Table 51

Spm constitution in progeny derived from the_Y, variegated class of
kernels on ear of the main stalk of plant 6665E~-10 (culture 6864) and
from the ear of a tiller of this plant (culture 6865)

Culture Number Phenotypes of kernels on
testcross ear
Pale Variegated
Y ¥ x y Total
I Spm, not linked
with ¥
6864 (11 plants) 1075 1113 1045 1036 4269
6865 (12 plents) 1023 1052 979 1026 4080
2 age Spm, m@ither
linked: with Y
6864-7 | 66 80 146 146 4A3B
686442 | 51 29 150 193 428 |
6865-1 68 54 165 17¢ 463
6865-12 39 “+0 113 121 313
2 Spm, one linked
with ¥
6864-—% 27 70 L160 117 315
Oe

2, Spm, one linked with y (?)

fF
Table 52

Proportion of pale colored to variegated k.rnels on the testcross
ears produced by the Pr/Pr plants in culture 6683 and 6685
entered inl, 2, and 3 of figure 3

Spm Constitution Number of Number of Phenotyves of a a
Plants Testcross carrying kernel’
Bors Pale Variegated Total
1 Spm 5 5 687 651 1338
2 linked Spm 5 7 694 1030 1724
3 Spm 1 3 170 593 763
Phenotypes of kernels on testcross ears produced by plants

Table 53

entered in 4 to 7 of figure 3

Spm constitution Number of Number of VPhenotyves of a

Plants carrying kernels

Pr + +/pr Spm Spm

Pr +/pr Spm plus
1 non-linked Som

Pr +/pr Spm
6683D~2 tiller ear”
6683D-3

6683E-8

6685F-3

6685F-4

6585G-3

6685H-5

6685H-10

Pr/pr; 1 Spm
6685H~24

16

BRON NN NOW OR

Testcross
wars Pale
Pr pr
20 1410 336
2 230
1 89 26
1 92 13
1 96 33
1 195 69
3 275 QL
1 90 33
1 80 31
1 176
1 101
1 85 122

m-1

_ Variegated
Pr pr
996 2018
95 238
180 lat
19 90
32 52
74 193%
67 214
28 96
23 70
4] 115
29 61
100 109

Total

4760
563

519

214
213
531
647
247
204
332
191

416

gab OR «

ari

*
45 pale-colored kernels in a "No Spm" sector on this ear were excluded from

the table.
Table 54
Phenotypes of kernels on testcross ears produced by plants

entered in figure 4

Spm Consitution Number of Number of VYhenotypes of a2 carrying

Plants Testcross Kernels
Ears Pale Variegated
. Pro pr Pr pr Total

Pr Spm/pr +
Hars of 6684D—1 ok 50 gO 91 48 279
Pollen of main
stalk, 6684D-1 2 80 188 L114 46 428
Pollen of tiller,
6684D-1 6 128 228 208 107 671
Pr Spm Spm/pr + +
6684D-2,Ear on main stalk 11 62 81 29 183

" ",Ear of tiller 22 73 93 34 222
6684D-3 1 5 43 54 22 124
6684E~—5 1 140 134 56 430
6684E-6 1 161 141 54 356
pr/pr; 2 linked Spm
6684A 1 83 106 189
6684H8-8 1 155 234 389
6684E-9 i 116 248 364
6684E-11 1 15k 222 37%
pr/pr; 1 Spm
6684E-2 1 246 235 481
6684E-3 1 145 162 207
6684E-4 1 134 140 274
6684E-12 1 159 163 322
6684E-13 1 233 222 455
6685E-15 L 192 218 410
Table 55

Spm constitution te Pr/pr progeny of tiller of plant 6683D-2 :..

