Lecture, February 1, 195h

Changes in state of Ac == the effects; Brakage-fusion-ebridge
cycles in endosperm; Transposition of Ds (Begin).

I, In previous two talks, discussed the disappearance of Ac from a known
location and its anpnerance at a new location -= transposition of Ac as
explanation given,

Also discussed the origin of changed action of Ae:

1. Double dose action of single Ac

2. Increase in action of that previously present but not doubled.

3, Decrease in dosage action -- one dose produces an earlier timed
break at Ds than that produced by Ac it replaced,

II, The changes in state: Besides (B), (2), and (3) above, have other
differences arising. these related to action of a dngle Ac or an
Ac in double dose: Control of the time of Ds breaks can be very
precise,

ITI. The relation of changes at Ac to those at Ds: A high rate of
coincidence, If a break occurs at Ds, then a chance at Ac is quite
likely to be detected,

IV. The nature of the patterns »roduced by doses of Ac and various states of
Ac: Begin Jan, 28 outline:

 

C sh bz wx ds ac female x I Sh Bz Ux Ds Acestabilized,

Photos: (& and Y,

V. The transposition of Ds,

1. How such transpositions detected: Those occurring from one
position to another in the short arm of chr mosome 9 easily found in
the tests with Ds,

2. If Ds moved from standard location to position between Bz and Wx:

a). The breaks: I Sh Bz Ds Wx 46

oun eeec

b). Ds break: vroduces a dicentric chromatid and acentric fragments:
Prophase:
I Sh Bz Wx
ae

I Sh Bz Wx

 

60

ce). The following anaphase:

|

—_— fy ~7
Uyg
Linky,

Q—-7
Break at (1)

4p +
—_—_

t
AM

The telophases

Next prophase and anaphase

Gu
Cm
Ly

"
a

. é
Mae

The types of sectors expected in the © sh bz areas:

 

3. If Ds moved to a position between I and Bz:

Ds break:

te y \

Yrophase —_—_

Anaphase of division following

The sectors:

 

Majority will be twins:

= By

Break at (2)

lus iby
ant Aan
wo pe
’ \
fh, \
Un 7
- “Poh
, . We.
thd . :
Ma HK
=
e

Wx with wx spots.

a BS i Ig Lye
UM
Pte ee ee

udder Cath
gu ot

sceber en P,

a Sg “sy

 
lh. If Ds inserted to left of I:

 

Ds I Sh Bz Wx
nan C—O
ZSWBy
oD C.. . Grn
. TSR, Wy
eX

| AY + Wl We
Toile: G42,

 

= = daa
VI, Analysis of a case of transposition of Ds from standard location to
position to baPbt of TI,
1. Cross in wiich detected: C sh bz wx ds ac female x I Sh Bz Wx Ds Ac
The kernels on the ear:

Table 1
Le

 

T Sh Bz wx Ds ac

The kernel showing Ds to left of TI:

Ds I Sh Bz wx Ac
ds C sh bz wx ac
including females, C sh bz, and either Wx or wx, ds, ac.

erossed to a number of tester plants

3. Plant from this kernel grown;
(a). Types of kernels on resulting ear:

Ds I Sh Bz Ac
ds C sh by ac
Table 2,

 

 

(b). Same plant to females, ec sh Bz wx ds ac:

Table 3.

 

ne

Types of kernels:

lh. The variegated kernels from crosses to C

hn bz Wx ds ac females
selected and vlants grown from them,
(a) Crossed to C sh bz wx ds ac females,
ear?

Table h,.

meena

ee

The types of kernels on
(b) Cross:d to c sh Bz wx ds ac females. “he types of kernels on
ear?

