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Laboratory diagnosis of diptheria. Part II, Determination of types of C. Diphtheriae
Laboratory diagnosis of diptheria. Part II, Determination of types of C. Diphtheriae
- Alternate Title(s):
- Determination of types of C. Diphtheriae
- Contributor(s):
- United States. Public Health Service.
Communicable Disease Center (U.S.) - Publication:
- [Washington] : Public Health Service : [for sale by National Audiovisual Center ; Atlanta : for loan by National Medical Audiovisual Center, 1951]
- Language(s):
- English
- Format:
- Moving image
- Subject(s):
- Bacteriological Techniques
Corynebacterium diphtheriae -- isolation & purification
Culture Media
Diphtheria -- diagnosis
Indicators and Reagents - Abstract:
- The purpose of this presentation is to describe and demonstrate methods of determining biochemical types of C. diphtheriae on chocolate tellurite plates, fermentation of carbohydrates and production of soluble hemolysins in broth. This objective is achieved in the step-by-step demonstrations of the test procedures. The characteristics of the four types of C. diphtheriae are described and demonstrated. In this presentation 0.3 ml. of each of the carbohydrates, glycogen 5%, starch 2% and dextrose 10% are added to tubes containing 3 ml. of bromcresol purple desoxycholate broth and incubated overnight. With a capillary pipette each of three tubes containing a carbohydrate and one tube of plain broth are inoculated with two drops of broth containing a culture of C. diphtheriae. A chocolate tellurite plate is inoculated with one drop from the same culture and streaked with a bent needle. All specimens are marked and incubated for 48 hours after which the colonies on the chocolate plate are examined for type and size. The 48 hour plain broth cultures are used in the hemolysin test. 0.5 ml. of plain broth is withdrawn from the tube, transferred to a sterile tube and labeled. 0.5 ml. of 2% washed human red cells are added to the culture tube which is shaken, put in a 37 degree C water bath for one hour, refrigerated overnight, and then examined for hemolysis. Hemolysin production is considered characteristic of the mitis type. The fermentation reactions are checked and the tubes returned to continue incubation for one week. The fermentation of starch and glycogen is considered characteristic of the gravis type. Failure to ferment dextrose is distinctive of the minimus type. The characteristics of all four types in culture are described.
- Copyright:
- The National Library of Medicine believes this item to be in the public domain. (More information)
- Extent:
- 012 min.
- Color:
- Black and white
- Sound:
- Sound
- Technique:
- Live action
- NLM Unique ID:
- 7600281A (See catalog record)
- OCLC no.:
- 61334595
- Permanent Link:
- http://resource.nlm.nih.gov/7600281A