14th September, 1955 Dear Robley, Many thanks for your letter a manuscript, which arrived while I was away on holiday. I had heard quite a lot about the reconstituted virus, from various sources, and I'm very glad to have the details from you. I should certainly like to look at its X-ray diagram. The equator is completely different for polymerized A and for TMV, so it should be possible to get an idea of how much of the reconstituted has RNA down the middle. If it is reasonably easy to orientate, a pellet containing 3 - 4 mg of material would be enough to work with. The actual specimen used is, of course, a lot smaller than this, but it is usually necessary to try a good many times before getting satisfactory alignment. I am glad to see you are coming over for the CIBA conference next spring. Yours sincerely, Rosalind Franklin