Table 5,
.
Tobe. @ ciqua A dean p saad D- 4u7s5C::

ea aa serrata

\. Crean : Cabl uncrree 2 t Lae Ws D,
© Sh Bz a, D

ale

2 Ryperouse 0) Morne on aan

L Ws, wou-tonr. = 102 |

= @
Tuy, Vou-ver = We 4

TW Ugh Ch uy duos = lou Dp B rade To gle Wy,
“ te =
T ty “ CH Aran = 54 4

=, S Kerwed, warh odd Whe 4 net gabon;

Koved Oppoaneuree . pally 2 plead fom Roruao:
2 sth LWtw wknd uy s a ae Vuploe . Cy /ThB, wed, /
Onearn append. Sry Chey TMB, wy fo.
ALetov oto .
- YA pred fw: Sh QO Wy
| Ten loy woh Cobbyilor-u — - ? mn
, rsh8 lon woh vordgelov oe Q
sia naling beale% Rb x = no prado ©)

iJ \ Tee any week ris gabon un
Aunteating lado tb Libba T = Vhs pred bo: Do IT Swhzay

(Teoma pass bo 4°75 6)
Ta be 2H wilaat af.
 

a abe
roll * (Raut us ss e- om genshowe }
Cabby Corman done x BENE fe oy Caen)
Ye x Looom rornmeds
© . .
Lenora, Willy Oe Chgrr ant. Ween O-)
ISh wou-var: 440 Gye tn anlar kegion @n 0c. -2 16
Ih wok CRs aan: 376 ( Wow- Crero-cnito wots Re ) Y
: i
he 7 Toh woh Chower  . aa
awh ; _t.-
“ Tn (4) ton-ver, (wath Vat quent, Nand wk Rennund to Weegee Uae eta is)
T Sah wiih Cdkoreo + 7
Wo 2 oa J) Sowudle :

C kh . 793 ( ve vn. ton be Ua -

C ShRy von- vor : 5S
: C SG wack Ca ortan : O

C aly won- Wr : iS

Total = VI35

S12 ©0 3 $63C.
T alxez

 

Table |
== eA Rpar rae x OTR fe (swoo-y a)
o, ©
¥
bh Ish A
pa day % Adin
« 4 d> C oh ou
( Vas . fo dw
eon neni ") Cab vo onto . Twu whee = 18
teas Dot ve Bale Peo z
r &_ = "7 Qik
C Sh, wen 162 7 16
CS ruth eaten = O
totekR C= 2323
Co, CS = Ib = 18%
Cio, Dot Tr 5 Wewinhe+ Idvok = Be = 10,77,
Tole 4

CNET n 4 - 3

Cabal 4 anoe 9 + Do I chRew, Reg
ao C ke

 

Won 7 Vou wor = 57H wou-voeL- = = ‘No
Cronos, TShRWe , Wri > uns Cdk We or. 64 5
Ca, ~€ t . = ab ao eT te
2 i owe ah
- = 4S
Regt . w 7 Wow wor Wow Wor, = 16
TAN WES Corin = 4a Ch Be ay
ChWe > ws Quer ‘Mr. =F o
& won = 34 wu-vers 43
4a TI te aa ¢. oY, an
Caster, wo rium » Wr =
Veta, ' '
Pa Wen vor = |63
43 TSR We yy, = \37 Calva = 311
Cited To Qapba T)
t.0
, Wow = Von-th = 1
+3 Tay Lan, -
‘ * por = 3 Ch BalOt ae, = Oo
(Ch, ows)
NON
C0: w N Won Wr 2 A
L4+3 TN iy Ch BS ek te 2 0

eS Tab Wwe Tota) C kerb = 108%

C.0. hh L = %.0'T “)»
rt Sh = §, 6%
Ont we = 20.7 Vo
Table 5

 

 

Aad By aggro $2 4 Do TShBgu, R gaz
do Cad low Oe

a
, ‘

20 ek, RK
on

 

XR MOwuy &
ay C oh we
T Mornatn ¢ Uferduts) —-@ Pewwats ( Coad 83)
yen - _ WoW = 1287
ae Tr wy = WeT C oh wy
6. o. . ‘ . = 68
Wot Roansti2d Gr dy van, Too Ayres Barerta t nate C
Wonton = 54
C0: - = 46
, Won VOU
© TBs en. zs YG C Shy = oO
OROAA Lan
dagu- Wr = 2149 tm-m = 390
” 7
@® I Shlvg . er. - 192 Cah oy. age = 3 er CoO + (
Oe o Ling ant Libya . |
x
Th uy 7 C sh los” Br = Oo
Cont cloanty for Do Rese
C pos reas = \ZuS
Coo. Q = GF 43571 KA = ure TID
Re) (Dey
Co. ® = T+¥= 100 = 5.4%

BQ0 +344 = 397 = 24%

oO
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