 

Plant Number Spm Constitution Number of Phenotype of a a
in culture Ears carrying kernets on
6878 ears
Pale Variegated
Pr pr Pr pr Total
A-1, Har Pr Spm/pr + 1 61 129 99 4.0 329
Potlen noomoomoon 2 73 124 106 31 334
A-3, Ears Pr/pr; 1 Spm 3 393 140 143 676
A-4,Hars Pr/pr; 1 Spm 2 490 195 221 906
Pollen " " "oom 2 213 79 92 384
B-2, Ears Pr/pr; 1 Spm 2 152 145 120 123 540
Pollen " " —" 2 406 72 105 583
5-3, 4st ear, Pr/pr; 1 Spm 51 51 43 44 190
ain stalk
2nd ear, no" ae 35 17 17 69
main stalk
Tiller ear Pr/pr; 2 Spm 25 25 78 78 204
B-4, Ears Pr/pr; 1 Spm 2 140 131 117 117 505

B-1, Ear main Pr/pr; 2
stalk linked Spm 100 101 137 149 487

Tiller ear. Pr/pr; (1Spm)
_feficiency in pr clasg 70 40 61 39 210
Table 56

Spm constitution and location in progeny of plant 6685F-3

Plant Number and Number of Phenotypes of a m1 oarrying

Constitution Ears kernels on ear Percent of
Pale Variegated Recombinants
ee in variegated
Pr pr Pr pr Totals class
Pr+/ pr Spm
6685F-3, tiller ear 87 30 él 74 212 22
Pr Spm/pr +
6B82A-—4 1 55 201 177 Aa ATT 20
6682A+5 i 40 166 160 26 392 14.
6688A-7 1 45 14% 139 31 358 18
6882B-—3 1 9 40 44 12 95 O4
Pr/pr; 1 Spm
6682A-2 1 Be 83 94 92 351
6B824-3 1 154 141 88 9% 476
6B82A-6 1 “85 66 66 69 286
6882B-1, pollen 3 185 171 134 152 642
Pr/pr; 4 Spm
6682A-2,Ear on main stalk 15 14 235 PAG 510
Tiller ear 9 50 54 113

Pr/pr; No Spm
6882B-2
Table 57

Spm constitution and loc: tion in =a progeny of plant 6685G-2

Plant Number and Number of Phenotypes of a, 2?

carrying

 

Spm Constitution Ears kernels on ears
Pale _ Variegated

Parent Plant x mE =r Z
Br Spn/r +
6685G-2 1 132 0 137 0
Progeny,
Culture 6875
Pr Spm/pr +
A-4 2 75 296 274 7O
A-5 1 39 165 170 37
B-3 2 81 277 228 49
B-4" 3 77 345-305 69
B51 2 109 335-334 126
B-10 2 53 228 201 64.
A-1 1 191 160 2€
A-2 2 264 L77 49
A~3 1 184 126 20
A-6 1 266 230 4d,
A-7 1 25% 202 60
B-6 1 207 173 42
B-7, Tiller ear 93 69 il
B-12 1 167 160 33
B-13 1 173 132 46

Totals 3878 2941 756
Pr Spm/pr +; plus 1 Spm, .
B-9 1 49 105 209 148
B-7, Har of main stalk 128 132 87
Pr/pr; 1 Spm
B-8 1 281 114 132

Pr/pr; Inactive Spm, location not determined

B-l, B-2, B-ll

Totals

269

715
411
635
796
904
546

377
490
340
540
515
422
173
360
351
7575

511
347

527

Percent
Recombinants
in variegated
class

20
18
18
18
27
24

14
21
19
16
23
19.5
13.6
17
26
20.4

On the second ear of the main stalk there was a sector at the base of the ear
in which all kernels were pale colored in the upper part and in which variegated >
these being distributed equally between the

kernels appeared in the lower part,
Pr and pr chasses.
tmnere given for this ear.

waeer SaVS 81 BEG" Pode

n whi
a giv

The kernels within this twin sector were excluded from the

BLAS +g Ob eee SPE aga £OSmtthd hat

bp FRrgedar,
Table 58

Spm constitution and location in progeny of vlent 6684D-1. Spm

 

