Table 3l-a

Plants from C Bz - C bz, Sh-sh, Wx-wx kernels of 4892 x S sh bz wx, ds ac d

v
Dup. C sh bz Ax Wx Bz Sh Ds ac ac Q
Norm. C_ sb. bZ wx

 

 

nS ae
4A892B-1 4892B-2 4892B-3 4892B-5 4892C-1 4892C-3
Kernel type x x x x x x Totals
4804-21 4804-21 4804-21 4804-21 4804-7 4804-7

C Sh Bz 4x 310 186 261 172 188 128 1245
var. and non-var,
C sh bz Wx 4 2 4 1 0 1 12
C sh bz wx 334 205 253 132 216 117 1257

 

Totals 648 393 518 305 404 246 2514

 
Table 3l-b

Dup. C sh bz Wx Wx Bz Sh Ds Ae ace
Norm. C sh bz wx

C sh bz wx, ds ac @ x

4892 culture

 

4804-7 4804-22 4801-30 4801-32 4804-28 4803-48 Totals Grand

 

Kernel type x x x. x x x for totals
4892B-3 4892C-1 4892C0-3 4892C-3 4892c-3 4892c-3 4892C0-3.

C Sh Bz Wx 26 64 34 47 40 29 150 240
Non-var.
C Bz - C bz, Shesh, Wx-wx 35 46.. 37 53 45 29 164 245
(bz areas wx)
C sh bz Wx 2 3 4 2 1. 1 8 13
Non-var.

C sh bz wx 211 197 200 208 79 159 646 1054

 

Totals 274 310 275 310 165 218 968 1552

 
Table Slec

Dup. C sh bz Wx Wx Bz Sh Ds
Norm. C sh bz wx

ec sh Bz wx, ds ac? x ae ac 6

 

 

Kernel type 4785-82 x 4892C-6
C Sh Wx, non-var. 18
C-c, Sh-sh, Wx-wx Se

(ec areas wx)

C sh Wx, non-var. 1

CG sh wx, non-var. 197

 

Total 248

 
Norm. C sh bz Wx
Norm. CG sh bm wx

Table 32-a

Self-pollinated

 

 

 

7 7 4893-1 4893-3
ernel type selfed selfed tovets
C sh bz Wx, non-var. 129 90 219
C sh bz wx 35 o1 66
Totals 164 121 285

 
Table 32-b

Norm. C sh bz Wx
9 x C sh bz wx oc
Norm. C sh bz wx

 

 

 

Kernel type 4892p
4803-30

C sh bz Wx 245

C sh ba wx 234

Totals 479

 

Table 32-e

C sh bz wx 29 x € Sh_bz Wx é

 

 

C sh bz wx
4901G-6
Kernel type 7
4893-3
C sh bz Wx 124
C sh bz wx 138

 

Totals 262

 
Table 33-a

Bee.
C sh bz wx,ds ac? x Dup. C sh hz Wx Wx Bz Sh Ds

 

Norm. c sh Bz wx normal

Ae ac

 

 

4804-29
Kernel type x
4896
C Sh Bz Wx non-var,. 113
C BZ - Cc bz, Sh, Wx-wx 93
C sh Bz wx 195
C sh bz Wx non-var, 0
C sh bz Wx 38
Odds 0

 

Totals 439

 
Table 33-b

Dup. C sh bz Wx Wx Bz Sh Ds

 

9 x C sh bz wx ds ac oo
Norm. ec sh Bz wx (normal)

 

 

Kernel type 4896 x 4803-27
C Sh Bz Wx non-var,. 195*
C sh Ba wx 184
¢ sh bz Wx non-var. 3”
C sh bz wx 1?
Odds 0

 

* 42 were clearly C Bz - C bz Sh Wx-wx
+ 2 probably not cross-overs but due to deficiency
Supplement to, table 33

A

¥
C sh bz Wx Wx Bz Sh Ds

 

ec sh Bz wx da

Non~crossover gametes

Ac

Dup. C sh bz Wx Wx Bz Sh Ds”
t AN ac
Norm. c sh Bz wx as Ac and ac
(normal ) -
Region 1

