The Kinetics of Formation of Native Ribonuclease During Oxidation of the Reduced Polypeptide Chain

The Kinetics of Formation of Native Ribonuclease During Oxidation of the Reduced Polypeptide Chain

Contributor(s):
Anfinsen, Christian B. (Christian Boehmer), 1916-1995
Sela, Michael
Proceedings of the National Academy of Sciences of the United States of America
Haber, Edgar, 1932-1997
White, F. H., Jr
Publication:
National Academy Press (U.S.), 15 September 1961
Language(s):
English
Format:
Text
Subject(s):
Ribonucleases
Oxidation-Reduction
Protein Conformation
Genre(s):
Archival Materials
Articles
Abstract:
In this article, Anfinsen, Haber, Sela, and White reported that there existed a considerable lag phase before enzymatic activity appeared after the sample of bovine pancreatic ribonuclease was treated with mercaptoethanol in urea, during which period the sulfhydrl titer and the specific optical rotation changed along a curve similar to that of a first-order reaction. The lag in enzymatic activity in vitro often took several hours, while the same process seemed to take only a few minutes in vivo. This discrepancy eventually led to the discovery of an enzyme system in the endoplasmic reticulum of cells that catalyzes the disulfide interchange reaction, and subsequently, the rapid formation of the correct, native disulfide pairing in relatively short order.
Copyright:
This item may be under copyright protection; contact the copyright owner for permission before re-use.
Extent:
6 pages
NLM Unique ID:
101584571X167 (See in Profiles in Science)
Profiles in Science ID:
KKBBKZ
Permanent Link:
http://resource.nlm.nih.gov/101584571X167
Archival Collection:
The Christian B. Anfinsen Papers (Profiles in Science)