received from tiller of plant 6684D-1. Only one testcross ear obtained
from each progeny plant.
Som Sumber and location Fhenotypes of a carrying Recoubinants
in plants of cultures kernels on testcross ears amcng Variegated
6680 and 6681 Pale Variegated class of kernels
Pr pr Pr pr Total
Pr Spm/pr +
6880-1 57 148 137 53 395 23
"  =3 40 114 120 23 297 16
no LA 57 125 lel 52 355 30
"=5 53 192 172 58 475 25
"6 Al 131 118 23 313 16
N=7 75 161 166 73 475 30
"  -8 40 169 142 30 381 17
"  -10 26 63 69 17 L75 20
"  -11 32 71 96 37 236 28
Totals A421 1174 1141 366 3102 24.2
6881-1 198 106 48 352 31
iD) 175 95 34. 304 26
"m4 58 42 26 126 38
" -6 125 59 26 210 31
" =-7 77 45 21 143 32
"  -8 197 117 45 359 28
"9 201 117 37 355 et
"  -10 58 35 14 107 28
Totals 1089 616 251 1956 23.0
Pr/pr; 1 Spm
6880-2 67 73 71 77 288
6681-2 234 98 99 431
Pr/pr; 3 Spm
6681-5 31 36 167 176 410
No Spm
Table 59

Spm constitution and location in progeny derived from self-pollinated
ear of tiller of plant 6684D-1

 

Spm Constitution Phenotypes of a n-l oarrying Percent of
. kernels on testtross ears Recombinants
and Location in Variesrted
fale Variegated in varlesite
? class of kernels
Pr pr Pr pr Total
Farent Plant
Pr8pm/pr +
6684D-1, ear of
_ tiller, self-poll. 38 32 142 28 240
Progeny, Culture 6879
Pr Spm/pr +
6879-3 51 73 77 49 250 54
tay 13 36 53 16 118 23
"16 14 25 24 7 70 22
"  =-7 170 88 55 313 38
"  -18 147 94 28 269 24
T
Pr +/pr Spm otals 529 336 155 1020 31.5
6879-20 73 52 32 66 223 33
"43 138 46 79 263 35

Pr/pr; 2Spm (allelic positions ?)
6879-11 Ear, main

stalk 13 24 132 130 299
Tiller ear 5 12 73 76 166
6879-19 32 32 100 70 234
Pr/pr; 1 Spm
6879-5 27 18 19 17 81
"10 202 88 31 371
Pr/Pr; 1 Spm
6879-6 184 127 311
"12" 59 40 99
Pr/Br; 2 Spm (allelic positions ?)
6879-1 35 257 292
"214 Q3 249 342
No Spm
6879-9
%

Spm was inactive in a few kernels on this ear.
Table 60

Spm number in progeny of plants having either 1 or 2 or 3 non-linked
Spm efements in them. The Spm number given in this table is that which
was present in the part of a plant that produced the first ear on the
main stalk. Only those progenies that were grown in the summers of

1952 to 1955 are included.

Number of Spm Number of Plants in Progeny
in Parent Plant having 1, 2, or 3 Spm Elements
1 Spm 2 Spm 3 or more Spm Total
1 Spm 285 22 6 313
2 Spm 89 28 11 128

3 Spm 36 31 26 93
Table §1

Opn nunber..in ditfferent—p=rts eof individual plante. #&. Correspondence

of Spm number in all tested parts of vlant. B.. Nom=corréespondence of

We eh teeta Mae

Spm-number in aa part: of a plant.

 

 

“arts of Number of Plants with Stated
Plant Tested Spm Humber in Each Part Tested)
1? Spm 2 Spm 3 or mor? Spr Potat

Kars I and TL
main stalk 6 5 0 LU
Ear I, main etalk, wd 45 18 5 58
tiller ear
Ear I, main stalk, asd 13 3 1é
Pollen
Bars I and II, mein 3 4 1 8
etalk,*“tiller ear
Har I, main stalk, od 9 1 1 11
ears of two tillers:
Ears IT and JI, main stalk 1 1
and vollen
Kar J, main stalk, 8 1 9
ear of tiller, and
pollen
Har I, main stalk, 1 i
ears from two tillers,

ot, pollen

Totals 86 32 7 125

Onl plents grown in the summers of 195@ amd 19°55 are included in this
table.
Table 62

Non-correspondense of Spm number in different parts of a plant. Only
those plants that were grown in the summers of 1952 to 1955 are included
in the table. Parts of plants in which Spm was absent,or in which it was .

absent in a segment within the part tested are also exluded from the table.

