Human Lymphoblastoid Interferon: Purification, Amino Acid Composition, and Amino-Terminal Sequence

Human Lymphoblastoid Interferon: Purification, Amino Acid Composition, and Amino-Terminal Sequence

Contributor(s):
Zoon, K. C. (Kathryn C.)
Anfinsen, Christian B. (Christian Boehmer), 1916-1995
Zur Nedden, Dorothy
Bridgen, Pamela J.
Annals of the New York Academy of Sciences
Smith, Mark E.
Miller, Dorothea M.
Publication:
New York Academy of Sciences, 1980
Language(s):
English
Format:
Text
Subject(s):
Interferons
Amino Acids
Amino Acid Sequence
Lymphocytes
Genre(s):
Archival Materials
Articles
Abstract:
This article was the result of collaboration between various members of Anfinsen's lab at the NIAMD with Hunkapiller and Hood at Caltech. In it, they addressed the difficult problem of determining the amino acid sequence of Human Lymphoblastoid Interferon (HuIFN-alpha). Earlier attempts to sequence the amino-terminal portion of this molecule using conventional methods failed due to insufficient quantities of interferon. Through this collaboration, Anfinsen's lab developed a microsequencer, a technology that allowed them to obtain amino acid sequence data on 10 to 20 picomoles of protein. Because of this, they were able to successfully determine the amino-terminal amino acid sequence of the major component of human lymphoblastoid interferon (HuIFN-alpha).
Copyright:
This item may be under copyright protection; contact the copyright owner for permission before re-use.
Extent:
9 pages
NLM Unique ID:
101584571X177 (See in Profiles in Science)
Profiles in Science ID:
KKBBLM
Permanent Link:
http://resource.nlm.nih.gov/101584571X177
Archival Collection:
The Christian B. Anfinsen Papers (Profiles in Science)