knobs es. »oeKnobless #lint.

% Detailed outline for Project I.
(1) Lo be done at Raleigh, Summer if62:

a). Sow 50 seed of Cacahuacintle on May 14, 1962

Sow 50 " " " "May 24, 1962
Sow 50 " " Palomero Toluquefio on May 10, 1962
sow 50 " " 4apalote chico on hey 10, 1962
sow 50 =" " U.s.Knobiess Flint on May 17, 1962
Sow 50" "om " " " May 24, 1962
Sow 50 " " " " " " May 31, 1962

(2) Collection of Sporoeytes from: abe

a}e 25 plants of Palomero Poluquefio, aaa examine before pollination time.

b). 35 " " Cacanuac:ntly, al exmine rapidly to find those plants

that have no knob or only 1 knob.

c). 35 4apalote plants. hake chromosome examination of several of them
before pollination time. vtherg, Suould be placed in deep freeze for
later exanirnaticns.

(3) Pollinations to be made:

a). Cross at least 20 plants of +alomero Toluqueno by knobless

Cacahuacintl¥ plants, using Palomero Toluquefio as the female in the cross,
b). Make the reciprocal cross with a few of the no-knob Cacahuacintle
plants.

c). Sib or self the remaining no-knob Cacahuacintle plants and cross
these to other Csgcahuacir.tle plants that have only one or two knobs.

dj). Cross reciprocally 20 plants of dapalote chico, includi:g those that
received early cytological examination,--by U.S.Knobless Flint.

e). Sib 4apalote chico plants and plants of knobless flint not used in

making the hybrid cross.

 

WINTER, 1962-63. ‘To be conducted in Florida

1). Select 5 ears produced by each of the two types of hybrid cross
outlined above.

2). Sow 2 rows of kernels derived from each ear (20-30 kernels per row)

3). “bserve the degree of diversity of plant characters among the plants
in each family.

4), Make Bib crosses between individuals within each family (The plants

each
to be selected for,sib crossf#¥ would depend upon ........?%)

 

SUMMER, 1963. ~o be conducted at Raleigh.
the procedures for examining the BP, progeny will be outlined after
Sib crosses
ears of the #) have been examined. <A conference will be scheduled to out-

line this.
Race

Arrocillo amarillo
Bolita
Cacahuacintle
Celaya

Vhalquefio
Chapalote

Cénico

Maiz Yulce

Nal-Tel

Palomero Toluquefio
Reventador

salvadorefio
de Costa Rica

Z4apalote chico

4apalote grande

Collection Number

 

Pue. 260 (original)
Oax. 180 (original)
Mex. 7a (derived)
Celaya II(derived)
Mex. 208 (original)
Sin. 6 (original)
Mex. 207 (original)
eto. 100 (original )
Yue. 7 (derived)

Mex. 210 (original ?)

Nay. 39 (derived)

C.R. 30 (original)
Oax, 179 (original)

Chis .236 (original)

Dates of Sporocyte yuma 2}

collectior® an . a
; Vhagingo , 1959 Zod Ape ikaed
6/22 to 6/23

6/26 to 7/1
6/18

7/6 to 7/8
6/22

6/29 to 7/6
611%

7/1

7/8 to 7/10
7/1 to 7/8
6/29 to 7/6
7/6 to 7/8

6/22 to 6/23
6/22 to 7/1
rileekun catts

© liwelly Qmawtly- ¢/r2- bes

(«) Nal-Teh - Caujpumur povlytin) — 7/7 Tho

@) Mntalmaoute - 6h (geet poe ay

G Chspalety blog Te (ogo wetl fod perllpluiny)
(o) Maye clulye TI) ( geod jal laf as )
Cy Crtter 6/19 -