Part of Plant Tested

Plant Number oem Number in 3 m Number in Spm Number in
ain Stalk iller Second Tiller

6452-3 3 2

6453-9 2 2 3

6676B-7 2 1

6684F-1 2 1

6872-2 2 1

6874B-—3 1 2

6874B-10 3 2

6875B-7 2 1

6876-8 1 2

6878B-1 2 1

6878B-3 1 2
Table 63

Yhenotypes of kernels appe:ring on testcross ears of vlants in culture 6888 that were derived
from uniformly pale colored kernels on ear of plant 66835B-2. Pollen parent used in making

cross was homozygous for ay and sh, and carried 1 active Spm.

Phenotynes of kernels on ear

 

Constitution Number of Pale Sh, Variegated Shy Colorless .Colé?téss
of Plant in Plants Many Spots of A, i or several Sh, ‘sh, Totals
Culture 6888 in colorless spots of A
background in colorless
background
a,8-tsni/a,Z sn, 2 419 ATL 1 0 0 891
2,7 Stef) Bao

a," sn,/a, sh, 5 596 597 1 1 1142 2337
Table 64

Phenotypes of kernels appearing on ears of plants in culture 6888 that were derived from kernels
on ear of plant 6683B-2 that had only 1 or several dots of Ay in a coloriess beckground. The

x

pollen parent used in making the cross was homozvgous for a> Sho, and pr and had 1 active Spm.

Csea Pique 53

Constitution of No. of
Plants in Plants
Vubture 6888

All were Pr/pr

m-L m-l
a Sh,/a,= Sho 2

" tt

m—1
a4~ Sh,/a, sh, 9

*
In addition, there were 3 sh

tetinn he

Pr

75

101°

2

£

Phenotyves of kernels on Ear
ale Sh, Variegated Sh |

 

 

a a he vast Gre artnet Sh
pr Many Spots of Ay l or several —2
in colorless Soots of A,
background in colorless
background
Pr pr Pr pr
102 170 177 97 56 31
91 131 118 52 43 AQ
169 283 256 163 67 5° 36

Colorless Colorless

SD,
Totals
0 708
Q 484
* y
1033 21083

kernels with many svots of Ay in a colorless background.
Table 65

Location of Spm-w in progeny of plants in culture 6888 2s judged

by kernel phenotypes appearing on testcross ears each produced. The

20Llen parent msed to make Bach cross

pr and it had no Spm.

1

m~-
was homozygous for a7 5 Sh end

] A —?2’
(state 5719A-1 or state 5718)

Phenotypes of Sernels on Ear

Constitution Number Pale Colored

of Tested of
Plants Hars
Pr pr
Pr Spm-w/pr +
Culture:
72620, D 5 206 769
7263C, D 5 298 782
7264B 9 431 1207
Totals 19 935 2758
Pr +/pr Spm-w
Culture:
7263) 3 AT5 203
Pr/pr; 1 Spm-w
Culture:
72630 2 170 166
Pr/Pr; 1 Spm-w
Culture:
7263C 2 284 --
fr Spm-w/pr +
Delayed tyme of
activation of Spm
Phant 7264B-6 1 142 154
“Inactive Spmewut 60 25

1 or several Colorless
A, dots in
colorless back-

ground
Pr pr Total
437 116 390 1918
404 138 457 2078
702 232 60°? 3174
1542 486 1449 7170
124 229 323 1354
76 88 192 692
143 -- 14° 569
30 8 85 419

85
Total 504
Table 66

Phenotyves of kernels on testcross ears of "No Spm-w" plants in figure 7.