Norm. C sh bz wx ds Ac and ac
Ac

7
Dup. c sh Bz Wx Wx Bz Sh Ds
Vac

Region 2

Norm. C sh bz Wx ds Ac and ac

vac
Dup. ec sh Bz wx Wx Bz Sh Ds
Nac

ec

ee ee

eta oi

71
= C Bz
= ¢ Sh
= ¢sh
= ¢ sh
='C Bz
= ¢ Sh
= ¢ sh
= C Bz
= ¢C Sh

0

kernel appearance

- C bz, Sh, Wx-wx dywrmed

Bz Wx

a b
BZ Wx = 195 184
ba Wx = $8 1?

-~ C bz, Sh, Wx-wx

Bz Wx

bz Wx = 0 1
prob.
- C bz, Sh, Wx-wx

Bz Wx
Table 34

C sh bz wx ds ac @ x I Ds Sh Bz wx Ac ac 6

C ds Sh bz Wx

 

 

4806-5 4B80C-3 4806-5
Kernel type x x x Totals
, 4883A-1 4883A-1 4883A-2
I wx , 72 76 80 228
Ipz-C Ba-C bz, wx 44 58 80 182
C bz Wx 139 178 173 490
I Wx - 27 29 14 70
C Bz wx 9 11 4 24
C BZ - C b2, wx i 3 2 6
I bz - C bz, Wx-wx 3 4 2 9
(bz areas Wx-wx)
I Bz - C Bz - C bzZ,Wx-wx 21 18 18 57
(bz areas Wx-wx)
Cc Sh bz wx 48 60 40 148
Others 2C Bz - 1 trans- 1 trans- 5

C bz,Wx-wx* posed De= posed Ds=
I Sh Bz Ds 1 Sh bz,
wWx-wx*

 

Totals 366 438 415 1219

 

* Probably from Ds mutation in div. to give sperm = loss of I
but not Bz.
C.0. I to Wx (excluding odds) = 25.8%
Supplement to Table 34

C sh bz wx ds ac 9 x I Ds Sh BZ wx gc ac g
C ds Sh bz Wx

1 2 3
I Ds Ba wx

C ds bz Wx
Non-crossovers

Ac = I BZ - C Ba =-C bz, wx = 182

I Ds Ba wx <

ac = I wx = 228
Cds bz Wx Ac + ac= C bz Wx = 490
Region 1
Ids bz Wx Ac + ac © I Wx

7 Ac = C Bu - © bz, wx = 6*

C Ds BZ wx \

ac = C Bz wx

Region 2
vac = Ibz- C bz, Wx-wx = 9
I Ds bz Wx \
ac = I Wx

C ds Baza wx Ac + ac = C Bz wx

Region 3

, Ac = I BZ =- C Bz -C bz, Wx-wx = 57

I Ds Bz Wx \
ac = I Wx

C ds bz wx Ac + se = C Sh bz wx = 148

 

Reg. 1 + §

i ds bz wx Ac + ac = wx

AQ = bz, Wxewx = 1
CG Ds bz wx © 3h bz,

I
C
ac = C bz Wx
Reg. 2 + 3
I

yac = bZ - C DZ, Wx
I Ds bz wx

7 ac = I wx

C ds Bz Wx Ac + ace C Bz Wx = 0

* Several of these probably from germinal losses of I in an
I Ds Sh Bz wx chromosome.
Max. c.o. Reg. 1 = 2%
Table 35

C Ds Sh Bz wx
Ac ac 6 (4883B)
C ds Sh bz Wx

C sh bz wx ds ac @ x

or

C Ds Sh Bz wx
Ac ae ¢ (4883¢C)
c ds Sh bz Wx

 

4804~13 4804-27 4801-1 4803-31 4806-2

 

 

