Solid Phase Synthesis of a 42-Residue Fragment of Staphylococcal Nuclease: Properties of a Semisynthetic Enzyme

Solid Phase Synthesis of a 42-Residue Fragment of Staphylococcal Nuclease: Properties of a Semisynthetic Enzyme

Contributor(s):
Anfinsen, Christian B. (Christian Boehmer), 1916-1995
Ontjes, David A.
Proceedings of the National Academy of Sciences of the United States of America
Publication:
National Academy Press (U.S.), October 1969
Language(s):
English
Format:
Text
Subject(s):
Amino Acid Sequence
Chromatography
Peptides
Genre(s):
Archival Materials
Articles
Abstract:
After discovering an error in their original primary sequence analysis of staphylococcal nuclease, in which residue 43 was misidentified as glutamine instead of glutamic acid, Ontjes and Anfinsen published this paper. They reported that a resynthesis of the correct sequence in turn yielded a product with high biological activity. Therefore, they concluded that residue 43, although not essential for folding, was very important for catalytic activity once folding had occurred.
Copyright:
This item may be under copyright protection; contact the copyright owner for permission before re-use.
Extent:
8 pages
NLM Unique ID:
101584571X173 (See in Profiles in Science)
Profiles in Science ID:
KKBBLH
Permanent Link:
http://resource.nlm.nih.gov/101584571X173
Archival Collection:
The Christian B. Anfinsen Papers (Profiles in Science)