Phenotype of kernel

Uniformly Yale Many A, Spots in

 

Plant Number pigmented ColorTéss background
YY ¥ “XY y Total
Y +Zy Spm-s :
726 2B-4 169 6 3 225 AO
7263A-1~ 9 13 6 20 48
"Aad 124. 24, 18 123 289
" A5 67 16 8 60 151
" A-6(ear I) 166 55 47 172 430
Homonc @ TT) 87 27 17 100 231
"AAT 149 59 Ay 137 389
Totals for plants in
Culture 7263 602 194 130 612 2538
¥ +/y Spm-s plus 1 Spm-s:
7263A-3! 135 28 81 194 438
¥ +/y Spm-s plus 2 Spm-s
7262B—2 51 3 152 193 399
v/s 1 Spm-s :
7263B-3 -- 159 - 162 321
" BAG -- 206 = 204. ALO
" Bag -~ 105 = 85 190
v/y; 2 Spm-s :
7263B~2 -- 107 -- 297 404

*
Ear was partially sterile.

Y, Pr

5
| One/kernel on ear had ony few A,dots in colorless backsround.

1
 

Table 67
Phenotypes of kernels appearing on testcrocss eurs of plants in 1. and 2. of figure 7 uncer

heading "Spm-w present".

Phenotype of kernel with Regard to

a, 2 action: Plant Pr ¥ or ¥ Pr y pr y Totals
“ale color (no Spm-s,no Spm-w) Number

7262B-1 28 51 0 3 82

" B-3 20 41 0 2 63

" B-5 18 54 2 2 76

7263A-8 32 54 7 5 98

Totals 98 200 9 12 319

7263A—2 42 37 79

Many A,spots in colorless
background (Spm-s present)

7262B=1 9 6 74 92 181
" Bes 0 5 74 66 145
t B-5 3 4 102 82 228
7263A-8 4 4 110 102 220
Totals 16 19 360 342 737
7263A-2 30 45 48 97 153
One or several A, dots in
colorless packeround
(Spm-w, no Spm-s) 7262B-1 56 24 2 1 83
" B-3 Al 15 4 2 62
" B-5 62 dL 1 4 T7
7263A-8 53 19 4 3 79
Totels 212 69 Ll 9 301
7263A=2 20 5 ll 5 41
Colorless; no A, dots
(Spm-w, no Spm-s) 726 2B-1 18

1 19

" B-3 3 0 3

" B=5 21 0 él
7263A—8 14 4 18
Totals 56 5 61
7263A-2 19 18 37

Total number of Kernels:
1728

Summaries of plants 7262B-1, B-3, B-5 and 7263A4-8:
737 Spm-s (376Pr : 361 pr; 35 Y : 702 y)
362 Spm-w; no Spm-s (223 Pr : 78 pr; 337 Y : 25 y)
319 no Spm-s, no Spm-w (107 Pr : 212 pr; 298 Y: 21 y)
29.8 % recombination between Pr and Spm-w

5.7 % recombination between y and Spom-s
Fhenotppes of kernels appearing on testcross errs of plant in 3 and 4 of

Table 68

figure 7 under heading "Spm-w present".

Plant Number

7260 43B=1
fart of ear
with Spm-s

Part of ear

with no Spm-s

7265-4

72635R-8
Tot is

7203B-7

Pale

L' 3C

17 42
204 fale

Phenotype of kernel

Many A, dots in
colorléss back-
ground
Pro pr
20 20
0 0
1S 44
52 58
117 122
183 elT

1 or several A.
dots in colorléss
background

Br
10
52
1G
29

107

30

pr

‘oO

25

li

Color-
less

ed

20

Totals

91

144

Lo4

228

627

552
Phenotypes of kernels anpe
state 5719/A-1 a

m-
1

Table 69

1

and were Pr “pm

ring on ears

ce

homozyzsous for state 57194-1 a2 _

Plant Bumber
of Female
p2rent in Cross

TB4T m2

734.7A-3,tiller-l
it tt -2

73474-4, tiller-1
"t " -?

7348A—-1,main e2r

" tiller e»-r

Totals

No Evidence of Spm:
Spm present: 764

xX
The number of Ay

of plants that

were homozygous for

m-w/pr + when crossed by plants

and pr that 1nd no Som in them.

Phenotype of Kernel

 

 

Pale Spots of A Solorless

Colored Solorless ae
ground *

Pr pr Pr pt total
46 110 79 50 4 P89
53 125 90 43 Ld 322
36 66 65 39 3 209
4.3 63 66 23 201
25 Alt AO 19 2 L131
37 AT 35 17 29 165
51 95 89 32 21 238
291 550 AB Ab 223 77 1695

841 34.6% are recombinunts

32.4% among variegated class are recombinants

spots mn most kernels was low,

being only 1 or several.