Kernel type x x x x x Totals
4883B 4883B 4883C 4883C 4883C
C Bz Wx 12 20 16 14 17 84
Not obviously var.
C BZ - C bz, Wx-wx 15 18 25 13 16 87
(bz areas Wx-wx)
C Bz wx 61 70 81 56 50 318
Not obviously var.
C BZ - C bz, wx 60 57 51 44 39 251
C bz, Wx non-var, 123 158 119 124 122 646
C bz, Wx-wx 4 3 5 4 5 21
C bZ wx 33 52 29 22 29 165
Odds 0 0 2c sh 0 0 2
BZ wx
Totals 308 383 328 277 278 1574

 

Summary (minus odds)

740 Bz
838 Wx

832 bz
734 wx

°
*
°
e

Reduced numbers of Bz and wx due to:

Ds mutations in ¢ parent giving deficient chromosome 9
Supplement to table 35

Gemetes of 4883B and C

1 2
¥
Ds BZ WX ie ae
ds bz Wx
Gametes Kernel type
Ds Be wx ~ *° * C Ba-C bz, wx = 252
\ ac = C Bz wx = 318
ds bz Wx Ac + ac = C bz Wx = 646
Region 1
y Ac = C bz, Wx-wx* = 21
Ds bz Wx \
ac = C bz Wx
ds Bz wx Ac + ac = C Bz wx
Region 2
, Ac = C Bz - C ba, Wx-wx*e 87)
Ds Bz Wx \ |
ac = C Bz Wx = 845% Total 336 = 21.3%
ds bz wx Ac + ac = C bz wx = 165
*

Wx-wx from breakage-fusion-bridge cycles following a Ds mutation

giving a dicentric chromatid.

Crossing-over Ds to Bz =

Bz to Wx = 21.3%

=

Ds to Wx

 

5.2% Based on var. kernels
Non ¢.0. = 251

26.5% Reg. 1 = 21 = 5,8
Reg. 2 = 87 = 24,2
Total 359
Table 36

ec sh Ez wx, ds ac 2 C Ds Sh Bz wx Ae ae o

€ ds Sh ba wx

 

 

4785-21 4787-13
Kernel type x x Totals
4883B 4883B
C Sh Wx non-var. 128 142 270
C-c, Sh-sh, Wx-wx 20 18 358
(c areas Wx-wx)
C Sh wx non-var. 88 97 185
C-c Sh-sh wx 47 40 87
Odds 1 colorless Sh-sh, 1c sh wx 2
Wx-wx

 

Totals 284 298 582

 

308 Wx : 272 wx (minus odds). Reduction in wx class due to
Ds mutations in sporophyte of ¢d eliminating the C Ds Sh Bz wx
chromosome because of deficiency formation.

30.4% crossing-over Ds to Wx
Supplement to table 36

Gametes formed by 48838

C Ds wx
Ac ac
C ds Wx
Non=-crossover
y Ac = C-c, wx = 87
Cc Ds wx \
ac = © wx

Cds Wx Ac + ac = CG Wx

Crossovers
7 Ac = C-c, Wx-wx = 38 = 30.4% of variegated
C Ds Wx kernels
Nac = 6 Wx Crossing-over Ds to Wx

C ds wx Ac + ac = 6 wx
Table 37

q
e shwxdsac? x © DS SH WX Jo ae g

e ds Sh wx

 

4787-20 4786-74

 

Kernel tyne x x Totals
4883C 48836
tiller main stalk
G Sh /x non-var. 15 25 40
Cec, Sh, wWx-wx 1é€ 11 29

{ec areas ix-wx)

 

C Sh wx non-var,. 49 61 110
C-c, Sh wx 3o7 oe 69
e Sh J/x non-var, 77 lil 188
e¢ Sh wx o1 34 65

Totals 227 274 501

 

248 C 3: 253 ¢

*

257 Wx : 244 wx

29.4% crossing-over Ds to ix
Non cross-Ove

”
C Ds wx \

c ads Wx Ac +
Region 1

Cds Wx Ac +

ec Ds wx a

Region 2
7
C Ds Wx
\

c ds wx Ac +

Supplement to table 37

Gametes from 4883C C Ds wx

r
Ae
ac

ac

ac
Ac

ac

Ac
ac

ac

= C-c wx = 69
= © wx = 110
= ¢ Wx = 188
= C Wx

= ¢, Sh-sh, wx (difficult to classify)

ec Sh wx

C-c, Wx-wx = 29 = 29.4% of C-c kernels

C Wx about same as distance between
. C + Wx = 134 gametes in 501=
ec Sh wx 24.7%
Case I

