a, aie | Lady fo

The "A," State of ae

Cs
To illustrate pigmentaa patterns given by the Ao state of ao”

tang a \ 4,
folloWing changew in action of Spm.

I. 8720-8 x 8734D-3 Note:

1. Ratio of fully pigmented kernels to spotted and colorless
kernels = % colorless : & fully pigmented : % spotted.

2. The fully pigmented kernels received aot but no Spm from
male parent. ‘hese kernels are "wx".

3. The colorless kernels received a, from the male parent.
Half of these show glassy spots which represent mutations at
wxt-8 to an allele of Wx in response to the active Spm introduced
from the male parent. Those with no glassy spots did not receive
this Spm.

m-1

4, The spotted kernels received a, and andactive Spm from

the male parent. All of these kernels also have Wx sectors.

Tne pigmented spots represent, inactivations of Spm that occurred
during development or, losses of Spm geen transposition.

Note that many of the large pigmented areas have a twin area with
only tiny specks of pigment in then. These twin areas probably
reflect transpositions of Spm that occurred during development.
Mitotic segregation introduced two Spm into one nucleus and

resulted in loss of Spm from the sister nucleus. (For patterns

of pigmentation given by doses of Spm see sections 4, Fang ).
II. 8720-13, & Bt/ay bts wxt SB /wx™-8 x 8734D-3.
No Spm

1. Note linkage of a. with Bt, and absence of linkage of

either locus with Spm.
_ te
2. Details of patton of ao 1 and wx" n-8 to Spm and changes in

its action (or location) are the same as described in I.