A Study of the Factors Influencing the Rate and Extent of Enzymic Reactivation during Reoxidation of Reduced Ribonucelase

A Study of the Factors Influencing the Rate and Extent of Enzymic Reactivation during Reoxidation of Reduced Ribonucelase

Contributor(s):
Epstein, Charles
Goldberger, Robert E., 1937-
Archives of Biochemistry and Biophysics
Anfinsen, Christian B. (Christian Boehmer), 1916-1995
Young, D. Michael, 1944-
Publication:
The Academic Press, 1962
Language(s):
English
Format:
Text
Subject(s):
Ribonucleases
Disulfides
Enzymes
Peptides
Genre(s):
Archival Materials
Articles
Abstract:
In this article, Anfinsen, Epstein, Goldberger, and Young reported their findings of the rate and extent of return of enzymatic activity during oxidation of the sulfhydryl groups of reduced ribonuclease that they studied under a variety of conditions. They found that the rate of reactivation was inversely proportional to the concentration of the reduced portion of ribonuclease. Because of this, these results were inconsistent with the then-prevailing hypothesis that information necessary for the conversion of a newly synthesized polypeptide chain to the native form of the protein resided in the amino acid sequence and did not involve a genetically determined template.
Copyright:
The National Library of Medicine believes this item to be in the public domain. (More information)
Extent:
9 pages
NLM Unique ID:
101584571X143 (See in Profiles in Science)
Profiles in Science ID:
KKBBJW
Permanent Link:
http://resource.nlm.nih.gov/101584571X143
Archival Collection:
The Christian B. Anfinsen Papers (Profiles in Science)