Table 38

C Ds Sh Ba wx Ac ac 3

C ds sh bz wx

c sh BZ wx ds ac 2? x

 

 

 

4969-15 4785-23 4786-118 4785-8 4793-12
x x x x x Totals
Kernel type go, 4984 4884 4887B 4890-1
C Sh 131 96 63 89 104 483
Cec, Sh-+sh 86 85 96 80 102 449
C sh 207 197 141 192 174 911
Cec sh 1 7 4 5 4 el
1 Cec Sh 1 colorless
Odds 0 transp. Ds Sh wx* 0 0 6
1 colorless Sh* 1 pec.
2c sh wx C-c def,
Totals 425 389 306 366 384 1870

 

449 C-c Sh : 21 C-e sh = 4.5% crossing over Ds to Sh

* Due to loss of C in sperm from Ds mutation probably.
Table 39

 

ec sh a@x e sh Wx or ¢ Sh wx
Re e+ acac@? x Ke *—-—-—— +o
Re ++ Oe A@ ae
ec sh Wx € ds Sh wx

 

Kernel type 4793-17 4793-13 4793-14 4792-6 4793-7

 

 

x x x x x Totals
4882 4890D-1 4890D-1 4890D-~2 4890D-2
C non-var, 60 98 108 102 57 425
C=c, var. 65 68 94 92 85 404
ce 131 191 189 202 139 852
Odds 0 0 0 lc, Sh-sh, 1
Wx*
Totals 256 357 391 396 282 1682

 

* Probably from loss of ¢ throuzh Ds mutation leaving Sh in
chromosome with broken end. Sh-sh due to breakage-fusion-=
bridge cycle.
ec sh wx ds ac

2

Table 40

x
Re

ene

€

C

sh dx

Ac ac od

np

Ds Sh wx

 

 

 

Kernel type 4786-52 x 4882

C Sh wx non-var. 80
Cec, Sh wx 63
C-c, sh, wWx-wx 1
(c areas Jx-wx)
ec sh Wx 177
c Sh wx 1
e sh wx o

Totals 329

 
Table 41

ec sh wx ds ac 9 x. e—¢ ob Wx

1 eget ter cere

C¢ Ds Sh wx

Ac ace ¢

 

 

Kernel type 4785-4 x 4890D-l1
C Sh wx non-var. oo
Cec, Sh wx 32
ec Sh wx 80

 

Totals 147

 
Transvosed Ds 4628

Table 42

C sh bz wx ds ac @ x I Ds Sh Bz Wx Ac acd

C ds sh bz wx

 

5402-9 5402-10 5402-12 5402-14 5402-17 5403-67 5401-68 5403-71 5401-1 5403-10

 

Kernel Type x x x x x x x x x x

5492-1 5492-1 5492-3 5492-3 5492-3 5492-5 5492-5 5492-5 5499-6 5492-6
I Sh Wx 49 51 89 78 79 59 53 74 46 69
I-C Bz-C bz, Sh-sh 40 42 71 76 76 84 62 86 53 80

Wx-wx

{15 Bz 15 Bz 12 Bz 14 Bz 25 Bz 19Bz 29 Bz 198Bz 17 Bz
I Sh wx 15 20 14 21 15 34 20 42 28 29
I-¢c Bz-c bz-Sh wx 13 1? 12 14 16 27 30 36 29 23
I bz-C bz. Sh wx 1 0 1 6 1 3 1 2 1 0
I sh wx 4 2 4 10 7 5 6 11 4 9
I bz-C bz.sh wx 7 1 4 2 5 9 6 ? 5 2
Cc Sh Bz Wx 2 8 6 16 9 17 14 14 12 14
C Bz-C bz Sh Nx-wx 0 2 2 0 2 2 0 1 1 2
C sh Bz Wx 3 4 6 6 8 7 7 6 5 13
C sh b2 Wx 27 25 37 43 47 84 75 69 47 75
C sh ba wx 85 71 132 116 166 147 126 145 97 156
C Sh Ba wx 1 1 0 1 1 2 3 0 0 0

