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Some General Comments on Cloning and the Use of the P2 Room
Some General Comments on Cloning and the Use of the P2 Room
Contributor(s):
Varmus, Harold University of California, San Francisco. Archives and Special Collections. Harold E. Varmus Papers
To ensure the safety of recombinant DNA research (research that, among many other results, yielded important techniques for Varmus' investigation into the origins of cancer), the National Institutes of Health in 1976 issued guidelines for the construction and operation of laboratories in which such research was conducted. The guidelines established four containment levels, setting more stringent requirement for such features as air exhaust and wastewater disposal systems with each ascending level. This directive lays out the protocol for using and cleaning a level two containment facility in Varmus and Bishop's laboratory.
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