(W) Keukater  él29- 7/6 ( olifoeeelt pain ‘

(5) qnpolite ¢ olin 6 [22-423 (ood Pip v iv (oer iol
Ww 8 fy euel bA2-Tl a yp do

© Ulf ! eupuc 6/22

G Chang tle-7/8

@ Arla bloe-7/)
(10) felsic Pol gant Th WE ‘\ ae il | ao ad

ae ah

| - — ey
Sula day, Younes re blg (oll Hoare
~ \ AC {\ il Y- Ie b f Le. . Oi Lead
@ \ 45 (arse ee — ot Yo Gove} :
: wet eee Mea = fre kd hes le - 7/7

COR 30 dugee
_ Project II, THe BPRecT V# K-10 Uk Piis DIGERIS PION JF P HENYAL

VEARAUT iS Ti F, PROGSAN Tes JeRIVED PROM RECTPROCAL UROSSiS OF B, Phan ds,

 

 

 

 

 

 

 

DSO Ra te a
1. Parental races selected: pred
a). hose carrying K-10: | , dywwo Lo
4apalote grande, hexican collection # Uhis. 236 Sawa °
yaad ; " " if Pp . .
(Arrocillo amarillo, # Pue. 260 GR, 30 eer,
(Bolita tt " # Vax. 180
# Celaya II)

Fe absatunaiy de tobe, Ruse

b). U.S.Knobless Flint.
2. Sow 50 kernels of 4apolote grande in Ralfdgh, hay 10, 1962
3. Vollect Sporocytes of plants and examine immediately to determine
presenc® or absence of K-10 in each.
4, Cross dZapalote Ie, Webbe K-10, by U.S. Knobless Flint, using JZapalote
chico as female parent in cross.

5. Do same with plants that do not carry K-10.

 

WINTER 1962-63. ‘fo be conducted in Florida.

1. Grow 4 familes (10 plants each) from kernels on ears produced by cross
given in 4. above.

2. Grow 4 families (10 slants each) from kernels on ears procuced by
cross given in 5. above.

5. Grow plants of U.S.Knobless #lint (2 plantings)

4, lake reciprocal crosses between plants in 1. above and those in 2,

above with the knobless Flint plants.

 

SUMMER,1963. +o be conducted at Raleigh.

1. Grow progenies prod ced by reciprocal crosses described above.

2. Examine tne diversity of plant tyves derived from the reciprocal
crosses. If strong diversity is exhibited among them, in the manner
expected of preferential segregation of segments of chromoso.es carrying the

knobs cortre@buted by sapalote Grande, examine Le determine if
4
LN

K-10 is present in paants BLHLAMKAXERXEXHXWKKENEXK of these families and
Ww
is absent from plants derived from families that do not show this scued

distribution of fkamxkyxxkeaxtsx parental traits.
to be atte & P sal
1 Beod ul. table Peat
bl Zagadre dives -— wwalouwe ey and Aveitele
B) For ocd pbcly heuer
w in faery Lawuti
r. lait & ha Way, t otto Lu fowalior an. UEAY
Oy Abeta — luo Fi cee OT phiahelG -y Lee | Uevre
ule my ‘¢ a

Tolecrttimst y tee.
1. We b Lael bu@ru for Abed) yout lhesLep Yo li aise Ib Beg fidty
a) eh longed (U8ee
a Ld Veaboau r

4%) Aen Ul Cerpbur aoe hun dy
Qed Yule Loos lea. t

2. Unite Runge fn atdef Moxey Many ~- nine, WED
(bial cay 4 Ctl C psec olla) quilbow? pjgelia
( | We pl) Veuu fp Wace ). G4 Uae Allied ¢ cobble (bef
Ropes tok laucwraloiur Veg ewe oat oc ba Weed OL
cclind  pooldeny (DoT ouatuy,, cur peel, lob fel Poll Wire |

eo poe [tuerug the yy ol anki vo elle ]
us Lester je "Af
5 Cleat ates fen Absa ab i oad whey.

1 baal: watin ov quality 4 Oliuritoe (ce pecee an 07) Colas 4