Fluorescence Studies of the Interaction of Nucleotides with the Active Site of the Nuclease of Staphylococcus aureus

Fluorescence Studies of the Interaction of Nucleotides with the Active Site of the Nuclease of Staphylococcus aureus

Contributor(s):
Anfinsen, Christian B. (Christian Boehmer), 1916-1995
Proceedings of the National Academy of Sciences of the United States of America
Edelhoch, Harold, 1922-
Cuatrecasas, Pedro
Publication:
National Academy Press (U.S.), November 1967
Language(s):
English
Format:
Text
Subject(s):
Binding Sites
Ribonucleases
Deoxyribonucleases
Staphylococcus aureus
Research Design
Genre(s):
Archival Materials
Articles
Abstract:
In the late 1960s, Anfinsen's lab sought to understand the complicated details of the physicochemical properties of Staphylococcus aureus in solution, ultimately to better understand the relationship of its structure to its function. In this article, Anfinsen, Cuatrecasas, and Edelhoch reported that the specific binding of a competitive inhibitor resulted in a marked decrease in tyrosyl fluorescence, which they interpreted as indicative of a specific interaction of the nucleotide with several tyrosyl groups in the active site of the enzyme.
Copyright:
This item may be under copyright protection; contact the copyright owner for permission before re-use.
Extent:
8 pages
NLM Unique ID:
101584571X150 (See in Profiles in Science)
Profiles in Science ID:
KKBBKF
Permanent Link:
http://resource.nlm.nih.gov/101584571X150
Archival Collection:
The Christian B. Anfinsen Papers (Profiles in Science)