Odds 0 1cC¢ sh 0 1c¢ sh 1 1Ishic Bz-¢ bz 0 2I sh 1¢C Sh

Bz wx Bz wx Wx Sh, wx Wx bz Wx

1 I sh Wx 11 sh

1c sh Bz wx Wx

 
 

5400-20 5400-23 5400-50 5403-15 5404B-25
x x x x x Totals
54944A-1 5494A~-1 5494A=2 54944-2 5494A-2

56 85 36 .48 59 951
49 76 61. 85 76 1017
18 Bz 8421 Baz Yt Bz 5 Bz
16 24 22 15 6 321
18 19 24 17 17 312
2 2 3 1 2 26
5 4 2 2 4 77
2 3 7 4 3 67
4 3 6 1 11 137
0 1 0 1 0 14
5 2 2 4 § 83
24 60 47 34 44 | 738
101 158 138 96 141 1875
1 0 1 0 1 12

I sh Wx 21 sh Wxi1tIbz- QO 21 sh W#x cC sh BZ w -
c Sh bz c bz (1 var.) I sh Wx
wx sh Wx-wx

2
1

 
Table 43

e sh Bz wx, ds ac? x

I Ds Sh Bz Wx
Cc ds sh bz wx

Transposed Ds 4628

Ac ac ad

 

 

 

Kernel Type 5592-68 sete a _—* o502-25 oe 5892-57 5595-5 5592-56 5592-53 Totals
5492-1 5492-3 5492-5 5492-5 5492-6 54944(1) 5494A(2) 54944-2 5494A-2
Colorless Sh Wx 82 70 55 49 54 53 22 55 70 510
Colorless Sh, Wx-wx 80 67 54 Sl 42 Sl 29 85 81 540
Colorless Sh wx 53 31 49 41 47 47 6 48 32 354
Colorless sh wx 7 9 9 il 17 6 1 7 5 72
C Sh Wx 11 7 18 14 ii 8 3 7 2 81
C-c Sh. Wx-wx 0 1 4 0 1 0 0 1 0 7
(c areas Wx-wx)
C sh Wx, non-var. 54 42 80 65 52 40 10 59 53 455
C sh wx, non-var,. 143 122 96 110 120 112 46 105 153 1007
Colorless sh Wx 0 0 1 0 0 0 Q 2 3
Colorless sh @x-wx 2 1 0 0 0 1 0 4
Odds 0 0 0 lcshwx o 1} Cre Sh wx 0 0 2
of ¢
Totals 432 350 366 342 344 319 117 367 598 3035

 
Supplement to table 43

 

 

 

(1)(2)(3)
fi
I — Wx
C ds sh wx
Non-crossovers
wwe AC ™ colorless Sh-sh, Wx-wx 540
I Ds Sh Wx =~ ee, a
oe ~ ac = colorless Sh Wx 510
C ds sh wx, Ac and ac = C sh wx, non-var, 1007
Region 1
I sh wx, Ac and ac = colorless sh wx_
_~- Ac = Cec, Sh Wx-wx (c areas Wx-wx ) 7
C Ds Sh Wx — eee bs,
“~~~ ae = C Sh Wx, non-var, .
Region 2
I Ds sh wx, Ac and ac = colorless sh wx Ce Og

C ds Sh Wx Ac and ac = C Sh Wx, non-var.— gL

 

Region 3

I Ds Sh wx Ac and ac = colorless Sh wx (Ac = Sh-sh var.) 354

C ds sh Wx Ac and ac

C sh Wx, non-var. 455

 

 

Crossing over, regions 1 + 2 = 160 = 5.2%
" " region 1 = 7x4 = 28 = 0,9%
" " region 1, C class = 14

" " region 2, C class = 75