On the ear of plant 73474-2 one kermel apvveured with and oOpm-s type

viriegation pattern, and another kernel that had ne

Also, therewere

appe red.

sev

eral ke

within them

mrnels that head sectors ,in which many dots of A

arly as many dots of A

=1°

L
t

Table 70.

Phenotysoes of kernels appearing on ears of olunts in culture 7550/ tnat were
iu 1
Q.
=1
homosy.,ous for By and having no Spm («cart 1) or in which 1 opm—-S was present

(state 5700A)/ a, 2? (stacc S719:-1) proauee. oy Svous wath p.sats

(Part II).
Plant Number Position Culture Phenotyne of Kernel | x
vaso re of par + Number of Yale volored Variegated Kernel
- a etany Fl paren 57798-57008 Total
Ael First ear, 7510-14 178 , 0 0 178
main stalk
A=2 Second ear,
main strlk 7510-14 49] 0 0 4917
A-4 First ear,
main stalk 75%32D-4 549 0 0 549
Part II
A-~1 Second ear,
main stslk 75%32D-6 109 68 45 222
A~2 " " D-1 92 33 33 158
A=3 First ear,
main stalk " D-2 261 137 129 527
" Tiller ear " D-6 10 10 8 28
A=5 First ear,
main stalk " D=1 135 61 65 P61
" Second ear,
main stalk " D-6 86 50 38 L74
Totals 693 459 318 1370
* Url ‘ Leeds

Por state 5719A-1 kernels had many A, dots (see photo. ). #er state
5700A, the number of AL dots in a kernel waw fur in excess of thos under
A
heading 5719A-1 (see photo ).

Ww Shorus2s
Plant Number

in Culture
75308

Phenotyo:s of kernels anvearing on eurs of planis/th t were a

a, 2-1 (state 5719A-1), Br +/pr

Totals

1

Position
of Har
in Plant

First ear,
main stalk

Tiller

First esr,
main stalk

Second ear,
m3in stalk

First ear,
main sialk

tt

'f

homozyzous for ay and having no

Pale colored

243
150

Quad.

253
74,
265

1264

Table 71

YOM »

in culture 7530B

1

Phenotype of Sernel

Many A, spots in

1 or several Ay

colorless backgroud spots in color-

(state 5700A)

122
103

108
28

144.

39
115

659

less background
(state 5719A-1)

101
69

123
17

112
38
119

579

Colorle

ho
nm

ll

17

el

90

s

m-1 (state 5700A)/

Spm-w in constitution produced by cross with plants

Ss

Lotal

488
333

486
80

526

15q
520

2592
Table 72

Kernel types on ears of plants in culture 7529 that were ae (state 5700A) Sh,/ a, at (state
57194-1) Sho, Pr i/pr Spm-w in constitution when crossed by plants homozygous for a and sh,

and having no Spm. Yollen p:rent used in making cross to six of the eavs was homozygous for pr.

ww
VU

That used in making cross to 7#@ ear (first ear, main stalk, plant 7529-4) was homozygous Pr.

aby ee aks

Phenotyves of Kernels on Ear
Plant Number Pale Solored Small dots‘or specks 1 or 2 dots or Colorless
in Celture of A, (fromsé to mevaral specks of A, (st te
7529 hundred) in colorless in colorless 5719A-1)

beckeround (stzte backsround

57004) (strte 5719A-1) lotals
4, Ears I and II 369 180 95 83 727
5, Ear I 285 140 43 69 537
3, Ears I and II 221 108 40 77 436
2, Har I 293 33 17 34 377
1, Ear I 261 101 33 61 4.56
Totals 1429 562 218 324 2533

Totals, state 5719A-1:
542

of kernels huving Ay dots in eolorless backgroind

On evrs produced by cross with plants th t weve pr/or, 27.5%, were Pr and 62.5% were pr.

Sp pater a be oe
poy z c [ Fee ome

‘